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排序方式: 共有424条查询结果,搜索用时 15 毫秒
1.
Kamonpan Awaiwanont Wisnu Gunarso Munefumi Sameshima Seiichi Hayashi Gunzo Kawamura 《Fisheries Science》2001,67(5):804-810
ABSTRACT: Morphology of the photoreceptor cells and tapetum of the Japanese anchovy Engraulis japonicus was studied by histologically and by chemical analysis. The Japanese anchovy has a duplex retina. The cones form parallel rows consisting of alternately placed long cones and bifid cones. Both types of cones are intimately associated and form triple units that are regularly spaced along the row of cones. The rods are grouped and stacked. This fish has a retinal tapetum lucidum composed of guanine and hypoxanthine. Three structures of the tapetum lucidum were recognized: platelet, diamond and rod types. Photomechanical changes include movements of the photoreceptor cells and the retinal tapetum. The retina of the Japanese anchovy is thought to be highly sensitive and well adapted to a dim light environment. 相似文献
2.
Competitive enzyme-linked immunosorbent assay for the detection of the antibodies specific to akabane virus. 总被引:5,自引:0,他引:5
Tomoyuki Tsuda Kazuo Yoshida Tohru Yanase Seiichi Ohashi Makoto Yamakawa 《Journal of veterinary diagnostic investigation》2004,16(6):571-576
A competitive enzyme-linked immunosorbent assay (C-ELISA) using neutralizing monoclonal antibodies (MAbs) against Akabane virus (AKAV) was developed to detect antibodies to AKAV in cattle sera. The performance of the test using 7 different competitor MAbs was evaluated in sequential serum samples and sera from cattle infected with various bovine arboviruses. The dynamics of the antibody response expressed by percentage of inhibition (PI) in C-ELISA coincided with those of neutralizing antibody titers in sequential serum samples from 2 cattle experimentally infected with AKAV. The value of PI in C-ELISA for convalescent sera from cattle infected with arboviruses correlated with the neutralizing antibody titer to AKAV but was unaffected by the antibodies to other arboviruses. In the validation experiment of C-ELISA using 286 bovine sera previously examined for the AKAV antibody by serum neutralization (SN) test, the relative specificity of C-ELISA was more than 98%, whereas the relative sensitivities of individual MAbs ranged from 49% to 82.2%. Overall agreement between C-ELISA and the SN test varied from 72% to 90% depending on the MAb. These results suggest that the C-ELISA is acceptable as a rapid and specific method for detecting antibodies to AKAV and is a potential alternative to the SN test. 相似文献
3.
Kakizaki T Hamada N Sakashita T Wada S Hara T Funayama T Hohdatsu T Natsuhori M Sano T Kobayashi Y Ito N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):605-609
An analysis of ionizing radiation-induced damage in peripheral lymphocytes has been employed to predict the prognosis of radiotherapy in terms of toxicity in normal tissues. Therefore, understanding the sensitivity of lymphocytes to high linear energy transfer (LET)-charged particles would be indispensable for utilizing charged particle therapy in veterinary medicine. However, the availability of such information is very limited. This study aimed to compare the radiosensitivity of feline T lymphocytes to gamma-rays (0.2 keV/microm) and 4 different types of charged particles with LET values ranging from 2.8 to 114 keV/microm. It was observed that the relative biological effectiveness, inactivation cross-section, and isodose-induced apoptosis increased in an LET-dependent manner. On the other hand, no difference in apoptosis frequency was observed in the cells exposed to an isosurvival dose of all the radiation types tested. This is the first study that demonstrates the LET dependence of cell killing and apoptosis induction in feline T lymphocytes. Our results suggest that lymphocytes can be effectively used to predict the prognosis of charged-particle therapy in cat patients. 相似文献
4.
The phospholipid class composition, fatty acid composition and phospholipase A1 (PLA1) activity from the ovaries of skipjack tuna were compared with those of six other species of marine fish. In the skipjack
ovaries, the lysophosphatidylcholine (LPC) proportion for the phospholipid, the docosahexaenoic acid (DHA) percentage for
the total fatty acids of the phospholipids and the PLA1 activity of the crude enzyme were the highest among those of the seven species. The optimum pH and temperature for the PLA1 activity of the crude enzyme from the skipjack ovaries were in the range of pH 6–7 and 20–30°C, respectively, and calcium
ions were not required. As a substrate, phosphatidylcholine was more easily hydrolyzed than phosphatidylethanolamine by this
enzyme, and the plasmalogen-type phospholipid was much lower than the acyl-type phospholipid. After a 6-h hydrolysis reaction
of the purified phospholipid extracted from the mixed ovaries of skipjack and yellowfin tuna by this enzyme, the LPC ratio
of the phospholipid increased from 20 to 72.6% and the percentage of DHA for the total fatty acids of the phospholipid also
increased. Thus, skipjack ovaries might possibly be used as a source of PLA1. 相似文献
5.
This study was performed to obtain information on the occurrence of multiple paternities in three species of viviparous Japanese
surfperch using allelic markers of microsatellite DNA loci. Direct evidence for multiple fertilizations was established by
reconstructing paternal genotypes from the progeny of gravid females. Multiple paternities were ascertained in five of 10
broods of Ditrema temmincki and in three of nine broods of Neoditrema ransonneti, but not in Ditrema viride. The number of patrilines detected in the progeny of D. temmincki and N. ransonneti females were two or three, respectively, as determined by the GERUD v2.0 algorithm for reconstructing parental genotypes
from half-sib progeny arrays. 相似文献
6.
Mizuho Takagi Yuko Yamaguchi Seiki Yamakawa Kazutoshi Tamura 《Journal of toxicologic pathology》2022,35(1):117
This report describes a case of spontaneous malignant pinealoma in a 90-week-old male Wistar rat. The tumor mass occurred in the deep cerebral parenchyma and no intact pineal gland was observed in the area between the posterior-dorsal median line of the cerebrum and the cerebellum. The tumor was characterized by a large nodular proliferation occupying the central area of the brain, extending from the dorsal surface to the base of the brain, corresponding to the thalamus. The tumor cells had round to irregular oblong nuclei approximately 5–17 μm in diameter and showed faintly or moderately eosinophilic cytoplasm and indistinct cell boundaries. Immunohistochemically, the tumor cells were positive for synaptophysin and partially positive for neuron-specific enolase (NSE). The tumor showed malignant features including cellular pleomorphism, high mitotic index, necrotic foci, and invasive and extensive growth and was, therefore, diagnosed as an extremely rare malignant pinealoma in the deep cerebral parenchyma. 相似文献
7.
Masaharu Komatsu Naoki Ichiyama Takashi Kurimoto Shota Takumi Kazuhiro Shiozaki Yasumasa Sugiyama Tatsuhiko Furukawa Seiichi Ando Saki Itonori Hiroaki Saito 《Fisheries Science》2013,79(2):313-320
We have previously established the method for isolation of ceramide aminoethylphosphonate (CAEP) from jumbo flying squid Dosidicus gigas. In this study, we performed a MTT assay to evaluate the safety of CAEP to the cell lines for the application to health food and supplements. The CAEP did not show any cytotoxicity to various HEK293-transfectant cells. Next, we elucidated the positive function of CAEP to the somatic cells. Recently, we have reported that hepatotoxin microcystin-LR was taken up into the hepatocytes mediated by hepatocellular uptake transporters OATP1B1 and OATP1B3, and the cells were induced cytotoxicity subsequently. Cytotoxicity of microcystin-LR to permanently OATP1B3-expressing HEK293-OATP1B3 cells rather than to HEK293-OATP1B1 cells was preferentially attenuated by CAEP in a concentration-dependent manner. In addition, the enzyme activity of serine/threonine phosphatase, which was inhibited by microcystin-LR, was recuperated by co-exposure to CAEP. Furthermore, microcystin-LR-induced cellular protein phosphorylation were disrupted by CAEP exposure. These results suggested that CAEP is a promising remedy and/or preventive medicine for liver damage with microcystin-LR. 相似文献
8.
Konomi Sakamoto Wataru Honto Masaharu Iguchi Nobuhiro Ogawa Kazuhiro Ura Yasuaki Takagi 《Fisheries Science》2009,75(1):91-98
This study examines the following in the Japanese mitten crab: (1) the structure of the exoskeleton with special reference
to its calcification; (2) the progression of post-molt cuticle formation and calcification. In the crab, the structure and
calcification state of the exoskeleton at the molt and during the inter-molt stage were similar to those of other crustaceans.
During the inter-molt, the exoskeleton consisted of four cuticle layers; the outermost epicuticle, the exocuticle, the endocuticle
and the innermost membrane layer. Intense calcification was observed in the exo- and endocuticle. At the molt, the synthesis
of the epi- and exocuticle was already complete, and the addition of the endocuticle began after the molt. Calcification of
the exocuticle initiated soon after the molt, but there was a delay between endocuticle matrix synthesis and calcification.
Histology showed that the process of calcification was similar to that in other crustaceans. However, calcium concentrations
within the exoskeleton continued to increase and never reached the levels of the inter-molt stage at the end of the experiment.
This suggests that the Japanese mitten crab is relatively slow to calcify compared to other crustaceans. 相似文献
9.
Takanobu Goto Shusaku Takagi Toshiaki Ichiki Tadashi Sakai Makoto Endo Terutoyo Yoshida Masaharu Ukawa Hisashi Murata 《Fisheries Science》2001,67(1):58-63
SUMMARY: The aim of this study was to investigate the cause of green liver in red sea bream fed substitute protein diets. Red sea bream Pagrus major was given either of the following diets for 28 weeks: (1) control diet (50% fish meal), (2) low level fish meal diet (15% fish meal), and (3) non-fish meal diet (0% fish meal). The green liver was observed in all groups tested, but the incidence was much higher in the experimental diet groups. The feeding of substitute protein diets reduced plasma triglyceride and cholesterol levels. However, there was no significant difference in plasma hepatic enzyme activities and plasma bile salts concentration among the treatments. Fish fed the substitute protein diets showed low hepatic taurine levels with an appearance of a biliverdin in the liver. Moreover, the proportion of ditaurobilirubin to total biliary bile pigments was significantly lower in fish fed the substitute protein diets. These data indicate that feeding of substitute protein diets did not induce any cholestatic hepatobiliary obstructions and that the low hepatic taurine level was one of the probable factors responsible for the occurrence of green liver in red sea bream fed substitute protein diets. 相似文献
10.
To elucidate the normal development and growth of Mongolian gerbils, the timing and sequence of the appearance of ossification centres within foetuses were investigated. Average foetus body weight and tail length on the 15th day of pregnancy were found to be 0.042 g and 6.2 mm, respectively. For the 25th day of pregnancy, these measurements were 2.601 g and 29.4 mm, respectively. The body weight and tail length of foetuses increased significantly from the 20th day of pregnancy. Also, the first ossification centre of Mongolian gerbil foetuses appeared in the clavicle at the 17th day of pregnancy. The order in which ossification centres appeared in Mongolian gerbils resembles the pattern for rats and mice, except in some areas of the skull. Except for the clavicle, in which ossification centres appeared rather early, most ossification centres appeared in the last quarter of pregnancy. These results suggest that Mongolian gerbil foetal development is near completion by the last gestational stage. Also, ossification centres appear sooner in the skull and nasal bones than in other rodents. 相似文献