全文获取类型
收费全文 | 250篇 |
免费 | 20篇 |
专业分类
林业 | 24篇 |
农学 | 15篇 |
39篇 | |
综合类 | 7篇 |
农作物 | 6篇 |
水产渔业 | 34篇 |
畜牧兽医 | 122篇 |
植物保护 | 23篇 |
出版年
2024年 | 2篇 |
2023年 | 5篇 |
2022年 | 10篇 |
2021年 | 14篇 |
2020年 | 13篇 |
2019年 | 20篇 |
2018年 | 17篇 |
2017年 | 14篇 |
2016年 | 19篇 |
2015年 | 10篇 |
2014年 | 23篇 |
2013年 | 9篇 |
2012年 | 21篇 |
2011年 | 22篇 |
2010年 | 6篇 |
2009年 | 9篇 |
2008年 | 10篇 |
2007年 | 15篇 |
2006年 | 6篇 |
2005年 | 3篇 |
2004年 | 2篇 |
2003年 | 1篇 |
2002年 | 3篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1980年 | 2篇 |
1971年 | 2篇 |
排序方式: 共有270条查询结果,搜索用时 0 毫秒
1.
2.
A single-step duplex polymerase chain reaction (PCR) technique and traditional microscopic examination of haemolymph smears were used to detect Babesia bigemina and/or Babesia bovis infection in engorged female ticks of Boophilus microplus recovered from calves raised in an endemic area of the State of Minas Gerais, Brazil. In the PCR amplification of tick-derived DNA, pairs of oligonucleotide primers specific for a 278-bp sequence from B. bigemina and for a 350-bp sequence from B. bovis were used conjointly. The microscopic examination of haemolymph revealed that 16.7% of the engorged ticks were infected with Babesia spp., although no significant differences (rho > 0.05) were found in the infection rate of ticks collected from calves of different age groups. PCR analysis showed that 77.8% of the engorged ticks whose haemolymph contained sporokinetes were infected with B. bigemina, 7.8% with B. bovis and 14.4% with both protozoan species. However, the PCR assay further revealed that, amongst the engorged female ticks whose haemolymph was apparently negative for the presence of sporokinetes, 15.6% were infected with B. bigemina, 2.2% with B. bovis and 10.0% with both species. The duplex PCR method is thus more efficient and sensitive than the microscopic assay and also permits facile identification of the protozoa species present in engorged female ticks. 相似文献
3.
Luiz Felipe Ramos Carvalho Cristiano Barros de Melo Luiza Seixas Concepta McManus 《Tropical animal health and production》2014,46(3):495-502
The aim of this study was to define the differences between the Brazilian states that export and do not export meat to the European Union (EU) and to identify the variables that are important to meet the export requirements. Infrastructure and computerization of the control of animal transit in Brazil that impact on regional health status were evaluated and linked to other variables such as status for foot and mouth disease (FMD) and qualification to export meat to EU. Variables related to transit control of bovines implemented by the state agencies of animal health and inspection in each Brazilian state were evaluated. Using a discriminant analysis, four variables were selected that explained the variation between Brazilian states that were “free” and “not free” of FMD while another four were selected to explain the variation between the zones “approved” and “not approved” to export meat to the EU, including number of official veterinarians, total transit of bovines and buffaloes, total number of animal transit certificates issued for bovine and buffaloes at the state or zone level, and total number of municipalities in the state or zone. It was possible to correctly discriminate between “free” and “not free” FMD states or zones. Variables related to animal transit are important in assessing the state for the classification of animal health situation and for EU approval for the exportation of meat. 相似文献
4.
Oliveira JB Montoya J Romero JJ Urbina A Soto-Barrientos N Melo ES Ramos CA Araújo FR 《Veterinary parasitology》2011,177(3-4):359-365
Bovine anaplasmosis is endemic and occurs in almost all areas of livestock production of Costa Rica. The aim of this study was to determine the seroprevalence and risk factors of anaplasmosis in dairy farms of Costa Rica by the recombinant truncated MSP-5 (rMSP-5) enzyme-linked immunosorbent assay (ELISA). Serum samples were obtained from 733 cattle from 20 commercial dairy herds of Costa Rica. The overall seroprevalence was 37.2% and herd seroprevalence ranged from 20.0 to 72.0%. The age-specific seroprevalence was 49.3% in young and 33.4% in adult animals. The main risk factors associated with seroprevalence were season of occurrence of clinical cases (rainy season) (OR=22.8), presence of tabanids (OR=9.5) and stable flies (OR=6.2), stable flies control measures (OR=3.2), non-use of ear tattoos (OR=2.8), interval of veterinary visit (≤ 60 days) (OR=2.7), altitude of the farms (<800 masl) (OR=2.6) and age (<2 years) (OR=1.8). The results indicated that exposure of cattle to Anaplasma marginale is common in dairy herds of Costa Rica and endemic instability situation probably is due to inadequate vector control. 相似文献
5.
Brenda Colleen Clifton-Cardoso Marcelo Schramm Mielke José Roberto De Melo Rondinele Nascimento Querino 《New Forests》2008,35(1):15-31
Dimorphandra jorgei MF Silva (Caesalpinaceae) and Swartzia macrostachya Benth. var. riedelii Cowan (Fabaceae) are tree species from the southern Bahian Atlantic Forest, identified for the restoration of degraded areas.
The objective of this research is to study their seed germination and seedling growth to develop simple methods for seedling
production in small scale nurseries. Because the species have distinct dormancy and germination characteristics, the seeds
were submitted to different treatments. D. jorgei seeds were immersed in hot water, scarified and stored for 12 weeks in the laboratory environment (25°C). S. macrostachya seeds were submitted to different combinations of drying and storage in the laboratory environment or refrigerator (4°C).
Seedlings were submitted to two light treatments for 3 months: partial shade (52% of full sun) and full shade (12% of full
sun). In D. jorgei, the highest germination occurred for scarified seeds (87%) and for seeds stored for 12 weeks and then scarified (69%). In
S. macrostachya the undried control seeds had the highest germination, followed by that of the refrigeration storage treatment, without predrying
(99% and 97%, respectively). The average values for the growth parameters were greater in full shade for D. jorgei, while S. macrostachya seedlings showed greater growth rates in partial shade. Two months growth is recommended before D. jorgei seedlings are of the sufficient size and leaf number for field planting. On the other hand, a period of 4 months is recommended
for S. macrostachya, due to its relatively slow growth. 相似文献
6.
AISB Villaverde EG Fioratti FC Landim-Alvarenga JC de Carvalho Balieiro CM Melo FO Papa MD Lopes 《Reproduction in domestic animals》2009,44(S2):309-312
This study was aimed at assessing the changes in sperm motion patterns and the percentage of acrosome reaction (AR) in domestic cat semen after treatment with either ionomycin or progesterone (P4 ). Ten ejaculates were collected from five tomcats using an artificial vagina, and were diluted, centrifuged and resuspended in a capacitation medium. Samples were evaluated and divided into seven equal aliquots and, after 2 h at 25°C, were incubated for 30 min at 38°C in 5% CO2 and then analyzed. Computer-assisted sperm analysis and a combination of three fluorescent probes were used to assess sperm plasma, acrosomal membrane integrity and mitochondrial transmembrane potential. Thirty minutes after the start of incubation, P4 was added (10 μg/ml) to the P1 group. Groups P2 and P3 were supplemented with P4 (10 and 20 μg/ml, respectively) only after 2 h of incubation, and groups I1 and I2 were supplemented with ionomycin (4 and 8 μ m , respectively) 2 h after incubation. Group E was supplemented with ethanol (0.6%) at 2 h after incubation and group C received no supplementation. Ionomycin and P4 treatments led to a hyperactivation-like sperm motion and an increase (p < 0.05) in the percentage of AR. Although a higher (p < 0.05) percentage of AR was obtained in group I2 when compared with all P4 groups, a decrease (p < 0.05) in total and progressive motility was observed in I2 group. As I1 group was similar to I2 to induce AR without diminishing sperm motility, we can conclude that ionomycin at 4 μ m seems to be more suitable to trigger AR in domestic cat sperm. 相似文献
7.
8.
Summary The main constraint of using Ogura male-sterile cytoplasm in Brassica is the induction of leaf yellowing at low temperature and the low seed set. But whether or not the cytoplasm can disturb the general commerical performance of the plants is not well-known. Therefore, this work was carried out with the purpose of evaluating Ogura cytoplasmic male-sterile cabbage hybrids and compare them to genomic similar male-fertile ones and to the most popular cabbage hybrid cultivated in Brazil. Harvest data showed an effect of the cytoplasm on reducing plant and head weight, core length and head length and width, although not altering heading index, shape and core indexes, nor head compactness. On the other hand, it was observed also that a careful selection of the parental lineages can produce male-sterile hybrids as good as their male-fertile analogues. All the evaluated hybrids were similar or better than the check for the characteristics under study.Abbreviations dat -
days after transplanting
- CNPH -
National Center for Vegetable Crops Research 相似文献
9.
Aeration strategy for controlling grain storage based on simulation and on real data acquisition 总被引:1,自引:0,他引:1
Daniela de Carvalho Lopes Jos Helvecio Martins Adílio Flauzino Lacerda Filho Evandro de Castro Melo Paulo Marcos de Barros Monteiro Daniel Maral de Queiroz 《Computers and Electronics in Agriculture》2008,63(2):140-146
In this work a new control strategy for grain aeration systems was proposed. This strategy was implemented in a software package called AERO and relates four conditions which depend on real time data acquisition and on the process simulation. The main advantage of AERO controller is that it can be used in different geographic regions, during different seasons and with different aeration systems, automatically adjusting its set points. The objectives of AERO controller are to equalize the temperatures inside the bin, to cool the grain mass whenever is possible and to maintain the moisture content under safe conditions. The results showed that the proposed strategy is efficient to achieve these objectives. Moreover, significant energy saving has been achieved with the AERO controller. 相似文献
10.
de Melo Rafaela Leitão Correia Dutra Júnior Wilson Moreira Palhares Liliane Olímpio de Moura Ferreira Débora Nathália de Aquino Rafael Santos Cordeiro Manso Helena Emília Cavalcanti 《Tropical animal health and production》2019,51(5):1057-1063
Tropical Animal Health and Production - The objective of this study was to evaluate the biochemical, behavioral, and physiological parameters in pregnant sows of native and improved crossbreeds... 相似文献