养分调控对大豆可溶性糖积累、分配及产量的影响

采用田间试验方法,设置1个优化施肥处理和2个习惯施肥处理,分析了大豆不同生育时期各部位可溶性糖积累和分配的特点.结果表明,优化施肥处理通过施用缓释尿素,减少前期的氮肥供应量而增加后期供应量,有效延长叶片功能期,增加光合产物的合成,增加籽粒中的可溶性糖积累量,提高可溶性糖在籽粒中的分配比例,提高产量.     

Identification of novel soybean oil content-related genes using QTL-based collinearity analysis from the collective soybean genome

Soybean is a global principal source of edible plant oil. As more soybean oil-related quantitative trait loci (QTLs) have been located in the collective genome, it is urgent to establish a classification system for these distributed QTLs. A collinear platform may be useful to characterize and identify relationships among QTLs as well as aid in novel gene discovery. In this study, the collinearity MCScanX algorithm and collective soybean genomic information were used to construct collinearity blocks, to which soybean oil-related QTLs were mapped. The results demonstrated that 666 collinearity blocks were detected in the soybean genome across 20 chromosomes, and 521 collinearity relationships existed in 231 of the 242 effective soybean oil-related QTLs. This included 214 inclusion relationships and 307 intersecting relationships. Among them, the collinearity among QTLs that are related to soybean oil content was shown on a maximum of seven chromosomes and minimum of one chromosome, with the majority of QTLs having collinearity on two chromosomes. Using overlapping hotspot regions in the soybean oil QTLs with collinearity, we mined for novel oil content-related genes. Overall, we identified 23 putatively functional genes associated with oil content in soybean and annotated them using a number of annotation databases. Our findings provide a valuable framework for elucidating evolutionary relationships between soybean oil-related QTLs and lay a foundation for functional marker-assisted breeding relating to soybean oil content.     

Genome-wide association and linkage mapping strategies reveal genetic loci and candidate genes of phosphorus utilization in soybean

Insufficient available phosphorus in soil has become an important limiting factor for the improvement of yield and quality in soybean. The mining of QTLs and candidate genes controlling soybean phosphorus utilization related traits is a necessary strategy to solve this problem. In this study, 11 phosphorus utilization related traits of a natural population of 281 typical soybean germplasms and a recombinant inbred line(RIL) population of 270 lines were evaluated under different phosphorus condit...     

Identification of QTLs Associated with Resistance to Pseudomonas syringae pv.Glycinea in Soybean (Glycine max (L.) Merr)

Soybean bacterial spot disease caused by Pseudomonas syringae pv.Glycinea which is a bacterial disease seriously affects soybean yield.Ten soybean germplasms and recombinant inbred lines (RILs) population were used to identify the resistant trait after inoculated with P.sg (P.sgneau001) in this study.High-density genetic mapping was obtained by specific length amplified fragment sequencing (SLAF-seq) of 149 RILs population which was derived from the crossing between Charleston and Dongnong594.The results indicated that 10 germplasm resources had four resistant germplasms included highly resistant cultivar Charleston,four susceptible varieties included Dongnong594 and two moderately resistant cultivars.Five quantitative trait locus (QTLs) were detected in RILs population by the composite interval mapping (CIM) method,and located on Linkage Group (LG) D1b (chromosome two),LG C2 (chromosome six) and LG H (chromosome 12),respectively.LOD scores ranged from 2.68 to 4.95 and the phenotypic variation percentage was from 6% to 11%.Six candidate genes were detected,according to the result of gene annotation information.Four of them had relationship with protein kinase activity,protein phosphorylation and leucine rich repeat (LRR) transmembrane protein,which had high expression after inoculated with P.sg by qRT-PCR.     

大豆PP2C家族基因鉴定与响应盐胁迫的转录组分析

植物蛋白磷酸酶2C(PP2C)能够通过影响植物体内多种生物学过程在环境胁迫响应中发挥重要作用.为研究栽培大豆PP2C家族成员在盐胁迫中的功能,通过生物信息学手段结合转录组学分析对栽培大豆PP2C家族进行了系统探究.在栽培大豆中共鉴定出126个PP2C家族成员,并将其在进化树上分为11个亚家族,同一亚家族的成员具有类似的...     

Identification, inheritance and QTL mapping of root traits related to tolerance to rhizo-spheric stresses in soybean (G. max (L.) Merr.)

A sample of soybean accessions (Glycine max (L.) Merr.) from Huanghe-Huaihe-Haihe and Middle-Lower Changjiang Valleys in China was used to identify their tolerance to rhizo-spheric stresses, including drought, aluminum toxin and low phosphorus. A total of 15 accessions highly tolerant to at least one of the abiotic stresses were screened out. The correlation between drought tolerance and the relative values of total root length, root volume and dry root weight (relative to dry plant weight) were all significant at 0.01 level, respectively. So did for the correlation between aluminum toxin tolerance and the stress to non-stress ratios of the number of lateral roots, tap root length, total root length, root volume and dry root weight. The inheritance study on the above three root traits related to drought tolerance under segregation analysis indicated that between the two parents of the recombinant inbred line (RIL) population of (Kefeng 1 × Nannong 1138-2), the relative values of dry root weight, total root length and root volume were controlled by two major genes plus polygenes with their major gene heritability values 62.26%–91.81% and polygene heritability values 2.99%–24.75%, respectively, and for the latter two traits, the two major genes linked together with recombination value 4.30% and 1.93%, respectively. The inheritance study on the five root traits related to aluminum toxin tolerance revealed that between the two parents of the recombinant inbred line (RIL) population of (Bogao × NG94-156), the stress to non-stress ratios of lateral root number, tap root length, total root length and dry root weight were controlled by three major genes plus polygenes with their major gene heritability values 80.22%–91.81% and polygene heritability values 3.52%–11.39%, while the stress to non-stress ratio of root volume was controlled by three major genes with their major gene heritability value 93.44%. The (Kefeng 1 × Nannong 1138-2) RIL population was also used for mapping QTLs of relative dry root weight, total root length and root volume related to drought tolerance. Five, three and five QTLs located on Linkage group N6-C2, N8-D1b+W, N11-E and N18-K for each of the three traits, respectively, were identified. Each of the traits appeared to have one locus (Dw1, Rl1, Rv1) with relatively large effect in comparison with their other loci, and the three loci in above parentheses were located in the same region STAS8_3T-STAS8_6T on N6-C2 with a same distance to the flanking markers. Thus, Dw1, Rl1, and Rv1 even might be a same locus and performed as pleiotropic of a same gene. The results between segregation analysis and QTL mapping appeared relatively consistent, therefore could be used for verification each other. Part of the results was published in Chinese in Acta Genetica Sinica, 2005, 32(8): 855–863 [部分译自: 遗传学报] Note: Junyi Gai and Shouyi Chen are joint senior authors with equal responsibility.     

RTM-GWAS方法应用于大豆RIL群体百粒重QTL检测的功效

【目的】为全面解析大豆重组自交系群体中调控百粒重性状的QTL体系,将限制性两阶段多位点全基因组关联分析方法(RTM-GWAS)和不同定位方法进行比较、优选,为后续候选基因体系探索及分子标记辅助育种设计提供依据。【方法】利用以科丰1号和南农1138-2为亲本衍生的重组自交系群体NJRIKY的427个家系,通过由全基因组39 353个SNP构建的3 683个SNPLDB标记及3个环境下的百粒重表型数据,选用复合区间作图法(CIM)、基于混合线性模型的全基因组关联分析方法(MLM-GWAS)和RTM-GWAS 3种方法检测百粒重QTL,通过QTL数目和总的表型变异解释率比较检测功效,挑选最佳定位结果进行NJRIKY群体中的百粒重遗传体系解析。通过候选基因体系的功能注释,挖掘调控大豆百粒重的生物学途径。【结果】科丰1号与南农1138-2的百粒重差异较大,多环境平均数分别为9.0和17.9 g,遗传变异系数为12.4%,遗传率为85.4%,适用于百粒重性状的遗传解析。比较3种方法定位结果表明RTM-GWAS方法表现最佳,检测QTL数目最多(57个),解释表型变异最多(70.78%)。而CIM仅检...