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研究了利用玉米85个 DNA 限制性片段长度多态性(RFLP)标记在玉米自交系7922与5003杂交 F2群体中的分离。结果表明,8.33%的标记显著或极显著地偏离了预期的孟德尔比例而表现偏分离,发现了2个偏分离的染色体热点,即第1染色体上的 umc128-umc107和第3染色体上的 asg24-asg48,其偏分离形成的主要原因是存在配子体选择。 相似文献
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Robson RV Alves Tatiana Soares Elinaldo FL Bento Ricardo S Roldan‐Filho Brbara SS Souza Marcele KN Lima Jssica S Nascimento Luana CBB Coelho Roberto A S Thmarah A Lima Gabriel GA Gonalves Fbio A Brayner Luiz C Alves Daniela MAF Navarro Thiago H Napoleo Patrícia MG Paiva 《Pest management science》2020,76(2):730-736
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Resistance of Fasciola hepatica to triclabendazole 总被引:1,自引:0,他引:1
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Serum and Tissue Steroid Hormone Levels in Canine Mammary Tumours: Clinical and Prognostic Implications 下载免费PDF全文
FL Queiroga D Pérez‐Alenza A González‐Gil G Silván L Peña JC Illera 《Reproduction in domestic animals》2015,50(5):858-865
Hormonal dependency of canine mammary tumours (CMT) has been studied over the last few decades. However, studies assessing the prognostic and predictive potential of serum and/or tissue steroid hormone levels are still scarce in CMT. To the best of our knowledge, this is the first report relating serum and tissue levels of steroid hormones and prognosis in dogs. Serum and tumour tissue from 45 female dogs with spontaneous CMT were included in the study. Moreover, serum and normal mammary tissue from 13 healthy female dogs were also included as controls. Steroid hormones were determined by competitive enzyme immunoassay. Overall, levels of steroid hormones in serum and tissue homogenates were significantly different between malignant and benign mammary tumours (p < 0.01), except for progesterone (P4) serum levels that revealed no statistical differences between groups. In malignant tumours, oestrone sulphate (SO4E1), dehydroepiandrosterone (DHEA), androstenedione (A4), testosterone (T) and P4 elevated tissue concentrations were significantly associated with tumour relapse and/or distant metastasis during follow‐up. A significant association was found between elevated tissue SO4E1 (p = 0.003), 17β‐oestradiol (E2) (p = 0.036), DHEA (p = 0.022), A4 (p = 0.001) and P4 (p = 0.013) concentrations and shorter disease‐free survival and overall survival in female dogs with malignant mammary tumours. The high levels of tissue steroids found in cases of poor prognosis open the possibility of additional new therapeutic approaches. Future clinical trials will be needed to clarify the usefulness of targeting steroid hormones in the treatment of this neoplastic disease. 相似文献
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治疗奶牛乳腺炎的新药盐酸吡利霉素研究进展 总被引:1,自引:0,他引:1
综述了治疗奶牛乳腺炎的新药——盐酸吡利霉素最新研究进展.对其基本药学性质、药动、药效、毒性和残留等多方面进行了详细阐述. 相似文献
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P Rodriguez‐Villamil FL Ongaratto M Fernandez Taranco GA Bó 《Reproduction in domestic animals》2014,49(1):79-84
Three experiments were designed to test a solid‐surface vitrification system for bovine in vitro‐produced embryos and to develop a simple method of in‐straw dilution after warming, which can be potentially used for direct transfer in the field. Experiment 1 evaluated embryo survival rates (i.e. re‐expansion and hatching) after vitrification and warming in three different solutions: VS1 (20% ethylene glycol (EG) + 20% propanediol (PROH) + 0.25 m trehalose (Tr)), VS2 (20% EG + 1M Tr) or VS3 (30% EG + 0.75 m Tr). Re‐expansion and hatching rates were higher (p < 0.05) for embryos vitrified in VS3 (72.2 ± 1.9 and 58.2 ± 0.8) than VS1 (64.4 ± 0.9 and 37.2 ± 2.5) or VS2 (68.5 ± 1.5 and 49.6 ± 1.0; p < 0.05). Experiment 2 was designed to compare two methods of vitrification: glass micropipettes or solid surface, using the VS1 or VS3 solutions. No significant differences were detected between the two methods; but re‐expansion and hatching rates were higher (p < 0.05) with VS3 (73.5 ± 3.1 and 47.1 ± 2.1) than VS1 (63.3 ± 3.3 and 39.7 ± 2.8). In experiment 3, embryos were vitrified by solid surface in VS1 or VS3 solutions and cryoprotectants were diluted in‐straw after warming in a TCM 199, 0.25 m sucrose solution or holding media. Survival rates of embryos vitrified in VS3 did not differ between those exposed to 0.25 m sucrose (74.7 ± 1.3 and 57.2 ± 2.2) or holding (77.3 ± 1.4 and 58.0 ± 2.5) medium after warming; however, survival rates of embryos vitrified in VS1 were higher (p < 0.05) in those exposed to 0.25 m sucrose (67.7 ± 2.3 and 47.0 ± 1.7) than holding medium (54.5 ± 1.0 and 27.7 ± 3.1). In conclusion, solid‐surface vitrification using simplified EG‐based solutions and in‐straw dilution with holding media may be a practical alternative for cryopreservation and direct transfer of in vitro‐produced bovine embryos. 相似文献
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Collette BB Carpenter KE Polidoro BA Juan-Jordá MJ Boustany A Die DJ Elfes C Fox W Graves J Harrison LR McManus R Minte-Vera CV Nelson R Restrepo V Schratwieser J Sun CL Amorim A Brick Peres M Canales C Cardenas G Chang SK Chiang WC de Oliveira Leite N Harwell H Lessa R Fredou FL Oxenford HA Serra R Shao KT Sumaila R Wang SP Watson R Yáñez E 《Science (New York, N.Y.)》2011,333(6040):291-292
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