Studies on Tomato Seedling Quality Indices Under Simulated Shipping and Storage Conditions

Indices of the tomato seedling quality maintenance level after production before field planting were studied through simulated experiments, small--scale operation, indoor analyses and measurements, and field observation. The results showed that under simulated shipping and storage conditions, seedling quality change following different durations (days) of shipping and storage was correlated significantly or even very significantly with certain physiological and morphological indices. With various measured indices following different periods of shipping and storage treatment subjected to multinomial successive regressive correlation analysis, the principal factors influencing seedling quality maintenance level are identified to be chlorophyll content→dry short weight→ leaf freshness index in order of their importance. Significance analysis with multinomial fitted equation indicated that correlations between any one of above three factors and the growth index after field planting reached very significant difference level.     

棉花不同时期去叶源对库源关系影响的初步研究

通过对棉花不同时期去主茎叶、叶枝叶、果枝叶和全部叶片的研究,初步探讨了棉花叶源对库的影响.结果表明:无论蕾期还是盛铃期,叶片是棉花光合产物的主要来源,没有叶片棉花就不能正常生长,也很难保证其产量;不同部位的叶片中,主茎叶的作用显著,蕾期去主茎叶后株高日增长量减少0.5 cm以上,干物质积累明显下降,盛铃期则导致棉铃大量脱落,成铃平均减少2个以上;去果枝叶的作用相对较小,去叶枝叶几乎无影响.     

鸭大肠杆菌病流行病学与病原特性研究

对浙江等地鸭大肠杆菌病流行病学进行调查，并研究其发病特点，分离鉴定出致病性大肠杆菌26株；已对其中20株菌株进行血清型鉴定，主要为078，占70%（14/20），其次是O1，占15%（3/20）；人工感染试验表明，80%（8/10）菌株毒力强，皮下、肌肉、足蹼3种途径均易复制该病；病理组织学检查肝、肺、脾等，表现急性炎症，出血等病变；药敏试验表明，不同菌株对药物敏感性差异较大，除对鸭菌消、卡那霉素等高敏外，对多数常用抗菌药已耐药，最后就该病的防治提出了对策。     

Effects of Taoren Honghua Jian on the hyperlipidemia without symptom

AIM: To examine the effect of traditional chinese medicine recipe, Taoren Honghua(semen persicae-flos carthami) decoction, on hyperlipidemia without symptom. METHODS: The plasma TC, TG, LDL, HDL, apolipoprotein(Apo) A, Apo Bof the patients with hyperlipidemia without symptom were measured using automatic analyzer (shimadiu CL-7200), the production of nitric oxide(NO) was detected by Greiss reaction, and SODactivity and MDAformation were examined using o-trihydroxy benzene and barbituric methotheds, respectively. RESULTS: After oral administration of Taoren Honghua decoction, the plasma levels of TC, TG, LDL, and MDAof the patients were markedly decreased, however, the plasma levels of ApoA, HDL, SODand NOwere significantly increased and almost no change was detected in the plasma level of Apo B. In control group, it was found that although the plasma level of TC, TG and LDL were decreased ( P<0.05 ) and ApoAas well as HDLwere increased, ApoB, SOD, MDA and NO production were all unchanged. CONCLUSION: The traditional chinese medicine recipe, Taoren Honghua decoction,has a significant therapeutic effect on patients with hyperlipidemia by removing blood stasis, promoting qi circulation and in turn reducing blood lipid level.     

商品肉猪胴体性状的测定及其相关性分析

引进丹麦先进仪器FAT—O—MEAT’ER(肉脂仪)对中国鲁梅克斯食品总公司名山县屠宰场中5821头猪进行了现场测定，测量性状为最后一根肋骨处离背中线4～5cm处的膘厚(P2)和倒数第三、四根肋骨之间，离背中线4—5cm处的膘厚(RF)以及眼肌厚度(RM)，并估测出瘦肉率。对所测胴体各性状的均数和相关性分析，表明本地商品肉猪胴体质量低于国外水平。     

结核分枝杆菌分泌蛋白ESAT-6、CFP10基因的克隆、鉴定及其原核表达

以人型结核分枝杆菌 H37RV株基因组 DNA为模板 ,应用 PCR法对 ESAT- 6和 CFP10基因进行扩增 ,产物经纯化后与载体 PMD18- T连接、转化及酶切鉴定 ,亚克隆到原核表达载体 PGEX- 6 P- 1,构建原核重组表达质粒 ,转化入大肠杆菌 BL2 1中 ,以 1mmol/L IPTG诱导 ,进行 SDS- PAGE电泳。结果表明 ,ESAT- 6和 CFP10基因表达的融合蛋白相对分子质量分别为 32 0 0 0和 36 0 0 0 ,与实测相符。重组结核杆菌分泌蛋白 ESAT- 6和 CFP10的成功表达为结核病诊断及重组疫苗的构建打下了基础     

猪胸膜肺炎放线杆菌外膜蛋白(OMP)5′末端保守区基因的克隆、表达及其免疫活性研究

以猪胸膜肺炎放线杆菌血清7型25-4株基因组DNA为模板,PCR方法扩增外膜蛋白(OMP)5′末端保守区基因片段(OMPc),酶切及核苷酸序列分析鉴定后,与原核表达载体质粒pGEX-6P-1进行连接,构建成重组表达载体pGEX-OM-Pc,转入大肠杆菌BL21中,以IPTG进行诱导,SDS-PAGE电泳分析发现,转化了重组质粒的菌株所表达的融合蛋白相对分子量为34 kD,与实际预测相符,命名为GST-OMPc.GST亲和层析柱进行纯化,ELISA方法对纯化蛋白进行检测.结果表明:纯化蛋白GST-OMPc能够与兔抗猪胸膜肺炎放线杆菌血清7型的阳性血清反应.OMPc蛋白的成功表达为其功能的研究打下基础.     

鸭疫里氏杆菌形态特征的电镜观察

自鸭传染性浆膜炎的病死鸭中分离鉴定的鸭疫里氏杆菌(RA)Ⅰ、Ⅱ型两个菌株，用负染法和超薄切片法制备样品，于透射电镜下进行观察。结果观察到约占1／3的RA具有特殊的形态结构：在细菌的顶端或侧面有1～2个与菌体相连的芽状赘生物，有的也能从菌体上脱落下来。它在超薄切片中的大小约为菌体的1／3～1／5，能观察到其内部也具有类似菌体内部的结构。且具有类似菌体的“细胞壁”，类似于核体部分的结构位于“细胞壁”的一侧。而用相同的方法制作的鸭大肠杆菌(E．cozi)对照样品，却见不到这种特殊的形态结构。说明这种形态特征可能是RA特有的。     

6种人兽共患病病原核酸液相芯片检测体系的建立

本研究通过设计、合成特异性引物和探针并对其进行修饰,将探针和特定荧光编码微球共价交联后,利用Luminex检测仪读取荧光信号,从而建立了针对布鲁氏菌、沙门氏菌、弓形虫,狂犬病病毒、巴氏通体、弯曲杆菌等6种病原的液相芯片检测方法。数据显示,本研究建立的液相芯片检测体系具有良好的特异性,检测灵敏度达50拷贝数,能同时检测6种重要人兽共患病病原,对于这6种人兽共患病的快速筛查和控制提供了技术支撑。     

池塘和稻田养殖模式下克氏原螯虾肌肉和肝脏营养成分比较

通过对池塘和稻田2种养殖模式下克氏原螯虾(Procambarus clarkii)的肌肉常规营养成分进行测定,同时,对肌肉和肝胰腺的氨基酸和脂肪酸组成进行测定,从而对其营养品质进行分析评价.结果显示,2种养殖模式下克氏原螯虾粗蛋白和粗脂肪无显著性差异(P＞0.05),池塘养殖模式下水分显著高于稻田模式(P＜0.05),...