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Veterinary vaccines are usually tested for the absence of contaminants. However, the quality control does not always imply that vaccines are not contaminated as, for example, illustrated by the bovine herpes virus 1 (BHV1) vaccine used in The Netherlands in 1999 that contained a small amount of bovine viral diarrhoea virus (BVDV1). Thousands of cows were vaccinated with BHV1 vaccine batches, and the question arose as to whether these small amounts of BVDV1, most likely not detected with in vitro tests, could have infected cattle. More in general, the question was whether the outcome of the in vitro tests, i.e. the in vitro infectivity, was indicative for the infectivity for cattle, i.e. the in vivo infectivity. We therefore carried out in vitro experiments to determine the sensitivity of a BVDV1 isolation assay. In addition, we performed two animal experiments, in which we estimated the lowest dose needed to infect calves with BVDV1. We extrapolated the experimental in vitro and in vivo results from a tissue culture infectious dose (TCID50) to a cattle infectious dose (CID50). We observed a partial response in the calves inoculated with this dose: four out of six calves turned out to be infected. In the tissue culture test, all 20 samples tested negative. The response in vivo, however, was not significantly higher than the in vitro response, which implies that no difference in susceptibility was observed between the animal test and the tissue culture test. Based on the results in our experiments, some cattle may have been infected with BVDV1 after the application of the contaminated BHV1 vaccine during the vaccination campaign. The question remains that how many cattle received contaminated vaccine, and became infected with BVDV1. 相似文献
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Selhorst T Freuling CM Blicke J Vos A Larres G Zimmer K Fröhlich T Bätza HJ Müller TF 《Berliner und Münchener tier?rztliche Wochenschrift》2012,125(5-6):191-196
In 2005, the final phase of terrestrial rabies eradication in Germany was put at risk by a severe setback due to re-introduction of the disease in Rhineland-Palatinate from neighbouring Hesse after seven years of absence. The rapid westward spread of the disease prompted veterinary authorities to react swiftly and apply a new yet unproven vaccination strategy to rapidly increase herd immunity in an almost unprotected fox population to stop the epidemic. The cornerstones of this emergency oral rabies vaccination strategy, i. e. vaccination intervals, identification of high risk spots, real time epidemiological assessment, capable to eliminate rabies within 13 months after incursion are described here. This strategy may be used as a template to tackle similar emergency situations in Europe in the future. 相似文献
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Müller T Freuling CM Gschwendner P Holzhofer E Mürke H Rüdiger H Schuster P Klöss D Staubach C Teske K Vos A 《Berliner und Münchener tier?rztliche Wochenschrift》2012,125(5-6):197-202
Large-scale oral vaccination of wildlife against rabies using aerial bait distribution has been successfully used to control terrestrial wildlife rabies in Europe and North America. A technical milestone to large-scale oral rabies vaccination campaigns in Europe was the development of fully-automated, computer-supported and cost-efficient technology for aerial distribution of baits like the SURVIS -system. Each bait released is recorded by the control unit through a sensor, with the exact location, time and date of release and subsequently the collected data can be evaluated, e.g. in GIS programmes. Thus, bait delivery systems like SURVIS are an important management tool for flight services and the responsible authorities for the optimization and evaluation of oral vaccination campaigns of wildlife against rabies or the control of other relevant wildlife diseases targeted by oral baits. 相似文献
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Un H Eskiizmirliler S Unal N Freuling CM Johnson N Fooks AR Müller T Vos A Aylan O 《Berliner und Münchener tier?rztliche Wochenschrift》2012,125(5-6):203-208
Following a sustained spill-over event from dogs to foxes, fox rabies spread rapidly in the Aegean region, Turkey. In order to control the outbreak a program of oral vaccination of foxes against rabies was introduced. In the selected vaccination area three annual campaigns between 2008 and 2010 were undertaken during the winter months whereby the vaccine baits were distributed exclusively by plane using a density of 18 baits per km2. Subsequently, fox rabies cases were reported only from locations bordering the non-vaccinated areas. Hence, it was shown that fox rabies control by means of oral rabies vaccination is feasible in Turkey. However, for the progress towards the elimination of fox-mediated rabies in Turkey to be maintained, it is necessary that political and financial support is secured to extend oral vaccination where infected foxes remain. 相似文献
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Tessa Mazor C. Roland Pitcher Wayne Rochester Michel J. Kaiser Jan G. Hiddink Simon Jennings Ricardo Amoroso Robert A. McConnaughey Adriaan D. Rijnsdorp Ana M. Parma Petri Suuronen Jeremy Collie Marija Sciberras Lara Atkinson Deon Durholtz Jim R Ellis Stefan G. Bolam Michaela Schratzberger Elena Couce Jacqueline Eggleton Clement Garcia Paulus Kainge Sarah Paulus Johannes N. Kathena Mayya Gogina P. Daniël van Denderen Aimee A. Keller Beth H. Horness Ray Hilborn 《Fish and Fisheries》2021,22(1):72-86
Bottom trawl fishing is a controversial activity. It yields about a quarter of the world's wild seafood, but also has impacts on the marine environment. Recent advances have quantified and improved understanding of large‐scale impacts of trawling on the seabed. However, such information needs to be coupled with distributions of benthic invertebrates (benthos) to assess whether these populations are being sustained under current trawling regimes. This study collated data from 13 diverse regions of the globe spanning four continents. Within each region, we combined trawl intensity distributions and predicted abundance distributions of benthos groups with impact and recovery parameters for taxonomic classes in a risk assessment model to estimate benthos status. The exposure of 220 predicted benthos‐group distributions to trawling intensity (as swept area ratio) ranged between 0% and 210% (mean = 37%) of abundance. However, benthos status, an indicator of the depleted abundance under chronic trawling pressure as a proportion of untrawled state, ranged between 0.86 and 1 (mean = 0.99), with 78% of benthos groups > 0.95. Mean benthos status was lowest in regions of Europe and Africa, and for taxonomic classes Bivalvia and Gastropoda. Our results demonstrate that while spatial overlap studies can help infer general patterns of potential risk, actual risks cannot be evaluated without using an assessment model that incorporates trawl impact and recovery metrics. These quantitative outputs are essential for sustainability assessments, and together with reference points and thresholds, can help managers ensure use of the marine environment is sustainable under the ecosystem approach to management. 相似文献
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Richard Finkers Yuling Bai Petra van den Berg Ralph van Berloo Fien Meijer-Dekens Arjen ten Have Jan van Kan Pim Lindhout Adriaan W. van Heusden 《Euphytica》2008,159(1-2):83-92
Tomato (Solanum lycopersicum) is susceptible to gray mold (Botrytis cinerea). Quantitative resistance to B. cinerea was previously identified in a wild relative, S. neorickii G1.1601. The 122 F3 families derived from a cross between the susceptible S. lycopersicum cv. Moneymaker and the partially resistant S. neorickii G1.1601 were tested for susceptibility to B. cinerea using a stem bioassay. Three putative quantitative trait loci (pQTL) were detected: pQTL3 and pQTL9 reducing lesion growth
(LG) and pQTL4 reducing disease incidence (DI). For each pQTL, a putative homologous locus was identified recently in another
wild tomato relative, S. habrochaites LYC4. pQTL3 was confirmed by assessing disease resistance in BC3S1 and BC3S2 progenies of S. neorickii G1.1601. pQTL4 was not statistically confirmed but the presence of the S. neorickii resistance allele reduced DI in all three tested populations. The reduction in LG of pQTL9 was not confirmed but rather,
this locus conferred a reduced DI, similar to observations in the QTL study using S. habrochaites. The results are discussed in relation to other disease resistance loci identified in studies with other wild tomato relatives. 相似文献
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Laboratory toxicity tests are usually conducted under stable conditions, while exposures in the field occur under variable environmental conditions. Field studies are therefore more appropriate in understanding the effect of single or mixed pollutants in the environment. Short-term interactive effects of natural environmental factors (flooding and drought) and anthropogenic stressors (copper and salinity) on the earthworm Aporrectodea caliginosa were investigated using outdoor microcosm experiments. Specimens of the lumbricid earthworm A. caliginosa were exposed in microcosms loaded with soils with increasing salinity with electrical conductivity (EC) ranging from 0.08 to 1.05 dS m?1, with or without copper oxychloride spray treatments at recommended and elevated dosages. The experiment was conducted in August/September (end of winter) and repeated in November/December (end of spring) in the Stellenbosch area, South Africa to attain exposure under variable environmental conditions. In the soil, changes in Cu concentrations along the gradient of salinity were monitored using three methods: CaCl2, DTPA and nitric acid extraction. Survival, weight change, and cocoon production of worms and body Cu concentrations were used as indices of interaction. None of the three extraction methods could reveal interactive effects between salinity and Cu in both seasons either because concentrations of Cu were too low or below detection limits. Copper, on its own, did not have a significant effect on the measured worm parameters during both seasons. During the winter experiment, flooding of microcosms significantly reduced the survival and affected the weight change of worms, and probably caused leaching of chemicals. Interaction between salinity and Cu had no significant effects on the measured worm parameters in winter while salinity only had a significant negative effect on weight change of worms. During spring, significant synergistic interaction between salinity and Cu occurred but only at 0.3 dS m?1 by day 14 of the exposure period. At this and higher levels, salinity had a significant individual effect on survival and weight change of worms at days 14 and 28. The results indicate that higher toxicity of salinity could be expected during the dry spring periods than during the wet winter periods which are typical for southern temperate zones. 相似文献
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Ane T Laugen Georg H Engelhard Rebecca Whitlock Robert Arlinghaus Dorothy J Dankel Erin S Dunlop Anne M Eikeset Katja Enberg Christian Jørgensen Shuichi Matsumura Sébastien Nusslé Davnah Urbach Loїc Baulier David S Boukal Bruno Ernande Fiona D Johnston Fabian Mollet Heidi Pardoe Nina O Therkildsen Silva Uusi‐Heikkilä Anssi Vainikka Mikko Heino Adriaan D Rijnsdorp Ulf Dieckmann 《Fish and Fisheries》2014,15(1):65-96
Managing fisheries resources to maintain healthy ecosystems is one of the main goals of the ecosystem approach to fisheries (EAF). While a number of international treaties call for the implementation of EAF, there are still gaps in the underlying methodology. One aspect that has received substantial scientific attention recently is fisheries‐induced evolution (FIE). Increasing evidence indicates that intensive fishing has the potential to exert strong directional selection on life‐history traits, behaviour, physiology, and morphology of exploited fish. Of particular concern is that reversing evolutionary responses to fishing can be much more difficult than reversing demographic or phenotypically plastic responses. Furthermore, like climate change, multiple agents cause FIE, with effects accumulating over time. Consequently, FIE may alter the utility derived from fish stocks, which in turn can modify the monetary value living aquatic resources provide to society. Quantifying and predicting the evolutionary effects of fishing is therefore important for both ecological and economic reasons. An important reason this is not happening is the lack of an appropriate assessment framework. We therefore describe the evolutionary impact assessment (EvoIA) as a structured approach for assessing the evolutionary consequences of fishing and evaluating the predicted evolutionary outcomes of alternative management options. EvoIA can contribute to EAF by clarifying how evolution may alter stock properties and ecological relations, support the precautionary approach to fisheries management by addressing a previously overlooked source of uncertainty and risk, and thus contribute to sustainable fisheries. 相似文献