Total Phenolic and Phytosterol Compounds and the Radical Scavenging Activity of Germinated Australian Sweet Lupin Flour

In addition to their favourable nutritional profile, legumes also contain a range of bioactive compounds such as phenolic compounds and phytosterols which may protect against chronic diseases including cancer and cardiovascular disease. Germination of some legume seeds has been previously reported to increase the concentration of the bioactive compounds. In this study, the effect of germination of Australian Sweet Lupin (ASL) seeds for 9 days on the concentration of some bioactive compounds and the radical scavenging activity in the resulting flour was determined. The concentration of total phenolic compounds in methanolic extracts of germinated ASL flour was determined using Folin Ciocalteu reagent and phytosterols in oil extracts were analyzed by gas–liquid chromatography. The methanolic and oil extracts were also used to determine radical scavenging activity toward 2,2-diphenyl-1-picrylhydrazyl. In the methanolic extracts of germinated ASL flour, phenolic contents and the antioxidant activity were significantly increased following germination (700 and 1400 %, respectively). Analysis of the oil extracts of germinated ASL flour revealed that the concentration of phytosterols and the antioxidant activity were also increased significantly compared to ungerminated ASL flour (300 and 800 %, respectively). The relative proportion of phytosterols in germinated ASL flour was: β-sitosterol (60 %), stigmasterol (30 %) and campesterol (10 %). Germination increases the concentration of bioactive compounds and the radical scavenging activity in the germinated ASL flour.     

甜玉米籽粒总RNA提取方法的比较

[目的]探索获得高质量、高纯度的甜玉米籽粒总RNA的提取方法.[方法]以授粉后20 d的超甜玉米系SY01籽粒为试验材料,比较了RNAiso法、Trizol法、CTAB法3种方法对甜玉米籽粒总RNA的提取效果,用琼脂糖凝聚电泳、Nanodrop2000、Agilent 2100 Bioanalyzer检测了提取的RNA...     

珍汕97B改良新不育系与珍汕97A的制种产量比较研究

以6个珍汕97B改良新不育系珍品A、珍农567A、农711A、珍农256A、珍农257A、珍农060A为母本,珍汕97A为对照,以48-2为父本,进行了杂交制种产量比较试验.结果表明,与对照(珍汕97A/48-2)相比,不育系珍农257A、珍农256A、珍品A和珍农567A分别与48-2配制F1的制种产量极显著增加,平均产量分别为3.13,3.07,3.06和2.97t/hm2,分别比对照增产14.31％,12.36％,11.81％和8.45％;这4个不育系柱头外露率和异交结实率均高于珍汕97A是制种产量显著提高的主要原因.