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Marija Marković Vladimir IvovićTijana Štajner Vitomir DjokićIvana Klun Branko BobićAleksandra Nikolić Olgica Djurković-Djaković 《Comparative immunology, microbiology and infectious diseases》2014
To contribute to the insight into the worldwide population structure of Toxoplasma gondii, we genetically characterized a total of eight strains isolated from intermediate hosts including humans, sheep and pigeons in Serbia. Although parasite DNA was detected in 28.2% (60/213) of the human samples from 162 patients serologically suspected of active toxoplasmosis, as well as in 5/7 seropositive pigeons and in 2/12 seropositive sheep examined, multilocus PCR-RFLP genotyping, using SAG1, 5′SAG2, 3′SAG2, GRA6, 5′GRA7 and 3′GRA7 as markers, was successful in only four human isolates (of which one was isolated from both the bronchoalveolar lavage fluid and blood samples of a single patient), one sheep and three pigeons. Of the eight isolates, five were type II (62.5%), one was type III, one was atypical, and one had a type I allele at GRA6 as the single locus genotyped. Although type II, as elsewhere in Europe, predominated, these results may suggest a higher genetic diversity of T. gondii in Serbia, reflecting local environmental contamination and also the geographical position of the country in South-East Europe. 相似文献
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Ivana Sedej Anamarija Mandi Marijana Saka
Aleksandra Mian Vesna Tumbas 《Cereal Chemistry》2010,87(5):387-392
Plant phenolics and tocopherols content were determined in light and wholegrain buckwheat and wheat flour. Antioxidant activity of flours were comparatively assessed by scavenging activity on 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH•), hydroxyl (•OH), and superoxide anion (O2•–) radicals, reducing activity, and chelating activity on Fe2+. Rutin, quercetin, and ferulic acid were quantified in both buckwheat flours, while ferulic acid was quantified in wholegrain wheat flour. Significantly higher content of phenolics and tocopherols was found in buckwheat than in wheat flours. Tocopherols in buckwheat flours were present in the order: γ‐ > α‐>> δ‐tocopherol, and in wheat flours: α‐ > γ‐ >> δ‐tocopherol. Buckwheat flours possessed better scavenging abilities on DPPH•, •OH and O2•– radicals, as well as better reducing activity, while wheat flours showed better chelating activity on Fe2+, according to IC50 values. Results suggest the possibility of improving the antioxidant properties of wheat‐based food products through addition of buckwheat flour. 相似文献
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Ukalska-Jaruga Aleksandra Smreczak Bożena Klimkowicz-Pawlas Agnieszka 《Journal of Soils and Sediments》2019,19(4):1890-1900
Journal of Soils and Sediments - The objective of this study was to evaluate the mutual relations between the soil organic matter (SOM) fractions: fulvic acids (FA), humic acids (HA), humins (HN),... 相似文献
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Katarzyna Makowska Sylwia Oleszczuk Aleksandra Zimny Andrzej Czaplicki Janusz Zimny 《Plant Breeding》2015,134(6):668-674
Variable efficiency of androgenesis remains a serious problem in many species of cereals. It is still unclear what makes certain genotypes more amenable to androgenesis than others. This study was undertaken to quantify the previously suspected advantage of winter barley genotypes over spring ones with regard to regeneration efficiency in anther culture. The material consisted of 40 barley hybrids originating from Polish breeding companies. The number of androgenic structures per 100 anthers did not differ significantly between analysed groups (119 vs. 152 non‐significant), but the average regeneration of green plants per 100 anthers was five times higher in winter genotypes (6.4 vs. 1.3). The incidence of albinism was lower for the winter than for the spring materials (70% vs. 90%), while the rate of spontaneous chromosome doubling was similar in both groups (58% vs. 56%). The results strongly support the notion that winter genotypes are more amenable to androgenesis and this may be a consequence of their better adaptation to stress conditions. 相似文献
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Tančić Živanov Sonja Dedić Boško Dimitrijević Aleksandra Dušanić Nenad Jocić Siniša Miklič Vladimir Kovačević Branislav Miladinović Dragana 《植物病害和植物保护杂志》2019,126(6):565-573
Journal of Plant Diseases and Protection - The study assessed the genetic variability among Macrophomina phaseolina (Tassi) Goid. isolates originating from six different countries (Turkey,... 相似文献
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Rózek A Achaerandio I Almajano MP Güell C López F Ferrando M 《Journal of agricultural and food chemistry》2007,55(13):5147-5155
Osmotic dehydration was assessed as an operation for supplementing a solid foodstuff (a gel was used as the model food) with grape phenolics from a concentrated red grape must to increase its antioxidant properties. The model food was processed for up to 24 h, and the osmotic pressure was adjusted by diluting the concentrated red grape must. In all conditions tested, low molecular weight phenolics (相似文献
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Aleksandra Maji? Agnese Marino Taussig de Bodonia ?uro Huber Nils Bunnefeld 《Biological conservation》2011,144(12):3018-3027
Successful carnivore conservation depends on public attitudes and acceptance levels of carnivores, and these are likely to change as circumstances change. Attitude studies repeated in time that can demonstrate such change are rare. Our study surveyed Croatian rural inhabitants in 2002 and in 2008 and analyzed their responses to detect a change in attitudes toward brown bears (Ursus arctos) over time. Important developments occurring in Croatia at the time of our research included a more centralized and more clearly defined bear management strategy, and an increase in the bear population. We constructed models to explain respondent’s value orientations, their level of perceived threat and their acceptance capacity for bears. Findings show that while value orientations and the overall level of perceived threat did not change over time, bear acceptance capacity was reduced. This suggests that the increase in the bear population and perhaps the more centralized bear management reduced respondents’ willingness to accept a larger bear population. We conclude that continuous public involvement in bear management is essential in order to maintain a feeling of control over the bear among the local population. Furthermore we argue that hunting is an important form of public involvement in the region, serving to reinforce existence and bequest values of the bear and increase its public acceptance. 相似文献
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Grzegorz Bartoszewski Cezary Waszczak Piotr Gawroński Iwona Stępień Hanna Bolibok-Brągoszewska Alain Palloix Véronique Lefebvre Aleksandra Korzeniewska Katarzyna Niemirowicz-Szczytt 《Euphytica》2012,186(2):453-461
The nuclear male sterility gene ms8 is expected to facilitate the production of sweet pepper (Capsicum annuum L.) hybrids as it provides means for hybridization without the labor-intensive hand emasculation of female inbred lines.
The development of molecular markers linked to ms8 locus will help the breeding practice for the selection of hybrid parental lines. In this study, F2 population resulting from a cross between the sweet pepper male sterile line 320 and the male fertile variety Elf was used
to identify DNA markers linked to the ms8 locus. With the use of RAPD–BSA technique, seven markers linked to the ms8 locus were found. Four of them were converted into SCAR markers. In addition, two COSII/CAPS markers linked to the ms8 locus were identified. Comparative mapping with reference pepper maps indicated that the ms8 locus is located on the lower arm of the pepper chromosome P4. Identified markers are useful for molecular breeding, however,
at present markers tightly linked to ms8 locus are still lacking. Identification of molecular markers linked to the ms8 locus and determination of its chromosomal localization are useful for fine mapping and also provide the perspective for
ms8 gene cloning. 相似文献