不同杨树品系的营养成分与青杨天牛发生的关系(英文)

在吉林省松原市建立了杨树对青杨天牛抗性的栽植实验区,调查了10个不同品系杨树的青杨天牛发生量,并对各杨树品系枝条的主要营养成分进行了分析。结果显示:各杨树品系间青杨天牛的发生量以及枝条中氨基酸含量之间的差异均极其显著;杨树品系青杨天牛的发生量与可溶性总糖及含水量之间有显著正相关关系(相关系数分别为0.737和0.684),但与全氮及蛋白态氮含量之间无显著相关关系(相关系数分别为-0.166和-0.100)。     

杨树品系对杨干象抗性研究初探

杨树不同品系对杨干象抗性差异很大,以美洲黑杨为亲本的多数外来品种对杨干象抗性差,也使杨干象种群迅速扩大。该文根据调查的3年生和4年生连续2 a的不同杨树品系的平均虫口密度,根据聚类分析的方法将其划分为高感虫品系、感虫品系、低抗虫品系、中抗虫品系和高抗虫品系5个级别,结合杨树品系的类型、形态特征等进行分析,为生产上选用杨树抗性品系和减少杨干象大发生的危害及抗虫育种等方面提供参考。     

Transgenic poplar with enhanced growth by introduction of the ugt and acb genes

The transgenic poplar (Populus tremula L.) was obtained by transfer of the ugt and acb genes via triparental mating, which was employed to deliver large fragments of TDNA as a cluster. Freshly harvested seeds of local poplar were placed on MS agar medium and plantlets were obtained. After 1 year of subcultivation, plantlets were infected with a transconjugant of triparental mating with target ugt and acb genes into axillary buds. The transformed sprouts so obtained were cut and subcultivated on agar medium with an addition of 0.6 mg/l indole-3-butyric acid as an auxin source. The transformed sprouts showed GUS activity and resistance to gentamycin and kanamycin. The integrity of the target ugt and acb genes into poplar genome was demonstrated via PCR and Southern blot hybridisation. The transgenic poplar plants revealed a higher growth energy, corresponding to a higher content of IAA as opposed to control plants. Both transgenic and non-transformed plants were potted into soil for outdoor acclimatisation and subsequently transferred to earth in beds. Growing outside during 3 years, the transgenic poplar demonstrated a higher growth rate with fast bud and branch development.     

农林业转基因抗虫性育种的研究、应用情况概述

概述了转Bt杀虫蛋白基因棉花和杨树的抗虫性研究、应用及其作用特点，明确了对它们的利用原则。     

Changes through time in traits of poplar clones in selection trials

Growth, survival, stem characteristics, resistance to wind, and canker disease of 51 poplar clones were evaluated at age 9 and 10 years, and compared to measurements at age 3 in two trials on the Lower Valley of the Colorado River, Argentina. About 30% of the clones in both trials had large changes in growth rankings at age 9 and 10 compared to rankings at age 3. Canker incidence increased about 4-fold between age 3, and measurements at age 9 and 10. Stem form was in general satisfactory, and wind incidence was almost nil. In trial 1, clones 588–1, Conti 12, 582–41, and 582–5 occupied the upper positions in the growth rankings at age 3 and 10, and the clone 588–1 ranked first both in growth and canker incidence in 1998. Stem growth trajectories of clones with similar growth rates did not differ among clones with, and without canker disease. In trial 2, the range in growth among clones was narrower than in trial 1, and canker disease, although of minor incidence, was present in most clones at age 9. Some of the P. deltoides clones in the trials had similar growth, and less canker disease than the Euroamerican clones commonly grown in the region, but progressive canker incidence should be addressed with additional selection trials.     

Relationship between stomatal behavior and characteristics of photosynthesis and transpiration ofAdenophora lobophylla andA. potaninii at different altitudes

The photosynthesis and transpiration characteristics ofAdenophora lobophylla andA. potaninii, as well as stomatal behavior such as stomatal size, stomatal density, stomatal open and stomatal conductivity were measured at different altitudes. The relationship between the photosynthesis and transpiration characteristics and the stomatal behavior was analysed by correlation coefficient and path coefficient analysis with altitude changes. The results showed that the influences of stomatal behavior were not evident on the photosynthesis and transpiration characteristics ofA. Lobophylla, but evident on that ofA. potaninii. This study is supported by the National Natural Science Foundation of China (No. 39391500). Responsible editor: Zhu Hong     

青杨天牛生物学及发生规律的研究

本文通过对青杨天牛发育进度的观测和对青杨天牛其它生物学特性的研究，摸清了青杨天牛成虫羽化、卵孵化的规律及青杨天牛对不同杨树品种危害程度的差异，为青杨天牛的监测与防治提供了科学依据。     

Relationship between resistance to vole browsing and content of ether extract in the bark of larch species and hybrids

We determined the survival rate of tree families produced by the intra- and interspecific crossing of larch species damaged by browsing of vole (Clethrionomys rufocanus bedfordiae Thomas) and the contents of ether extracts (EE) in their bark. The average survival ofLarix gmelini var.japonica Pilg. (L. gmelini)×L. gmelini (G×G) was highest, followed by the hybridL. gmelini×L. leptolepis Gordon (G×L). The families of the hybrid G×L, which is considered suitable for silviculture in Hokkaido, Japan, showed significant differences in survival rates, suggesting that it is possible to produce improved varieties of the hybrid which are more resistant to vole browsing. The EE contents of the bark were under strong hereditary control, and the rank of the EE content among the various families hardly fluctuated between the location of the test fields. The correlation coefficient between the survival rates and the EE contents was highly significant (r=0.89,n=24,p<0.01), indicating that the EE content in the bark may be used as a possible index of resistance or susceptibility to vole browsing in larch plantations. A part of this report was presented at the 106th Annual Meeting of the Japanese Forestry Society, at Sapporo, April, 1995.     

Steady-state nutrition of hybrid poplar grown from un-rooted cuttings

Fertilizing plantations of fast-growing tree crops, in manners which supply nutrients at rates that match plant demand and maintain stable internal plant nutrient ratios, can maximize biomass production and carbon sequestration while reducing fertilizer wastage and pollution. Our objectives were to determine nutrient ratios of common hybrid poplar (Populus trichocarpa Torr. and Gray × Populus deltoides Marsh.) (T × D) clones under steady-state nutrition, and to determine if incremental additions of fertilizer were more successful than conventional (evenly-split) fertilizer additions in maximizing biomass production and inducing stable nutrient ratios. Un-rooted cuttings of three T × D clones (49–177, DTAC-7, 15–29) were grown under a conventional regime and a modified-exponential fertilization regime at three application rates (1.8, 3.7 or 7.4 g N plant⁻¹). Above- and below-ground biomass and nutrient concentrations were measured after one growing season. There were few differences in total plant biomass between conventional and modified-exponential fertilization regimes, but for one clone, biomass accumulation equivalent to the highest rate under the conventional regime was achieved with the medium rate of the modified-exponential regime. Stable nutrient ratios (at conditions consistent with steady-state nutrition) were: 100N:14P:50K (49–177), 100N:13P:49K (DTAC-7) and 100N:12P:60K (15–29).     

不同品系欧洲报春的抗寒性研究

在不同低温处理下,分别对引进的3个品系欧洲报春叶片和花进行相对电导率及游离脯氨酸含量测定,分析其抗寒性的差异,结果表明:3个品系的欧洲报春花叶片伤害度、半致死温度和游离脯氨酸含量变化较为相符,其叶片和花的耐寒性强弱为:丹若娃的叶片>里拉的叶片>妃纯的叶片;妃纯的花>丹若娃的花>里拉的花,与其在露地栽培中观察到的结果相符。     

不同杨树品系光合作用的研究

对13个杨树品系1 a生苗木的净光合速率、蒸腾速率进行测试与分析,结果表明:不同品系的净光合速率差异较大,杂1号、杂2号、A5-13、A5-17的净光合速率较对照大青杨分别高出15.07%、17.15%、5.25%和11.52%。长势较好的6个品系和对照的净光合速率日变化明显,杂1号、杂2号、A5-17、自选1号和对照呈现"双峰"曲线,峰值出现在10:00时和14:00时;A5-13、辉南5号呈现"单峰"曲线,峰值出现在14:00时。6个品系蒸腾速率的日变化基本相同,在11:00~13:00时达到最大值,之后迅速下降;同对照比较,A5-13、A5-17、杂1号、杂2号为高光合、高蒸腾类型,辉南5号为高光合、低蒸腾类型,自选1号为低光合、匀速蒸腾类型。在光合有效辐射为0~800μmol.m-2.s-1范围内,6个品系的净光合速率随着光合有效辐射的增加而增强,基本呈正相关;在光合有效辐射为1 500μmol.m-2.s-1左右时,达到光饱和点。     

Insect inhabitants of old larval galleries of<Emphasis Type="Italic"> Saperda populnea</Emphasis> (L.) (Coleoptera: Cerambycidae) in Bulgaria

The complex of insect inhabitants of old larval galleries of Saperda populnea (L.) (Coleoptera: Cerambycidae) was studied during the period 2000–2003 in 18 localities in Bulgaria. As a result, 32 insect species were reared from old S. populnea galls: Metopoplax origani (Kolenati) (Heteroptera: Lygaeidae), Malachius sp. (Coleoptera: Malachiidae), Agrilus pratensis pratensis (Ratzeburg) (Coleoptera: Buprestidae), Xylocleptes bispinus (Duftschmidt) (Coleoptera: Scolytidae), Crossocerus cetratus (Shuckard), Crossocerus megacephalus (Rossi), Crossocerus nigritus (Lepeletier & Brullé), Crossocerus acanthophorus (Kohl), Passaloecus brevilabris Wolf, Passaloecus gracilis (Curtis), Psenulus schencki (Tournier), Solierella compedita compedita (Piccioli), Trypoxylon figulus figulus (Linnaeus), Trypoxylon fronticorne fronticorne Gussakovskij (Hymenoptera: Crabronidae), Cleptes schmidti Linsenmaiser, Trichrysis cyanea (Linnaeus) (Hymenoptera: Chrysididae), Clistopyga incitator (Fabricius), Ctenochira sanguinatoria (Ratzeburg), Liotryphon crassisetus (Thomson), Nemeritis fallax (Gravenhorst), Mesochorus georgievi Schwenke, Campoletis sp. (Hymenoptera: Ichneumonidae), Charmon extensor (Linnaeus) (Hymenoptera: Braconidae), Eupelmus urozonus Dalman (Hymenoptera: Eupelmidae), Perilampus aeneus (Rossius) (Hymenoptera: Perilampidae), Trichiocampus grandis (Lepeletier) (Hymenoptera: Tenthredinidae), Janus luteipes (Lepeletier) (Hymenoptera: Cephidae), Cydia corollana (Hübner) (Lepidoptera: Tortricidae), Fiebrigella brevibucca (Duda) (Diptera: Chloropidae), Eustolomyia hilaris (Fallén) (Diptera: Anthomyiidae), Senotaina sp. (Diptera: Sarcophagidae) and Heringia vitripennis (Meigen) (Diptera: Syrphidae). Amongst them, 27 species were established as new inhabitants of old S. populnea galls. Five species (C. schmidti, N. fallax, M. georgievi, P. aeneus and J. luteipes) were recorded for the first time in Bulgaria. The dwellers of old S. populnea galls belong to the following ecological groups: insects using galls as a place to develop (3 species); insects nidificating in empty galls (10 species); insects using old galls as a pupating place (1 species); insects using empty galls as overwintering shelters (3 species); predators and parasites of primary dwellers of empty galls (13 species); or insects with uncertain ecological status (2 species).     

杨树不同品种插穗自身含水量与扦插成活的关系

以中黑防、晚花杨、小黑杨14号3个杨树品种为试验材料,设计自然失水24 h、48 h、72 h三个梯度(处理)研究插穗自身含水量与扦插成活的关系.结果表明:在相同的自然失水时间里,晚花杨失水率最大,自然失水处理72 h,失水率可达30.7%,小黑杨14号为29.6%,中黑防也达到了27.9%.中黑防在穗条自身失水率达到30%时,生根与成活率才会显著下降;而小黑杨14号和晚花杨在穗条自身失水率达到20%时,生根与成活率就会显著下降.     

Calling behaviour and male response towards sex pheromone of poplar moth <Emphasis Type="Italic">Condylorrhiza vestigialis</Emphasis> (Lepidoptera: Crambidae)

The calling behaviour of virgin females Condylorrhiza vestigialis Guenée, 1854 (Lepidoptera: Crambidae) and the female pheromone gland extract activity were studied under laboratory conditions. Most of the females started calling from their first scotophase period after emergence. Maximum calling occurred between the seventh and tenth hours of the scotophase period. The length of the calling increased with age until the fourth scotophase, but the onset of calling time did not differ with age. The number of calling bouts increased significantly with age, but the mean duration of each calling bout (20.8 min) did not vary with age. Extracts of pheromone glands evaluated in a Y-tube olfactometer attracted significantly more males than control, 70 and 30%, respectively. Gas chromatographic–electroantennogram detection (GC–EAD) analysis of these extracts indicated the presence of a single EAD-active peak, the putative sex pheromone of the species. This pheromone compound may be suitable for monitoring populations densities of C. vestigialis, and for detection of the onset of the seasonal flight period. An efficient pheromone is of importance also with respect to current attempts to develop new control methods for this important pest of Populus spp. in Brazil.     

THEORY AND APPLICATION OF CUTTAGE SEEDLING AND AFFORESTATION ON POPLAR

Itisoneofproblemsthatwoodsqualityandquantityperareaareraisedthroughstudyingstanddensity.Accordingtoprincipleofformingarationalstandstructure,wetrytoachieveatheoreticalmodelofonetreephotosynthesisnutritionarea(PNA)orTPNAconnectedwithpopulusphotosynthesisorganform(PoForPPOF)andtogetrationalmanagementdensityusingmathematicalways.Assci-enceandtechniquedevelopon,therearemanyexamp1esandmethodstostudyafforestationbymathematicaltoo1s.Butsofar,manyshortagesthatthefieldworkisgreatandthecostishighan…     

不同复合经营模式对杨树蛀干害虫天牛控制效果的调查研究

杨树人工林复合经营模式对杨树蛀干害虫天牛控制效果和复合经营的效益结果表明,5种复合经营模式与纯林相比,杨树天牛虫口密度下降了87．94％-97．16％,有虫株率下降了81．11％-96．17％,复合经济收益有显著增加。同时,综合评估了不同复合经营模式的效益。分析显示,以林渔模式的综合效益最佳;林农（油）模式的控制天牛效果最好,林牧模式次之。采用林菌模式可获得更长期的复合经营效益。     

Proteomic analysis of the G-layer in poplar tension wood

Angiosperm trees bend their stems by forming tension wood at the upper side of leaning stems. Most tension wood has a cellulose-rich G-layer in the innermost surface of the fiber cell wall. Strong tensile stress is considered to occur in the G-layer. This study undertook to identify the proteins involved in G-layer formation and function through a proteomic analysis of G-layer-localized protein. G-layers of poplar were loosened by sonication and isolated as doughnut-shaped pieces of thinly sliced transverse sections. The proteins, once extracted with urea/detergent solution, were separated by two-dimensional polyacrylamide gel electrophoresis, and 110 spots were subjected to liquid chromatography tandem mass spectrometry (LC/MS/MS). A database search for these spots’ mass spectrum patterns identified 72 proteins. In addition, all peptide digestion mixtures of G-layer proteins were separated by strong cation exchange chromatography and 39 proteins were identified using LC/MS/MS analysis. Proteins involved in wall formation, such as lignin biosynthesis-related protein, xyloglucan endotransglucosylase, and fasciclin-like arabinogalactan protein, were notably detected in the G-layer.     

青杨楔天牛在拉萨发生危害情况的初步调查

青杨楔天牛在拉萨1a发生1代。以幼虫在被害肿瘤内越冬。翌年3月底-4月上旬化蛹，4月下旬-5月成虫羽化，5月中下旬产卵。西藏自治区目前仅发现该虫在曲水县聂当乡自治区试验林场危害北京杨、新疆杨。     

Antisense expression of a caffeic acidO-methyltransferase ofStylosanthes humilis in transgenic poplar: Effect of expression onO-methyltransferase activity and lignin composition

Poplar (Populus tremula) was transformed with a construct carrying an antisense caffeic acidO-methyltransferase (COMT) cDNA (pOMT8) from a tropical pasture legume,Stylosanthes humilis. pOMT8 shows 83% overall homology to the corresponding COMT gene (pPCLA) of poplar. Of the 200 putatively-transformed plants regenerated on selective media after co-cultivation of poplar stem explants withAgrobacterium tumefaciens harbouring a CaMV 35S-antisensepOMT8 construct, a subset of 20 plants were randomly chosen for further analysis. PCR and Southern blot analysis demonstrated the stable integration of T-DNA into the genome of these plants. Antisense expression ofpOMT8 resulted in reductions in total COMT activity in the majority of the transgenic plants with the lowest total COMT activities (61–70% of untransformed control plants) being observed in four transgenic plants. The composition of lignin in transgenic plants was also changed, as detected by reductions in the content of syringyl units using infrared spectroscopy. However, no changes were found in the amount of insoluble lignin in transgenic plants as compared to untransformed control plants. These results indicate the potential of thepOMT8 gene to partially suppress COMT activity and modify the composition of lignin in transgenic poplar. This work was partly supported by General Management of Turkish Pulp and Paper Mills.     

Variation analysis of growth traits of four poplar clones under different water and fertilizer management

To determine appropriate quantities of water and fertilizer required for early growth of hybrid poplar cuttings,we recorded the growth traits of four clones grown under four factors(irrigation and nitrogen,phosphorus,and potassium fertilizers),each with four levels,using an orthogonal experimental design.A logistic model was used to estimate growth in height.The growth curves for tree height were sigmoid,and the model R^2 values were greater than 0.9,which indicated that the fit was highly significant.ANOVA results for tree height and basal diameter indicated that all sources of variance showed significant differences(p<0.001).The average tree height and basal diameter for all the four clones under the different treatments ranged from 155.39 to 235.04 cm,and from 13.71 to 17.42 mm,respectively.A highly positive correlation between the extreme k value and tree height was observed,suggesting that the k value was an accurate estimation of tree height.For model parameters,the earliest average time point for the onset of the rapid growth period of poplar clones was 131 d,and the highest average increment in tree height during the rapid growth period was 138.78 cm.The highest average tree height for all clones under each factor was 219,210.51,200,and 201 cm when treated with either 1200 mL of water applied every third day,3 g of nitrogen,0 g of phosphorus,or 0 g of potassium,respectively.The most suitable treatment for the early growth of hybrid poplar cuttings,as suggested by the developed logistic model,was 1200 mL of water applied every third day and three applications of 1 g nitrogen(in the form of CH4N2O).