Towards developing resistance to chickpea chlorotic dwarf virus through CRISPR/Cas9-mediated gene editing using multiplexed gRNAs

Journal of Plant Diseases and Protection - Mastreviruses are an emerging group of viruses transmitted by leafhoppers and infect both monocot and dicot plants. Chickpea chlorotic dwarf virus...     

Impacts of Institutional Reforms on Irrigated Agriculture in Pakistan

Poor functioning of theirrigation system in Pakistan has been asource of concern for the last few decades,during which time it has been the subject ofconsiderable external assistance andinternal policy reforms. Consequently, thegovernment of Pakistan introducedinstitutional restructuring in irrigationand drainage subsectors to impartnecessary improvements. Under thesereforms, management at secondary canallevel has recently been handed over to theFarmers Organizations (FOs) of selecteddistributaries in pilot areas. This studywas designed to evaluate a farmer-manageddistributary in southern Punjab. Fieldmeasurements revealed that hydraulicaspects of the irrigation service deliveredby the FO management has been significantlyimproved and as a result highlyproportionate and equitable waterdistribution was observed, particularly atthe tail reaches of the distributary. Dueto effective FO management farmers'interventions to increase the outletdischarge by illegal means has been almosteradicated. As a result of theseimprovements imparted by the FO in systemmanagement, the extent of irrigated area hasincreased on average by 6 to 7%, evenunder severe drought-like conditionsprevailing in the country during recentyears. Further cost recoveries haveincreased by an amount of 14% for summerand 23% for winter growing seasons,respectively, from the irrigated area of thedistributary. Thus experience from Hakra4-R distributary has shown that even underundesirable natural circumstancesIrrigation Management Transfer (IMT) wasquite effective in achieving the keytargets of the institutional reformslaunched at pilot scale in the country.     

Natural products as pesticides: Two examples of stereoselective synthesis

The strategy and synthetic efforts leading to efficient stereoselective syntheses of thiangazole, a tris-thiazolinyl-oxazole metabolite isolated from Polyangium spp., and of hydantocidin, a spironucleoside metabolite isolated from Streptomyces hygroscopicus, are discussed.     

Bacterial diversity of tilapia (Oreochromis niloticus) cultured in brackish water in Saudi Arabia

The bacterial flora occurring in brackish pond water, sediment, gills and intestine of healthy tilapia cultured in Saudi Arabia were estimated both quantitatively and qualitatively, and the isolates were identified to genus or species level. Total viable count of bacteria ranged from 1.4±1.5×10³ to 8.6±2.7×10³ cfu ml⁻¹; 1.2±3.1×10⁶ to 7.3±1.1×10⁷ cfu g⁻¹; 8.7±1.9×10⁵ to 2.1±0.9×10⁶ cfu g⁻¹; and 2.8±2.4×10⁷ to 1.0±1.6×10⁸ cfu g⁻¹ in the pond water, sediment, gills and intestine of brackish water tilapia, respectively. In total, 19 bacterial species were identified. The bacteria were predominantly Gram-negative rods (87%). Pond water and sediment bacteria influenced the bacterial composition of gills and intestine of tilapia. In contrast to gill bacteria, more diversification was observed in intestinal bacteria. The predominant (prevalence >10%) bacterial species were Vibrio parahaemolyticus, Vibrio carchariae, Vibrio alginolyticus, Chryseomonas sp., Vibrio vulnificus, and Streptococcus sp. in all the populations with the exception of the sediment population where Streptococcus sp. was replaced by Shewanella putrefaciens. Vibrio spp. (58% of the total isolates) dominated the total bacterial population.     

Effects of ambient gaseous pollutants on photosynthesis,growth, yield and grain quality of selected crops grown at different sites varying in pollution levels

In recent decades, ambient gaseous pollution has increased due to anthropogenic activities worldwide. The studies to evaluate the adverse effects of ambient pollutants on commonly grown food crops are still limited, especially in Asian countries like Pakistan. The present study was conducted to measure the ambient pollutants in different sites of Faisalabad and their impact on growth and yield of wheat, mung bean and peas. Plants were grown in pots and placed at three sites named as control (Wire house of Government College University, Faisalabad), low pollution (LP) (Farm Area of Ayub Agricultural Research Institute) and high pollution (HP) (GT Chowk, Faisalabad) sites. Results showed that ambient ozone (O₃) concentration was highest at HP site followed by LP site and was below AOT40 in control site. Ambient pollutants caused foliar injury in crops and decreased plant height, leaf area, biomass and grain yield. Pollutants caused a reduction in photosynthetic pigments, stomatal conductance and net photosynthetic rate and grain protein contents in all three crops. In conclusion, the ambient O₃ level was highest at HP site, this current O₃ level and other pollutants decreased the growth and yield of important food crops.     

Effect of supplementation of mustard oil cake on intake,digestibility and microbial protein synthesis of cattle in a straw-based diet in Bangladesh

The objective of this study was to analyse the effects of different levels of rumen-degradable protein (RDP) on intake, digestibility and microbial protein synthesis by supplementing mustard oil cake (MOC) on rice straw-based diet of cattle (Bos indicus) in Bangladesh. A 4 × 4 Latin square design was applied. Four diets having constant energy (7.0 MJ/kg of dry matter (DM)) with varying levels of RDP (M ₀ = 4.1 g/MJ (control), M ₁ = 6.3 g/MJ, M ₂ = 8.3 g/MJ and M ₃ = 12.4 g/MJ of metabolizable energy (ME)) were received by each animal for a period of 28 days. A metabolism trial was conducted for 7 days. Results indicate that with increasing levels of RDP, crude protein (CP) and RDP intake increased significantly (P < 0.01). The significant (P < 0.01) increase in digestibility values are obtained for DM, organic matter, CP and digestible organic matter in the rumen. The digestibility of neutral detergent fibre and acid detergent fibre was also increased significantly (P < 0.05). The total nitrogen (N), ammonia-N and total volatile fatty acids increase significantly (P < 0.01) while the rumen pH increased from M ₀ to M ₂ and decreased thereafter. The efficiency microbial N intake increased significantly (P < 0.01) but showed a curvilinear response with higher RDP level (12.40 g/RDP/MJ ME). This study concludes that supplementation of RDP from MOC enhances the intake, digestibility and microbial protein synthesis which ultimately increases utilization of low-quality feed resources that can be used for developing cost-effective feeding systems on a straw-based diet in tropical regions.     

Serosurvey of five viruses in chickens on smallholdings in Bangladesh

A serologic survey was undertaken in chickens in smallholdings in Bangladesh for avian influenza A virus (AIV), egg drop syndrome '76 virus (EDS'76V), infectious bronchitis virus (IBV), Newcastle disease virus (NDV) and reovirus (RV) in three phases: January 2002-May 2003, September 2003-August 2004, and August 2005-March 2006. Four hundred thirty-six sera collected in the 2nd phase, 295 in the first phase, 755 in the 1st plus 2nd phases and 295 in the 1st phase were investigated for AIV, EDS'76V, IBV and RV, respectively, using enzyme linked immunosorbent assays. All 854 sera collected in the three phases were screened for NDV using hemagglutination inhibition test. In chickens 20% were seropositive to AIV, 3% to EDS'76V, 74% to IBV, 88% to NDV, and 47% to RV. The seroprevalence in flocks was 23% to AIV, 6% to EDS'76V, 79% to IBV, 89% to NDV and 56% to RV. Twenty-five percent chickens had > or = 10log(2)HI titers to NDV.     

Automation in Agriculture by Machine and Deep Learning Techniques: A Review of Recent Developments

Precision Agriculture - Recently, agriculture has gained much attention regarding automation by artificial intelligence techniques and robotic systems. Particularly, with the advancements in...     

Lodging-related morphological traits of hybrid rice in a tropical irrigated ecosystem

Hybrid rice (Oryza sativa L.) has been proven to be effective in increasing yield potential, but lodging often limits its yield in high-yielding environments. This study was conducted to determine genotypic variation in lodging resistance and lodging-related morphological traits among hybrid and non-hybrid check varieties, and to identify the key morphological traits associated with lodging resistance. Lodging behavior of 16 rice genotypes, including 12 hybrids and four inbreds, was studied at the International Rice Research Institute farm during the 2004 wet season (WS) and 2005 dry season (DS). Grain yield and visual score of lodging were determined at maturity. Lodging-related morphological traits were measured at 30 days after flowering. Large genotypic differences in lodging-related morphological traits were observed. Among these traits, dry weight per unit length, breaking resistance, and lodging index (bending moment/breaking resistance × 100) of lower internodes were significantly correlated with visual score of lodging. Several hybrids demonstrated high lodging resistance although their average plant height was over 120 cm. The lodging-related morphological traits and visual score of lodging in these hybrids were comparable with those of the non-hybrid check varieties with strong lodging resistance. These results suggest that increases in dry weight per unit length and breaking resistance of lower internodes are primary targets for reducing lodging index, thus improving overall lodging resistance of hybrid rice in a breeding program.