饥饿、季节及性别对大鲵肌肉、肝脏生化成分的影响

季节会影响到大鲵肌肉的脂肪及钙的含量,肝脏的脂肪、钙及游离氨基酸的含量,饥饿对肌肉的酸化成分不产生影响,但饥饿程度的增加,会对肝脏脂肪无氮浸出物及游离氨基酸产生影响;繁殖前雌性大鲵的肌肉肝脏的肪肪含量均高于雄性的。     

Association of tumor-associated antigen on the proliferation of bovine leukemia virus-infected lymphoblastoid B-cell lines.

The association of tumor-associated antigen (TAA) on the proliferation of BLV-infected lymphoblastoid B-cell lines (BL2M3 and BL312) was investigated. Flow cytometric analysis of the expression of TAA with monoclonal antibody (mAb) c143 showed high expression of TAA on the surfaces of BL2M3 and BL312 cells. A large amount of TAA was found in the culture supernatant of BL2M3 and BL312 cells as well as in the lysates of BL2M3 and BL312 cells. Culture supernatant but not lysates of BL2M3 and BL312 cells promoted the growth of either BL2M3 cells or BL312 cells. Furthermore, this growth promoting activity in culture supernatants of BL2M3 and BL312 cells was inhibited in a dose-dependent manner when cultured with mAb c143. These results suggested that TAA may be involved in the growth factor-mediated cell growth of bovine B-lymphoblastoid cell lines expressing TAA on their cell surface.     

干巴菌（Thelephoraganbajun Zang）的研究Ⅰ．干巴菌与其生境因子的关系

本文研究了干巴菌的分布及其在分布区内的植被类型，查清了干巴菌主要分布于针叶树林中，尤以云南松林内分布最多，但也分布于纯的栗树林中。通过对于巴菌生境中土壤微生物的分析，结果与宏观调查一致：其生长土壤较为贫瘠，是一种菌根菌。     

Seroprevalence of porcine and human influenza A virus antibodies in pigs between 1986 and 1988 in Hassia

1,268 sera collected from slaughtered pigs in Hassia (FRG) from 1986 to 1988 were tested for antibodies against porcine and human influenza A virus strains using the single radial haemolysis test (SRHT). Antibodies against the porcine strains (subtype H1N1) A/Swine/Arnsberg/1/81, A/Swine/Iowa/15/30 and A/New Jersey/7/76 were detected in 411 (32.4%), 318 (25.1%) and 304 (24.0%) of sera, respectively. Up to 1988 a slight increase (10%) in the seroprevalence to A/Swine/Arnsberg/1/81 was noticed, whereas the results obtained with the other strains showed little variation. Antibodies against the human H1N1 strain A/Singapore/6/86 were only found in sera collected 1987 and 1988 in rates of 1.6% and 3.0%. Serological indication of infections with the human H3N2 strains A/Victoria/1/75, A/Hong Kong/1/68 and A/Philippines/2/82 could be shown in 286 (22.6%), 178 (14.4%) and 135 (10.6%) of the serum samples. Within the three year period the rate of sera positive for antibodies against A/Philippines/2/82 increased from 6.5% to 23.0%, whereas no variation in the rates were found using the other H3N2 strains. Antibodies simultaneously against porcine (H1N1) and human (H3N2) virus strains were detected in 9.9% of all sera tested.     

商洛市平菇栽培及病虫害防治关键技术

商洛市作为我国平菇栽培的重要产区之一,拥有超过50年的平菇人工栽培历史,商洛地区平菇产业不仅在农业经济方面取得显著成就,而且深受市场和消费者喜爱。本文系统总结了近年来商洛市平菇的栽培技术及病虫害防治策略。详细介绍了商洛市平菇目前主推的栽培的季节及品种选择、栽培配方、制袋、养菌管理、出菇管理、采收等内容,分析了平菇的常见病虫害,并提出了相应的防治措施。这项研究为商洛地区平菇产业的发展壮大提供了技术支持。     

希拉穆仁草原不同放牧强度对植被群落特征和土壤团聚体的影响

[目的] 为探究草原不同放牧强度下植被群落的改变,解析群落特征变化对土壤团聚体的影响。[方法] 以希拉穆仁荒漠草原控制放牧试验区为研究对象,采用野外调查、室内分析相结合的方法,分析轻度放牧(LG)、中度放牧(MG)、重度放牧(HG)和禁牧(CK)下植被群落特征和土壤团聚体特征及二者的关系,揭示植物群落变化对土壤团聚体的影响。[结果] (1)植物群落物种数随放牧强度的增加而增加,LG的群落Shannon-Wiener多样性指数(H'')和Simpson优势度指数(D)显著低于其他放牧强度(p＜0.05)。地上生物量随放牧强度的增加而降低,HG的地上生物量显著低于其他放牧强度(p＜0.05)。不同放牧强度下地下生物量差异显著,且不同土层的地下生物量均以LG最高。(2)平均重量直径(MWD)和几何平均直径(GMD)的变化趋势与大团聚体含量一致,在0—5, 5—10 cm土层表现为随放牧强度的增加先增加后降低再增加(LG最高、MG最低)。(3)群落Shannon-Wiener多样性指数(H'')、Pielous均匀度指数(J'')、地上生物量、容重是影响大团聚体含量、MWD和GMD的显著性因子(p＜0.05)。[结论] 放牧强度对土壤团聚体稳定性具有负反馈调节作用,主要通过影响植被群落多样性指数(H''和J'')、地上生物量、土壤容重的变化而引起土壤团聚体稳定性的变化。该结果为希拉穆仁草原放牧强度选择及生态恢复工作提供理论支撑和科学依据。     

校企合作实践教育基地人才培养模式探索——以农业水利工程专业校企合作实践教育基地为例

安徽农业大学农业水利工程专业与安徽省水利科学研究院共建校企合作实践教育基地,通过建立协调育人机制,深化教育教学改革,推动教学与科研紧密结合、学校与社会密切合作,促进大学生在科学研究中学习、在社会实践中学习,提高大学生解决实际问题的实践能力和创新创业能力。通过加强内涵建设、成果共享与示范引领,在促进新工科、新农科建设,培养德智体美劳全面发展的高质量人才方面取得了一系列实践成果。     

糖苷水解酶家族基因AghyGH1a参与莲草直胸跳甲对皂苷的代谢

为了解植物化合防御物质皂苷对专食性天敌莲草直胸跳甲Agasicles hygrophila的防御作用,测定莲草直胸跳甲2龄幼虫取食皂苷后的排泄和肠道变化,利用转录组测序筛选参与皂苷代谢的候选基因,并采用 RNA 干扰技术鉴定候选基因功能。结果发现,取食皂苷导致莲草直胸跳甲幼虫排泄量增加,肠道畸变,排泄量与取食皂苷浓度正相关。转录组测序发现,与对照相比,取食 100 mmol/L皂苷处理的莲草直胸跳甲幼虫肠道有496条差异表达基因,其中261个上调基因和235个下调基因。GO分类和KEGG分类分析发现糖苷水解酶家族1（glycoside hydrolase family1,GH1）基因集中在碳水化合物水解通路。利用RNA干扰技术鉴定发现,莲草直胸跳甲AghyGH1a基因沉默可导致幼虫取食皂苷后排泄量增加,肠道内容物颜色更暗沉。表明莲草直胸跳甲AghyGH1a参与皂苷代谢。