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排序方式: 共有52条查询结果,搜索用时 15 毫秒
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Mada Triandala Sibero Yasuhiro Igarashi Ocky Karna Radjasa Agus Sabdono Agus Trianto Dewi Seswita Zilda Yusuf Jati Wijaya 《International Aquatic Research》2019,11(2):173-186
There is no report of diversity, biological properties and bioactive compounds of sponge-associated fungi from Indonesia’s mangrove ecosystem to date. This study was designed to isolate sponge-associated fungi from a mangrove ecosystem in Mangkang, to screen the antimicrobial and extracellular enzyme properties of the isolates, characterize the biologically promising isolates using molecular approaches, and profile the secondary metabolites using phytochemical and thin-layer chromatography (TLC) analyses. An unidentified sponge that lived in association with mangrove roots was collected from Mangkang. Total of eight associated fungi were isolated from the sponge. Among all isolates, only two fungi SPMKF 1 and SPMKF 6 produced extracellular amylase, another two fungi SPMKF 4 and 5 showed antibacterial activity against MRSA, and only one fungus SPMKF 8 was able to produce extracellular amylase and show antimicrobial activity against ESBL E. coli, Salmonella enterica ser. Typhi strain MDR and C. albicans, while SPMKF 2, SPMKF 3 and SPMKF 9 did not show any biological properties. The result of genetic characterization proved that SPMKF 1 was Cladosporium tenuissimum, SPMKF 4 was Eutypella sp., SPMKF 5 was Lasiodiplodia theobromae, SPMKF 6 was Fusarium keratoplasticum and SPMKF 8 was F. solani. Furthermore, an amylase gene was detected in fungi SPMKF 1, 6 and 8 while among the BGC, only NRPS genes were detected in SPMKF 4, 5 and 8. Interestingly, several same metabolites indicating the same retention factor (Rf) values in TLC were detected in the fungal crude extracts by TLC. 相似文献
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The male sterility system in hybrid seed production can eliminate the cost of emasculation and ensure seed hybridity through
avoidance of self pollination. GMS and CMS are two types of male sterility system that currently employed in pepper breeding.
Conversion from GMS to CMS will increase the male sterility proportion of female parent from 50 to 100%. In this study, segregation
analysis of four male sterile mutants consisting of one CMS mutant (CA1) and three GMS mutants (GA1, GA3 and GA4) showed that
each had single recessive gene inheritance. A modified complementation test was performed by replacing male sterile mutants
with their maintainer line as male parent. The nuclear restorer gene for CMS was independent of all nuclear restorer genes
for GMS and all nuclear restorer genes for GMS were independent each other. Further observation on CMS and GMS male sterility
loci revealed that GA1 and GA3 had mutated in both nuclear restorer genes for CMS and GMS, while CA1 and GA4 each carried
mutation in single male sterility system of nuclear restorer gene for CMS and GMS, respectively. Conversion from GMS to CMS
in the case of lines carried mutations in both sterility systems required only S-type cytoplasm donor, while lines carried
mutation in single nuclear restorer gene for GMS required not only S-type cytoplasm but also rf allele donors. The important finding is the broader function of maintainer line in certain male sterility system that can
be used as a maintainer or restorer line for other male sterility systems. We also confirmed that line CC1 is the general
restorer for both CMS and GMS systems. 相似文献
5.
Hamad H. Issa Junichi Tanaka Rachmaniar Rachmat Andi Setiawan Agus Trianto Tatsuo Higa 《Marine drugs》2005,3(3):78-83
Two new tricyclic alkaloids, polycitorols A (1) and B (2) have been isolated along with the known lepadiformine (3) from a marine ascidian of the family Polycitoridae. The structures of the new compounds were elucidated by analysis of NMR data and comparison with those of 3 and other related compounds [1–5]. Compounds 1 and 2 are closely related to cylindricines A and B, lacking C-4 oxygenation found in cylindricines and having a butyl instead of a hexyl appendage. NOE experiments on compounds 1 and 2 suggested the A/B ring fusion to be cis. 相似文献
6.
Enos Tangke Arung Irawan Wijaya Kusuma Yetti Mulyati Iskandar Seiji Yasutake Kuniyoshi Shimizu Ryuichiro Kondo 《Journal of Wood Science》2005,51(5):520-525
In our efforts to find new tyrosinase inhibitory materials, we investigated 44 Indonesian plants belonging to 24 families
for tyrosinase inhibitory activity. The extracts of 5 Artocarpus woods showed potent tyrosinase inhibitory activity (over 80% at 100 μg/ml) similar to a positive control, kojic acid. In Artocarpus woods, the extracts of the sapwoods showed stronger inhibitory activity than those of the heartwoods. Chlorophorin was isolated
as one of the active compounds in the sapwood of Artocarpus heterophyllus. The content of chlorophorin in sapwood was higher than that in heartwood.
Part of this paper was presented at the 53rd (Fukuoka, March 2003) and 54th (Hokkaido, August 2004) Annual Meetings of the
Japan Wood Research Society, and the 5th International Wood Science Symposium (Kyoto, September 2004) 相似文献
7.
Wulansari R Wijaya A Ano H Horii Y Makimura S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(5):579-584
This study was carried out to clarify the role of lymphocyte subpopulations and Babesia-specific antibody on the treatment of clindamycin in dogs infected with B. gibsoni. Ten beagle dogs were divided into two groups: an untreated group (5 dogs) and a clindamycin-treated group (5 dogs), which was administered clindamycin at 25 mg/ kg body weight, per os, q 12 hr from 7 days to 21 days post-infection (PI). On the acute stage of infection, clindamycin treatment resolved anaemia and other clinical findings. There were no significant differences between treated and untreated dogs either in parasitemia levels or Babesial IgG antibody levels. However, morphological changes that indicated degeneration in the majority of parasites were observed. The numbers of CD4(+) showed a significant increase in treated dogs, especially after treatment. On the chronic stage, CD4(+) cells maintained high level both of the treated and untreated dogs. Although parasitemia maintained low level, their relapses were occurred on the 49th day PI in treated dogs and on the 42nd and 63rd PI in untreated dogs. A rapid humoral antibody response was observed in treated dogs, however, lower humoral antibody responses in untreated dogs after relapses. The antibody levels of treated dogs were significantly higher than those of untreated dogs. These results suggested that clindamycin might not eliminate rapidly parasites from peripheral blood, but damage parasites, which might stimulate efficiently humoral and cellular immunity against Babesia infection, and result in an improvement of clinical conditions. 相似文献
8.
针对菊花人工采摘效率低、尚未实现机械化等问题,设计了一种气动翻转梳齿式菊花采摘装置。该采摘装置主要由采摘部件、清齿部件、气动抛送机构、丝杠升降机构、行走装置和收集装置等组成,利用梳齿的梳刷作用将花朵采摘下来,借助清齿部件和气动抛送机构完成收集工作,采摘部件的工作高度通过丝杠升降机构进行调节。根据菊花的生长特性和采摘要求,确定了采摘部件中偏置曲柄滑块机构和采摘梳齿的结构参数和运动参数。搭建了采摘样机,以曲柄转速、梳齿间距、机器行驶速度为试验因素,以采摘率、损伤率和含杂率为试验指标,进行了三元二次回归组合试验,建立了因素与指标间数学模型并确定了最优的参数组合,试验表明:在曲柄转速为47.94r/min、梳齿间距为8mm、机器行驶速度为0.17m/s的因素水平组合下,采摘效果最佳。此时,采摘率为92%,损伤率为1.83%,含杂率为10%。该气动翻转梳齿式菊花采摘装置运行稳定,通过性良好,满足菊花采摘的农艺要求。 相似文献
9.
Wijaya A Wulansari R Ano H Inokuma H Makimura S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(8):835-839
In order to identify the alternative effective chemotherapeutic agents for murine babesiosis, some selected drugs were examined for their efficacy against protozoan infection in the mouse-Babesia rodhaini (B. rodhaini) model. Clindamycin was not completely effective for elimination of parasites in a dose of 50 mg or 100 mg/kg BW/day b.i.d. but effective to prolong the life span of hosts, while it completely cured B. rodhaini infections in a dose of 200 mg. On the other hand, a double therapy consisting of 2 treatments with 100 mg clindamycin and 100 mg clindamycin and with 100 mg clindamycin and 100 mg tetracycline; respectively, and a single therapy with 100 mg tetracycline or 200 mg clindamycin, had a possibility to clear away B. rodhaini organisms from hosts. However, almost all the treatment groups, had a relapse of the infection within 10 days post treatment or re-treatment. Cured mice by treatment with clindamycin and clindamycin, or clindamycin and tetracycline showed complete resistance against challenge with B. rodhaini, while mice cured by administration of clindamycin at 200 mg or tetracycline at 100 mg showed incomplete resistance to challenge infection. The present data suggest that the two former chemotherapies can induce effective protective immunity (premunization), but the latter two chemotherapies induce incomplete premunization. 相似文献
10.
Wijaya CH Ulrich D Lestari R Schippel K Ebert G 《Journal of agricultural and food chemistry》2005,53(5):1637-1641
Three cultivars of snake fruits, Pondoh Hitam, Pondoh Super, and Gading, were freshly extracted using liquid-liquid extraction. The aroma compounds of the three samples were analyzed by GC-MS and GC-olfactometry using the nasal impact frequency (NIF) method. A total of 24 odor-active compounds were associated with the aroma of snake fruit. Methyl 3-methylpentanoate was regarded as the character impact odorant of typical snake fruit aroma. 2-Methylbutanoic acid, 3-methylpentanoic acid, and an unknown odorant with very high intensity were found to be responsible for the snake fruit's sweaty odor. Other odorants including methyl 3-methyl-2-butenoate (overripe fruity, ethereal), methyl 3-methyl-2-pentenoate (ethereal, strong green, woody), and 2,5-dimethyl-4-hydroxy-3[2]-furanone (caramel, sweet, cotton candy-like) contribute to the overall aroma of snake fruit. Methyl dihydrojasmonate and isoeugenol, which also have odor impact, were identified for the first time as snake fruit volatiles. The main differences between the aroma of Pondoh and Gading cultivars could be attributed to the olfactory attributes (metallic, chemical, rubbery, strong green, and woody), which were perceived by most of the panelists in the Pondoh samples but were not detected in the Gading samples. This work is a prerequisite for effective selection of salak genotypes with optimal aroma profiles for high consumer acceptance. 相似文献