甜菜坏死黄脉病毒(BNYVV)75kDa通读蛋白基因及其54kDa片段的克隆与表达

 以甜菜坏死黄脉病毒(BNYVV)内蒙分离物的总RNA为模板,通过反转录和PCR扩增获得75kDa通读蛋白基因54kDa片段的目的片段。     

Effect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense

This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense.     

左旋咪唑在生长育肥猪驱虫中的应用研究

本文探讨了5%左旋咪唑注射液按10mg/kg 体重剂量的不同驱虫次数对生长育肥猪驱虫效率、中毒反应及生产性能的影响。结果表明,1)在60～240日龄全期试验内,分别于60和150日龄各驱虫1次的试验Ⅰ组猪日增重极显著高出对照组(不驱虫)的23.9%(p<0.01);也显著高于只在60日龄驱虫1次的试验Ⅱ组(p<0.05),尽管Ⅱ组猪日增重显著高于对照组14.5%(p<0.05)。2)用药后,猪只应激毒性反应为100%,但均能自然康复。3)2次驱虫的效率比1次驱虫的高,且彻底。因此,用左旋咪唑给生长育肥猪驱虫,宜进行2次(分别于60和150日龄),可显著改善生长性能和提高经济效益,适宜在农(山)区养猪中推广。     

发展我国洪水保险的制度思考——美国洪水保险制度的借鉴与启示

洪水保险是一项重要的非工程防洪措施.美国是世界上最早建立国家强制性洪水保险体制的国家,经过艰难曲折的探索,闯出了一条成功之路.我国的洪水保险体制还处于积极探索阶段.中美两国尽管国情差异较大,但以洪水保险作为推进洪泛区洪水风险管理的经济手段,并以立法和国家计划等措施来保障实施的作法是值得借鉴的.本文就美国洪水保险制度的发展与沿革,寻找对我国建立洪水保险制度一些有益的启示,并就此提出了一些建设性意见.     

HUDACAPP— 一种工艺规程自动设计系统

本文介绍浦沅工程机械总厂准柔性制造系统(P-FMS)中加工箱体零件所用的一个工艺规程自动设计系统—HUDACAPP,阐述了系统的原理、结构和实现.给出了实例.本系统能按零件输入信息自动生成工艺过程,选择机床、夹具、刀具、量具、切削参数以及计算工时,并能与P-FMS的实时管理调度软件联接,提供所需的资料.     

康绿功臣植物源农药防治苹果李始叶螨试验

以1．1％苦参碱康绿功臣粉剂、25％三氯杀螨醇乳油及73％克螨特乳油为供试药剂，在喀什垦区对苹果李始叶螨进行了小区及示范防治试验，结果表明，康绿功臣防治李始叶螨田间药效较好，具有安全、高效稳定、持效期长等优点，可以取代25％三氯杀螨醇。     

豫麦10号高产栽培技术调查总结

通过对小麦新品种豫麦1O号的观察,认为该品种在上蔡县增产潜力较大。为充分发挥其增产优势,从整地播种,施肥浇水,中、后期管理等方面提出了措施和建议。