Therapeutic Intervention with Dietary Chitosan Nanoparticles Alleviates Fish Pathological and Molecular Systemic Inflammatory Responses against Infections

Marine bio-sourced chitosan nanoparticles (CSNP) are antimicrobial and immunomodulatory agents beneficial for fish medicine. Herein, dietary CSNP was investigated for the amelioration of the systemic inflammatory responses of an induced fish model. One hundred and forty-four rainbow trout were assigned to one pathogen-free and non-supplemented group (negative control), and three challenged groups: non-supplemented (positive control), CSNP-preventive, and CSNP-therapeutic. After a feeding experiment extended for 21 days, the organosomatic indices (OSI) and molecular aspects were assessed. After a challenge experiment extended for further 28 days, CSNP-therapeutic intervention was assessed on fish survival and systemic inflammatory responses on pathology, histo-morphology, and molecular aspects. With CSNP administration, OSI nonsignificantly decreased and the relative expression of targeted inflammatory-mediator genes was significantly increased. The CSNP-therapeutic fish showed an RPS of 80% as compared to the positive control group, and CSNP-therapeutic administration retained the highest gene expression augmentation up to 28 days after the challenge. Notably, the splenic reticulin fibers framework of the CSNP-therapeutic group retained the highest integrity among the groups during the infection. After recovery, reticulin fibers density in the CSNP-therapeutic samples was significantly higher than in the negative control group, which indicates high innate immunity. Thus, CSNP showed promising biotherapeutic features enhancing fish resistance against infections.     

Beneficial impacts of bee pollen in animal production,reproduction and health

Bee pollen (BP) is one of the most useful therapeutic products favoured by natural medicine scientists because of its possible nutritional and medical applications. It exhibits many impacts such as antimicrobial, immunostimulating, antioxidants and hepatoprotective. Furthermore, BP has some useful therapeutic features in numerous pathological situations such as its impact to normalize wound healing. Based on previous literatures, the level of BP supplement in livestock and poultry ranged from 0.1 to 20 g/kg diet. This variation depends on the species, physiological status, age and purpose of addition; so far generally, it accepted to use the level with no side effects. It has been observed that BP enhanced growth performance, immunity responses and blood variables and had hypoglycaemic activity by reducing the lipid in the blood and carcass. Also, BP contains more nutrients, which stimulate faster differentiation and proliferation of the cells of immune system of birds. Therefore, the present review recommends that BP supplementation (up to 20 g/kg diet) had possible beneficial impacts, antioxidants and protective activities on most of the production, productive and health patterns of livestock.     

Importation, colonization and establishment of Coccophagus cowperi Gir. (Hymenoptera: Aphelinidae) on Saissetia coffeae (Walk.)(Homoptera: Coccidae) in Egypt

The hemispherical soft scale, Saissetia coffeae (Walker) (Homoptera: Coccidae), is one of the most important pests attacking olive trees in Egypt. During the period 2001–2003, a total of about 300,000 individuals of the parasitoid Coccophagus cowperi Girault (Hymenoptera: Aphelinidae), obtained from India, was released at 35 sites for the biological control of S.coffeae on olive trees in Egypt. The maximum parasitism rates reached 53 and 62%, while average parasitism rates were 17.2 and 30.8% in the Marsy Mattrouh and El-Arish locations, respectively. These results indicate establishment of this parasitoid on this important economic plant in Egypt.     

氮肥配施下不同C/N作物残渣还田对红壤温室气体排放的影响

氮肥配施能够促进还田秸秆的分解,为了解其对不同C/N秸秆还田下温室气体排放的影响,采用培养实验方法,研究了油菜饼(C/N为4)、玉米秸秆(C/N为28)、水稻秸秆(C/N为41)和小麦秸秆(C/N为71)等4种不同C/N植物残渣在不同量氮肥(无氮、低氮和高氮)配施下对红壤温室气体(CO2、CH4和N2O)排放的影响。结果显示,氮肥配施增加了不同植物残渣的CO2-C累积排放量,且仅在高C/N的小麦秸秆处理中发现存在显著性差异,在低氮和高氮下CO2-C累积排放量分别达到1 271.44、1 212.83 mg·kg-1,显著高于无氮肥配施的883.40 mg·kg-1。土壤N2O累积排放量最大的为油菜饼处理组,低氮量的配施进一步增强了N2O的产生,其累积排放量达到5 550.42μg·kg-1,显著高于无氮肥配施的4 430.44μg·kg-1,然而当氮肥施用量进一步增加时却抑制了N2O的排放(3752.84μg·kg-1)。氮肥配施并未显著影响玉米秸秆和小麦秸秆处理组的N2O累积排放量。在培养期内,每一个处理均表现为CH4的吸收现象,氮肥施用能够增加土壤对CH4的累积吸收量,但差异显著性仅在对照和油菜饼处理中发现。     

Influence of organic and inorganic passivators on Cd and Pb stabilization and microbial biomass in a contaminated paddy soil

Purpose

Soil contamination with heavy metals, such as Cd and Pb, has caused severe health and environmental risks all over the world. Possible eco-friendly solutions for Cd and Pb immobilization were required to reduce its mobility through various cost-effective amendments.

Materials and methods

A laboratory incubation study was conducted to assess the efficiency of biochar (BC), zeolite (ZE), and rock phosphate (RP) as passivators for the stabilization of Cd and Pb in paddy soil as well as soil microbial biomass. Various extraction techniques were carried out: a sequential extraction procedure, the European Community Bureau of Reference (BCR), toxicity characteristic leaching procedure (TCLP) test, and single extraction with CaCl₂. The impact of passivators on soil pH, dissolved organic carbon (DOC), and microbial biomass (carbon, nitrogen, and phosphorus) was examined in the metal contaminated soil.

Results and discussion

The results showed that the exchangeable portion of Cd in soil was significantly reduced by 34.8, 21.6, and 18.8% with ZE, RP, and BC at a 3% application rate, respectively. A similar tendency of reduction in Pb soluble portion was observed by ZE (9.6%), RP (20%), and BC (21.4%) at a 3% application rate. Moreover, the TCLP leachate of Cd and Pb was apparently reduced by 17 and 30.3% with BC at a 3% application dose, respectively, when compared to the control. Soil pH, nutrients, and microbial biomass C, N, and P were significantly increased with the addition of BC, RP, and ZE passivators.

Conclusions

The results showed that the incorporation of BC, ZE, and RP significantly reduced the Cd and Pb mobility in paddy soil as well as enhanced soil nutrients and microbial biomass. Overall, among all the amendments, rice straw derived-BC performed better for Cd and Pb immobilization in paddy soil.

     

中亚热带丘陵区茶园和林地土壤春季N2O排放及其影响因素

中亚热带地区春季降雨频繁,茶园施肥量大,该季节茶园土壤氧化亚氮(N_2O)排放量较高,研究春季茶园土壤N_2O排放及其影响因子有一定意义。以中亚热带丘陵区土壤为对象,采用静态箱-气相色谱法,研究了两种植茶年限茶园和林地土壤春季N_2O排放特征及其影响因子。结果表明:茶园N_2O排放量明显高于林地,50年茶园N_2O排放量明显高于20年茶园,林地N_2O的排放量最少;50年茶园、20年茶园和林地土壤春季N_2O累积排放量分别为2.07、1.39、0.22 kg·hm-2。两种植茶年限茶园土壤N_2O排放通量均与土壤NO_3~--N含量呈显著正相关(P0.05),林地土壤N_2O排放通量则与土壤NH_4~+-N含量呈极显著正相关关系(P0.01);茶园和林地土壤N_2O排放通量均与5 d累积降雨量之间存在显著的相关性。多元逐步回归分析显示,茶园土壤N_2O排放通量受土壤温度和NO_3~--N含量影响,共同解释其48%~49%的变化;林地土壤N_2O排放通量受土壤温度和NH_4~+-N含量影响,共同解释其55%的变化。这项研究显示施肥对春季茶园N_2O排放的促进作用与降雨有关。     

In Planta - Complementation of Clavibacter Michiganensis Subsp. sepedonicus Strains Deficient in Cellulase Production or HR Induction Restores Virulence

Clavibacter michiganensis subsp. sepedonicus, a Gram positive bacterium that causes bacterial ring rot of potato, was studied in eggplant, an alternate host, using strains that differed in phenotype. Two factors affecting virulence, the ability to induce a hypersensitive response (HR) and cellulase production, were studied. A plasmid-free isolate of C. michiganensis subsp. sepedonicus that causes HR on tobacco but is unable to produce cellulase multiplied efficiently in planta, but caused only weak symptoms. In contrast, a strain that is unable to induce HR on tobacco but produces cellulase was impaired in the ability to multiply in the host and caused no symptoms. When the two non-virulent strains were coinoculated into eggplants, typical disease symptoms developed. This enhancement was not due to formation of a new phenotype or significant increases in population density of either of the strains. Our results suggest that both cellulase production and the ability to induce HR are required for a successful infection process and disease induction by C. michiganensis subsp. sepedonicus. Our results additionally suggest that the ability to induce HR on non-host plants is required for multiplication in the host plant, whereas cellulase expression is necessary for induction of disease symptoms.     

Nutritional significance of amino acids,vitamins and minerals as nutraceuticals in poultry production and health – a comprehensive review

Nutraceuticals have gained immense importance in poultry science recently considering the nutritional and beneficial health effects of their constituents. Besides providing nutritional requirements to birds, nutraceuticals have beneficial pharmacological effects, for example, they help in establishing normal physiological health status, prevent diseases and thereby improve production performance. Nutraceuticals include amino acids, vitamins, minerals, enzymes, etc. which are important for preventing oxidative stress, regulating the immune response and maintaining normal physiological, biochemical and homeostatic mechanisms. Nutraceuticals help in supplying nutrients in balanced amounts for supporting the optimal growth performance in modern poultry flocks, and as a dietary supplement can reduce the use of antibiotics. The application of antibiotic growth enhancers in poultry leads to the propagation of antibiotic-resistant microbes and drug residues; therefore, they have been restricted in many countries. Thus, there is a demand for natural feed additives that lead to the same growth enhancement without affecting the health. Nutraceuticals substances have an essential role in the development of the animals’ normal physiological functions and in protecting them against infectious diseases. In this review, the uses of amino acids, vitamins and minerals as well as their mode of action in growth promotion and elevation of immune system are discussed.     

CO2 and N2O emissions in response to dolomite application are moisture dependent in an acidic paddy soil

Journal of Soils and Sediments - Liming is the most widely used agricultural practice for acidic paddy soils amelioration, which is usually applied before rice transplanting. Both liming and soil...