全文获取类型
收费全文 | 1305篇 |
免费 | 78篇 |
专业分类
林业 | 91篇 |
农学 | 35篇 |
基础科学 | 3篇 |
253篇 | |
综合类 | 123篇 |
农作物 | 48篇 |
水产渔业 | 82篇 |
畜牧兽医 | 677篇 |
园艺 | 15篇 |
植物保护 | 56篇 |
出版年
2023年 | 7篇 |
2022年 | 12篇 |
2021年 | 31篇 |
2020年 | 33篇 |
2019年 | 36篇 |
2018年 | 41篇 |
2017年 | 37篇 |
2016年 | 36篇 |
2015年 | 37篇 |
2014年 | 45篇 |
2013年 | 76篇 |
2012年 | 84篇 |
2011年 | 91篇 |
2010年 | 52篇 |
2009年 | 42篇 |
2008年 | 72篇 |
2007年 | 65篇 |
2006年 | 78篇 |
2005年 | 60篇 |
2004年 | 59篇 |
2003年 | 34篇 |
2002年 | 38篇 |
2001年 | 28篇 |
2000年 | 16篇 |
1999年 | 13篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 8篇 |
1995年 | 9篇 |
1992年 | 14篇 |
1991年 | 8篇 |
1990年 | 13篇 |
1989年 | 10篇 |
1988年 | 18篇 |
1987年 | 5篇 |
1986年 | 13篇 |
1985年 | 4篇 |
1984年 | 13篇 |
1983年 | 9篇 |
1979年 | 8篇 |
1977年 | 4篇 |
1971年 | 4篇 |
1970年 | 8篇 |
1967年 | 5篇 |
1966年 | 7篇 |
1933年 | 4篇 |
1929年 | 6篇 |
1914年 | 3篇 |
1905年 | 5篇 |
1904年 | 3篇 |
排序方式: 共有1383条查询结果,搜索用时 15 毫秒
1.
Biological, physico-chemical and immunochemical methods are available today for solving the multiple problems which turn up in the course of mycotoxin diagnosis and mycotoxin research. In this review, selected analysis techniques are presented and their limits and possibilities are discussed. The biological methods (chicken-embryo-test, skin irritation test, cell cultures) indicate only toxicities, mostly without further specification for mycotoxins. In contrast, modern physico-chemical analysis (DC, HPLC, CLC, MS) allows the detection of defined mycotoxins in the picogram range; these methods should be employed or official and judicial purposes. The recently developed immunochemical methods (RIA, EIA) are characterized by a simple sample preparation, a short test duration and a high sensitivity; they could be used as a screening method in the course of mycotoxicological control of food and feed. 相似文献
2.
Evaluation of reticulocyte hemoglobin content (RET‐He) in the diagnosis of iron‐deficient erythropoiesis in dogs 下载免费PDF全文
Jannika Fuchs Andreas Moritz Esther Grußendorf Jörg Lechner Felix Neuerer Rafael Nickel Thomas Rieker Claudia Schwedes Dennis B. DeNicola James Russell Natali Bauer 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(4):558-568
Background
Reticulocyte hemoglobin content provided by the Siemens ADVIA (CHr) is an established marker of iron deficiency. The IDEXX ProCyte Dx hematology analyzer now provides a similar variable, reticulocyte hemoglobin equivalent (RET‐He).Objectives
The objective was to evaluate RET‐He and its diagnostic utility in dogs, and to calculate a cutoff value for diagnosing iron‐deficient erythropoiesis (IDE). Furthermore, the prevalence of RET‐He values below this cutoff value was established.Methods
One hundred and seventy‐one CBCs of healthy dogs were used to establish a RI. Stability of RET‐He was evaluated by repeated measurements over 48 hours (n = 10). The 25‐run coefficient of variation (CV) was calculated, and correlation and bias between measurements of RET‐He and CHr were assessed (n = 190). A cutoff value for diagnosing IDE was calculated. The utility of RET‐He in the detection of IDE was evaluated in 123 dogs. The prevalence of low RET‐He values was assessed retrospectively in a multicenter study (2012–2014) under participation of 7 veterinary clinics.Results
Reticulocyte hemoglobin equivalent with an RI of 22.2 to 28.6 pg was statistically stable over 48 hours (P = .10). The CV was 1.8%. A fair correlation (ρ = 0.74) between RET‐He and CHr with a small bias of ?0.6 pg was found. The cutoff value for diagnosing IDE was 20.9 pg (sensitivity: 85%; specificity: 99%). The prevalence of RET‐He values below 20.9 pg was 10.3% (1084/10,553 dogs).Conclusions
RET‐He on the ProCyte Dx is a precise screening tool in dogs to detect iron‐deficient erythropoiesis. 相似文献3.
Eva van Gorsel Nicolas Delpierre Ray Leuning Andy Black J. William Munger Steven Wofsy Marc Aubinet Christian Feigenwinter Jason Beringer Damien Bonal Baozhang Chen Jiquan Chen Robert Clement Kenneth J. Davis Ankur R. Desai Danilo Dragoni Sophia Etzold Thomas Grünwald Lianhong Gu Bernhard Heinesch Lucy R. Hutyra Wilma W.P. Jans Werner Kutsch B.E. Law Monique Y. Leclerc Ivan Mammarella Leonardo Montagnani Asko Noormets Corinna Rebmann Sonia Wharton 《Agricultural and Forest Meteorology》2009,149(11):1919
Micrometeorological measurements of nighttime ecosystem respiration can be systematically biased when stable atmospheric conditions lead to drainage flows associated with decoupling of air flow above and within plant canopies. The associated horizontal and vertical advective fluxes cannot be measured using instrumentation on the single towers typically used at micrometeorological sites. A common approach to minimize bias is to use a threshold in friction velocity, u*, to exclude periods when advection is assumed to be important, but this is problematic in situations when in-canopy flows are decoupled from the flow above. Using data from 25 flux stations in a wide variety of forest ecosystems globally, we examine the generality of a novel approach to estimating nocturnal respiration developed by van Gorsel et al. (van Gorsel, E., Leuning, R., Cleugh, H.A., Keith, H., Suni, T., 2007. Nocturnal carbon efflux: reconciliation of eddy covariance and chamber measurements using an alternative to the u*-threshold filtering technique. Tellus 59B, 397–403, Tellus, 59B, 307-403). The approach is based on the assumption that advection is small relative to the vertical turbulent flux (FC) and change in storage (FS) of CO2 in the few hours after sundown. The sum of FC and FS reach a maximum during this period which is used to derive a temperature response function for ecosystem respiration. Measured hourly soil temperatures are then used with this function to estimate respiration RRmax. The new approach yielded excellent agreement with (1) independent measurements using respiration chambers, (2) with estimates using ecosystem light-response curves of Fc + Fs extrapolated to zero light, RLRC, and (3) with a detailed process-based forest ecosystem model, Rcast. At most sites respiration rates estimated using the u*-filter, Rust, were smaller than RRmax and RLRC. Agreement of our approach with independent measurements indicates that RRmax provides an excellent estimate of nighttime ecosystem respiration. 相似文献
4.
5.
Sanloup C Schmidt BC Chamorro Perez EM Jambon A Gregoryanz E Mezouar M 《Science (New York, N.Y.)》2005,310(5751):1174-1177
The reactivity of xenon with terrestrial oxides was investigated by in situ synchrotron x-ray diffraction. At high temperature (T > 500 kelvin), some silicon was reduced, and the pressure stability of quartz was expanded, attesting to the substitution of some xenon for silicon. When the quartz was quenched, xenon diffused out and only a few weight percent remained trapped in samples. These results show that xenon can be covalently bonded to oxygen in quartz in the lower continental crust, providing an answer to the missing xenon problem; synthesis paths of rare gas compounds are also opened. 相似文献
6.
Scott HM Sorensen O Wu JT Chow EY Manninen K 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(4):397-406
A province-wide cross-sectional seroprevalence and agroecological risk factor study of Mycobacterium avium subspecies paratuberculosis (MAP) and Neospora caninum (NC) infection among cattle in 100 cow-calf herds in Alberta was conducted. The seroprevalence of MAP in adult cattle was 1.5% across all herds. Using a widely accepted herd test cutpoint of 2 or more seropositive cows out of 30 animals tested, 7.9% of herds were estimated to be infected (95% confidence interval (CI): 2.3-23.4%). Seroprevalence of MAP differed by agroecological region; specifically, cattle and herds in areas with high soil pH (> 7.0), southern latitudes, and arid climates had a moderately reduced risk of infection (P < 0.10). Seroprevalence of NC infection was 9.7% among adult beef cattle province-wide--these levels also varied by agroecological region--with 91.0% of herds infected overall. 相似文献
7.
Pastalkova E Serrano P Pinkhasova D Wallace E Fenton AA Sacktor TC 《Science (New York, N.Y.)》2006,313(5790):1141-1144
Analogous to learning and memory storage, long-term potentiation (LTP) is divided into induction and maintenance phases. Testing the hypothesis that the mechanism of LTP maintenance stores information requires reversing this mechanism in vivo and finding out whether long-term stored information is lost. This was not previously possible. Recently however, persistent phosphorylation by the atypical protein kinase C isoform, protein kinase Mzeta (PKMz), has been found to maintain late LTP in hippocampal slices. Here we show that a cell-permeable PKMz inhibitor, injected in the rat hippocampus, both reverses LTP maintenance in vivo and produces persistent loss of 1-day-old spatial information. Thus, the mechanism maintaining LTP sustains spatial memory. 相似文献
8.
Vermeire S Audenaert K De Meester R Vandermeulen E Waelbers T De Spiegeleer B Eersels J Dobbeleir A Peremans K 《Research in veterinary science》2011,91(3):465-472
The serotonergic system is disturbed in different mood and affective disorders, with especially the serotonin (5-HT) 2A receptor involved in impulsive aggressiveness and anxiety. The aim of the study was to evaluate the involvement of the brain 5-HT 2A receptor in dogs with different behavioural disorders. Three groups of drug naive dogs were studied: 22 dogs showing impulsive aggressive behaviour, 22 showing normal behaviour, and 22 showing anxious behaviour. The serotonin 2A receptor was evaluated with Single Photon Emission Computed Tomography (SPECT) and the serotonin 2A receptor-selective radiopharmaceutical (123)I-R91150. A serotonin 2A receptor binding index (BI), proportional to the cortical receptor density, was calculated. A receiver operating characteristic (ROC) analysis was performed to determine cut-off values at which optimal sensitivity and specificity are achieved and to evaluate the general performance of the BI in reflecting the state of the dog, i.e., impulsive aggressive, normal or anxious. Significantly (P<0.0056) altered 5-HT 2A receptor binding indices were found in bilateral frontal, temporal and occipital cortical brain areas of the dogs behaving abnormally, with consistently increased BI in impulsive aggressive dogs and decreased BI in anxious dogs. These results provide clear evidence for a disturbed serotonergic balance in canine impulsive aggression and anxiety disorders. A right frontal cut-off value of ≥1.92 with 86.4% sensitivity and 2.3% (1-specificity) was obtained for the impulsive aggressive dogs. Differentiating the anxious dogs from the rest of the population was possible with a cut-off value of ≤1.73 with 86.4% sensitivity and 18.2% (1-specificity). We conclude that SPECT imaging with the radioligand (123)I-R91150 can be a helpful tool in evaluating the involvement of the serotonin 2A receptor in the complex mechanisms of impulsive aggressive and anxious behaviour. The 5HT-2A binding index of the right frontal cortex appears to be a valid biomarker in differentiating the studied canine behavioural disorders. 相似文献
9.
10.
Makenzie Kerr Jeremy Browning Eva‐Maria Bnnelycke Yingjun Zhang Chuanmin Hu Maickel Armenteros Steven Murawski Ernst Peebles Mya Breitbart 《Fisheries Oceanography》2020,29(4):340-348
Identifying spawning sites for broadcast spawning fish species is a key element of delineating critical habitat for managing and regulating marine fisheries. Genetic barcoding has enabled accurate taxonomic identification of individual fish eggs, overcoming limitations of morphological classification techniques. In this study, planktonic fish eggs were collected at 23 stations along the northwestern coast of Cuba and across the Florida Straits to United States waters. A total of 564 fish eggs were successfully identified to 89 taxa within 30 families, with the majority of taxa resolved to species. We provide new spawning information for Luvarus imperialis (Louvar), Bothus lunatus (Plate Fish), Eumegistus illustris (Brilliant Pomfret), and many economically important species. Data from most sites supported previously established patterns of eggs from neritic fish species being found on continental shelves and oceanic species spawning over deeper waters. However, some sites deviated from this pattern, with eggs from reef‐associated fish species detected in the deep waters of the Florida Straits and pelagic species detected in the shallow, continental shelf waters off the coast of northwestern Cuba. Further investigation using satellite imagery revealed the presence of a mesoscale cyclonic eddy that likely entrained neritic fish eggs and transported them into the Florida Straits. The technique of combining DNA‐based fish egg identification with remotely‐sensed hydrodynamics provides an important new tool for assessing the interplay of regional oceanography with fish spawning strategies. 相似文献