暴发性鱼病的研究及防制

暴发性鱼病是我区鱼类的常发病，主要引起白鳞、白鲢、镛、鲫、团头鲂等淡鱼类的暴发流行，通过对我区部分盟市暴发性鱼病的流行调查，分离出两株嗜水气单胞菌，经鉴定与无锡嗜水气单胞菌菌株有相同特点，并对分离菌进行致病性试验和毒素的毒性试验，分离菌具有明显的致病性和毒素毒性，并产生明显的体表出血充血以及败血症变化。对分离菌在不同的条件下培养，得知该菌可在PH6－9生长，最适温度为10－30℃，低盐或无盐状态下易于生长。将分离菌株和无锡菌株制成多价灭活全菌苗，用注射法和浸泡法对鲫鱼进行免疫，20天后攻毒，观察60天，保护率注射组为20／20，浸泡组为19／20，起到了免疫保护作用。将自制的多价灭活全菌苗应用推广于我区部分盟市，有效地防止了暴发性鱼病的发生，达到新增鲜鱼总产量129.8万公斤，新增总产值1038.4万元。提高了我区养鱼业的经济效益。     

用酶联免疫吸附试验检测蓝舌病血清抗体

我们应用农业部动物检疫所蓝舌病组提供的酶联免疫吸附试验方法检测251份山羊蓝舌病血清抗体,通过特异性试验,阻断试验利重复性试验证明其特异性和重复性较好,用本方法和琼扩对比,ELISA比琼扩的敏感性高21.5个百分点,ELISA对琼扩的     

猪传染性胸膜肺炎的诊断与防治

2006年9月,内蒙古土左旗某种猪场保育舍的猪群中突然发生以体温升高达41.5℃左右,耳、鼻、眼及后躯皮肤发绀,后期出现严重的呼吸困难和体     

9种商业消毒剂对猪2型圆环

养猪业中常用的许多商业消毒荆,都获得了法国农业部批准.然而对其杀灭增殖初期猪2型圆环病毒(PCV2)的效能却不甚了解.用在法国分离到的PCV2悬液来测试9种商业消毒剂的效能,在20℃下病毒悬液与消毒剂作用30 min后,将该混合液过滤和稀释进行脱毒处理以去除对圆环病毒和猪肾细胞系有害的物质,再经一系列稀释后接种于人工培养的猪肾细胞进行免疫过氧化物酶单层细胞试验(IPMA)测定PCV2的感染性.结果表明,用碘类和苯酚类消毒剂并不能减少病毒滴度.在此着重介绍其他消毒剂效能(病毒滴度减少量≥1.61 log10),其中5种消毒剂:一种舍过一硫酸氢钾,另两种舍一种季铵盐、一到三种醛、次氯酸钠和氢氧化钠.在标准浓度下或1.5～4倍低于标准浓度时可显著杀灭PCV2;其余两种,其中一种舍过一硫酸氢钾,另一种含过氧乙酸和过氧化氢,在标准浓度下或2倍高于标准浓度时也可杀灭PCV2.     

An analysis of an early-warning system to reduce abortions in dairy cattle in Denmark incorporating both financial and epidemiologic aspects

Emergency preparedness relies on the ability to detect patterns in rare incidents in an early stage of an outbreak in order to implement relevant actions. Early warning of an abortion storm as a result of infection with a notifiable disease, e.g. brucellosis, bovine viral diarrhea (BVD) or infectious bovine rhinotracheitis (IBR), is a significant surveillance tool. This study used data from 507 large Danish dairy herds. A modified two-stage method for detecting an unusual increase in the abortion incidence was applied to the data. An alarm was considered true if an abortion were detected in the month following the alarm month, otherwise false. The total number of abortions that could potentially be avoided if effective action were taken ranged from 769 (22.9%) to 10 (0.3%), as the number of abortions required to set the alarm increased from 1 to 6. The vast majority of abortions could, however, not be predicted, much less prevented, given this early-warning system. The false to true alarm ratio was reduced when the number of abortions that set the alarm increased. The financial scenarios evaluated demonstrated that the value of an abortion, the cost of responding to an alarm and the efficiency of the actions are important for decision making when reporting an alarm. The presented model can readily be extended to other disease problems and multiple-time periods.     

双价抗虫棉中棉所41号

~~双价抗虫棉中棉所41号@李润清$山东省菏泽市牡丹区种子管理站!274011     

油用牡丹丰产栽培综合管理技术

油用牡丹是我国一种特有的木本油用植物资源。油用牡丹产业是我国一项特色新兴产业,发展前景广阔。发展好油用牡丹产业,可产生重大的经济、社会和生态效益,助力脱贫攻坚,促进乡村振兴。近年来,我国油用牡丹种植面积不断增加。种植者正确掌握并应用相关栽培管理技术,是实现丰产增收的前提。总结介绍了油用牡丹的播种与栽植技术、苗木培育管理技术、主要病虫害及其防治技术,以期为油用牡丹的栽培管理和深入研究提供参考。     

Role of adenine nucleotide translocator 1 in mtDNA maintenance

Autosomal dominant progressive external ophthalmoplegia is a rare human disease that shows a Mendelian inheritance pattern, but is characterized by large-scale mitochondrial DNA (mtDNA) deletions. We have identified two heterozygous missense mutations in the nuclear gene encoding the heart/skeletal muscle isoform of the adenine nucleotide translocator (ANT1) in five families and one sporadic patient. The familial mutation substitutes a proline for a highly conserved alanine at position 114 in the ANT1 protein. The analogous mutation in yeast caused a respiratory defect. These results indicate that ANT has a role in mtDNA maintenance and that a mitochondrial disease can be caused by a dominant mechanism.     

Review of left-to-right shunting patent ductus arteriosus and short term outcome in 98 dogs

The case records of 98 dogs with a left-to-right shunting patent ductus arteriosus (PDA) were reviewed. There were 35 breeds represented, with a female to male ratio of 3:1. Forty per cent of the dogs were older than one year at initial presentation and 31 per cent had clinical signs attributable to PDA. A left heart base continuous murmur of grade IV/VI or higher was noted in 90 per cent of the dogs. On electrocardiography, the most common abnormalities were tall R waves (63 per cent) and deep QII waves (62 per cent). The radiographic triad of dilation of the descending aorta with enlargement of the main pulmonary artery segment and left atrium, typical of PDA, was noted in only 26 per cent of cases. Two-dimensional (2D) and M-mode echocardiography detected left atrial enlargement (35 per cent) and an increased left ventricular diameter in diastole (82 per cent) and systole (84 per cent) as the most common abnormalities. Doppler echocardiography demonstrated increased aortic outflow velocities in 66 per cent of cases. The overall short-term successful outcome in this study was 95 per cent. There was no significant difference between surgical ductal ligation using a standard technique or the Jackson-Henderson technique in terms of survival, occurrence of haemorrhage or residual shunting. The number of interventional procedures used in this study was too low for statistical comparison, but there appeared to be a trend towards a higher rate of residual shunting and a lower fatality rate using a coil occlusion technique.     

Retrieval of a patent ductus arteriosus coil following embolisation to the right subclavian artery

A two-month-old female Tibetan terrier was re-presented with an incomplete closure of the patent ductus arteriosus. Following a second attempt to close the shunt by coil embolisation, arterial embolisation occurred. The successful removal of the embolised coil is described.