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Bacterial surfaces are generally negatively charged in the environmental pH-range and may therefore interact with metal cations, thus influencing the mobility of metals in soil and natural waters. In the present work the acid capacity and metal binding properties of Klebsiella oxytoca have been studied. A total acid capacity of 120 meq kg–1 was determined from potentiometric titrations of a suspension of starved bacteria in 0.1 M KC1. The adsorption of trace elements (Cs, Sr, Eu, serving as model systems, as well as Hg, Zn, and As) on starved bacteria was determined by a batch technique using radio tracers and with variation of pH (3 to 9). The adsorption followed the order Cs < sr,=" as=">< zn=">< hg=">< eu=" (at=" ph=" 5=" to=" 7).=" the=" uptake=" of=" eu=" was=" drastically=" increased=" when=" ph=" increased=" from=" 3.5=" to=" 4.5,=" indicating=" a=" surface=" complexation=" (with=" carboxylic=" or=" phosphate=" groups).=" a=" desorption=" of=" eu=" at=" ph=" above=" the=" acid=" equivalence=" point=" (ph=" 6.9)=" could=" be=" an=" indication=" of=" the=" release=" of=" a=" metal=" complexing=" exudate=" from=" the="> 相似文献
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