评《百年孤独》

马尔克斯的《百年孤独》是一部具有深刻社会历史内涵的文学作品。它通过对人物形象的塑造。揭示了“孤独”这一拉美民族心理，反映了作者的民族观。从《百年孤独》中，可以看到它对魔幻现实主义文学的继承和发展。     

Identification and molecular characterization of a novel flavivirus isolated from Pekin ducklings in China

A flavivirus-associated disease of egg-laying ducks was observed in eastern China in 2010, and a novel mosquito-borne flavivirus, Tembusu virus (TMUV), was isolated (Cao et al., 2011). Following up on the earlier study, a virus similar to TMUV was isolated recently from ducklings and characterized. We report that (1) the recently isolated virus, TMUV ZJ-6, replicated in vertebrate cells (DF-1, BHK-21) as well as in mosquito cells (C6/36) and caused cytopathic effect (CPE) in the cell lines tested; (2) extracellular viral particles examined by electron microscopy were approximately 45 nm in diameter and enveloped; (3) the full-length genome of the virus was determined, showing that the TMUV ZJ-6 is more closely related to the Ntaya group of viruses than other members of the Flaviviridae based on the data of phylogenetic analyses. Most importantly, the disease of ducklings was reproducible after administration of plaque-purified virus by intracerebral (i.c.), subcutaneous (s.c.) or intranasal (i.n.) inoculation. This is the first report that TMUV infects not only egg-laying ducks but also 3-21 days-old ducklings. The findings extend our understanding of how the virus spreads and causes disease.     

益生菌发酵饲料对猪的生长和肉质的影响

应用益生菌发酵饲料喂猪,能提高饲料中粗蛋白含量,明显降低猪粪臭味,促进生长、降低料肉比和提高猪肉品质。     

浅谈动物检疫电子出证

本文阐述了动物检疫电子出证的优点,强调了开展该项工作对规范动物检疫出证,提高检疫效率以及提升动物产品安全监管水平的重要意义,认真分析了电子出证在系统设计、便携使用出证不规范及设备不足等方面存在问题,建议通过完善电子出证系统和出证设备,加大投入,加强培训,以期进一步推进动物检疫电子出证工作深入开展。     

玉米价格高涨后的蛋鸡饲料解决方案

进入2006年以来,由于玉米价格持续走高,致使饲料生产成本提高。特别是近期小麦和玉米的倒挂价差越来越大,局部地区甚至达到160￣220元/t,使得部分饲料企业和养殖场开始在生产中用小麦替代玉米。当前,中原地区玉米质次价高,霉变严重,养殖户在当地要找到优质玉米很不容易,因此,多     

禽类熟肉制品软包装技术

禽类熟肉制品具有丰富的蛋白质,微生物十分容易繁殖,而且肉制品中的脂肪在贮存中易氧化变质,因此禽类熟肉制品软包装技术关键是能使产品延长保质期,保证食品安全和质量.……     

楹树枝枯病病原菌的研究

文章描述了楹树枝枯病的症状,经分离培养,初步鉴定病原菌为可可球二孢菌(Botryodiplodia theobromae Pat.),并对该病的病原及其发生规律等方面进行研究,提出木蠹蛾的发生与楹树的发病有一定的关系。     

苹果园环境监控系统的研究与设计

随着苹果产业的发展,苹果园的机械化、自动化已经成为制约我国苹果产业发展的主要因素.为此,针对苹果园环境,利用先进的计算机技术,以单片机为核心,研制一种能对苹果树生长环境进行科学监测、科学分析和有效控制的全方位自动控制系统已成为我国苹果产业发展的必然趋势.该系统是一种融采集和监测环境信息、有效控制与自动供电于一体的全面机械化及自动化的控制系统,具有采集范围广、监控数据精确以及操作简单等优点,尤其是其带有太阳能自动供电模块,既节能,又环保,适用于苹果园的大面积应用.     

集约化奶牛养殖场不同粪尿处理阶段氮素分布及氨排放特征

为促进奶牛养殖场的大气氨排放控制,形成奶牛养殖场粪便中氨排放的阻控体系,该文在冬季和夏季对内蒙古呼和浩特地区奶牛养殖场A和奶牛养殖场B的大气、牛粪和牛尿进行了采样试验分析,研究了2种奶牛养殖场不同处理工艺的氨排放特征。静态试验结果表明,奶牛养殖场A和奶牛养殖场B氨气排放浓度最高的是氧化塘处理工艺、预处理工艺,分别为冬季0.862,3.169 mg/m3,夏季2.785,2.130 mg/m3。动态试验结果表明,牛粪的氨排放系数要高于牛尿1.85倍,奶牛养殖场A和奶牛养殖场B平均排放系数分别为29.23%、49.36%。奶牛养殖场A和奶牛养殖场B总大气氨排放量分别为冬季172.69,1 101.00 kg/d,夏季284.70、1 395.32 kg/d。2种处理工艺冬季和夏季大气氨含量均满足畜禽场环境质量标准,但超过人居空气质量标准。     

Development and validation of an indirect competitive enzyme-linked immunosorbent assay for the screening of tylosin and tilmicosin in muscle, liver, milk, honey and eggs

Incorrect use of tylosin and tilmicosin could result in allergy and select resistance. To monitor the illegal use of these antibiotics in animals, a monoclonal-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been established. Several haptens were synthesized and conjugated to carrier protein. Female Balb/c mice were inoculated with the four different conjugates to produce monoclonal antibodies according to the schemes of immunization. Aftercell fusion and culture several times, nine hybridoma cell lines were isolated. Only one, 3C4 that has isotype IgG2a, was selected for detailed study. The cross-reactivity of the monoclonal antibody 3C4 to tylosin and tilmicosin was 100% and 51% respectively. The standard curves based on the tylosin and tilmicosin matrix calibration ranged from 2.5 to 40 μg L(-1), with an IC(50) value of 6.1 μg L(-1) and 12.1 μg L(-1), respectively. The limits of detection of the ic-ELISA ranged from 5.1 μg kg(-1) to 13.8 μg kg(-1) in edible animal tissues. The recoveries were 74.1% to 120.7% with less than 18.6% of the coefficient of variation when tylosin and tilmicosin were spiked in various biological matrices with the concentrations of 25.0-200.0 μg kg(-1). Good correlations between the results of the ic-ELISA and high performance liquid chromatography were observed in the incurred tissues. These results suggest that the ic-ELISA is a sensitive, accurate and low-cost method that would be a useful tool for the screening of the residues of tylosin and tilmicosin in muscle, liver, milk, honey and eggs.