Toxoplasma infection and abortion in dairy goats

Summary Serological investigation by the indirect haemagglutination test forToxoplasma antibodies was carried out in a flock of dairy goats. Antibody titres ranging from 1:4 to 1:1,024 were found in 34% of 371 apparently healthy animals. High antibody titres of 1:256 suggestive of recent infection were noted in 18 (4.9%) animals including 13 nannies. The occurrence of a number of non-brucella abortions associated with high serological titres and the isolation of the parasite from the brain tissues of an aborted foetus suggestedToxoplasma as a probable aetiologic factor. Sera having significant levels of haemagglutinating antibodies (1:128) were also found positive to the card agglutination test. Epidemiological aspects and the public health potential of subclinical toxoplasmosis in dairy goats are discussed.

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Infecciones Y Abortos En Cabras Lecheras Debido AToxoplasma

Resumen Se realizaron investigaciones serológicas de toxoplasmosis mediante la prueba indirecta de hemaglutinación en un rebaño de cabras lecheras. Se encontraron títulos de 1:4 a 1:1024 en 34% de 371 animales aparentemente sanos. Títulos de 1:256 sugestivos de infecciones recientes, se encontraron en 18 (4.9%) animales, incluyendo 13 hembras paridas. La ocurrencia de un significativo número de abortos no asociados con brucela, títulos serológicos altos de toxoplasmosis y el aislamiento del parásito del cerebro de fetos abortados, sugiere que eltoxoplasma es el factor etiológico más importante. Se discuten aspectos epidemiológicos de importantcia para la salud pública, relacionados con el potencial de la toxoplasmosis (subclínica) en cabras lecheras.

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Toxoplasmose Et Avortement Chez Les Chevres Latiteres

Résumé Des investigations sérologiques par la méthode de l'agglutination indirecte pour rechercher des anticorps toxoplasmiques ont été faites sur un troupeau de chèvres laitières. Des anticorps ont été mis en évidence chez 34 p.100 des 371 animaux apparemment sains à des titres allant de 1:4 à 1:1024. Des titres élevés d'anticorps atteignant 1:256 ont été relevés chez 18 (soit 4,9 p.100) des animaux comprenant 13 chèvres, ce qui fait penser à une infection récente.L'existence de nombre d'avortements non-brucelliques associés avec des titres sérologiques élevés et l'isolement du parasite des tittus cérébraux d'un foetus avorté suggerent que les Toxoplasmes en ont probablement été le facteur étiologique. Des sérums ayant des taux significativement élevés d'anticorps agglutinants (1:128) ont également été reconnus comme positifs par le test d'agglutination en EAT. Les aspects épidémiologiques et le danger potentiel pour l'homme de ces cas subcliniques de toxoplasmose chez les chèvres laitières sont discutés.

     

Colonization by Rhizobia of the seed and roots of legumes in relation to exudation of phenolics

A study of the colonization pattern of Rhizobium inoculated either in the soil or on to the seeds of green gram (Phaseolus aureus Roxb.), black gram (P. mungo L.) and sun hemp (Crotalaria juncea L.) revealed a significant reduction of Rhizobium in the spermosphere of sun hemp which was attributed to excretion of phenolic compounds by the seed. As the plants aged, the quantity of phenolics exuded by the roots was reduced and there was a parallel increase in the Rhizobium population in the rhizospheres of the three plant species.     

Epidemiological and bacteriological survey of buffalo mastitis in Nepal

A total of 355 Murrah cross buffaloes, consisting of 23 subclinical and 332 clinical mastitis cases brought to the Veterinary Teaching Hospital, Chitwan, Nepal from 2002 to 2005, were analyzed to determine the organisms involved, the seasonal occurrence of mastitis, and antibiotic susceptibility of mastitis pathogens. Coagulase negative Staphylococci (CNS) such as Staphylococcus albus and S. epidermidis were the predominant organisms associated with subclinical cases, and CNS and Coliforms in clinical cases. The maximum number (16%) of clinical cases of mastitis were observed in the month of July, when temperature and humidity are highest. The incidence of clinical mastitis was higher in animals during 1st calving and during the 1st month of parturition. Resistance to antibiotics was determined for 55, 23 and 149 isolates of Staphylococcus spp., Streptococcus spp. and Coliforms, respectively. In vitro drug sensitivity testing revealed that enrofloxacin had the highest average sensitivity (91%) for all types of bacteria. The effectiveness of other drugs detected were gentamicin (87%), tetracycline (83%) and chloramphenicol (82%). The antibiogram showed that both gentamicin and enrofloxacin are slowly becoming resistant. Mastitis pathogens have developed resistance to ampicillin and penicillin.     

An improved Lewis-Milne equation for the advance phase of border irrigation

Summary The Lewis-Milne (LM) equation has been widely applied for design of border irrigation systems. This equation is based on the concept of mass conservation while the momentum balance is replaced by the assumption of a constant surface water depth. Although this constant water depth depends on the inflow rate, slope and roughness of the infiltrating surface, no explicit relation has been derived for its estimation. Assuming negligible border slope, the present study theoretically treats the constant depth in the LM equation by utilizing the simple dam-break wave solution along with boundary layer theory. The wave front is analyzed separately from the rest of the advancing water by considering both friction and infiltration effects on the momentum balance. The resulting equations in their general form are too complicated for closed-form solutions. Solutions are therefore given for specialized cases and the mean depth of flow is presented as a function of the initial water depth at the inlet, the surface roughness and the rate of infiltration. The solution is calibrated and tested using experimental data.Abbreviations a (t) advance length - c mean depth in LM equation - c _f friction factor - c _h Chezy's friction coefficient - g acceleration due to gravity - h(x, t) water depth - h ₀ water depth at the upstream end - i() rate of infiltration - f(x, t) discharge - q₀ constant inflow discharge - S _f energy loss gradient or frictional slope - S₀ bed slope - t time - u(x, t) mean velocity along the water depth - x distance - Y() cumulative infiltration - (t) distance separating two flow regions - infiltration opportunity time     

Performance of agency-managed and farmer-managed irrigation systems: A comparative case study at Chitwan, Nepal

Management reforms are considered one of the best alternatives in increasing efficiency of the irrigation systems. Transfer of day-to-day operation and maintenance to farmers is not a new concept. However due to lags, even now it is a hot issue in developing countries and Nepal is not an exception. In this context of growing argument about the transfer of management of public irrigation systems to the Water Users' Group, this paper examines the comparative performance of farmer-managed and agency-managed irrigation systems. Khageri Irrigation System in the Chitwan district of Nepal was taken as the study site where complete management transfer has been done in 1996. The situations before and after complete transfer were compared. Intensive case studies of the selected farmers showed that equity in distribution of water and leakage had significantly improved after management transfer. Logit analysis revealed that age and education level of the household head, distance from main canal, leakage of the canal and equity in distribution are significantly related to the satisfaction level of the beneficiary farmers from FMIS compared to AMIS. However, landholding size of the respondent farmer has no significant effect. Similarly results from t-test revealed that rice productivity and overall profits from agriculture has also increased significantly due to irrigation management transfer.     

Genetic Analysis of Some Chemotypes in Ocimum basilicum var. glabratum

Screening of a population of Ocimum basilicum var. glabratum Benth., raised from seeds collected from France, West Africa and Kerala (India) was carried out for chemical composition of its essential oils. In all three collections, plants were found to be comprised of 3 chemotypes which were morphologically indistinguishable, but each chemotype was found to be capable of intercrossing freely with the other two. Selfing was carried out during 4 consecutive generations, after which three pure lines were established. Study of the selfed progeny of each collection exhibited an interesting segregation in S₂ and S₃ generations. Along with segregation in F₂ and F₃ generations of various hybrids the results can be explained by assuming a gene ‘M’ existing in 3 (or more) allelic forms: The allele M₀ for biosynthesis of estragole (methyl chavicol) is dominant to allelcs M₁ , for eugenol and M₂ for camphor. Also, allele M₁ is dominant to allele M₂ . The assumption was further confirmed by testcrosses of F₁ hybrids of each cross with their recessive parent.     

Genome-wide association study for spot blotch resistance in Afghan wheat germplasm

Spot blotch (SB), caused by Bipolaris sorokiniana, is a devastating disease of wheat globally, especially in South Asia and South America. Understanding the genetics of resistance to SB is important for developing breeding strategies to improve resistance. A panel of 301 genotypes from Afghanistan was phenotyped over two crop seasons using a mixture of virulent B. sorokiniana isolates and genotyped using DArTSeq to obtain genome-wide markers. Fifty genotypes (16.6%) showed disease scores less than the resistant control. Principal component analysis using the genotypic data clustered the genotypes into five different groups. Among models used for genome-wide association mapping, the multilocus mixed model, and fixed and random model circulating probability unification algorithms were most effective in identifying significant marker-trait associations (MTA). Twenty-five MTAs at p ≤ .001 were identified on chromosomes 1A, 1B, 1D, 2B, 2D, 3A, 3B, 4A, 5A, 5B, 6A, 7A, and 7D, indicating the quantitative nature of resistance to SB. Phenotypic variation explained by these markers ranged from 2.0% to 17.7%, and genomic regions on the chromosomes 1D, 2D, 3A, 3B, 4A, 5A, and 5B coincided with loci identified in previous studies. Three single nucleotide polymorphism (SNP) markers on chromosomes 1B (SNP 1113207) and 5A (SNPs 5411867 and 998276) were significant in both crop seasons as well as in the combined analysis across seasons. Marker 5411867 is close to Vrn-A1, shown to be associated with SB in previous studies. Furthermore, among known SB resistance genes, Sb2 on chromosome 5B was predicted to be significant in this panel.     

Effects of Partial and Complete Replacement of Synthetic Cryoprotectant with Carrot (Daucus carota) Concentrated Protein on Stability of Frozen Surimi

ABSTRACT

The study aimed to evaluate the effects of carrot concentrated protein (CCP) as additive on the functional and textural properties of surimi from striped catfish (Pangasianodon hypophthalmus) during six months of frozen storage (?20°C). The CCP (82.22% crude protein) was used as an additive either a lone or with a synthetic cryoprotectant (sucrose-sorbitol-sodium tri-polyphosphate). Control was made with synthetic cryoprotectant only. Molecular weight of CCP was found to be 36 kDa. After six months, the results revealed that up to 50% of synthetic cryoprotectants could be replaced by CCP during frozen storage of surimi. Biochemical parameters such as protein solubility, Ca²⁺ATPase activity, and gel strength decreased significantly (p < .05) during storage. Treatment T-3 (CCP 0.5% + 50% of synthetic cryoprotectant) maintained quality of protein significantly superior (p < .05) in respect to denaturation and other functional and sensory attributes compared to all the treatments. The microstructure images of surimi confirmed that addition of CCP modified the ice crystal growth during frozen storage. This study suggests that CCP can be a potential additive to protect protein from denaturation along with partial replacement of chemical cryoprotectants.     

Heat shock protein 70 expression in different tissues of Cirrhinus mrigala (Ham.) following heat stress

Members of heat shock protein 70 (HSP70) are highly conserved proteins of about 70 kDa and play important roles in protein folding. Levels of these proteins increase when cells are under stress. Environmental temperature influences both the basal and induced levels of HSPs. However, studies on HSPs in fishes from a tropical country such as India are lacking. In the present study, Indian major carp (IMC) Cirrhinus mrigala (Ham.) acclimatized at 25±2°C had high levels of HSP70, viz., 1.2–1.3 ng μg^?1 total protein in kidney and gill and 4.2–5.3 ng μg^?1 total protein in liver and brain tissues, indicating the presence of biochemically significant levels of stress. However, maintenance of acclimatized fish at 17°C for up to 48 h did not lead to a significant decrease in stress protein levels. A heat shock at 37°C for up to 48 h resulted in only two to threefold increase in HSP70 levels in these organs. Although the increase in HSP70 levels was apparent from the first hour of heat stress in all these tissues, the increase was significant from the second hour in the brain, the sixth hour in liver and kidney and the 20th hour in the gills.