The Effect of Exogenous Hormone Treatment on Spermiation in Rhynchocypris oxycephalus (Sauvage and Dabry)

For the evaluation of hormonal control of spermiation in fish, a method to quanify the spermiation response of mature Rhynchocypris oxycephalus (Sauvage and Dabry) to hormonal therapy is described. Spermatocrit was determined after 7 min centrifugation at 18,000 ± g and sperm density was estimated by a standard hemocytomer method. Sperm density can be predicted from spermatocrit since their relationship is linear as described by the regression equation, Y = 3.68X - 27.18 ( R ² = 0.82, N = 50). where Y is spermatocrit and × is sperm density. Milt production by mature R. oxycephalus was highest at 24 h after injection of 1,000 IU human chorionic gonadotropin (HCG) and 50 μg luteinizing hormone-releasing hormone analogue (LHRHa) per kg body weight. Increased milt production coincided with low spermatocrit and sperm density levels. These results demonstrate that spermiation in mature R. oxycephalus can be reliably evaluated by a spermatocrit method and that HCG and LHRHa are effective in stimulating of spermiation in this species.     

Purification and characterization of hepatic triacylglycerol lipase isolated from rainbow trout,Oncorhynchus mykiss

Trialcylglycerol (TG) lipase was isolated and partially purified from rainbow trout liver. Triacylglycerol lipase activity was assayed by measuring¹⁴C-oleic acid release from¹⁴C-triolein.¹⁴C-oleic acid release was linear for up to two hours. Optimal activity occurred at pH 7.0 and 15°C. Most of the lipase activity was recovered in the cytosolic fraction. A 27,000-fold purification was achieved after Sepharose (Bio-gel A 0.5 M, 200–400 mesh) chromatography of a resuspended 20% ammonium sulfate fraction. The molecular weight of the trout hepatic lipase as determined by size-exclusion chromatography and by SDS-polyacrylamide gel electrophoresis was 40–43 kD. Lipase-mediated hydrolysis of TG resulted in the production of diacylglycerols, monoacylglycerols, and fatty acids. Kinetic analysis indicated that V_max=0.016 nmol/h/mg protein and that K_m=0.28 mM triolein. Lipolytic activity was enhanced in the presence of cAMP/ATP-Mg²⁺. These results suggest that the liver of trout possesses a neutral TG lipase that is responsible for mobilizing stored TG and is catalytically activated by phosphorylation.A part of this work was presented at the Annual Meeting of the American Society of Zoologists, December 26–30, 1990, San Antonio, TX.     

改进芳草A系原种产卵性的试验

本试验采取母蛾延迟交配，拆对后冷藏、改变交配时间和产卵室保护温度等技术措施来探讨改善芳草A系原种产卵性的方法。结果表明，这些措施对产卵性能的改进有一定的效应，但由于性状间存在相关关系，对某一性状的促进效应往往同时也对另一产卵性状产生负向影响。     

深基坑锻锤基础冬期施工

在冬期施工，对于土方开挖、钢筋绑扎、人员的通行、砼的浇铸、砼的温度控制，基坑的保温等都带来一定困难，为此提出了深基坑锻锤基础才是最佳实践应用。     

黑杨无性系日CO2固定总量的测定及与苗木生产潜势的相关性研究

通过对不同基因型黑杨无性系植株净光合速率日变化曲线与光合主体叶面积在时间和光强上进行累计积分来计算植株日CO2固定总量P。由于净光合速率日变化曲线不易准确测得，故以光响应曲线和光强的日变化来推测净光合速率日变化。对P值和生物量的相关性研究表明，二者的相关性很高。且认为可以用计算出的P值作为评估苗木生产潜势的指标之一，这为育种中生产力的早期选择提供了有力的证据。     

粟品种对粟瘟病的抗病性初步观察

近年来,粟瘟病在山西省中部及东南部严重发生危害,輕者减产,重者籽粒不收。作者于1962—1963年进行了粟品种間抗病性的初步观察,簡报如下: 在本所試驗地和山西省晋东南区各县良种繁殖場谷子品种区域試驗地共調查品种520个。調查时期:重点品种分苗期、拔节期、拔节末期、抽穗盛期、乳熟期进行;一般品种只在发病盛期(即抽穗盛期)进行。在自然条件下未感染粟瘟病的品种,再在溫室中种植,进行人工接种。抗病性鑑定結果如下: (一)尚未发現有免疫品种。 (二)不同品种对栗瘟病的抗病性有显著差异:在发病盛期調查,高度抗病品种61个,占总数12％,发病指数为0.1—10;抗病品种149个,占总数28.7％,发病指数为10.1—20.0;輕度感病品种128个,占总数24.6％;发病指数为20.1—     

如何在和谐社会中培养优秀的音乐教育人才

未来社会需要高素质的、德才兼备的、具有宽广知识、一专多能的音乐教育人才.在提高专业技能知识的同时,培养学生综合素质,是当前和谐社会中培养高素质人才的关键.     

上海水牛生理生化指标的测定和分析

为研究上海水牛生理生化指标含量,选取精神良好、健康的上海水牛犊牛(1岁内)、青年牛(1-2岁)、成年牛(3岁以上),测定了生理指标、血液生理生化指标含量。结果显示,上海水牛的体重随月龄的增加显著增加(P＜0.05),而呼吸频率、心率无显著差异(P＞0.05)。在检测的22项血液生理指标含量中,有10项指标(平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、红细胞分布宽度(RDW)、淋巴细胞百分比(%LYM)、单核细胞百分比(%MONO)、中性粒细胞百分比(%NEU)、嗜酸性粒细胞百分比(%EOS)、淋巴细胞(LYM)、单核细胞(MONO))含量差异显著(P＜0.05),其余12项指标(红细胞(RBC)、红细胞比容(HCT)、血红蛋白(HGB)、网织红细胞百分比(%RETIC)、网织红细胞(RETIC)、白细胞(WBC)、嗜碱性粒细胞百分比(%BASO)、中性粒细胞(NEU)、嗜酸性粒细胞(EOS)、嗜碱性粒细胞(BASO)、血小板(PLT)、血小板压积(PCT))含量差异不显著(P＞0.05)。在检测的18项血液生化指标含量中,有9项指标(血糖(GLU)、肌酐(CREA)、磷离子(PHOS)、钙离子(Ca)、总蛋白(TP)、球蛋白(GLOB)、丙氨酸转氨酶(ALT)、胆固醇(CHOL)、胰淀粉酶(AMYL))含量差异显著(P＜0.05),其余9项指标(尿素(UREA)、血尿素氮/肌酐比(BUN/CREA)、白蛋白(ALB)、白蛋白/球蛋白比(ALB/GLOB)、碱性磷酸酶(ALKP)、谷氨酰转移酶(GGT)、总胆红素(TBIL)、甘油三酯(TRIG)、脂肪酶(LIPA))含量差异不显著(P＞0.05)。该研究可为上海水牛的选种选育、生长发育评估、营养状况评估、疾病诊断等提供参考。     

城市夜间形象初探

从城市规划和建筑学的角度研究城市夜间形象;结合形态美学、行为心理学、环境心理学等学科进行研究,将安全感、视觉舒适性、视觉趣味、活动激发性以及可识别性作为夜间形象评价指标并分别进行具体地分析。