首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   578篇
  免费   44篇
林业   18篇
农学   26篇
基础科学   3篇
  74篇
综合类   122篇
农作物   27篇
水产渔业   37篇
畜牧兽医   276篇
园艺   7篇
植物保护   32篇
  2023年   8篇
  2022年   10篇
  2021年   13篇
  2020年   23篇
  2019年   14篇
  2018年   15篇
  2017年   12篇
  2016年   12篇
  2015年   17篇
  2014年   13篇
  2013年   20篇
  2012年   32篇
  2011年   35篇
  2010年   17篇
  2009年   14篇
  2008年   24篇
  2007年   31篇
  2006年   32篇
  2005年   33篇
  2004年   26篇
  2003年   25篇
  2002年   22篇
  2001年   7篇
  2000年   13篇
  1999年   4篇
  1998年   7篇
  1997年   4篇
  1996年   6篇
  1995年   10篇
  1994年   2篇
  1993年   6篇
  1992年   3篇
  1991年   5篇
  1990年   2篇
  1989年   13篇
  1988年   26篇
  1987年   6篇
  1986年   6篇
  1985年   4篇
  1983年   3篇
  1982年   2篇
  1981年   2篇
  1979年   5篇
  1977年   2篇
  1974年   3篇
  1972年   2篇
  1969年   2篇
  1968年   2篇
  1967年   6篇
  1953年   2篇
排序方式: 共有622条查询结果,搜索用时 15 毫秒
1.
The genetic similarity of Campylobacter jejuni isolates from pets, compared to human clinical cases and retail food isolates collected in Ireland over 2001-2006 was investigated by cluster analysis of pulsed-field gel electrophoresis (PFGE) fingerprinting profiles. Comparison of the PFGE profiles of 60 pet isolates and 109 human isolates revealed that seven (4.1%) profiles were grouped in clusters including at least one human and one pet C. jejuni isolate. In total six (1.6%) of 60 pet and 310 food profiles were in clusters with at least one food and one pet C. jejuni isolate. The detection of only a small number of genetically indistinguishable isolates by PFGE profile cluster analysis from pets and from humans with enteritis in this study suggests that pets are unlikely to be an important reservoir for human campylobacteriosis in Ireland. However, genetically indistinguishable isolates were detected and C. jejuni from pets may circulate and may contribute to clinical infections in humans. In addition, contaminated food fed to pets may be a potential source of Campylobacter infection in pets, which may subsequently pose a risk to humans.  相似文献   
2.
3.
BACKGROUND: Knowledge of breed associations is valuable to clinicians and researchers investigating diseases with a genetic basis. HYPOTHESIS: Among symptomatic dogs tested for exocrine pancreatic insufficiency (EPI) by canine trypsin-like immunoreactivity (cTLI) assay, EPI is common in certain breeds and rare in others. Some breeds may be overrepresented or underrepresented in the population of dogs with EPI. Pathogenesis of EPI may be different among breeds. ANIMALS: Client-owned dogs with clinical signs, tested for EPI by radioimmunoassay of serum cTLI, were used. METHODS: In this retrospective study, results of 13,069 cTLI assays were reviewed. RESULTS: An association with EPI was found in Chows, Cavalier King Charles Spaniels (CKCS), Rough-Coated Collies (RCC), and German Shepherd Dogs (GSD) (all P < .001). Chows (median, 16 months) were younger at diagnosis than CKCS (median, 72 months, P < .001), but not significantly different from GSD (median, 36 months, P = .10) or RCC (median, 36 months, P = .16). GSD (P < .001) and RCC (P = .015) were younger at diagnosis than CKCS. Boxers (P < .001), Golden Retrievers (P < .001), Labrador Retrievers (P < .001), Rottweilers (P = .022), and Weimaraners (P = .002) were underrepresented in the population with EPI. CONCLUSIONS AND CLINICAL IMPLICATIONS: An association with EPI in Chows has not previously been reported. In breeds with early-onset EPI, immune-mediated mechanisms are possible or the disease may be congenital. When EPI manifests later, as in CKCS, pathogenesis is likely different (eg, secondary to chronic pancreatitis). Underrepresentation of certain breeds among dogs with EPI has not previously been recognized and may imply the existence of breed-specific mechanisms that protect pancreatic tissue from injury.  相似文献   
4.
This article describes the management of otitis media in a domestic rabbit (Oryctolagus cuniculus) that presented with a history of chronic upper respiratory disease. Deep nasal culture yielded a pure growth of Bordetella bronchiseptica susceptible to chloramphenicol. To further evaluate recurring clinical signs after treatment with chloramphenicol, skull radiographs were obtained and showed an increased density in the right tympanic bulla. A ventral bulla osteotomy was performed, and the success of treatment was determined by the resolution of respiratory signs and the absence of increased radiographic density in the right tympanic bulla 5 months postoperatively.  相似文献   
5.
Exposure of livestock grazing to forage productivity variation and to market fluctuations affects the risk of investment and returns from cow-calf operations, but little work has been done to empirically compare these returns to the returns that would be demanded by financial markets from assets with similar risk and return characteristics. This study uses historical forage production data from three rangeland locations in California, and cattle and hay prices, to simulate financial statements for three hypothetical cow-calf producers in the period 1988–2007. Return on investment from year to year incorporates the variability and risk associated with dependence on natural forage production. Performance is then compared to the actual performance of a diversified portfolio of assets using the Capital Asset Pricing Model, from which the theoretical cost of capital for these hypothetical grazing enterprises is derived. Much like other agricultural enterprises, cow-calf production in California has low market risk and a low theoretical cost of capital. This theoretical cost of capital is still greater than the historical return from livestock production (excluding land appreciation) in the western United States, adding further backing to the point often made in the literature that ranchers who engage in cow-calf production are receiving benefits beyond the commercial returns from livestock production alone.  相似文献   
6.
Thirty adult, client-owned dogs were diagnosed with hypothyroidism based on history, physical examination findings, hematologic and biochemical abnormalities, thyrotropin (TSH) response testing, endogenous canine thyrotropin (cTSH) concentration, or both, and total serum thryoxine concentration. All dogs received levothyroxine (L-thyroxine) at an initial dose of 22 μg/kg PO sid in either a tablet (13 dogs) or chewable form (17 dogs). Energy expenditure of each dog during apparent rest was estimated with an open-flow indirect calorimetry system by determining the rates of carbon dioxide production and oxygen consumption. Energy expenditure of apparent rest (EE) was lower in untreated hypothyroid dogs compared with reference values for EE. After treatment with L-thyroxine, EE of the hypothyroid dogs was significantly ( P < .05) higher than pretreatment values.  相似文献   
7.
To compare the genetic susceptibility of elk (Cervus elaphus nelsoni) with various alleles of the PRNP gene, which encodes the normal cellular prion protein, to chronic wasting disease (CWD), eight 8-month-old elk calves of 3 genotypes (2 132MM, 2 132LM, and 4 132LL) were orally dosed with CWD-infected brain material from elk. During postinoculation (PI) month 23, both 132MM elk had lost appetite, developed clinical signs of weight loss and central nervous system (CNS) dysfunction, and were euthanized. Two other elk (both 132LM) developed similar clinical signs of disease and were euthanized during PI month 40. All 4 affected elk had microscopic lesions of spongiform encephalopathy (SE), and PrPres, the disease-associated form of the prion protein, was detected in their CNS and lymphoid tissues by use of immunohistochemical (IHC) and Western blot (WB) techniques. These findings indicate that elk with MM and LM at codon 132 are susceptible to orally inoculated CWD. All 4 LL elk are alive at PI year 4 and are clinically normal, which suggests that 132LL elk may have reduced susceptibility to oral infection with CWD-infected material or may have prolonged incubation time.  相似文献   
8.
Francisella tularensis type A is the primary cause of tularemia in animals and humans in North America. The majority of research on F. tularensis has been done with the attenuated live vaccine strain (LVS), which is a type B, but very few wild-type F. tularensis strains have been characterized. A gram-negative coccobacillus that was isolated in pure culture from the lungs of a cat that died after being lost for 5 days was received for identification at the Virginia-Maryland Regional College of Veterinary Medicine Teaching hospital. The isolate (strain TI0902) was not identified (or was misidentified) by commercial identification systems; however, it was identified as F. tularensis subspecies tularensis (type A) by sequencing a portion of the 16S ribosomal RNA gene. Furthermore, repetitive extragenic palindromic sequences-polymerase chain reaction amplified a 4-kb DNA fragment from TI0902 that was characteristic of F. tularensis type A but not type B. The electrophoretic profile of the lipopolysaccharide of strain TI0902 was identical to that of the LVS by Western blotting with antiserum to LVS. The protein-enriched outer membrane of strain TI0902 contained 6-8 proteins, which were similar in molecular size to those from the LVS. Electron microscopy of negatively stained and alcian blue-stained LVS and TI0902 cells showed that both strains were coccobacillary in shape and may be encapsulated. However, after mouse challenge, the TI0902 strain was clearly more virulent than the LVS strain. Results of this study indicate that the genotype and phenotype of wild-type F. tularensis type A strain TI0902 is similar, but not identical, to that of the LVS strain. Further studies will help determine whether pathogenesis and host-pathogen interactions are also similar between the 2 strains.  相似文献   
9.
Oral lufenuron is reportedly an effective treatment for some cats with dermatophytosis. The purpose of this study was to determine if lufenuron, when used as a pre-treatment prior to challenge exposure, would be protective against the development of infection after the direct topical application of fungal macrocondia (Microsporum canis spores). Three groups (n = 6/group) of juvenile cats were treated with either monthly oral lufenuron (30 or 133 mg/kg) or placebo. After 2 months of treatment, kittens were challenged using 10(5)Microsporum canis spores applied to the skin under occlusion. Cats were examined weekly and the following data collected: Wood's lamp examination; scoring for scale/crust, erythema and induration; lesion size; and the development of satellite lesions. Fungal cultures were performed bi-weekly. All cats became infected; the infections progressed, and then regressed, in a similar fashion in all groups. There were no consistent statistically significant differences in weekly infection scores between treated and untreated cats throughout the study. Treated cats did not recover faster than untreated cats. We conclude that oral lufenuron at the dosing schedule and conditions used in this study did not prevent dermatophytosis or alter the course of infection by direct topical challenge.  相似文献   
10.
Abstract

This review paper provides an overview of the role of breeding objectives in livestock industries. The analyst developing a breeding objective must use the many and varied tools available in an appropriate theoretical context to quantify the benefits of alternative trait changes. The development in tools for deriving economic values has been from simple profit equations to detailed bio-economic models simulating the whole production system or industry. The importance of including the perspective of the stakeholders is also stressed. Stakeholder perspectives can be considered by using stated preference methods such as choice experiments. Also desired gain index or selection indices can be used in combination with profit equations or bio-economic models to adjust for undesirable genetic changes in breeding objective traits. This review shows that defining appropriate breeding objectives to account for the perspectives of different key stakeholders requires a multi-disciplinary approach.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号