Differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies

Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues.     

新疆高等寄生植物(二)——列当科

本文研究了新疆列当科2属23种植物,用检索表列出了它们的不同,总结了前人的工作,并对它们的分布、生境、寄主给予了描述。     

多组离合器参与的换挡过程混合建模与仿真

在分析多组离合器参与的自动变速器换挡过程特点的基础上,指出该换挡过程是由连续系统与离散系统组成的混合系统,进而提出基于键合图理论的连续系统和基于有限状态机的离散系统的换挡过程混合建模方法。在Matlab／Simulink和Matlab／Stateflow平台上建立了包含多种状态的东方红1302R型橡胶履带拖拉机液压机械无级变速器的换挡过程仿真模型,仿真结果表明该建模方法能很好地模拟自动变速器换挡过程中离合器的动态行为,并通过仿真分析了其对换挡品质的影响。     

考虑陀螺效应的动力总成悬置系统分析和优化

应用陀螺力学理论,对传统动力总成悬置系统的无阻尼动力学方程进行修正,引入陀螺矩阵,得到新的动力学方程。对新的动力总成悬置系统动力学模型进行计算分析,研究了陀螺矩阵对频域、时域响应的影响。使用遗传算法分别对修改前后的方程进行优化计算,并进行比较分析。结果表明,对于低转速发动机,曲轴转动的陀螺效应不明显,而对于高转速的发动机,陀螺效应不能忽略,应以考虑陀螺力矩的方程为基础进行设计分析。     

基于中断控制的CVT电控系统数据采集系统

基于Infineorl SAK C164CI单片机,采用模块化方法,设计开发了CVT电控系统数据采集系统,包括中断控制的转速采集模块、10位精度A／D转换模块和串口通信模块,并对系统进行了台架和实车试验。试验结果证明,系统采集精度高、速度快、可靠性好、调整方便。     

涡流室柴油机燃烧过程模拟

利用FLUENT软件构建了描述S195型涡流室单缸柴油机燃烧过程的三维数学物理模型,并对气体在涡流室内的运动情况进行了数值模拟分析,获得了缸内气体的压力变化、温度变化以及碳烟排放的情况。实测结果表明,该模型能够比较准确地模拟涡流室柴油机的燃烧过程。     

小麦秸秆还田机粉碎装置性能影响因素试验与优化

利用设计的秸秆还田机粉碎装置进行了粉碎试验。以壳体包角、喂入速度、动定刀间隙为试验因素,秸秆的粉碎质量、粉碎功耗为试验指标,建立了粉碎质量和粉碎功耗的数学模型。随着喂入速度、壳体包角的增加和动定刀间隙的减小,粉碎功耗增大,粉碎质量提高。利用回归方程和主目标法,进行了参数的优化设计,结果为:粉碎室包角为70°、喂入速度为46.5m/s、动定刀间隙为10mm。     

生物炭对盐碱条件下海滨锦葵根系与土壤特性的影响

为探索生物炭对盐碱条件下植物根系与土壤特性的影响,以海滨锦葵为材料,在盐碱土壤中添加不同含量的生物炭,测定植株根系活力、根系生长、土壤pH值、土壤电导率、土壤容重、土壤孔隙度等指标。结果显示:在盐碱胁迫下,添加生物炭提高了海滨锦葵根系活力,中高浓度生物炭明显促进植株根系生长,土壤pH值与电导率均降低,土壤容重呈上升趋势,而土壤孔隙度呈现下降趋势。经比较,5.0%和7.5%生物炭对盐碱条件下海滨锦葵根系发育与土壤特性的调控效果相对较好。     

用PCR检测副溶血性弧菌耐热溶血素基因

根据副溶血性弧菌的耐热溶血素基因（ｔｄｈ）设计了２对引物，经聚合酶链式反应（ＰＣＲ）检测，所有１８株神奈川试验阳性副溶血性弧菌均呈阳性反应，而６株神奈川试验阴性副溶血性弧菌呈阴性反应，其他９种（３４株）弧菌和８株其他革兰氏阴性菌也呈阴性反应。检测敏感性可达２ＣＦＵ的副溶血性弧菌。     

Anatid herpesvirus 1 CH virulent strain induces syncytium and apoptosis in duck embryo fibroblast cultures

Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis.