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Induction of diplodiosis, a neuromycotoxicosis, in domestic ruminants with cultures of indigenous and exotic isolates of Diplodia maydis 总被引:1,自引:0,他引:1
T S Kellerman C J Rabie G C van der Westhuizen N P Kriek L Prozesky 《The Onderstepoort journal of veterinary research》1985,52(1):35-42
Diplodiosis, a neuromycotoxicosis, principally of cattle, which is characterized by ataxia, paresis and paralysis, was induced in 13 cattle, 16 sheep and 3 goats, by dosing them with Diplodia maydis [= D. zeae (Schw.) Lév.] cultured on sterilized maize seeds. The results of these experiments confirmed the findings of earlier workers that diplodiosis is a mycotoxicosis caused by D. maydis. The intoxication was induced with cultures of South African isolates of D. maydis obtained from local maize, one of which was associated with a suspected field outbreak, and with cultures of isolates from maize imported from the United States of America and Argentina. Other findings emerging from the experiments were, inter alia, that cultures incubated for less than 8 weeks were seemingly non-toxic, that there was little individual variation in response of cattle to cultures of the different toxic isolates or batches of the isolates, that apparent relapses of clinical signs can occur several weeks after dosing had ceased and that a small percentage of animals can show permanent locomotory disturbance. Light microscopical examination revealed no lesions in acutely affected animals, but an extensive laminar subcortical status spongiosis was evident in the cerebrum and cerebellum of a sheep that had been long paralysed and a steer that had permanent locomotory disturbance. 相似文献
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F Ardón A Döhring X Le Thi KF Weitze D Waberski 《Reproduction in domestic animals》2003,38(2):161-165
The goal of this study was to determine the ability of the Hanover gilt model to assess in vivo fertilizing capacity of preserved sperm and to consider whether any modifications to this model were needed. This model evaluates the fertilizing capacity of semen based on the fertilization rate, the rate of normal embryos and the accessory sperm count of 3–5‐day embryos. Its distinguishing characteristics are the use of one‐time insemination of sperm in reduced numbers, of spontaneously ovulating gilts and of ovulation detection through ultrasound examination of ovaries. Reduced sperm numbers allow for an accurate evaluation of the fertilizing potential of different semen treatments, thereby avoiding the compensatory effect of doses calibrated to maximize fertility. The model's usefulness was assessed in a trial run designed to compare the fertilizing capacity of liquid boar semen diluted into two different extenders. The diluent, the boar and the backflow, had no significant effect on any of the parameters studied. Gilts inseminated less than 24 h before ovulation had a significantly higher (p < 0.01) fertilization rate and accessory sperm cell count (p < 0.05) than those inseminated more than 24 h before ovulation. Very good/good embryos from homogeneous litters (only very good/good embryos were present) had a significantly higher (p < 0.01) accessory sperm count than those from heterogeneous litters (at least one embryo was of a different quality and/or oocytes were present). Both very good/good and degenerated/retarded embryos from heterogeneous litters had low accessory sperm numbers. This suggests that accessory sperm count is significantly related to the quality of the litter, but not to the quality of the embryo within gilts. It can be concluded that the Hanover gilt model is sensitive enough to show fertility differences (in this study, those associated with in vivo ageing of semen), while using relatively few gilts and little time. 相似文献
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P A Basson T S Kellerman P Albl L J Von Maltitz E S Miller W G Welman 《The Onderstepoort journal of veterinary research》1975,42(4):135-147
An outbreak of amaurosis and paresis was observed amongst sheep, and occasionally cattle, which had been grazing on pastures consisting mainly of Helichrysum argyrosphaerum DC. A similar syndrome was produced by feeding the suspect plant to sheep, thereby providing proof if its toxicity. Although none of the sheep became blind and only one developed paralysis, typical sponly lesions were detected in the brains and optic fasciculi of all the experimental animals. The lesion had specific predilection sites, such as the white matter around the lateral ventricles, the optic tracts plus chiasm, the pyramidal tracts and the brachium pontis. Enlargement of the optic fasciculi furthermore gave rise to malacia, papilloedema and retinal changes. Some aged sheep developed cataracts approximately 2-3 months after the initial outbreaks of amaurosis had occurred. Circumstantial and histopathological evidence suggests that the cataracts may be due to chronic Helichrysum poisoning, but this could not confirmed in the present investigation. 相似文献
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Leukoencephalomalacia in a horse induced by fumonisin B1 isolated from Fusarium moniliforme 总被引:17,自引:0,他引:17
W F Marasas T S Kellerman W C Gelderblom J A Coetzer P G Thiel J J van der Lugt 《The Onderstepoort journal of veterinary research》1988,55(4):197-203
Each of two horses was dosed by stomach tube with culture material on maize of Fusarium moniliforme MRC 826. One horse developed severe hepatosis and mild oedema of the brain after 6 doses of 2.5 g of culture material/kg body mass/day in 7 days. The second horse, in a similar experiment but at a dosage rate of 1.25 g/kg/day, developed mild hepatosis and moderate oedema of the brain. In both animals the brain oedema was particularly noticeable in the medulla oblongata. The mycotoxin fumonisin B1 was extracted and purified from the culture material of F. moniliforme MRC, 826 which contained approximately 1 g/kg of this compound. A horse was injected intravenously 7 times from Day 0-Day 9 with 0.125 mg of fumonisin B1/kg body mass/day. Clinical signs of neurotoxicosis, which appeared on Day 8, included nervousness followed by apathy, a wide-based stance, trembling, ataxia, reluctance to move, paresis of the lower lip and tongue, and an inability to eat or drink. Euthanasia was performed on the horse on Day 10 while the animal was in a tetanic convulsion. The principal lesions were severe oedema of the brain and early, bilaterally symmetrical, focal necrosis in the medulla oblongata. This report provides experimental evidence that fumonisin B1, produced by F. moniliforme, causes equine leukoencephalomalacia. 相似文献
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P W Nel R A Schultz P Jordaan L A Anderson T S Kellerman C Reid 《The Onderstepoort journal of veterinary research》1987,54(4):641-644
Urginea physodes (Jacq.) Bak., a species closely related to or possibly synonymous with U. pusilla, is described and its distribution given. Four bufadienolides were isolated from U. physodes and the approximated LD50 and cumulative effect of some of them determined in guinea pigs. The most toxic one proved to be mildly cumulative. Typical signs of acute cardiac glycoside poisoning, involving the locomotory, gastro-intestinal, respiratory and cardiac system, were seen in the field cases and/or were experimentally induced by the plant. Similar signs could also be induced by injecting the isolated bufadienolide, physodine A, to a sheep. 相似文献
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