果园生草的好处及种植技术

果园生草技术已成为发达国家开发成功的一项现代化、标准化的果园土壤管理技术,在我国,中国绿色食品发展中心已于1988年,将果园生草纳入绿色食品果业生产体系。     

不同光照处理下外施营养液和激素对万圣节法师叶色变化的影响[1]

To study the effects of light, external nutrition, and hormones on the color changes of Aeonium aeonium "Halloween" leaves. In this study, the 1-year-old stem cuttings of the Aeonium aeonium "Halloween" were used as materials. The leaves were sprayed with 25mg/L methyl jasmonic acid, 3‰ potassium dihydrogen phosphate, and 1‰ calcium magnesium boron mixture for two consecutive times, with an interval of 3 days. The experiment consisted of CK (water control), LS, DS (dark culture for 1 week). After 1 week of treatment, the leaf color parameters and anthocyanin pigment content were measured every 7 days for 6 consecutive weeks. The results showed that the treatment time and method had a significant impact on the leaf color parameters. In terms of brightness value L, as the treatment time extended, all three treatments showed an upward downward upward trend. Among them, the highest value was reached after 2 weeks of treatment, gradually decreased after 3 weeks, and sharply increased again after 6 weeks; In terms of red green saturation a value, after 2-4 weeks of treatment, both LS and DS treatments were significantly higher than CK, and the effect significantly decreased after 5 weeks; Light has a significant impact on a value, with DS treatment showing a significant decrease in a value after one week of shading, and a sharp increase after re lighting; In terms of the b value of yellow and blue saturation, the change in CK value is relatively small with the extension of treatment time, while the fluctuation in LS and DS treatment is significant; In terms of color saturation c value, DS treatment significantly increased with the prolongation of treatment time, and there was no significant change in LS and CK treatment within 1-3 weeks. After 3 weeks of treatment, there was no significant difference between the three treatments; With the extension of treatment time, the anthocyanin content of each treatment showed a trend of first increasing and then decreasing. After 2-3 weeks of treatment, the anthocyanin content was higher, and gradually decreased after 4 weeks of treatment; Correlation analysis shows that anthocyanin content is highly significantly positively correlated with a and c values, and anthocyanin content is a key factor in the red color of Aeonium aeonium "Halloween".     

绵羊副结核病定性诊断

通过流行病调查、皮内变态反应试验、补体结合试验、琼脂免疫扩散试验、病原检查及病理检查等综合检验,首次在省內某羊场作出绵羊副结核病的定性诊断。相隔32周,对该羊场同一发病群进行了两次检疫,第一次在1998头绵羊中,检出皮试阳性羊188头;血清学抽检432头,其中补反阳性19头、琼扩阳性45头。第二次在1606头绵羊中,检出皮试阳性羊32头;血清学抽检288头,其中补反阳性1头,琼扩阳性5头。     

京、津、冀区域扬沙天气气候变化特征分析

利用京、津、冀区域内所辖 6 5个气象观测站 1 95 4 - 2 0 0 2年的观测资料 ,分析了扬沙天气的气候变化特征。结果表明 :京、津、冀区域绝大部分地区是扬沙天气的主要影响区。高原、平原和丘陵地区是扬沙天气的多发区和易发区 ,宁晋、邢台一带是扬沙天气的高频区。扬沙的发生时间具有明显的季节变化特征 ;此区域内的扬沙呈现整体减少趋势 ,各分区域的变化趋势具有相似性 ,只是在减少幅度和年代际减少趋势上略有不同     

山羊MMP-9基因cDNA的克隆及生物信息学分析

为了克隆山羊基质金属蛋白酶-9(MMP-9)基因的cDNA片段,分析其基因序列,试验于无菌条件下采集泌乳期奶山羊乳腺组织并提取总RNA。根据已知其他物种的MMP-9基因保守性区域设计特异性引物,采用RT-PCR方法扩增MMP-9基因的CDS区,测序后对核苷酸和推导的氨基酸序列进行分析,并对8个物种进行聚类分析。结果表明:得到长度为2 245 bp的MMP-9基因cD-NA片段(GenBank登录号为JQ670877),其中包含2 130 bp的CDS全长;核酸序列分析结果显示,该基因编码709个氨基酸,与人、小鼠和牛的核苷酸序列进行比对,相似性分别为84%、80%、96%,氨基酸相似性分别为79%、73%、94%。     

鸡空肠弯曲菌感染的诊断

弯曲菌感染是由弯曲菌属(Campylobacter)空肠弯曲菌(C.jejuni)所引起的人畜共患肠道传染病。鸡空肠弯曲菌感染主要表现为肠炎、肝肿大。Hofstad等曾于     

甲砜霉素羟丙基-β-环糊精包合物测定方法和制备工艺

采取正交试验以包合物收得率、含药量和包合率为指标筛选最佳工艺,并用紫外分光光度法测定含量,优选羟丙基-β-环糊精包合甲砜霉素的最佳工艺.确定最佳条件为:包合温度为50 ℃,包合时间为1 h,羟丙基-β-环糊精与甲砜霉素摩尔比为1∶ 1.     

饥饿疗法对温和性禽流感的治疗

近年来蛋鸡温和型禽流感时有发生,产蛋鸡采食下降,产蛋率下降0%～40%不等。给养殖业带来重大经济损失,为了探讨饥饿疗法对温和性禽流感的治疗效果,笔者对发病鸡群采取口服抗流感纯中药及防继发感染西药饮水,停光照停料饥饿疗法。分别实验了发病初期饥饿疗法、发病中期饥饿疗法、发病后期饥饿疗法及常规方法治愈后     

高粱抗蚜基因的遗传分析和SSR标记定位

高粱蚜是高粱生产中的一种毁灭性害虫,利用植物的固有抗性是防治害虫的最有效途径。经多年杂交选育获得1个对高粱蚜免疫的高粱品种“河农16”,用其作亲本与感蚜品系“千三”进行杂交,对亲本、F1、F2抗蚜性鉴定表明,“河农16”和F1对蚜虫表现高抗,F2抗感分离符合3∶1,该抗蚜性受一对基因控制,表现出显性遗传。用已定位到连锁群上的微卫星标记和分离群体分析法,对抗蚜基因进行了连锁分析,发现1个与抗蚜基因连锁的微卫星标记(SSR标记),与抗蚜基因的遗传距离为8.7 cM。该标记位于第9连锁群上,因而将该基因初步定位于第9连锁群。     

双垂鹤鸵的人工孵化与育雏观察分析

双垂鹤鸵（又名食火鸡）在圈养条件下自然繁殖十分困难,石家庄市动物园通过技术攻关已成功掌握双垂鹤鸵的人工孵化繁殖技术。2019—2020年石家庄市动物园澳洲食草区一对食火鸡成功合笼配对,2020年后开始产卵。2021年石家庄市动物园首次采用人工孵化方式孵化出食火鸡幼雏,并且成功人工育雏。文中主要总结了2021和2022年成功人工孵化和人工育雏的3只食火鸡的一些经验,通过控制体重监测调整饲料喂量,引导幼雏学习采食以及做好动物观察及时调整饲养管理措施等,通过这种方式来提高双垂鹤鸵的孵化率和成活率,丰富动物种群,并加强对雏鸟常见疾病预防预警工作。