Silage and milk production: a comparison between concentrates containing different amounts of protected protein as supplements for silage of high digestibility

Grass silage made in late May from S24 perennial ryegrass was offered ad libitum to eight Ayshire cows in a 16-week feeding experiment. The silage had a DM concentration of 244 g kg⁻¹, contained 163 g crude protein (kg DM)⁻¹ with a ruminal degradability of 0.77 and had an in vitro DOMD concentration of 678 g kg⁻¹. In addition, four concentrates each containing 167 g soya-bean meal kg⁻¹ were consumed at a mean daily rate of 6.43 kg DM per cow. The soya-bean meal was either untreated, or 'protected' by formalin and mixed in the following proportions, 100:0; 66:34; 34:66; and 0:100 respectively, in the four concentrates. The daily intakes of silage DM were not significantly different on the four treatments and averaged 90 kg DM per cow, giving a mean total daily DM intake of 32.4 g kg⁻¹ live weight. The milk yields were not significantly different on the four treatments and averaged 23.9 kg ⁻¹ The treatments had small and non-significant effects on milk composition and live weight. It is concluded that with a high-digestibility, well-preserved grass silage of satisfactory protein content the inclusion of 'protected' protein in the supplementary concentrate had no beneficial effects on milk production.     

Silage and milk production: a comparison between supplements of barley and soybean meal offered with white clover silage

Silage made from Blanca white clover was offered ad libitum to four Friesian cows in a 12-week changeover experiment. The silage contained 990 g white clover DM (kg DM)⁻¹ with 254 g DM kg⁻¹ and 243 g CP (kg DM)⁻¹ The pH was 3.98 and the in vitro DOMD concentration 680 g kg⁻¹. The silage was the sole feed in the control treatment; in the other three treatments it was supplemented with barley, soybean meal, and a mixture of these feeds supplying 8.1, 2.7 and 7.8 kg DM per cow respectively. The daily intakes of silage DM were 19.3, 13.5, 17.5 and 13.5 kg per cow, and the daily milk yields were 26.3, 28.0, 28.6 and 27.6 kg per cow on the control, barley, soybean and barley plus soybean treatments respectively. It is concluded that the white clover silage had an excellent fermentation and a large potential for milk production.     

Effects of raw soy flour feeding in weanling pigs: comparison with rats and monkeys

Feeding of raw and heated soy flour (RSF & HSF) for 4 or 8 weeks to weanling swine was compared with feeding of casein diets. There was essentially no growth in pigs fed RSF, whereas body weight increased 4.5 fold over 8 weeks with either casein or HSF. No change in pancrease weight as percent body weight was found in any group in either experiment. Both total pancreatic RNA and DNA were decreased in RSF groups, although RNA and DNA/mg pancreas were increased by the RSF diets. Total pancreatic protein, as well as protein/mg, were decreased in RSF-fed pigs compared to casein and HSF. Pancreatic trypsin, chymotrypsin, lipase, and amylase, and fecal trypsin were decreased in RSF-fed pigs to varying degrees compared to either HSF or casein. HSF and casein performance was equivalent in all parameters measured. Results of these experiments are compared with results of feeding similar diets to rats and monkeys.     

Digestive enzymes of the crustaceans Munida and their application in cheese manufacturing: a review

Crustaceans Munida (fam. Galatheideae, ord. Decapodi) were fished in the Southern Adriatic Sea and their proteolytic activities were characterized and tested for potential application in cheese manufacturing. Enzymes extracted from whole crustaceans, mainly serine proteases, showed high caseinolytic and moderate clotting activities. Analysis by 2D zymography of the digestive enzymes extracted from Munida hepatopancreas, showed the presence of several isotrypsin- and isochymotrypsin-like enzymes in the range of 20-34 kDa and 4.1-5.8 pI. Moreover, specific enzymatic assays showed the presence of aminopeptidases and carboxypeptidases A and B. Overall, optimum activity was achieved at pH 7.5 and 40-45 °C. Caseinolytic activity, determined both spectrophotometrically and by SDS gel electrophoresis, indicated higher activity on β-casein than on α-casein. Miniature cheddar-type cheeses and Pecorino-type cheeses were manufactured by adding starter, rennet and Munida extracts to milk. Reverse-phase HPLC and MALDI-ToF mass spectrometry showed a more complex pattern of proteolytic products in cheeses made using Munida instead of chymosin. Munida extracts were found to degrade the chymosin-derived β-casein fragment f193-209, one of the peptides associated with bitterness in cheese. In conclusion, Munida digestive enzymes represent a promising tool for development of new cheese products and shorten cheese ripening when used either alone or in addition to calf rennet.     

Implications of climate change for grassland in Europe: impacts, adaptations and mitigation options: a review

Climate change associated with greenhouse gas (GHG) emissions may have important implications for Europe's grasslands. Projected scenarios indicate that increased temperatures and CO₂ concentrations have the potential to increase herbage growth and to favour legumes more than grasses, but changes in seasonal precipitation would reduce these benefits particularly in areas with low summer rainfall. Further implications for grasslands may arise from increased frequency of droughts, storms and other extreme events. Potential farm-scale adaptive responses to climate change are identified. Grassland agriculture also contributes to GHG emissions, particularly methane and nitrous oxide, and management of grassland affects net carbon balances and carbon sequestration. Management options are identified for mitigating grassland's contribution to GHG emissions which need to be developed in a holistic way that also considers other pressures.     

Progeny testing for resistance to potato virus Y: a comparison of susceptible potato cultivars for use in testcrosses with resistant parents

Summary For potato breeding or genetical research purposes, the number of copies of a dominant major gene for resistance to potato virus Y in a parent clone can be determined by test-crossing with a susceptible parent and observing the segregation ratio of resistant to susceptible seedlings in the progeny. In a comparison of different susceptible cultivars for use in test-crosses for this purpose, their progenies differed in the clarity of symptoms and parental phenotype proved an unreliable guide. Cvs Maris Piper, Arran Peak and Dr MacIntosh were found to be suitable tester parents, Pentland Squire less so and cv. Désirée was confirmed as having a major gene conferring incomplete resistance. It was found advisable to test-cross with more than one susceptible parent.     

Water and oil-repellent coatings of perfluoro-polyacrylate resins on cotton fibers: UV curing in comparison with thermal polymerization

UV curing of perfluoro-alkyl-polyacrylate resins able to impart water as well as oil-repellency to cotton fabrics was studied in comparison with conventional thermal polymerization. The process was assessed through weight gain and gel content measurements while the properties conferred to cotton fabrics were determined in terms of water and oil contact angles, moisture adsorption, and water vapor permeability. The polymerization yields were of the same order (>80 %) of those obtained with thermal curing as well as the high contact angles with water (>127°) and oil (>118°) even at low resin add-on (3 %). UV cured resins yielded oil contact angles mostly higher than 120° denoting super oil-repellent surfaces. Moreover the water and oil-repellency was adequately maintained after washing. The moisture adsorption of finished fabrics was lower than that of untreated cotton, but slightly higher for UV cured than thermally treated fabrics. Water vapor transmission rate showed that the finish treatment, thermal as well as by UV curing, does not reduce the breathability of the original cotton. DSC analysis demonstrated that the fiber pyrolysis is affected by the polymer add-on, while FTIR-ATR spectra of all finished fabrics showed typical peaks of ester and C-F groups. XPS analysis showed small differences between thermal and UV curing coatings with each resin, while coatings with the lowest percentage of fluorine groups did not affect the water and oil-repellency.     

Temperate intercropping of cereals for forage: a review of the potential for growth and utilization with particular reference to the UK

Cereals grown for forage are increasing in importance in the rations of ruminants in the UK and have the potential to supply high proportions of energy-rich forage in their diets. However, such diets usually require some degree of protein supplementation. Crop mixtures, generally referred to as intercrops, have the potential to boost the forage protein content of diets as well as having a number of agronomic benefits. In this paper, the growth and utilization of annual intercrops is reviewed with particular reference, where feasible, to temperate regions. General agronomic and feeding issues associated with cereal intercrops are outlined together with the practicalities of field-scale management of intercrops in highly mechanized systems. A number of cereal-based intercrop combinations are also considered, concentrating specifically on their value for forage production. The future potential for different combinations is discussed and research recommendations made.     

Antifungal activity of flavonoids from Heteropterys byrsonimifolia and a commercial source against Aspergillus ochraceus: In silico interactions of these compounds with a protein kinase

To contribute to the development of new methods to reduce the contamination of coffee beans with Aspergillus ochraceus and ochratoxin A, a toxin produced by this fungus, the present work initially aimed to select plant extracts active against A. ochraceus. Among the extracts from 43 plant species, the most active was the one obtained from leaves of Heteropterys byrsonimifolia. This extract was subjected to a fractionation process that resulted in the purification of four flavonoids, among which the most active against the fungus was rutin, which presented a minimal inhibitory concentration (MIC, 32.5 μg/mL) close to the value obtained for the commercial fungicide benzalkonium chloride (7.84 μg/mL). In vitro studies with the isolated flavonoids and other four from commercial sources showed that the MIC for the flavonoid chrysin is equal to that observed for rutin, and results from an in silico study suggested that these flavonoids act against A. ochraceus by binding to a protein kinase produced by this fungus. Consequently, this enzyme and the studied flavonoids are potentially useful for the development of new products to control A. ochraceus in coffee beans.     

Factors influencing the response in intake of silage and animal performance after wilting of grass before ensiling: a review

This review examines the response in intake of silage and animal performance after wilting of grass before ensiling. It uses data from eighty‐five published comparisons. Initial analysis identified those variables having the greatest influence on the increase in dry‐matter (DM) intake of grass silage owing to wilting. Total water loss, drying rate of the wilted material and ammonia‐N concentration of the unwilted material were the variables most closely and positively correlated to the proportional increase in DM intake as a result of wilting, with r² values of 0·344, 0·393 and 0·240 respectively. There was little evidence of curvilinearity in any of the relationships. Stepwise regression analysis was used to identify the best multiple linear regressions for predicting the proportional increase in intake owing to wilting. Factors associated with the extent and rate of water loss in the field (total water loss and drying rate) and the quality of the unwilted material ensiled and its subsequent fermentation pattern (DM digestibility, crude protein, ammonia‐N content of unwilted silage) were the key variables in the relationship. Subsequent analysis related the increase in output of milk energy of dairy cows and liveweight gain of beef cattle to the same independent variables. This indicated that variables shown to describe the response in DM intake in the previous analysis were among those most closely related to the increases in animal performance owing to wilting of grass. To assess whether the increase in animal performance owing to wilting could be explained as a direct response to increased metabolizable energy (ME) intake of wilted compared with unwilted silages, linear relationships were derived between the increase in milk and beef production parameters and the increase in ME intake owing to wilting. From these, it was concluded that there was no clear evidence that wilting reduced the efficiency of utilization of ME in beef or dairy cattle. The present study showed that the increase in milk energy output (measured in MJ) per increase in ME intake (measured in MJ) owing to wilting was linear, with a slope of 0·19. This response from additional ME intake owing to wilting is in line with what would be anticipated when increasing ME intake by other methods.     

Grazing legumes in Europe: a review of their status, management, benefits, research needs and future prospects

In many parts of Europe there has been a net decline in the use of forage legumes since the 1980s, despite the reputed value of legumes for low‐input livestock production systems. The political environment within which livestock farming in much of Europe operates (Common Agricultural Policy) is shifting the balance of economic advantage towards legumes and away from high usage of inorganic fertilizer. This has already been found for legume and grass–legume silages when compared with grass silages with a potential economic gain for farmers averaging 137 € ha^?1, corresponding to an annual benefit for the European livestock farming sector of as much as € 1300 million. Recent literature has shown that legume‐based grazing systems have the ability to reduce environmental problems by increasing the efficiency of N use and by avoiding a high transient surplus of soil mineral N. From the perspective of livestock nutrition, when forage legumes contain moderate levels of secondary compounds, such as condensed tannins and flavonoids, they offer considerable advantages including increased efficiency of N utilization within the digestive tract, reduced incidence of bloat hazard and higher resilience to parasites. Nevertheless, these benefits are partially counterbalanced in both temperate and Mediterranean regions by difficulties in establishment, maintenance and management under grazing. To gain knowledge on mixed grass–legume pastures, further research is required on: (i) the development of sustainable systems of livestock production which can maintain sward persistence and agricultural production under environmental stress; (ii) increasing knowledge of soil–plant–animal relations for a wide range of leguminous species, and under different soil types and climatic situations; and (iii) the benefits for consumers of food produced from low‐input livestock production systems.     

The extent of semi-natural grassland communities in lowland England and Wales: a review of conservation surveys 1978–96

The extent of semi-natural grassland has diminished considerably across lowland landscapes of England and Wales during the second half of the twentieth century. Locating, describing and evaluating the dwindling cover has been a major challenge for conservationists. A concentrated vegetation survey effort at grassland sites has been mounted within different parts of Britain since the late 1970s. Plant community recognition has benefited considerably from the development of the contemporary National Vegetation Classification, and its widespread adoption permits national inventory of comparable vegetation data. Findings of a range of surveys (ninety-eight in total), undertaken between 1978 and 1996 in England and Wales covering different forms of unimproved lowland grassland, are collated and reviewed. Vegetation data were abstracted from internally published survey reports. Calcicolous and neutral grasslands have been covered more thoroughly than acidic and wet or marshy grasslands. Cover data are summarized at community level. Overall estimates from survey results indicate that there are some 27 500–40 000 ha of calcicolous grassland, 7500–15 000 ha of unimproved neutral pasture and hay meadow, 8000–15 000 ha of acidic grassland and 9000–17 500 ha of wet grassland in lowland England and Wales; these represent only 1–2% of the cover of permanent lowland grassland. Some communities have additional representation in heathlands, mires and upland environments. Although they require further refinement, the cover data for individual communities provide a context for assessing priorities in site-based and agri-environment conservation programmes. It is concluded that, as well as arresting further depletion, it will be necessary to restore and expand lowland grassland habitats to counteract the negative impacts of fragmentation and isolation of various community types, such as the Centaureo–Cynosuretum, which is widely but thinly distributed. Habitat rehabilitation schemes also need to assimilate local patterns of community diversity characteristic of both wet and dry grasslands. It is suggested that reversal of the recent successional trends that followed relaxation of grazing at certain sites might produce a more appropriate balance in the relative cover of coarse tall grasslands and fine short turf. Vegetation surveys provide a source of spatial data for identifying local aggregations of semi-natural grassland remnants.     

A review of our current understanding of parasite survival in silage and stored forages,with a focus on Fasciola hepatica metacercariae

Fasciola hepatica, the parasitic liver fluke, is a re‐emerging zoonotic infection and an important cause of morbidity and mortality in ruminant livestock worldwide. A significant animal welfare concern, fasciolosis also has a detrimental impact on food security, with the effects of sub‐clinical infection on growth rate and milk yield estimated to cost the UK cattle industry £40.4 million annually. It is understood that up to 50% of infective F. hepatica metacercariae may overwinter on pasture and remain viable to infect grazing livestock the following spring. However, the infection risk posed by feeding grass silage to livestock remains largely unknown as the majority of previous studies are outdated in both experimental design and analysis of ensiled metacercariae viability. Anecdotal reports of fasciolosis outbreaks in housed livestock have reignited interest in F. hepatica metacercariae survival during modern ensiling processes and more importantly if they retain viability within stored forages. Consequently, a comprehensive review of the available literature is required in order to identify knowledge gaps and highlight future research opportunities regarding the role of silage in the transmission of F. hepatica.     

Jasmonates and their role in plant growth and development,with special reference to the control of potato tuberization: A review

Jasmonic acid and closely related compounds show growth regulating properties in plants. They have been found in more than 30 plant species. In this paper we review what is known about the jasmonate biosynthetic pathway, biological activities, and mode of action. We also discuss the possible role of jasmonates in potato tuberization.     

Superior bio-efficacy of a combined formulation of carbendazim and mancozeb in inducing defense responses in chilli seedlings against Sclerotium rolfsii Sacc. in comparison with methyl jasmonate

The objective of this work was to investigate the efficacy of a prepackaged combined formulation, Companion (carbendazim 12% + mancozeb 63% WP), sole application of carbendazim 50% WP, manozeb 75% WP and methyl jasmonate (MeJA), an inducer of systemic acquired resistance on disease severity and their role in post-infectional defense responses in chilli seedlings against Sclerotium rolfsii. Seeds were treated for 8 h with MeJA (2.5 mM and 5.0 mM) and each of fungicides (500 ppm), and were sown in pots containing soil and fungal inocula (95:5 w/w). At 15 days after sowing maximum defense against fungal infection was exhibited by Companion comparably followed by the sole application of carbendazim and mancozeb. MeJA reduced percent mortality of S. rolfsii-infected chilli seedlings significantly as compared to the inoculated control. Assessment of peroxidase (POX) and esterase (EST) at 15 days after sowing revealed the increased activity under inoculated condition. Highest POX activity in MeJA treatments (5 mM > 2.5 mM) was followed by the Companion treatment. Highest EST activity was registered in Companion treatment. The zymogram of POX isozymes showed over-expression of POX 2 and POX 4 isoforms, and induction of POX 1 isoform in inoculated treatments. On the other hand, that of EST isozymes showed induction of EST 1 isoform in Companion, carbendazim and MeJA treatments. All EST isoforms were over-expressed in Companion-treated seedlings. Both fungicides and MeJA showed significant effects on disease severity, induction of defense enzymes and isozyme pattern in S. rolfsii-infected chilli seedlings. Contact and systemic fungicides under the experiment demonstrated differential responses. The combination formulation was superior in disease control to application of the fungicide components individually. They compared favourably with MeJA in induction of defense-related enzyme activities. All these findings are new with respect to the chilli-S. rolfsii host–pathogen interaction system, S. rolfsii representing the sclerotial basidiomycetes in particular.     

Agricultural intensification and the loss of habitat,species and amenity in British grasslands: a review of historical change and assessment of future prospects†

Grassland in Britain is a plagioclimax vegetation type. All lies on land originally cleared from forest and, without some form of management, would revert to forest through a process of natural succession. Traditionally managed, unsown grasslands nonetheless resemble more natural grasslands in other parts of the world and contain a substantial proportion of our native flora and fauna. They are also often important for recreational activities. Agricultural intensification over a long period has led to a loss in their extent, species and amenity. Reconciling agricultural and environmental objectives in grassland management is very difficult because increased fertility and production invariably leads to species loss. Current overcapacity in the agricultural industry is leading to the switching of some agricultural support from food production to countryside management. As a result some arable and intensively managed grass is being managed more extensively. However future production technologies may be so efficient that very many fewer stock are required with the result that much marginal grassland may revert once again to forest.     

Recycling of sewage sludge to grassland: a review of the legislation to control of the localization and accumulation of potential toxic metals in grazing systems

The recycling of sewage sludge or biosolids to grassland is strategically important in the European Union (EU) and its use is tightly regulated to control the risk of pathogen transfer to animals and the food chain. Sewage sludges not only contain valuable concentrations of beneficial nutrients, but also elevated concentrations of potentially toxic metals (PTM) compared with average background concentrations in the soil. The EC and UK regulations refer to six PTM, Cd, Pb, Cu, Zn, Ni and Hg, with provisional regulations for Cr, that have to be controlled to prevent detrimental effects on soil and animal health. Despite these regulations, there is still a danger that grazing animals may ingest elevated concentrations of PTM. Biosolids may adhere to herbage after the surface application of sewage sludge to grassland. The repeated surface application of sewage sludge to grassland can lead to elevated concentrations of PTM at the soil surface that may be ingested, together with soil and herbage, by grazing ruminants. This may lead to accumulation of Cd or Pb in liver or kidney. The risk to the human food chain is considered to be low, but the impact on the environment is still unknown. There is little information, for example, on the amount of soil and PTMs that may become incorporated into conserved grass. At present EU and UK legislation and voluntary codes of practice have been developed to protect animal from pathogens in sewage sludge and to minimize any potential risks from accumulation of PTM. The background and implementation of the legislation are examined in this review, and the source and mechanisms of accumulation of PTM by the grazing animal are evaluated.     

Study of the impact of wheat flour type,flour particle size and protein content in a cake-like dough: Proton mobility and rheological properties assessment

The study of food products is always a challenge due to the number of components involved and the interactions that may occur between them. Water is a particular ingredient which interacts with all hydrophilic compounds, although affinities may differ for limiting water amount. During this study, results obtained using ¹H NMR on cake dough were compared in terms of the effects of flour type (soft or medium hard), the addition of gluten (5%–20%) and the use of soft flour fractions (flour particle fractions smaller or larger than 50 μm). T₂ values and the signal intensities of different proton populations were studied as a function of the wheat protein contents of dough samples. Physicochemical characterization methods were used to better understand how the origin and particle size of flour might impact the hydration properties and mobility of a model system. Increasing the protein content in dough samples was related to an increase of the mobility of fat protons and of the least mobile proton population (relaxation times ranging from 175 to 180 ms and from 5 to 7 ms, respectively).