Skeletal muscle characteristics in young trained and untrained standardbred trotters.

Muscle biopsies were taken from the middle gluteal muscle of 28 Standardbred trotters, 3-4 years of age. The 13 horses in Group T were trained consistently from 18 months of age, whereas the 15 horses in Group UT were not exposed to any systematic training before 3 years of age. Group T horses had a lower percentage of Type IIB fibres (31%) than did Group UT horses (39%). Citrate synthase (CS) activity, representing oxidative capacity, was higher in Group T (72 mmol kg-1 min-1) than in Group UT (47 mmol kg-1 min-1). Biopsies were taken from 4 horses in each group when they were foals and then annually until 3-4 years of age. Results from this study indicate that regular training of Standardbreds from 18 months of age resulted in increased CS activity and a decrease in the percentage of Type IIB fibres. This study shows that training, not growth, is the main factor that induces a high oxidative capacity and a high Type IIA/IIB fibre ratio in muscle of Standardbred trotters.     

Cross protection studies on Bordetella bronchiseptica in mice using an intracerebral challenge model.

Protective activities of heat-inactivated (60 degrees C for 30 min) merthiolate preserved Bordetella bronchiseptica and B. pertussis bacterins were compared in intraperitoneally immunized mice challenged intracerebrally (i.p./i.c.) or intraperitoneally (i.p./i.p.). In the i.p./i.c. assay (Kendrick test), a B. pertussis bacterin protected mice against challenge with B. pertussis 18-323, as well as against phase I cytotoxic and non-cytotoxic strains of B. bronchiseptica. A B. bronchiseptica bacterin, prepared from a phase I cytotoxic strain, gave protection against two phase I B. bronchiseptica strains, irrespective of their cytotoxin-production. A non-cytotoxic phase I strain of B. bronchiseptica elicited protection against the homologous strain only. Neither cytotoxic nor non-cytotoxic B. bronchiseptica strains protected mice challenged with B. pertussis 18-323. Vaccines prepared from phase III strains of B. bronchiseptica were not protective at all against any of the challenge strains. No such differences in the protective activities of the bacterins could be detected by the i.p./i.p. method. They seem to cross-protect equally well. The results indicate that the Kendrick test may be useful in testing potency of different B. bronchiseptica bacterins.     

配筋混凝土砌块高层设计时应注意的几个问题

针对配筋混凝土砌块高层设计,提出砌块空腔墙的应用,注意合理设置水平配筋,配筋砌块深梁,控制底框房屋层间抗侧刚度比及高层墙体的裂缝问题.     

Taxonomic and Bioactivity Characterizations of Mameliella alba Strain LZ-28 Isolated from Highly Toxic Marine Dinoflagellate Alexandrium catenella LZT09

Microalgae host varied microbial consortium harboring cross-kingdom interactions with fundamental ecological significance in aquatic ecosystems. Revealing the complex biofunctions of the cultivable bacteria of phycosphere microbiota is one vital basis for deeply understanding the mechanisms governing these dynamic associations. In this study, a new light-yellow pigmented bacterial strain LZ-28 was isolated from the highly-toxic and harmful algal bloom-forming dinoflagellate Alexandrium catenella LZT09. Collective phenotypic and genotypic profiles were obtained to confidently identify this strain as a new Mameliella alba member. Comparative genomic analysis showed that strain LZ-28 shared highly similar functional features with other four marine algae-derived M. alba strains in spite of their distinctive isolation sources. Based on the bioactivity assaying, the mutual growth-promoting effects between bacterial strain LZ-28 and algal strain LZT09 were observed. After the culture conditions were optimized, strain LZ-28 demonstrated an extraordinary production ability for its bioflocculanting exopolysaccharides (EPS). Moreover, the portions of two monosaccharides glucose and fucose of the EPS were found to positively contribute to the bioflocculanting capacity. Therefore, the present study sheds light on the similar genomic features among the selected M. alba strains, and it also reveals the potential pharmaceutical, environmental and biotechnological implications of active EPS produced by this new Mameliella alba strain LZ-28 recovered from toxic bloom-forming marine dinoflagellate.     

滴头流道中颗粒物质运移动态分析与CFD模拟

采用CFD模拟技术,对迷宫式滴头流道内不同质量浓度的悬浮颗粒运动情况进行数字化模拟计算,并采用两相流动力学理论对迷宫式滴头结构尺度下的悬浮颗粒的随流性进行了分析,研究结果表明,采用DPM模型对悬浮颗粒分布特点的模拟结果与已有试验数据吻合度较高,可以作为模拟的手段;水流中颗粒质量浓度对滴头内部流场有着较强的反作用,应在模拟过程中予以重视;根据Stokes数的计算结果,10%左右的颗粒随流性差,可以认为导致滴头堵塞的物质主要来自于这一部分颗粒。     

车用永磁式缓速器设计中漏磁影响因素分析

以优化设计和减少漏磁为目标,运用磁路分析法建立永磁式缓速器的漏磁场模型,得到设计参数的解析式。由于参数之间的复杂函数关系,采用区间数学算法来优化永磁式缓速器的设计参数,将影响漏磁系数的非线性方程组化简为线性方程组。选取满足设计要求的目标参数——转子鼓磁感应强度Bm、气隙磁感应强度Bg,ave、漏磁系数K的最佳取值区间。使用区间迭代法,求出自变量参数的取值区间和权数pi。运用Matlab语言编程直接搜索合理的设计参数,减少了计算量和循环次数。三维有限元漏磁分析和试验均证明了设计参数选取的合理性。结果表明：永磁体高度HPM、气隙ge对漏磁系数K影响较大;区间数学Tschernikow算法在永磁式缓速器设计中是有效的。     

电涡流缓速器对车辆制动稳定性的影响分析

修正车辆理想制动力分配曲线使之适用于装用电涡流缓速器的车辆,对装用电涡流缓速器的车辆制动稳定性进行了定性分析,并且提出了增加前后制动器制动力的分配系数来提高装用缓速器后的车辆制动稳定性的技术措施.对给定参数的车辆的定量分析表明,提出的技术措施能扩大车辆同步附着系数的范围,降低车辆后轮先抱死的可能性,提高车辆的制动稳定性.     

Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickensOA

Background: Many researches about in ovo feeding(IOF) of vitamin C(VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems.Results: Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase(GLO) expression was lower during pre-hatch development than that during p...     

稀释液中添加谷氨酰胺对绒山羊冷冻精液品质的影响

在绒山羊冷冻精液稀释液中分别添加0mmol/L、2.5mmol/L、5.0mmol/L、7.5mmol/L和10.0mmol/L五个梯度的谷氨酰胺,以确定冷冻效果最好的谷氨酰胺添加浓度,优化绒山羊冷冻稀释液配方。结果表明:添加5.0mmol/L谷氨酰胺组精子的活率、顶体完整率和生存指数均极显著或显著地高于其他试验组(P0.01,P0.05),而畸形率极显著或显著低于其他试验组(P0.01,P0.05);在脂质过氧化反应和抗氧化酶活性方面,添加5.0 mmol/L组MDA浓度极显著低于其他试验组(P0.01),而CAT活性极显著高于其他试验组(P0.01)。总之,在稀释液中添加5.0mmol/L谷氨酰胺,在冷冻—解冻过程中能起到很好的保护精子的作用。