Simple prediction of the survival of follicles in cryopreserved human ovarian tissue

This study examines the possible predictive value of the LIVE/DEAD fluorescence viability assay for evaluation of survival of cryopreserved human ovarian tissue. Ovarian tissue from ten patients was examined by LIVE/DEAD viability staining before and after cryopreservation and after freezing in a -20 C freezer (negative control). After cryopreservation with a slow freezing protocol and cryoprotectant the LIVE/DEAD assay showed 86% viable follicles (an intact oocyte and at least more than 50% of the granulosa cells alive), whereas after freezing at -20 C the survival rate was 67%. The healthy follicular loss after cryopreservation was 4%, whereas with freezing at -20 C, it was 25%. Although this assay overestimates the survival rate of cryopreserved primordial follicles, if the LIVE/DEAD assay yields greater than approximately 85% viable follicles, it can be assumed that the follicles in the cryopreserved tissue have maintained their developmental potential and that the tissue is suitable for retransplantation.     

Variation among stallions in sperm quality after single layer centrifugation

Although single layer centrifugation (SLC) selects robust spermatozoa from stallion semen, the effect of individual variation has not been studied in detail. The objective of this study was to determine the variation among stallions in the effects of SLC on sperm quality during cooled storage for up to 48 hr. Semen samples from seven stallions (18 ejaculates) were split, with one portion being used for SLC and the other serving as a control (CON). Sperm quality (kinematics, reactive oxygen species (ROS) production, membrane integrity (MI) and chromatin integrity) were analysed at 0, 24 and 48 hr using computer-assisted sperm analysis and flow cytometry. Sperm quality was better in SLC than in CON at all timepoints, especially chromatin integrity and MI (p < .0001 for both), and some categories of ROS production (e.g. proportion of live hydrogen peroxide negative spermatozoa, p < .0001), but the degree of improvement varied among stallions and type of ROS (p < .05–p < .0001). Total and progressive motility were also better in SLC samples than in CON at 24 and 48 hr (p < .0001), although the effect on sperm kinematics varied. The interaction of treatment, time and stallion was not significant. In conclusion, sperm quality was better in SLC samples than in CON, although there was considerable individual variation among stallions. The improvement in sperm quality, particularly in chromatin integrity, was clearly beneficial, and therefore the use of this technique would be warranted for all stallion semen samples.     

An experimental simulation of moisture distribution and its uniformity within the soil profile under laboratory conditions

This research is a study on the relationship of irrigation water treatments and soil moisture distribution uniformity (DU). Soil moisture distribution was based on long-term data sets that were collected using a novel electromagnetic sensor-based platform moving inside sub-surface horizontal access-tubes. The research was conducted under controlled laboratory conditions in a soil tank. The irrigation treatments regarding two case studies under dry and wet soil conditions (from permanent wilting point to field capacity) were conducted for a period of 115 and 110 days respectively. In dry soil conditions, the irrigation water treatments strongly affect the DU of soil moisture that can be achieved constantly using non-uniform irrigation treatments. In contrast, the DU of soil moisture in wet soil conditions was maintained at a high percentage and was slightly affected by irrigation treatments. However, obtaining accurate soil moisture information at a large scale over a long period can be used to improve water use efficiency.     

Nutrients in litterfall,forest floor and mineral soils in two adjacent forest ecosystems in Greece

The fluxes of masses and the nutrients Ca,Mg,K,N,P and S were determined in the litterfall of two adjacent forest ecosystems of Hungarian oak(Quercus frainetto L.)and European beech(Fagus sylvatica L.)in a mountainous area of northeastern Greece in 2010–2015.The foliar litterfall for both species reached about 70%of the total litterfall,and was significantly higher from the other two fractions(woody and rest litterfall).The fluxes of masses and nutrients were compared between ecosystems for each fraction separately.Only one significant statistical difference was found,that of K in the woody litterfall.In addition,the stocks of masses and nutrients were calculated in the forest floors and mineral soils of the two ecosystems.Likewise,the stocks of nutrients in the forest floors and mineral soils were compared between ecosystems.In the L horizon of the forest floors,statistical differences,as a result of species effect,were found for the stocks of Ca and N.In the FH horizons,the masses and all the nutrient stocks differed significantly,as the beech plot had much higher quantities of organic matter and nutrients.These higher quantities were probably due to low soil temperatures(microclimate)and high acidity in the beech plot(species effect)that slowed down decomposition.In the mineral soils,the propagation of random error derived from random errors of the individual soil layers was an important factor in the statistical comparisons.Because of the soil acidity in the beech plot,the stocks of exchangeable base cations were significantly higher in the oak plot,whereas the other nutrient stocks did not differ.     

Regional parasite density in the skin of dogs with symptomatic canine leishmaniosis

In canine leishmaniosis, the parasitic density of the skin may be important for the infection of sandflies, and increased accumulation of inflammatory cells infected with Leishmania is believed to occur in dermal areas subjected to mechanical trauma. Parasite density and inflammatory responses in the upper and lower dermis of three body sites: flank (control site), dorsal muzzle (sandfly feeding site), and footpads (mechanical stress sites) were thus investigated in 15 dogs with symptomatic leishmaniosis. Parasite density did not differ between the control and tested sites or between the upper and lower dermis, apart from the footpads where it was higher in the upper dermis, and there was no correlation with severity of the macroscopic lesions or inflammatory infiltrate, except for the lower footpad dermis. No selective accumulation of the parasite in the muzzle that would favour its transmission to sandflies occurred, and the mechanical stress imposed on the footpads was not associated with increased parasitic density, or with inflammatory infiltrate.     

State-of-the-art review of goat TSE in the European Union, with special emphasis on PRNP genetics and epidemiology

Scrapie is a fatal, neurodegenerative disease of sheep and goats. It is also the earliest known member in the family of diseases classified as transmissible spongiform encephalopathies (TSE) or prion diseases, which includes Creutzfeldt-Jakob disease in humans, bovine spongiform encephalopathy (BSE), and chronic wasting disease in cervids. The recent revelation of naturally occurring BSE in a goat has brought the issue of TSE in goats to the attention of the public. In contrast to scrapie, BSE presents a proven risk to humans. The risk of goat BSE, however, is difficult to evaluate, as our knowledge of TSE in goats is limited. Natural caprine scrapie has been discovered throughout Europe, with reported cases generally being greatest in countries with the highest goat populations. As with sheep scrapie, susceptibility and incubation period duration of goat scrapie are most likely controlled by the prion protein (PrP) gene (PRNP). Like the PRNP of sheep, the caprine PRNP shows significantly greater variability than that of cattle and humans. Although PRNP variability in goats differs from that observed in sheep, the two species share several identical alleles. Moreover, while the ARR allele associated with enhancing resistance in sheep is not present in the goat PRNP, there is evidence for the existence of other PrP variants related to resistance. This review presents the current knowledge of the epidemiology of caprine scrapie within the major European goat populations, and compiles the current data on genetic variability of PRNP.     

Perfused non-pregnant swine uteri: a model for evaluating transport mechanisms to the side bearing the dominant follicle in humans

Adequate uterine contractility and periovulatory peristalsis, interpreted as "rapid sperm transport" to the side bearing the dominant follicle, may be a precondition for successful reproduction in humans. Estrogen and progesterone fluctuate characteristically during the menstrual cycle, and their source is the dominant follicle and corpus luteum. The question is, how is the direction to the left or right side of transport mechanisms influenced? An extracorporeal perfusion model of the swine uterus was used that maintained the uterus in a functional condition and that was suitable for the study of physiological questions. The effects of side-dependent estrogen, progesterone, and estrogen plus progesterone perfusion on oxytocin-induced uterine peristalsis were assessed using two intrauterine microcatheters placed in each horn of the swine uterus. Estrogen perfusion was associated with an increase in intrauterine pressure (IUP) in a dose-dependent manner only in the estrogen-perfused horn of the swine uterus. There was a significant difference between the IUP increase measured in the estrogen-perfused horn and that in the non estrogen-perfused horn of the swine uterus. Progesterone perfusion showed no effect in general. Furthermore, progesterone antagonized the estrogen effects. This study demonstrates that side-dependent estrogen perfusion resulted in side-dependent contractility in the swine uterus perfusion system used. These observations show that estrogen stimulates uterine contractility in the estrogen-perfused uterine horn and that estrogens may be the "trigger" for the transport mechanisms to the side bearing the dominant follicle during the periovulatory phase through their locally increased concentration and distribution via the utero-ovarian counter-current system in humans.     

Omega-3-Rich Oils from Marine Side Streams and Their Potential Application in Food

Rapid population growth and increasing food demand have impacts on the environment due to the generation of residues, which could be managed using sustainable solutions such as the circular economy strategy (waste generated during food processing must be kept within the food chain). Reusing discarded fish remains is part of this management strategy, since they contain high-value ingredients and bioactive compounds that can be used for the development of nutraceuticals and functional foods. Fish side streams such as the head, liver, or skin or the cephalothorax, carapace, and tail from shellfish are important sources of oils rich in omega-3. In order to resolve the disadvantages associated with conventional methods, novel extraction techniques are being optimized to improve the quality and the oxidative stability of these high-value oils. Positive effects on cardiovascular and vision health, diabetes, cancer, anti-inflammatory and neuroprotective properties, and immune system improvement are among their recognized properties. Their incorporation into different model systems could contribute to the development of functional foods, with market benefits for consumers. These products improve the nutritional needs of specific population groups in a scenario where noncommunicable diseases and pandemic crises are responsible for several deaths worldwide.     

Control of the European grapevine moth<Emphasis Type="Italic">Lobesia botrana</Emphasis> in Greece by the mating disruption technique: A three-year survey

During 1996–1998 mating disruption trials were conducted on all three generations of the grapevine mothLobesia botrana Den. et Schiff. (Lep., Tortricidae) in a 25-ha vineyard situated at Spata, Attiki, Greece. Shortly before the beginning of the first flight period ampoule-type pheromone dispensers were set up lining a 6.5-ha rectangular plot. Around the dispenser zone a protection belt was created whereBacillus thuringiensis (Bt) and selective insecticides were applied against the second and third generation larvae. The protection belt was 35 m wide and covered 3.5 ha. The rest of the vineyard was divided into the reference plot — where conventional insecticide treatments were implemented to control both carpophagous pest generations, and the untreated control plot — which measured 1 ha. Male catches within the dispenser zone were virtually zero. Nevertheless, no difference was apparent between the percentage of mated females from within and outside of the dispenser zone. Attractant was released from the dispensers throughout the season at a rather constant rate, but by the middle of the third flight period dispensers were found to be almost empty. During the 3 years of the survey pest pressure was often at exceptionally high levels. The effectiveness of the mating disruption technique (MDT) was found to vary with year and pest generation. MDT was used against the first (anthophagous) generation with rather poor results. In the following carpophagous generations results were comparable to control measures with insecticides used and sometimes slightly better. The density of the pest population in the pheromone-treated vineyard as compared with the untreated control, was reduced on average by 67% in the second and by 57% in the third generation. MDT therefore did not accomplish sufficient crop protection. http://www.phytoparasitica.org posting Dec. 19, 2003.     

Effect of alleviating products with different mode of action on physiology and yield of olive under drought

Two years old self-rooted Koroneiki olive trees (Olea europaea L.) were subjected to two irrigation regimes, i.e. the fully irrigated and the severely water stressed trees, while they were treated with three alleviating products of different mode of action. The products used were the osmolyte glycine betaine, the antioxidant Ambiol and the heat and irradiance reflecting kaolin clay particles. The effects of product application and water regime on leaf characteristics, shoot and root growth, photosynthesis, leaf compatible solids (carbohydrates) concentration and yield were evaluated. All products applied, exhibited significant alleviating action, based on the relative alleviation index. Irrigated trees exhibited greater growth than drought stressed ones, while the ameliorating products maintained the water content of the leaves under drought conditions and resulted in lower leaf tissue density. On the other hand carbon assimilation rate, stomatal conductance and intrinsic water use efficiency were significantly reduced under drought stress, while the opposite stood for intercellular CO₂. Drought stress resulted in elevated sucrose leaf concentration, while the application of Ambiol increased stachyose concentration and that of glycine betaine did the same with the mannitol concentration. Among the alleviating products tested in this experiment Ambiol and glycine betaine had a significant positive effect on leaf water content, photosynthesis and yield under both drought and well irrigated conditions.