全文获取类型
收费全文 | 325篇 |
免费 | 18篇 |
国内免费 | 1篇 |
专业分类
林业 | 24篇 |
农学 | 14篇 |
基础科学 | 3篇 |
41篇 | |
综合类 | 27篇 |
农作物 | 18篇 |
水产渔业 | 14篇 |
畜牧兽医 | 173篇 |
园艺 | 8篇 |
植物保护 | 22篇 |
出版年
2024年 | 2篇 |
2023年 | 6篇 |
2022年 | 7篇 |
2021年 | 15篇 |
2020年 | 12篇 |
2019年 | 14篇 |
2018年 | 16篇 |
2017年 | 14篇 |
2016年 | 12篇 |
2015年 | 6篇 |
2014年 | 19篇 |
2013年 | 20篇 |
2012年 | 27篇 |
2011年 | 37篇 |
2010年 | 19篇 |
2009年 | 4篇 |
2008年 | 13篇 |
2007年 | 24篇 |
2006年 | 15篇 |
2005年 | 9篇 |
2004年 | 12篇 |
2003年 | 9篇 |
2002年 | 11篇 |
1999年 | 2篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1973年 | 1篇 |
1966年 | 1篇 |
1962年 | 2篇 |
1949年 | 1篇 |
1937年 | 2篇 |
1929年 | 1篇 |
排序方式: 共有344条查询结果,搜索用时 15 毫秒
1.
Alice M. Evans 《Euphytica》1962,11(2):164-176
This investigation confirms the existence of a high degree of incompatibility between species of Trifolium. Two techniques were developed in an attempt to overcome the barriers to hybridization. The first deals with the use of grafting as a screening technique for compatible genotypes, and the second is an embryo culture method for salvaging hybrid embryos which would otherwise abort. The value of these methods is discussed, and the results are compared with those from hybridizations of non-grafted material and of seed set without artificial embryo culture. Hybrids were obtained from three species combinations at different levels of ploidy. 相似文献
2.
3.
4.
Landscape Ecology - Habitat fragmentation can exacerbate the negative effects of habitat loss for some species. Mitigating fragmentation is difficult, however, because population responses depend... 相似文献
5.
Bonaventure A. Omondi Misheck M. Soko Innocent Nduwimana Ronald T. Delano Celestin Niyongere Alice Simbare Donald Kachigamba Charles Staver 《Plant pathology》2020,69(9):1754-1766
The removal of infected individuals is a common practice in the management of plant disease outbreaks. It minimizes the contact between healthy individuals and inoculum sources by reducing the infectious window of contaminated individuals. This requires early detection and consistent removal at landscape scale. Roguing of mats with symptoms of banana bunchy top disease (BBTD) in Cavendish banana production systems has been tested in Australia, using trained personnel, but has never been tested in smallholder systems. We studied the effectiveness of long-term consistent roguing in prolonging the productivity of banana orchards under smallholder farming systems in highland banana and plantain dominated production systems in Africa. We assessed the possibility of low-risk seed sourcing from the managed plots. Roguing reduced BBTD incidence to 2% in managed farmer fields and to 10% in experimental field plots, while a nonmanaged field eventually collapsed in the same period. With roguing, new infections decreased monthly compared to an exponential increase in a nonmanaged field. The emergence of new infections in both managed and nonmanaged farms followed a seasonal cycle. BBTD managed plots were a source of low-risk seed for replacing the rogued mats in the same fields, but perhaps not safe for use in nonendemic areas. We conclude that it is possible for smallholder farmers to recover and maintain banana productivity with rigorous roguing, which would entail early identification of symptoms and early removal of diseased mats. Studies are needed on the intensity of roguing under different disease and production conditions. 相似文献
6.
Weber HA Zart MK Hodges AE Molloy HM O'Brien BM Moody LA Clark AP Harris RK Overstreet JD Smith CS 《Journal of agricultural and food chemistry》2003,51(25):7352-7358
The characterization of herbal materials is a significant challenge to analytical chemists. Goldenseal (Hydrastis canadensis L.), which has been chosen for toxicity evaluation by NIEHS, is among the top 15 herbal supplements currently on the market and contains a complex mixture of indigenous components ranging from carbohydrates and amino acids to isoquinoline alkaloids. One key component of herbal supplement production is botanical authentication, which is also recommended prior to initiation of efficacy or toxicological studies. To evaluate material available to consumers, goldenseal root powder was obtained from three commercial suppliers and a strategy was developed for characterization and comparison that included Soxhlet extraction, HPLC, GC-MS, and LC-MS analyses. HPLC was used to determine the weight percentages of the goldenseal alkaloids berberine, hydrastine, and canadine in the various extract residues. Palmatine, an isoquinoline alkaloid native to Coptis spp. and other common goldenseal adulterants, was also quantitated using HPLC. GC-MS was used to identify non-alkaloid constituents in goldenseal root powder, whereas LC-MS was used to identify alkaloid components. After review of the characterization data, it was determined that alkaloid content was the best biomarker for goldenseal. A 20-min ambient extraction method for the determination of alkaloid content was also developed and used to analyze the commercial material. All three lots of purchased material contained goldenseal alkaloids hydrastinine, berberastine, tetrahydroberberastine, canadaline, berberine, hydrastine, and canadine. Material from a single supplier also contained palmatine, coptisine, and jatrorrhizine, thus indicating that the material was not pure goldenseal. Comparative data for three commercial sources of goldenseal root powder are presented. 相似文献
7.
Kelly C. Nunes Cinthia Eyng Paula T. M. Pintro Rodrigo G. Garcia Alice E. Murakami Ana C. P. Vital Ricardo V. Nunes Patricia O. Nesello 《Journal of animal physiology and animal nutrition》2019,103(1):64-71
The aim of this study was to evaluate dietary supplementation of different levels of dehydrated bocaiuva pulp (DBP) on the productive performance of laying quails, and on lipid oxidation, and quality in their eggs. A total of 120 quails were distributed in a completely randomized design with six treatments (0%, 0.1%, 0.2%, 0.3%, 0.4% and 0.5% of DBP), five replicates and four birds per experimental unit. To evaluate lipid oxidation, the eggs were collected on five consecutive days at the end of the trial and distributed in a completely randomized experimental design in a 6 × 5 factorial scheme (eggs from quails fed different levels of DBP × evaluated fresh and after 7, 14, 21 and 28 days). The antioxidant potential of diets was improved (p < 0.05) when the DBP was added estimated as 2,2'‐azinobis‐3‐ethylbenzothiazolin‐6‐sulfonic acid (ABTS) free radical scavenging capacity. No effect (p > 0.05) was observed on feed intake, hen‐day egg production and feed conversion (kg/kg and kg/dz). There were no changes (p > 0.05) in the egg weight, Haugh unit, yolk index or eggshell percentage. Although no difference was observed (p > 0.05), when comparing each level with the control, a negative linear effect (p < 0.05) on specific gravity was observed. In addition, only the treatment with 0.5% pulp exhibited lower (p < 0.05) eggshell thickness compared with the control (0%). Thiobarbituric acid reactive substances (TBARS) analysis showed that as the dietary pulp level was increased, malondialdehyde content decreased linearly (p < 0.05) in the egg yolks, regardless of the storage period. Inclusion of DBP in the diets had no effect (p > 0.05) on the concentration of conjugated dienes in the eggs. The diets containing DBP showed a potential antioxidant effect, reducing the degree of lipid oxidation in egg yolk. However, no beneficial effects were observed on performance and egg quality. 相似文献
8.
9.
Prevalence of antibodies to bluetongue virus and Anaplasma marginale in Montana yearling cattle entering Alberta feedlots: Fall 2001 下载免费PDF全文
Van Donkersgoed J Gertonson A Bridges M Raths D Dargatz D Wagner B Boughton A Knoop D Walton TE 《The Canadian veterinary journal. La revue veterinaire canadienne》2004,45(6):486-492
A serologic survey was conducted in yearling cattle imported into Alberta feedlots from Montana during October 2001 to estimate the prevalence of antibodies to bluetongue virus (BTV) and Anaplasma marginale in Montana yearling cattle. The apparent prevalence of antibodies to BTV when the competitive enzyme-linked immunosorbent assay (cELISA) was used was 0.37% (21/5608). Test positive cELISA samples were also all positive when tested by virus neutralization (VN) and they reacted to 1 or more BTV serotypes, including 2, 10, 11, 13, and 17. The apparent prevalence of antibodies to A. marginale when a recombinant cELISA (rcELISA) was used with a positive cutoff at 30% inhibition was 1.93% (108/5608). When the rcELISA positive cutoff was at 42% inhibition, the apparent prevalence was 0.73% (41/5608). After the reported sensitivity and specificity of the test had been accounted for, the A. marginale antibody results were consistent with a population that was either free of exposure or had a very low prevalence for A. marginale. 相似文献
10.
Kahn S Geale DW Kitching PR Bouffard A Allard DG Duncan JR 《The Canadian veterinary journal. La revue veterinaire canadienne》2002,43(5):349-354
Vaccination of susceptible animals against foot-and-mouth disease (FMD) is a well established strategy for helping to combat the disease. Traditionally, FMD vaccine has been used to control a disease incursion in countries where the disease has been endemic rather than in countries considered free of the disease. In 2001, the use of vaccine was considered but not implemented in the United Kingdom (1), whereas vaccine was used to help to control FMD in The Netherlands (2,3). Canadian contingency plans provide for the use of vaccine; Canada is a member of the North American Foot-and-Mouth Disease Vaccine Bank, which could supply vaccine if needed. This article explains why Canada might use FMD vaccine to combat an outbreak and the factors that are relevant to the disposal of vaccinated animals and their products. It concludes that vaccination is an important mechanism in Canada's preparedness for an outbreak of FMD and that products from vaccinated animals are safe for human consumption. 相似文献