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1.
I. Halila M. J. Cobos J. Rubio T. Millán M. Kharrat M. Marrakchi J. Gil 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(1):87-92
A second gene conferring resistance to the chickpea wilt pathogen, Fusarium oxysporum f. sp ciceris race 0, has been mapped to linkage group 2 (LG2) of the chickpea genetic map. Resistance to race 0 is controlled by two genes
which segregate independently; one present in accession JG62 (Foc0
1
/foc0
1
) and mapping to LG5 and the second present in accession CA2139 (Foc0
2
/foc0
2
) but remaining unmapped. Both genes separately confer complete resistance to race 0 of the wilt pathogen. Using a Recombinant
Inbred Line (RIL) population that segregated for both genes (CA2139 × JG62) and the genotypic information provided by two
markers flanking Foc0
1
/foc0
1
ten resistant lines containing the resistant allele Foc0
2
/foc0
2
were selected. Genotypic analysis using these ten resistant lines paired with ten susceptible RILs, selected in the same
population, revealed that sequence tagged microsatellite sites (STMS) markers sited on LG2 were strongly associated with Foc0
2
/foc0
2
. Linkage analysis, using data from two mapping populations (CA2139/JG62 and CA2156/JG62), located Foc0
2
/foc0
2
in a region where genes for resistance to wilt races 1, 2, 3, 4 and 5 have previously been reported and which is highly saturated
with tightly-linked STMS markers that could be used in marker-assisted selection (MAS). 相似文献
2.
3.
Guil-Luna S Sánchez-Céspedes R Millán Y De Andrés FJ Rollón E Domingo V Guscetti F Martín de Las Mulas J 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2011,25(3):518-523
Background: Progesterone receptor (PR) antagonist aglepristone (RU534) has been used successfully for pregnancy termination and therapy of pyometra, vaginal tumors, and mammary hyperplasia in bitches and queens. All of these conditions share with canine mammary carcinomas the expression of PR. Objectives: To study the effect of RU534 on proliferation and apoptosis in canine mammary carcinomas in relation to PR expression. Animals: Twenty‐seven nonspayed bitches with mammary carcinomas were treated with either 2 doses of 20 mg/kg RU534 (n = 22, RU534‐treated group) or oil placebo (n = 5, control group) on days 1 and 8. Methods: Tumor samples were collected before (day 1) and after (day 15) treatment for immunohistochemistry. PR expression, proliferation index (PI), and apoptotic index (AI) were determined using antibodies against PR, Ki67, and cleaved lamin A/C antigens, respectively. The effect of treatment on these parameters was analyzed. Results: Differential expression of PR between day 1 (59.1% PR‐positive tumors) and day 15 (36.4% PR‐positive tumors) was observed in RU534‐treated tumors exclusively. After RU534 treatment, mean PI was significantly decreased in PR‐positive but unchanged in PR‐negative RU534‐treated tumors. A reduction of ≥20% in PI was found in 61.5% of RU534‐treated tumors with PR expression. Conversely, no effect on AI was observed after RU534 treatment. Conclusions and Clinical Importance: Neoadjuvant RU534 treatment had PR expression‐related inhibiting effects on proliferation of canine mammary carcinoma cells. 相似文献
4.
5.
A Clemente J Vioque R Sanchez-Vioque J Pedroche F Millán 《Journal of agricultural and food chemistry》1999,47(9):3776-3781
Chickpea protein isolate was used as starting material for the production of hypoallergenic protein hydrolysates. Western blotting of the protein isolate showed that IgE in sensitized patient sera strongly bound to the basic polypeptidic chains and recognized the acidic ones of 11S globulin. During the hydrolysis process by the individual and/or sequential action of endo- and exoproteases, a high reduction of antigenic activity was observed. Results suggest that the presence of intact or partially hydrolyzed basic polypeptide chains of 11S globulin are responsible for the formation of IgE complexes in protein hydrolysates obtained by exoprotease treatment; however, the digestion of these polypeptide chains by individual action of endoprotease caused a high loss of antigenic activity. The most effective reduction of antigenicity, >90%, was observed in extensive hydrolyzed chickpea proteins obtained by sequential treatment with endo- and exopeptidases. This chickpea protein hydrolysate could be useful for the elaboration of specialized hypoallergenic food products. 相似文献
6.
Nuria Castell Enrique Mantilla Ariel F. Stein Rosa Salvador Millán Millán 《Water, air, and soil pollution》2010,209(1-4):61-79
The impact of atmospheric industrial emissions on secondary pollutant formation depends on many factors; one of the most important being the environmental setting in which the industry is located. The environmental setting affects an industry’s impact on ozone levels through both the air mass dispersion (a function of topography and wind patterns) and the emissions of organic volatile compounds (VOC) and nitrogen oxides (NO x ) in the area. This model-based study shows how the sensitivity of surface ozone changes with the choice of source location. For the analysis, seven points distributed along the Tinto–Guadalquivir Basin (in the Southwestern Iberian Peninsula) were selected. This area is characterized by the close proximity of natural environments and crop fields to cities, roads, and industrial areas with high NO x emissions. Natural VOC emissions represent more than 60% of the total non-methane volatile organic compounds emitted in the study area. The results reveal that the largest increases in ozone levels are produced when the industry is located both far away from NO x emission sources and near to biogenic VOC emissions. Furthermore, the highest increases over the hourly and 8-hourly maximums, as well as the highest accumulated daily values, are found in areas characterized by high VOC/NO x emission ratios and NO x sensitivity. The study of the recurrent meteorological patterns along with the distribution of chemical indicators of the O3–NO x –VOC sensitivity allows the determination of the industry’s geographical impact on ozone levels. This information enables air quality managers to decide the future location of an industry minimizing its impact on smog levels. 相似文献
7.
Photooxidation of cumene (isopropylbenzene) and pyridine in dilute solution in natural waters gives products characteristic of reactions with alkylperoxy (RO(2).) and hydroxyl (HO.) radicals. On the basis of the rates of formation of the products, the average concentrations of RO(2). and HO. are estimated to be about 10(-9) and 10(-17) mole per liter, respectively. The concentration of RO(2). is large enough that, for some classes of reactive chemicals, oxidation can be an important process in natural waters. 相似文献
8.
Mill 《European Journal of Forest Research》1863,7(1):436-440
Ohne Zusammenfassung 相似文献
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10.