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排序方式: 共有156条查询结果,搜索用时 15 毫秒
1.
C. Jacyn Baker Daniel P. Roberts Norton M. Mock Bruce D. Whitaker Kenneth L. Deahl Andrey A. Averyanov 《Physiological and Molecular Plant Pathology》2005,67(6):1322
The plant apoplast is an important mediator of communication between the cell cytoplasm and its surroundings. Plant cell suspensions offer a convenient model system to gain insight into apoplastic physiology. Here, we describe a novel phenomenon that took place when two naturally occurring phenolics were added together to either soybean or tobacco cell suspensions. Acetosyringone (AS) and/or hydroxyacetophenone (HAP), phenolics found in the extracellular/apoplast of tobacco cells, were added to soybean or tobacco cell suspensions undergoing an oxidative burst. Individually, AS appeared to be utilized as a typical peroxidase substrate to scavenge hydrogen peroxide, while HAP was utilized at a much lower rate. However, when added together the rate of utilization of both phenolics increased and surprisingly resulted in the production of hydrogen peroxide. We have further characterized this novel phenomenon in suspension cells. This study demonstrates that certain phenolics in plants can cause co-oxidation which, as in animals, could alter the structure and bioactivity of surrounding phenolics. 相似文献
2.
Henningsen AD Murru FL Rasmussen LE Whitaker BR Violetta GC 《Fish physiology and biochemistry》2008,34(4):437-446
Levels of reproductively-related steroids were determined in captive male sand tiger sharks, Carcharias
taurus, maintained at two institutions: SeaWorld Adventure Park Orlando and the National Aquarium in Baltimore. Sexual conflicts
were absent at the former, but were documented at the latter. Serum titers of 17β-estradiol, progesterone, testosterone, and
5α-dihydrotestosterone were determined via radioimmunoassay in adult male sharks from 1988 to 2000. Sampling overlap between
the two institutions occurred for 3 months of the year, but steroid concentrations were compared only for April due to the
occurrence of sexual conflicts in the sharks at the National Aquarium in Baltimore in that month. For April, testosterone
and dihydrotestosterone were significantly higher in the SeaWorld males, and progesterone was significantly higher in the
National Aquarium in Baltimore males, while estradiol was not significantly different. Steroid levels were also determined
from serial samples taken monthly over 17 months from three male sharks and one female shark at the National Aquarium in Baltimore
in 2001–2002 and were compared with corresponding observed sexual conflicts. The steroid levels obtained showed distinct annual
hormonal cycles in the male sharks and corroborated a biennial cycle for the single serially-sampled female shark. Furthermore,
the steroid levels for individual males correlated with sexual conflicts as well as their position within the male dominance
hierarchy. As this species is depleted in some regions globally, insight into the steroid profile of mature sand tiger sharks
is important for a greater understanding of the relationship between their reproductive physiology and behavior, and may aid
in captive management and reproduction.
L. E. L. Rasmussen—Deceased. 相似文献
3.
M M Garner S D Atkinson S L Hallett J L Bartholomew R W Nordhausen H Reed L Adams B Whitaker 《Journal of fish diseases》2008,31(1):27-35
Renal myxozoanosis was diagnosed histologically in 11 captive, wild caught, adult weedy (common) sea dragons, Phyllopteryx taeniolatus, from three separate public aquaria in the United States. Myxozoan spores were visible in wet mounts of kidney tissue and were associated with renal tubular dilatation and tubular epithelial cell hypertrophy. Light and electron microscopy revealed spore morphology consistent with the genus Sinuolinea. Spores were spheroidal, slightly dorso-ventrally compressed, length (L) 17.1 x width (W) 16.4 x thickness (T) 15.6 microm, with two shell valves joined at a distinct, sinuous sutural ridge, and had two nearly spherical polar capsules, L 5.5 x W 5.0 microm, with five to seven turns of the polar filament. There were no extra-valvular ridges or protrusions. DNA sequencing required the design of three new primers that yielded 1740 bp of 18S ribosomal DNA sequence. The parasite was determined to be novel based on morphological and molecular data, and was given the name Sinuolinea phyllopteryxa after its vertebrate host. 相似文献
4.
Summary All accessions tested of four cultivated species ofCucurbita responded to seedling treatment with 250 ppm 2-chloroethylphosphonic acid by developing only pistillate flowers for an extended period. This growth regulator is a promising means of facilitating hybrid seed production of squash. A similar treatment did not induce flowering of wild species ofCucurbita that were vegetative under the environmental conditions of the experiment.Approved by the Director of the New York State Agricultural Experiment Station for publication as Journal Paper No. 1737. 相似文献
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Data derived from 340 dairy herds, mainly in southern England, between April 1998 and March 1999, showed that the average total culling rate was 22.1 per cent, with 5.6 per cent for infertility, 3.6 per cent for mastitis, 1.7 per cent for lameness, 2.0 per cent for poor milk yield, 3.7 per cent for age and 5.5 per cent for miscellaneous reasons which included death. The average annual rate of assisted calvings was 8.7 per cent, of injury 0.9 per cent, digestive disease 1.3 per cent ketosis 0.4 per cent, hypomagnesaemia 0.7 per cent, hypocalcaemia 5.3 per cent, mastitis 36.6 per cent, and lameness 23.7 per cent. There was a significant association (P<0.001) between higher rates of mastitis in cows housed in straw yards as opposed to cubicles and also between higher rates of lameness in cows housed in cubicles as opposed to yards (P<0.015). However, there were farms with low rates of mastitis in cows kept in straw yards and low rates of lameness in cows kept in cubicles. Larger herds tended to have more problems with lameness and higher bulk milk somatic cell counts (BMSCC). There was a positive association between BMSCC and mastitis rate. 相似文献
8.
采用世界先进的切向流超滤浓缩技术,对禽流感病毒(H9N2亚型)超滤浓缩成倍体积,测定血凝价HA和半数感染量EID50。结果HA成倍增加,废液HA呈阴性,病毒回收率达100%;受宿主鸡胚系统的影响,EID50与病毒量之间不是简单的直线关系,但病毒的活性不受影响。结果表明,所用超滤系统适用于禽流感病毒抗原的浓缩。 相似文献
9.
Duarte-Vázquez MA García-Padilla S García-Almendárez BE Whitaker JR Regalado C 《Journal of agricultural and food chemistry》2007,55(25):10396-10404
A peroxidase isozyme (BP) was purified to homogeneity from broccoli stems ( Brassica oleraceae var. maraton) discarded from industrial processing wastes. BP specific activity was 1216 ABTS [2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)] units/mg, representing 466-fold that of crude extract. BP is a monomeric glycoprotein containing 16% carbohydrates, with a molecular mass of 49 kDa and an isoelectric point close to 4.2. From kinetic data it showed a two-substrate ping-pong mechanism, and the catalytic efficiency measured as the rate-limiting step of free BP regeneration was 3.4 x 10(6) M(-1) s(-1). The ABTS K m value was 0.2 mM, which was about 20 times lower than that reported for acidic commercial horseradish peroxidase (HRP). Assessment of BP secondary structure showed 30% helical character, similar to HRP and cytochrome c peroxidase. BP lost only 25% activity after 10 min of heating at 55 degrees C and pH 6; it was stable in the pH range from 4 to 9 and showed an optimum pH of 4.6 using ABTS as substrate. BP was active on substrates normally involved in lignin biosynthesis, such as caffeic and ferulic acids, and also displayed good catechol oxidation activity in the presence of hydrogen peroxide. Reverse micellar extraction was successfully used as potential large-scale prepurification of broccoli peroxidase, achieving a purification factor of 7, with 60% activity yield. Stems from the broccoli processing industry have a high potential as an alternative for peroxidase purification. 相似文献
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