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1.
Comparative effects of Shirazi thyme and vitamin E on some growth and plasma biochemical changes in common carp (Cyprinus carpio) during cadmium exposure
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Mohammad Mohiseni Maryam Sepidnameh Dara Bagheri Mahdi Banaee Behzad Nematdust Haghi 《Aquaculture Research》2017,48(9):4811-4821
Phytobiotics include a large number of active components which potentially have a growth‐promoting effects and antioxidant properties. This study was conducted to investigate the protective effects of Shirazi thyme and vitamin E on growth and plasma biochemical parameters of common carp exposed to cadmium. Fish (34 ± 3 g) were divided into four groups and fed four distinct diets including commercial diet without any additive (for control and metal only group) and supplemented with either 1% ground Shirazi thyme or 100 mg kg?1 vitamin E for 45 days. At the end of the feeding trial, all treatments except control group were exposed to sub‐lethal concentration of waterborne cadmium for 15 days and sampling was done on days 0, 7 and 15 after the metal exposure. According to the results, dietary inclusion of thyme prevented the harmful effects of cadmium and also improved fish growth and nutritional indices including weight gain, feed conversion ratio, specific growth rate and condition factor. Results also revealed that Shirazi thyme was more effective than vitamin E. Similarly, no changes in the hepatosomatic, viscerosomatic and bile somatic indices were observed. Plasma enzymes (AST, ALT, ALP and LDH) and metabolites were not altered due to thyme supplementation compared to the control. In conclusion, dietary supplementation of 1% ground Shiraz thyme improved the growth and health status of fish and showed better hepatorenoprotective properties than vitamin E during waterborne cadmium exposure in common carp juveniles. It might be conceivable to consider Shirazi thyme as a potential phytobiotic for incorporation in fish diet. 相似文献
2.
Bazinet L Gendron C Ippersiel D René-Paradis J Tétreault C Beaudry J Britten M Mahdavi B Amiot J Lamarche F 《Journal of agricultural and food chemistry》2002,50(23):6875-6881
A procedure developed for soybean protein precipitation which was based on electrodialysis was tested for the production of acid casein from reconstituted skim milk. In a previous paper, the performance of bipolar membrane electroacidification (BMEA) was evaluated under different conditions of ionic strength (micro(added) = 0, 0.25, 0.5, or 1.0 M) and added salt (CaCl(2), NaCl, or KCl) (1). The aim of this study, which is the complement of the work on evaluation of BMEA performance, was to evaluate the functionality of the protein isolates produced by BMEA and to compare the BMEA isolates to commercial isolates and an isolate produced by chemical acidification. It was not possible to show differences between the functional properties of isolates produced by BMEA, except at 1 M CaCl(2) micro(added), due to the variability of the isolates. However, the results showed that it is possible to obtain isolates similar to commercial isolates and that the addition of salt during the process does not induce variations in functional properties. From results on mineral concentrations, it appeared that the addition of monovalent cations did not influence the retention of monovalent or divalent cations in the BMEA isolates, while addition of divalent cations (CaCl(2)) influenced the retention of magnesium. According to previous results on evaluation of BMEA performances under different conditions of ionic strength and added salt, the difference observed for the BMEA isolate produced at 1.0 M CaCl(2) was confirmed. 相似文献
3.
Muhammad Amjad Javaid Akhtar Muhammad Anwar-ul-Haq Muhmmad Atif Riaz Zulfiqar Ahmad Saqib Behzad Murtaza 《Journal of plant nutrition》2016,39(13):1926-1935
To check the efficacy of potassium in alleviating oxidative stress under salt stress, salt-tolerant (Indent-1) and salt-sensitive (Red Ball) tomato (Lycopersicon esculentum Mill.) genotypes were exposed to three levels of sodium chloride (NaCl) (0, 75, 150 mM) and two levels of potassium (4.5 and 9 mM) in solution and foliar form. Thirty days of treatments revealed that increasing NaCl stress increased lipid peroxidation (malondialdehyde, MDA) and correspondingly the activity of antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; and glutathione reductase GR) in both genotypes. However, higher potassium (K) level in solution or foliar spray during the salt-induced stress decreased MDA and antioxidant activity and increased the growth in salt-tolerant genotype than in the salt-sensitive genotype. Decrease in MDA concentration, activity of antioxidant enzymes, and increase in the growth of tomato plants by the application of potassium under salt stress suggest that potassium is an effective ameliorating agent against salt-induced oxidative damage. 相似文献
4.
An experiment was carried out to assess the influence of tannic acid (TA) on integrity of the intestine in broiler chicks vaccinated against coccidiosis and challenged with the disease. In a 2 × 2 factorial design, the trial had five groups of 10 chickens each, including positive (group 2) and negative (group 1) controls. The chickens were kept on wood shavings and fed a commercial maize and soybean-based starter-grower diet. From day 1, groups 3 and 5 received TA (10 g kg(-1)) in their diet. On day 4, birds of groups 4 and 5 were vaccinated orally against coccidiosis with anti-coccidial vaccine, Livacox T?. Each dose of the vaccine contained 300-500 sporulated oocysts of each of Eimeria acervulina, Eimeria maxima and Eimeria tenella. On day 18, all experimental groups except for the negative (group 1) were challenged with 10-fold dose of Livacox T? to produce a mild coccidiosis infection. Faecal samples of individual birds were collected on day 23, and the number of faecal oocysts was determined. d-Xylose absorption test was also carried out on all birds on day 23. Immediately after d-xylose absorption test, all birds were killed humanely and the intestinal tract was removed, weighed and examined for gross lesions. Results showed that negative (group 1) and positive controls (group 2) had the highest and lowest levels of plasma d-xylose post-ingestion of the substrate, respectively. Vaccination and/or feeding TA raised the level of plasma d-xylose in infected birds, although this was not significant for TA-fed birds. Vaccination reduced but TA increased the total number of oocysts per gram of faeces. Birds of groups 2-5 had distinct intestinal lesions when compared with group 1. However, vaccination prevented intestinal lesions. Relative weights of intestinal parts were the lowest in group 1 and the highest in group 2. Vaccination but not TA reduced the relative weights of intestinal parts in infected birds. It was concluded that dietary tannins may reduce the efficacy of anticoccidial vaccines and alter the proper development of immunity against the disease. 相似文献
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6.
Semen characteristics of rainbow trout (Oncorhynchus mykiss) following diets containing different vegetable fatty acid levels
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M Hajiahmadian K Sarvi Moghanlou N Agh F Farrokhi Ardabili 《Reproduction in domestic animals》2016,51(6):979-984
Brood fish nutrition is an important factor susceptible to affect not only fecundity and gametogenesis but also gamete quality. In this study, we investigated the effects of altering dietary vegetable fatty acid content on semen quality (i.e. motility, density and seminal plasma composition), fertilizing ability and also blood testosterone (T) concentration in rainbow trout (Oncorhynchus mykiss). Fish were fed a commercial diet and ten formulated diets with similar proximate compositions but different levels of vegetable fatty acids (highly unsaturated fatty acids (HUFA): monounsaturated fatty acids (MUFA); HUFA: polyunsaturated fatty acids (PUFA); and HUFA: saturated fatty acids (SFA) ratios). Fish fed with HUFA: MUFA = 0.0 and HUFA: SFA = 0.25 ratios had the highest semen motility percentage and duration. However, the highest semen concentration and semenatocrit were observed in HUFA: SFA = 0.0 and HUFA: PUFA = 0.37 ratios. There was a significant difference in terms of K+ ion among diets supplemented with HUFA: PUFA = 0.0, HUFA: PUFA = 0.37 and HUFA: MUFA = 0.16 ratios (p < .05). Furthermore, Na+ ion showed significant difference between control group and diet supplemented with HUFA: PUFA = 0.0 (p < .05). Among the biochemical parameters, total protein showed a significant difference between HUFA: MUFA = 0.16 and HUFA: PUFA = 0.37 ratios (p < .05). No significant differences in fertilization ability and blood T concentration were found among dietary treatments (p > .05). In addition, the present data suggest that dietary fatty acid levels could affect semen quality but not fertilization ability in O. mykiss. 相似文献
7.
Anaesthetic efficacy of eugenol on Flowerhorn (Amphilophus labiatus × Amphilophus trimaculatus)
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Reza Tarkhani Ahmad Imani Hadi Jamali Kourosh Sarvi Moghanlou 《Aquaculture Research》2017,48(6):3207-3215
Anaesthetic efficacy of eugenol was investigated on Flowerhorn (Amphilophus labiatus × Amphilophus trimaculatus). A total of 104 fish with average weights of 12 ± 2.5, 28 ± 5 and 53 ±5.1 g were subjected to 25–200 mg L?1 eugenol and behavioural responses as well as induction and recovery times were recorded. Induction and recovery times were significantly affected by eugenol concentration as well as fish weight (P < 0.05). Generally, 49.9–127.3 s after exposure to 50–200 mg L?1 eugenol, fish reached stage 3 anaesthesia (suitable for general handling). Fish entered stage 4 anaesthesia (suitable for surgery and blood sampling) over 57.3–140.4 s post exposure to such concentrations. Recovery time was 91.7–312 s in all weight classes for all eugenol concentrations. Mortality (23%) was only observed in 12‐g fish when were subjected to 200 mg L?1 eugenol. This study showed the behavioural response of Flowerhorn to anaesthesia and eugenol efficacy as an anaesthetic in this important ornamental species. The general quadratic equation revealed that concentrations of eugenol and fish size along with their interactive effects have significantly contributed to the model, with concentration recording the highest beta value in all models (β = ?0.809, ?0.818 and ?0.909, P = 0.000). According to the results, minimum eugenol concentration to induce anaesthesia in less than 3 min was 50 mg L?1. 相似文献
8.
Seasonal Monitoring of Hydrocarbon Degraders in Alabama Marine Ecosystems Following the Deepwater Horizon Oil Spill 总被引:1,自引:0,他引:1
Agota Horel Behzad Mortazavi Patricia A. Sobecky 《Water, air, and soil pollution》2012,223(6):3145-3154
Following the Deepwater Horizon explosion and crude oil contamination of a marsh ecosystem in AL in June 2010, hydrocarbon-degrader microbial abundances of aerobic alkane, total hydrocarbon, and polycyclic aromatic hydrocarbon (PAH) degraders were enumerated seasonally. Surface sediment samples were collected in October and December of 2010 and in April and July of 2011 along 40?C70-m transects from the high tide to the intertidal zone including Spartina alterniflora-vegetated marsh, seagrass (Ruppia maritima)-dominated sediments, and nonvegetated sediments. Alkane and total hydrocarbon degraders in the sediment were detected, while PAH degraders were below detection limit at all locations examined during the sampling periods. The highest counts for microbial alkane degraders were observed at the high tide line in April and averaged to 8.65?×?105 of cells/g dry weight (dw) sediment. The abundance of alkane degraders during other months ranged from 9.49?×?103 to 3.87?×?104, while for total hydrocarbon degraders, it ranged between 5.62?×?103 and 1.14?×?105 of cells/g dw sediment. Pore water nutrient concentrations (NH 4 + , NO 3 ? , NO 2 ? , and PO 4 3? ) showed seasonal changes with minimum values observed in December and April and maximum values in October and July. Concentrations of total petroleum hydrocarbons in sediments averaged 100.4?±?52.4 and 141.9?±?57.5?mg/kg in January and July, 2011, respectively. The presence of aerobic microbial communities during all seasons in these nearshore ecosystems suggests that an active and resident microbial community is capable of mineralizing a fraction of petroleum hydrocarbons. 相似文献
9.
Sara Sadat Aghabozorg Afjeh Behzad Boshehri Safar Hamednia Asmaolhosna Asmaolhosna Parisa Mashayekhi Mir Davood Omrani 《Iranian Biomedical Journal》2021,25(3):220
Background:Methadone therapy is a major protocol in opioid addiction cases in many health care systems. Population-based studies have shown that in addicted people, the genetic profile affects their response to methadone therapy. Therefore, this study designed to examine the frequency of two SNPs of the CYP2B6 gene (rs3745274 and rs3211371) in addicted cases in two methadone-responders and methadone non-responders groups. Methods:A total of 199 opioid-addicted individuals and 117 unaffected control subjects were genotyped for rs3745274 and rs3211371 polymorphisms of the CYP2B6 gene using the tetra-primer ARMS-PCR. Results:Results of this study revealed the significant association of rs3745274 GG (p < 0.001; OR = 0.027; 95% CI = 0.14-0.49) and GT (p < 0.001; OR = 4.04; 95% CI = 2.26-7.21) genotypes with the risk of addiction in methadone-responders. Also, a significant association between rs3745274 GG (p < 0.001; OR = 0.28; 95% CI = 0.15-0.51) and GT (p < 0.001; OR = 5.1; 95% CI = 2.8-5.28) genotypes and addiction relapse was found in methadone non-responders. Conclusion:Based on our findings, we can conclude that rs3745274 variant of CYP2B6 gene could serve as a potential biomarker, to evaluate the prognosis of addicted people fate under treatment with methadone. Key Words: Addiction, Biomarker, Methadone, Single-nucleotide polymorphism 相似文献
10.
Kiani G Nematzadeh GA Ghareyazie B Sattari M 《Pakistan journal of biological sciences: PJBS》2008,11(4):648-651
Three transgenic rice varieties namely Khazar, Neda and Nemat, all containing a cry1Ab gene, were evaluated through PCR analysis and field examinations for their resistance at natural infestation of insect pests during 2007. The results showed that all transgenic varieties produced 1.2 kb PCR product derived from application of cry1Ab gene. In field conditions, transgenic varieties exhibited high levels of resistance against natural infestation of stem borer and the damaged plants based on dead heart or white heat for them were less than 1%. Moreover, in stem-cut bioassay 100% of released larvae died within four days after infestation. These results demonstrate that expression of cry1Ab gene in the genome of transgenic varieties provided season-long protection from the natural infestation of lepidopteran insects. 相似文献