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1.
Soo-Young CHOI Woo-Chang JEONG Young-Won LEE Ho-Jung CHOI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):239-244
Contrast enhanced ultrasound (CEUS) is useful to evaluate tissue perfusion in the kidney. In veterinary
medicine, sedation or anesthesia may be required in uncooperative or panting patients. The aim of this study
was to evaluate and compare the normal kidney perfusion patterns in conscious and anesthetized dogs using
CEUS. Eight healthy beagles were used in this study. Scanning was performed in conscious dogs using manual
restraint (conscious group), or under general anesthesia using tiletamine-zolazepam and medetomidine (TZM
group) or medetomidine (M group). The contrast agent (Sonovue®) was administered as an IV bolus.
The peak intensity (PI), time to peak enhancement from injection (TTP0) and the time to peak
enhancement from the initial rise (TTPup), upslope, downslope and area under the curve (AUC) were
analyzed. Compared to the cortical values in the conscious group, TTP0 was significantly delayed in
the TZM group, and upslope, TTP0 and TTPup were significantly different in the M group.
The AUCs in the TZM and M groups were not different from those in the conscious group. The upslope of renal
medullary perfusion was significantly decreased in the TZM and M groups. TTP0 and TTPup
were also significantly delayed in these groups. The AUC of the medulla was significantly decreased in the M
group. Therefore, TZM is useful as an anesthetic protocol when performing CEUS, and the obtained data may
serve as reference values in the evaluation of renal perfusion using CEUS in dogs under anesthesia. 相似文献
2.
Hyunkyoung LEE Hyeshin HWANG Younghye RO Ji-Hyeon KIM Kyunghyun LEE Eunjin CHOI Youchan BAE Byungjae SO Inhyung LEE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):869
This study was performed to examine and clarify the cause of hindlimb ataxia and neuropathy seen in the South Korean horse population. Fifty horses diagnosed with hindlimb ataxia and neuropathy were referred for this study. Neurological examination was performed on 47 horses while necropsy was performed in all 50 animals. The occurrence of neurological diseases increased rapidly in the summer and 47 out of 50 horses were referred after the end of July. The incidence of neurological diseases started from the southern part of Korea in July and proceeded northward in August and September. Although there was no correlation with age, Thoroughbred and Warmblood horses showed a higher incidence rate than Halla and Jeju horses. The incidence rate was 5 times higher in geldings than in mares and stallions. Of the 20 cases, 16 were diagnosed with eosinophilic meningoencephalomyelitis in 2015. The most common lesions observed in 2016 were parasitic meningoencephalomyelitis (10 cases, 33%) and eosinophilic meningomyelitis (7 cases, 23%). Histopathological analysis of the brain and spinal cord revealed nematodes of approximately 100–200 µm in diameter, microcavitation and infiltrates of eosinophils, and brown pigmented macrophage infiltrates. The nematodes were identified as Setaria digitata via DNA sequencing, performed subsequent to polymerase chain reaction using DNA isolated from formalin-fixed paraffin-embedded tissue sections of the spinal cord. These results show that aberrant migration of Setaria digitata larva in the brain and spinal cord was a major cause for neurological signs in horses. 相似文献
3.
链霉菌FR-008产生一种七烯大环内酯类抗生素FR-008,对真菌具有很高的抗菌活性,并对蚊子幼虫有高毒性(袁德军和周启,1990)。负责合成FR-008的基因簇被定位(Hu et al.,1994),有21个基因(共138kb,GenBank accession number AY310323)被确认参与了抗生素FR-008的生物合成以及调节和外运(Chen et al.,2003)。然而,在FR-008基因簇最左端fscO基因上游的基因是否与FR-008抗生素生物合成相关?为此,本研究对FR-008基因簇上游区域进行了序列测定和分析。 相似文献
4.
Bo-Seong SEO Young-Jae JEONG Nu-Ri BAEK Hyun-Jin PARK Hye In YANG Se-In PARK Woo-Jung CHOI 《土壤圈》2022,32(6):905-915
Electrical conductivity(EC) of soil-water extracts is commonly used to assess soil salinity. However, its conversion to the EC of saturated soil paste extracts(ECe), the standard measure of soil salinity, is currently required for practical applications. Although many regression models can be used to obtain ECe from the EC of soil-water extracts, the application of a site-specific model to different sites is not straightforward due to confounding soil factors such as soil texture. This study was... 相似文献
5.
JEONGMIN LEE KIDONG EOM YUNSANG SEONG HAEOON LEE JINHEE PARK JONGWON LEE KWANGHO JANG KEUNWOO LEE TAEHO OH SANGGWAN LEE JUNGHEE YOON HEECHUN LEE HOJUNG CHOI YOUNWON LEE DONGWOO CHANG 《Veterinary radiology & ultrasound》2006,47(1):94-98
Ultrasonographic imaging of the canine external ear canal, tympanic membrane, and tympanic bulla was described in five healthy beagle dogs before and after infusion of saline into the ear canal. Saline served as an acoustic window. With this method, the external ear canal, and tympanic bulla were visible in the same imaging plane and the integrity of the tympanic membrane could be evaluated indirectly by confirming an intact tympanic membrane, which appeared at the end of the ear canal as a hyperechoic line with reverberation. Experimentally, perforated tympanic membrane could be evaluated by identifying anechoic saline in the tympanic bulla lumen. The air and fluid-filled tympanic bulla were also visualized. Ultrasonography with saline as an acoustic window appears to be helpful for the evaluation of the external ear canal, tympanic membrane, and tympanic bulla and it may have the potential to be a useful clinical tool in evaluation of integrity of the tympanic membrane. 相似文献
6.
Frederick J. Muehlbauer Seungho Cho Ashutosh Sarker Kevin E. McPhee Clarice J. Coyne P. N. Rajesh Rebecca Ford 《Euphytica》2006,147(1-2):149-165
Summary Lentil is a self-pollinating diploid (2n = 14 chromosomes) annual cool season legume crop that is produced throughout the world and is highly valued as a high protein
food. Several abiotic stresses are important to lentil yields world wide and include drought, heat, salt susceptibility and
iron deficiency. The biotic stresses are numerous and include: susceptibility to Ascochyta blight, caused by Ascochyta lentis; Anthracnose, caused by
Colletotrichum truncatum; Fusarium wilt, caused by
Fusarium oxysporum; Sclerotinia white mold, caused by
Sclerotinia sclerotiorum; rust, caused by
Uromyces fabae; and numerous aphid transmitted viruses. Lentil is also highly susceptible to several species of
Orabanche prevalent in the Mediterranean region, for which there does not appear to be much resistance in the germplasm. Plant breeders
and geneticists have addressed these stresses by identifying resistant/tolerant germplasm, determining the genetics involved
and the genetic map positions of the resistant genes. To this end progress has been made in mapping the lentil genome and
several genetic maps are available that eventually will lead to the development of a consensus map for lentil. Marker density
has been limited in the published genetic maps and there is a distinct lack of co-dominant markers that would facilitate comparisons
of the available genetic maps and efficient identification of markers closely linked to genes of interest. Molecular breeding
of lentil for disease resistance genes using marker assisted selection, particularly for resistance to Ascochyta blight and
Anthracnose, is underway in Australia and Canada and promising results have been obtained. Comparative genomics and synteny
analyses with closely related legumes promises to further advance the knowledge of the lentil genome and provide lentil breeders
with additional genes and selectable markers for use in marker assisted selection. Genomic tools such as macro and micro arrays,
reverse genetics and genetic transformation are emerging technologies that may eventually be available for use in lentil crop
improvement. 相似文献
7.
Soo-Young CHOI Ho-Jung CHOI Ki-Ja LEE Young-Won LEE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1049-1054
To establish a protocol for a multi-phase computed tomography (CT) of the
canine pancreas using the bolus-tracking technique, dynamic scan and multi-phase CT were
performed in six normal beagle dogs. The dynamic scan was performed for 60 sec at 1-sec
intervals after the injection (4 ml/sec) of a contrast medium, and
intervals from aortic enhancement appearance to aortic, pancreatic parenchymal and portal
vein peaks were measured. The multi-phase CT with 3 phases was performed three times using
a bolus-tracking technique. Scan delays were 0, 15 and 30 in first multi-phase scan; 5, 20
and 35 in second multi-phase scan; and 10, 25 and 40 sec in third multi-phase scan,
respectively. Attenuation values and contrast enhancement pattern were analyzed from the
aorta, pancreas and portal vein. The intervals from aortic enhancement appearance to
aortic, pancreatic parenchymal and portal vein peaks were 3.8 ± 0.7, 8.7 ± 0.9 and 13.3 ±
1.5 sec, respectively. The maximum attenuation values of the aorta, pancreatic parenchyma
and portal vein were present at scan sections with no scan delay, a 5-sec delay and a
10-sec delay, respectively. When a multi-phase CT of the canine pancreas is triggered at
aortic enhancement appearance using a bolus-tracking technique, the recommended optimal
delay times of the arterial and pancreatic parenchymal phases are no scan delay and 5 sec,
respectively. 相似文献
8.
Jin-Gu NO Mi-Kyung CHOI Dae-Jin KWON Jae Gyu YOO Byoung-Chul YANG Jin-Ki PARK Dong-Hoon KIM 《The Journal of reproduction and development》2015,61(2):90-98
Pretreatment of somatic cells with undifferentiated cell extracts, such as embryonic stem cells and mammalian oocytes, is an attractive alternative method for reprogramming control. The properties of induced pluripotent stem cells (iPSCs) are similar to those of embryonic stem cells; however, no studies have reported somatic cell nuclear reprogramming using iPSC extracts. Therefore, this study aimed to evaluate the effects of porcine iPSC extracts treatment on porcine ear fibroblasts and early development of porcine cloned embryos produced from porcine ear skin fibroblasts pretreated with the porcine iPSC extracts. The ChariotTM reagent system was used to deliver the iPSC extracts into cultured porcine ear skin fibroblasts. The iPSC extracts-treated cells (iPSC-treated cells) were cultured for 3 days and used for analyzing histone modification and somatic cell nuclear transfer. Compared to the results for nontreated cells, the trimethylation status of histone H3
lysine residue 9 (H3K9) in the iPSC-treated cells significantly decreased. The expression of Jmjd2b, the H3K9 trimethylation-specific demethylase gene, significantly increased in the iPSC-treated cells; conversely, the expression of the proapoptotic genes, Bax and p53, significantly decreased. When the iPSC-treated cells were transferred into enucleated porcine oocytes, no differences were observed in blastocyst development and total cell number in blastocysts compared with the results for control cells. However, H3K9 trimethylation of pronuclear-stage-cloned embryos significantly decreased in the iPSC-treated cells. Additionally, Bax and p53 gene expression in the blastocysts was significantly lower in iPSC-treated cells than in control cells. To our knowledge, this study is the first to show that an extracts of porcine iPSCs can affect histone modification and gene expression in porcine ear
skin fibroblasts and cloned embryos. 相似文献
9.
10.
Owing to unique structure and characteristics of carbon nanotubes, much more are attracted attentions since they were discovered. In recent years, carbon nanotubes have been successfully synthesized by various methods. Especially, highly aligned carbon nanotubes were grown by chemical vapor deposition (CVD) and the oriented growth of carbon nanotubes became realized, resulting in many applications of carbon nanotubes and researches on them. In the work, the methods and mechanisms of orientation growth of carbon nanotubes were reviewed, and it was analyzed and discussed that different methods influenced on the process of growing carbon nanotubes. Simultaneously, it was emphatically analyzed that catalyst particles affected on orientation growth of carbon nanotubes during their growth. 相似文献