配筋混凝土砌块高层设计时应注意的几个问题

针对配筋混凝土砌块高层设计,提出砌块空腔墙的应用,注意合理设置水平配筋,配筋砌块深梁,控制底框房屋层间抗侧刚度比及高层墙体的裂缝问题.     

Second round of an interlaboratory comparison of SARS-CoV2 molecular detection assays used by 45 veterinary diagnostic laboratories in the United States

The COVID-19 pandemic presents a continued public health challenge. Veterinary diagnostic laboratories in the United States use RT-rtPCR for animal testing, and many laboratories are certified for testing human samples; hence, ensuring that laboratories have sensitive and specific SARS-CoV2 testing methods is a critical component of the pandemic response. In 2020, the FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) led an interlaboratory comparison (ILC1) to help laboratories evaluate their existing RT-rtPCR methods for detecting SARS-CoV2. All participating laboratories were able to detect the viral RNA spiked in buffer and PrimeStore molecular transport medium (MTM). With ILC2, Vet-LIRN extended ILC1 by evaluating analytical sensitivity and specificity of the methods used by participating laboratories to detect 3 SARS-CoV2 variants (B.1; B.1.1.7 [Alpha]; B.1.351 [Beta]) at various copy levels. We analyzed 57 sets of results from 45 laboratories qualitatively and quantitatively according to the principles of ISO 16140-2:2016. More than 95% of analysts detected the SARS-CoV2 RNA in MTM at ≥500 copies for all 3 variants. In addition, for nucleocapsid markers N1 and N2, 81% and 92% of the analysts detected ≤20 copies in the assays, respectively. The analytical specificity of the evaluated methods was >99%. Participating laboratories were able to assess their current method performance, identify possible limitations, and recognize method strengths as part of a continuous learning environment to support the critical need for the reliable diagnosis of COVID-19 in potentially infected animals and humans.     

芦荟饮料褐变的控制研究

研究了芦荟凝胶在制汁过程及芦荟饮料在加工过程中温度和时间等因数对芦荟饮料色泽的影响。结果表明,搅汁时间10min,杀菌温度85℃,杀菌时间25min是有效控制芦荟饮料褐变的最佳工艺参数。     

基于SRAP分子标记的11份柑橘种质材料遗传多样性分析

为分析11份柑橘种质材料的遗传多样性,本研究以7份沙糖橘种质以及4份其他柑橘种质为试验材料,利用SRAP分子标记进行分析,并用UPGMA法进行聚类.结果表明:160对引物组合筛选出20对引物组合扩增条带清晰,11份种质材料两两之间遗传相似系数在0.322 1～0.931 6之间.沙糖橘和金葵蜜橘间的遗传相似系数最大,为...     

用Nested-PCR检测广西黄皮与九里香黄龙病病原

摘要：运用nested-PCR技术对广西黄皮和九里香进行柑桔黄龙病（Citrus Huanglongbing, HLB）检测,结果表明：从部分黄皮和九里香样品抽提的DNA中,可扩增出一条特异性的400 bp条带,说明广西种植的黄皮和九里香均可感染黄龙病;在柑桔黄龙病疫区,黄皮和九里香黄龙病感染率分别为35.0%和40.0%。     

全球气候变化对松嫩草原土壤水分和生产力影响的研究

本文首先模拟计算松嫩草原土壤水量平衡,并结合未来气候情景估算松嫩草原土壤水分的变化。最后,在土壤水分研究的基础上,初步评估全球气候变化对松嫩草原生产力的影响。     

高钙日粮对蛋鸡生产性能及出壳雏体内锌含量的影响

生长期日粮钙>3.11%,可使蛋鸡性成熟期明显延缓,开产日龄推迟;其蛋锌及子代出壳雏血锌和多数组织锌含量明显降低,但胚胎及出壳雏鸡未见畸变.出壳雏血锌含量能反映体内锌状态,可作为其评价指标.产蛋期高钙(6.11%)或低钙(0.83%)日粮均可使产蛋性能及孵化率降低.     

哺乳动物卵母细胞孤雌激活的研究进展

本文概述了卵母细胞孤雌激活的研究历程,并从激活机制、激活方法及影响因素等方面对孤雌激活的研究意义和发展前景进行了综述。     

牛支原体和牛病毒性腹泻病毒二重二温式PCR检测方法的建立

为建立一种牛支原体(Mycoplasma bovis,MB)和牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)的快速鉴别诊断方法,针对MB的uvr C基因和BVDV的5'端非编码区(5'-UTR)保守基因序列,分别设计两对特异引物,并将三温式PCR扩增程序简化为二个温度梯度,建立了鉴别MB和BVDV的二重二温式PCR方法。该方法能同时扩增MB和BVDV,扩增产物大小分别为412和170 bp。特异性试验结果显示,该方法对参试的所有毒株只扩增MB和BVDV基因组,对其它牛病原体无扩增;敏感性试验结果显示,该方法最低能同时检测到104拷贝的两种目的核酸;干扰性试验结果显示,该方法能同时检测两个模板不同浓度的组合,试验结果不受模板影响。综上,本研究所建立的二重二温式PCR方法特异、敏感、快速、简便,可应用于MB和BVDV临床鉴别诊断和流行病学调查。     

紫花苜蓿根腐病活体整株接种试验

对19个苜蓿材料进行根腐病活体整株接种试验,结果表明:19个供试品种中有4个为免疫,7个为高抗,5个为中抗,3个为中感材料;从苜蓿秋眠数来看,秋眠数高的品种相应其抗病性强,而秋眠数低的品种易感染根腐病。