棘孢木霉葡聚糖酶基因Glu1的克隆及原核表达

从棘孢木霉(Trichoderma asperellum)ACCC30536中克隆获得一个葡聚糖酶基因Glu1,其cDNA全长984bp,编码327个氨基酸。该葡聚糖酶属于Glyco-hydro-12家族,推测为β-1,4-葡聚糖酶,与深绿木霉IMI 206040的Glycohydro-12家族蛋白(XP_013940397.1)有91%相似性,且亲缘关系较近。运用qRT-PCR技术检测9种诱导条件下棘孢木霉Glu1基因的表达水平,结果表明,Glu1基因能参与棘孢木霉对山新杨(Populus davidiana×P.alba var.pyramidlis)或杨树病原菌的识别。构建原核表达载体并获得重组蛋白rGlu1。酶活特性分析结果表明,该酶最适pH为4.5,最适温度为45℃,且酶活性随诱导时间延长逐渐升高,在5h达到稳定。     

四种菊酯类杀虫剂对枸杞蚜虫线粒体膜流动性的影响

为明确菊酯类杀虫剂对昆虫线粒体膜流动性的影响,以1,6-二苯基-1,3,5-己三烯（1,6-diphenyl-1,3,5-hexatriene,DPH）为荧光探剂,采用荧光偏振法研究了4种菊酯类杀虫剂对枸杞蚜虫Aphis sp.线粒体膜流动性的影响。结果表明,4种菊酯类杀虫剂对枸杞蚜虫线粒体膜流动性均有显著影响,其荧光偏振度显著增大,线粒体膜流动性显著减小。当药剂浓度为100 μmol/L时,对照组枸杞蚜虫线粒体膜的荧光偏振度为0.239,高效氯氰菊酯的影响最大,线粒体膜的荧光偏振度为0.315,联苯菊酯的影响最小,线粒体膜的荧光偏振度为0.287。4种菊酯类杀虫剂对线粒体膜流动性的影响都存在一定的浓度效应,当药剂浓度在0~100 μmol/L范围内,浓度越大影响越大。4种菊酯类杀虫剂在不同温度下对枸杞蚜虫线粒体膜流动性的影响不同,高效氯氰菊酯、联苯菊酯和甲氰菊酯对线粒体膜流动性的影响基本不受温度影响,而氯菊酯在高温条件下产生的影响大,在低温条件下产生的影响小,表现为明显的正温度系数。表明DPH可以作为荧光探剂研究枸杞蚜虫线粒体膜的流动性。     

优质高产不结球白菜新品种“抗寒1号”的选育

"抗寒1号"是以自交不亲和系14LY106为母本,14LY108为父本配置而成的一代杂种。株型较直立,早春播种生长40d时,株高22cm,开展度22cm,叶色绿,叶面平展,叶柄绿,叶柄宽3.2cm,厚0.68cm,单株质量68g,品质脆嫩,味浓,纤维少。每667m~2产量2 400kg以上。该品种抗病毒病、抗霜霉病,抗寒、耐抽薹,适合北方地区早春和秋冬季栽培。     

生物素测定方法的应用现状

<正>生物素,又称维生素H,是一种水溶性B族维生素。生物素的测定方法很多,早在20世纪40年代,国外就报道了利用微生物法测定生物素的含量。随着科学技术的发展,国内外报道了许多新的测定方法,主要有同位素稀释法、     

Effect of urea supplementation on performance and safety in diets of Dorper crossbred sheep

This study was conducted to evaluate the efficacy and safety of dietary urea in sheep rations having a 50:50 concentrate:roughage ratio. Sixty‐four Dorper × thin‐tailed Han crossbred ram lambs with an average body weight of 30.8 (±0.02) kg were randomly divided into four groups of 16 sheep each, and each group was fed one of the following diets: a basal diet (CON), or CON supplemented with 0.5% (0.5UTM), 1.5% (1.5UTM) or 2.5% (2.5UTM) urea. Growth performance, carcass characteristics, non‐carcass offals, meat quality and peptic tissue lesions were assayed. The average daily weight gains for CON, 0.5UTM, 1.5UTM and 2.5UTM were 216, 218, 200 and 170 g, respectively, with the CON and 0.5UTM groups higher than 2.5UTM group (p < 0.05). Sheep from the 2.5UTM treatment had a significantly lower dry matter intake (1.29 kg/day) than those from the CON and 0.5UTM treatments (1.42 and 1.43 kg/day, p < 0.05), and the feed conversion ratio in the 2.5UTM group was the highest (p < 0.05). Carcass characteristics, including shrunk body weight, empty body weight, hot carcass weight, dress percentage, and the absolute or relative weight (% body weight) of heart, liver, spleen, lung and kidney, were not altered by the treatments (p > 0.05). The muscular pH of 2.5UTM was higher than that of CON (5.68 vs. 5.52, p < 0.05), and shear force in 0.5UTM was lower compared with CON and 2.5UTM (p < 0.05). The anatomical structure lesions in kidneys became more serious with the increasing dietary urea concentrations, with the 2.5UTM animals showing the most severe lesions compared with CON animals. Therefore, supplementary urea as a non‐protein nitrogen source for sheep should not exceed 1.5% of ration having a 50:50 concentrate:roughage ratio to ensure efficacy and safety.     

Mutant-prevention concentrations of enrofloxacin for Escherichia coli isolates from chickens

OBJECTIVE: To investigate the development of enrofloxacin resistance among Escherichia coli isolates obtained from chickens by determining mutant-prevention concentrations (MPCs) and sequence the quinolone resistance-determining regions (QRDRs) of gyrA and parC genes in selected isolates. SAMPLE POPULATION: 15 chicken-derived E coli isolates. PROCEDURES: For all isolates, MPC and minimal inhibition concentration (MIC) of enrofloxacin were determined. The MPCs and maximum serum drug concentrations attained with enrofloxacin doses recommended for treatment of E coli infections in chickens were compared. Mutation frequencies and QRDR sequence changes in gyrA and parC were also determined. RESULTS: In 2 of 15 E coli strains, MPCs were low (0.016 and 0.062 microg/mL), MPC:MIC ratios were 2 and 4, and the GyrA and ParC proteins had no mutations. In 9 susceptible isolates with a GyrA point mutation, MPCs ranged from 2 to 16 microg/mL. For isolates with double mutations in GyrA and a single mutation in ParC, MPCs were > 32 microg/mL (several fold greater than the maximal plasma concentration of enrofloxacin in chickens); mutation frequencies were also much lower, compared with frequencies for single-mutation isolates. CONCLUSIONS AND CLINICAL RELEVANCE: For E coli infections of chickens, MPC appears to be useful for determining enrofloxacin-dosing strategies. The high MPC:MIC ratio may result in enrofloxacin-treatment failure in chickens infected with some wild-type gyrA E coli isolates despite the isolates' enrofloxacin susceptibility (MICs 0.125 to 1 microg/mL). For infections involving isolates with high MPCs, especially those containing mutations in gyrA and parC genes, treatment with combinations of antimicrobials should be adopted.     

基于KPCA-WLSSVM的建筑能耗预测模型

为降低建筑能耗影响因素间复杂相关性对模型性能的影响,建立了一种基于KPCA-WLSSVM的建筑能耗预测模型。利用核主元分析(KPCA)对输入变量进行数据压缩,消除变量之间的相关性,简化模型结构;进一步采用加权最小二乘支持向量机(WLSSVM)方法建立建筑能耗预测模型,同时结合一种新型混沌粒子群-模拟退火混合优化(CPSO-SA)算法对模型参数进行优化,以提高模型的预测性能及泛化能力。通过将KPCA-WLSSVM模型方法应用于某办公建筑能耗的预测中,并与WLSSVM、LSSVM及RBFNN模型相比,实验结果表明,KPCA-WLSSVM模型方法能有效提高建筑能耗预测精度。     

土壤水分对稻米外观品质的影响

水稻的分蘖期、长穗期、抽穗后1～10d、11～20d、21～30d及1～30d,用负压式土壤湿度计监测,分别进行土壤水势为-30～-35kPa、-60～-65kPa的控水处理,收获后对优势粒、中位粒、劣势粒进行粒形和垩白测定,发现在不同土壤水分下S品种长穗期和抽穗后1～10d控水显著抑制了籽粒伸长,也不同程度的抑制了籽粒增宽和长厚,以S4处理受抑制的程度最为严重;Y品种主要是长穗期控水显著抑制了粒长、粒宽和粒厚;两品种的多数处理不同程度的提高了粒厚,这在优势粒中更明显;土壤水分对粒长/宽的影响很小。S品种各处理垩白率、垩白度多低于CK,Y品种各处理垩白率、垩白度多高于CK。对两个品种来说长穗期是影响外观品质的主要水分敏感期,节水栽培应从品种选择入手。     

胞外多聚物在污泥颗粒化过程中的作用研究

本文研究了胞外多聚物(ECP)在污泥颗粒化过程中的作用。试验采用小试UASB反应器,以人工葡萄糖配水作为进料。测定结果表明,在颗粒污泥培养过程中,随着颗粒污泥的形成、生长和成熟,污泥ECP含量逐渐下降,从开始的6.36减小到30mg/gVSS。当反应器酸化、污泥性能变差或运行不稳定时,ECP含量则表现为急剧上升,达70mg/gVSS。这些结果说明污泥颗粒化过程中ECP所起的作用不大。对有关ECP的影响及作用机理,以及污泥颗粒化机制,文章进行了分析讨论。     

正反转组合式水稻宽苗带灭茬播种机设计与试验

针对稻麦两茬轮作区,小麦收获时间短、水稻插秧费时费力、直播整地要求高的问题,利用反转灭茬技术和主动防拥堵技术,同时借鉴小麦宽幅精播技术,设计了一种正反转组合式水稻宽苗带灭茬播种机,一次完成旋耕、灭茬、防堵、深施肥、宽苗带播种、覆土和镇压功能。在稻麦两熟区进行了试验,结果表明,反转旋耕装置能很好的灭茬,正转清草装置能有效防堵,在正反旋耕的配合作用下,种床土壤细碎,播种覆土均匀,播种深度和施肥深度变异系数分别为4.58%和2.40%,种肥垂直间距变异系数为4.72%;平均苗带宽度为138.4 mm,与理论设计宽度差异不显著;不同苗带宽度上种子分布有差异但不显著,符合设计要求;苗带宽度对水稻生长有影响,苗带两侧有效分蘖和成穗率显著高于苗带中间(P0.05);机具的通过性满足农艺要求。