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1.
The aim of this work was to compare the kinetic parameters of acid phosphatase (EC 3.1.3.2.) extracted from two forest soils under oak or pine. Soil was extracted with 4 mM CaCl2 and the extract was divided into two fractions by filtration: one >0.2 μm containing microbial cells and soil particulates, and the other <0.2 μm containing fine particles and dissolved organic compounds of soil. The >0.2 μm fraction had higher Km (0.26–0.82 vs. 0.12–0.39) and Vmax (0.07–0.79 vs. 0.06–0.16) values than the <0.2 μm fraction, indicating a higher enzyme-substrate affinity and smaller amount of enzyme in fine particles and dissolved organic matter.  相似文献   

2.
Abstract

The Langmuir parameters of orthophosphate (OP) and pyrophosphate (PP) sorption for the ammoniated tropical soils were determined. Positive linear relationships between OP and PP sorption maxima and amounts of anhydrous NH3 added were noticed. Indexes of bonding energy of OP and PP increased exponentially as ammoniation level of the soils increased from 33 to 100% of ammonia retention capacity.  相似文献   

3.
In order to study effects of sodium bicarbonate (NaHCO3) stress in irrigation water on photosynthetic characteristics and iron (Fe) and sodium (Na+) translocation content of pomegranate plants, a factorial experiment was conducted based on completely randomized design with three cultivars of pomegranate (“Gorch-e-Dadashi,” “Zagh-e-Yazdi,” and “Ghermez-e-Aliaghai”) and four concentrations of NaHCO3 (0, 5, 10, and 15 mM), with three replications. The results of plant analysis indicated that NaHCO3 affected chlorophyll index, Fv/Fm, and performance index (PI) in upper and lower leaves of shoots and also the translocation of Na+ and Fe. The results also showed that Fe translocation from root to shoot reduced at 15 mM level of NaHCO3. The highest Na translocation and the lowest Fe translocation were observed in Zagh-e-Yazdi and Ghermez-e-Aliaghai cultivars, respectively. The ratio of sodium/potassium (Na+/K+) in stems was higher than that in roots and leaves, and the observed chlorophyll content of upper leaves was also lower than that of lower leaves. Based on the measured parameters Gorch-e-Dadashi cultivar showed less relative sensitivity than other cultivars to NaHCO3 of irrigation water through maintaining the lower Na+ transport to the shoots, and improvement of Fe transport to shoots.  相似文献   

4.
To study the feasibility of using magnetic techniques for monitoring soil pollution in Shanghai, magnetic properties and heavy metals in the topsoils in an urban site (Songnan Town) and a less-urbanized agricultural site (Luojing Town) in Baoshan District, Shanghai, were studied. Compared with the background, magnetic signals of the urban topsoils are extremely enhanced with magnetic susceptibility (χlf) from 127.3–1959 × 10− 8 m3 kg− 1; while those of the agricultural topsoils are only slightly increased. However, both the urban and agricultural topsoils contain few pedogenic SP grains, as indicated by their low χfd% (< 3.6%). Ratios of χarm/SIRM, χarm/χlf and SIRM/χlf indicate that the grain size of magnetic minerals in the urban topsoils is significantly coarser than that in the background and the agricultural topsoils. Furthermore, the urban topsoils show low coercivity and magnetic soft behaviors, as indicated by higher SOFT%, lower HARD%, higher IRM300 mT/SIRM (close to 1) and lower IRM− 200 mT/SIRM (close to − 1). It suggests that the urban topsoils have received some coarse ferrimagnetic particles. Heavy metals are highly enriched in the magnetic fractions of the topsoils. Geochemical properties of the magnetic fraction of the urban topsoils are significantly different from those of the agricultural topsoils, further indicating that the extra magnetic minerals accumulated in the urban topsoils are neither inherited from soil parent materials nor from pedogenic processes, but originate from anthropogenic activities. The significant correlations between heavy metals and χlf, χarm, SIRM, SOFT and HIRM of the topsoils in the district indicate that the magnetic techniques can be used for monitoring soil pollution in Shanghai. The soils with χlf from 39–50 × 10− 8 m3 kg− 1 in the district are tentatively defined as “slightly polluted soils”; those with χlf > 50 × 10− 8 m3 kg− 1 are defined as “polluted soils”.  相似文献   

5.
In studying the basal respiration, microbial biomass (substrate-induced respiration, SIR), and metabolic quotient (qCO2) in western red cedar (Thuja plicata Donn ex D. Don)-western hemlock [(Tsuga heterophylla Raf.) Sarg.] ecosystems (old-growth forests, 3- and 10-year-old plantations) on northern Vancouver Island, British Columbia, Canada, we predicted that (1) soil basal respiration would be reduced by harvesting and burning, reflecting the reduction in microbial biomass and activities; (2) the microbial biomass would be reduced by harvesting and slash-burning, due to the excessive heat of the burning or due to reduced substrate availability; (3) microbial biomass in the plantations would tend to recover to the preharvesting levels with growth of the trees and increased substrate availability; and (4) microbial biomass measured by the SIR method would compare well with that measured by the fumigation-extraction (FE) method. Decaying litter layer (F), woody F (Fw) and humus layer (H) materials were sampled four times in the summer of 1992. The results obtained supported the four predictions. Microbial biomass was reduced in the harvested and slash-burned plots. Both SIR and FE methods provided equally good estimates of microbial biomass in the samples [SIR microbial C (mg g-1)=0.227+0.458 FE microbial C (mg g-1), r=0.63, P=0.0001] and proved suitable for microbial biomass measurements in this strongly acidic soil. Basal respiration was significantly greater in the old-growth forests than in the young plantations (P<0.05) in both F and H layers, but not in the Fw layer. For the 3- and 10-year-old plantations, there was no difference in basal respiration in F, Fw, and H layers. Basal respiration was related to changes in air temperature, precipitation, and the soil moisture contant at the time of sampling. The qCO2 values were higher in the old-growth stands than in the plantations. Clear-cutting followed by prescribed burning did not increase soil microbial respiration, but CO2 released from slash-burning and that contributed from other sources may be of concern to increasing atmospheric CO2 concentrations.  相似文献   

6.
A total of 400 surface soil(0–15 cm) samples were collected from cultivated soils representing four soil series,namely,Hariharapur,Debatoli,Rajpora and Neeleswaram in Orissa,Jharkhand,Himachal Pradesh and Kerala states of India,respectively,and were analyzed to measure the contents of total and extractable Mn and Fe,to establish the relationship among total and extractable Mn and Fe and soil properties,and to characterize the spatial distribution pattern of Mn and Fe in some cultivated acid soils of India. The contents of total as well as extractable Mn and Fe varied widely with extractants and soil series. However,the amounts of Mn or Fe extracted by diethylene triamine penta-acetic acid(DTPA),Mehlich 1,Mehlich 3,0.1 mol L-1 HCl and ammonium bicarbonate DTPA(ABDTPA) were significantly correlated with each other(P 0.01). Based on the DTPA-extractable contents and the critical limits(2 mg Mn kg-1soil and 4.5 mg Fe kg-1 soil) published in the literature,Mn and Fe deficiencies were observed in 7%–23% and 1%–3% of the soil samples,respectively. The content of soil organic carbon(SOC) had greater influence on total and DTPA-extractable Fe than did soil pH. Geostatistical analysis revealed that total and DTPA-extractable Mn and Fe contents in the acid soils were influenced by soil pH,SOC content,and exchangeable cations like potassium,calcium and magnesium. Spatial distribution maps of total and DTPA-extractable Mn and Fe in soil indicated different distribution patterns.  相似文献   

7.
Abstract

Simple steam distillation methods are described for determination of ammonium N and nitrate N in acid KMnO4 solution used to absorb NH3, NO and NO2 evolved from soils. They involve use of MgO for distillation of ammonia and of FeSO4, Ag2SO4, and MgO for reduction of nitrate to ammonia. The methods are rapid and precise, and they permit nitrogen‐15 analysis of NH3‐N and (NO + NO2)‐N evolved from soils.  相似文献   

8.
In an incubation experiment with flooded rice soil fertilized with different N amounts and sampled at different rice stages, the methane (CH4) and carbon dioxide (CO2) production in relation to soil labile carbon (C) pools under two temperature (35°C and 45°C) and moisture (aerobic and submerged) regimes were investigated. The field treatments imposed in the wet season included unfertilized control and 40, 80 and 120 kg ha?1 N fertilization. The production of CH4 was significantly higher (27%) under submerged compared to aerobic conditions, whereas CO2 production was significantly increased under aerobic by 21% compared to submerged conditions. The average labile C pools were significantly increased by 21% at the highest dose of N (120 kg ha?1) compared to control and was found highest at rice panicle initiation stage. But the grain yield had significantly responded only up to 80 kg ha?1 N, although soil labile C as well as gaseous C emission was noticed to be highest at 120 kg ha?1 N. Hence, 80 kg N ha?1 is a better option in the wet season at low land tropical flooded rice in eastern India for sustaining grain yield and minimizing potential emission of CO2 and CH4.  相似文献   

9.
The thermodynamic parameters of the enzymes catalase, dehydrogenase, casein-protease, α-N-benzoyl-l-argininamide (BAA)-protease, urease, Carboxymethyl (CM)-cellulase, invertase, β-glucosidase and arylsulphatase, were investigated in grassland soils from a European temperate-humid zone (Galicia, NW Spain). The effect of temperature on enzyme activity was determined at 5, 18, 27, 37, 57 and 70 °C. The temperature-dependence of the rate of substrate hydrolysis varied depending on the enzyme and soil. In general, the soil containing the least amount of organic matter (OM) showed the lowest enzyme activity for all temperatures and enzymes, whereas soils with similar OM contents showed similar levels of activity for the entire temperature range. Temperature had a noteworthy effect on the activity of oxidoreductases. Product formation in the reaction catalyzed by dehydrogenase increased with increasing temperature until 70 °C, which was attributed to chemical reduction of iodonitrotetrazolium violet (INT) at high temperatures. Catalase activity was not affected above 37 °C, which may be explained either by non-enzymatic decomposition of hydrogen peroxide or by the fact that catalase has reached kinetic perfection, and is therefore not saturated with substrate.The Arrhenius equation was used to determine the activation energy (Ea) and the temperature coefficient (Q10) for all enzymes. The values of Ea and Q10 for each enzyme differed among soils, although in general the differences were small, especially for those enzymes that act on substrates of low molecular weight. In terms of the values of Ea and Q10 and the differences established among soils, the results obtained for those enzymes that act on substrates of high molecular weight differed most from those corresponding to the other enzymes. Thus the lowest Ea and Q10 values corresponded to BAA-protease, and the highest values to CM-cellulase and casein-protease. Except for catalase in one of the soils, the values of Ea and Q10 for the oxidoreductases were similar to those of most of the hydrolases. In general, the effect of temperature appeared to be more dependent on the type of enzyme than on the characteristics of the soil.  相似文献   

10.
福寿螺(Pomacea canaliculata)是首批列入我国外来入侵物种黑名单的生物。本文采用干旱后复水方式喂养福寿螺48 d,研究间歇性干旱处理下福寿螺摄食生长、肝胰脏抗氧化能力及胃消化酶活力变化。结果显示:间歇性干旱能够显著降低福寿螺的体重增长率,对存活率影响不明显;福寿螺在恢复摄食期间出现部分补偿生长现象,主要是通过复水后增大摄食量来实现的,当间歇性干旱时间间隔延长到8 d时,则是通过增大摄食量和提高食物转化率的双重作用来实现的;除间隔4 d间歇性干旱下SOD酶活力以外,福寿螺在短间隔(1~4 d)间歇性干旱下SOD和CAT活力均出现不同程度的增加,而MDA含量明显降低;在长间隔(8 d)间歇性干旱下,SOD和CAT活力均低于对照组,且MDA含量与对照组无差别;间歇性干旱降低了福寿螺肝胰脏中GST和GSH-PX活性,对GSH含量无明显影响;除间隔1 d的处理组胃脂肪酶外,间歇性干旱能够不同程度地提高福寿螺胃淀粉酶和胃脂肪酶活力。总体而言,在间歇性干旱胁迫下,福寿螺可通过提高摄食率、食物转化率、抗氧化能力、消化酶活性等,在一定程度上来缓解胁迫带来的影响,但间歇性干旱能够明显抑制福寿螺的正常生长,表明通过农田水位交替性变化可在一定程度上防控福寿螺的暴发与危害。  相似文献   

11.
Summary The influence of the partial pressure of oxygen on denitrification and aerobic respiration was investigated at defined P02 values in a mull rendzina soil. The highest denitrification and respiration rates obtained in remoistened, glucose- and nitrate-amended soil were 43 1 N20 h–1g–1 soil and 130 1 O2 h–1g–1 soil, respectively. At -55 kPa matric water potential, corresponding to 40% water saturation, N20 was produced only below P02 40 hPa. The K m, for O2 was 3.0 x 106 M. Formation of N2O and consumption of O2 occurred simultaneously with half maximum rates at P02 6.7–13.3 hPa. Nitrite accumulated in soil below 40 hPa and increased with decreasing pO2. The upper threshold for N20 formation in amended soil was P02 33–40 hPa (39-47 M O2).  相似文献   

12.
陕西关中地区参考作物蒸发蒸腾量变化及原因   总被引:7,自引:2,他引:7  
根据关中地区30个气象站41年的气象资料,采用FAO推荐的Penman-Monteith公式计算参考作物蒸发蒸腾量(ET0),分析了陕西关中地区ET0的变化及原因,结果表明,从长期来看,关中地区ET0在减少趋势的基础上表现出周期性变化,从阶段性来看,1980年之前和之后则主要表现为增加趋势。关中地区年内ET0的最大值在1980年前主要出现在6月,1980年以后则主要出现在6月和7月,且以7月为多;1980年后5~8月ET0所占比值在减少,但仍在全年中占50%以上。关中地区平均气温、最高气温和最低气温表现为增加趋势,风速、日照时数和相对湿度表现为减少趋势。关中地区ET0与平均气温、最高气温和最低气温表现为不显著正相关,与风速和日照时数表现为显著正相关,与水汽压表现为显著负相关,与年降水量表现为不显著负相关,近一半地区的ET0与年蒸发量显著正相关。对关中地区ET0影响显著的气象因子的顺序为:风速〉日照时数〉水汽压〉年蒸发量。即风速和日照时数的减少趋势是引起关中地区ET0降低趋势的主要原因。  相似文献   

13.
Although phosphate-solubilizing microorganisms play a positive role in plant growth, their role in plant growth and root nodulation in combination with Rhizobium and Enterobacter has not been fully elucidated. Furthermore, only information exists regarding the effect of inoculation at successive stages of nodulation. The present study aimed to monitor the changes in the production of Indole acetic acid (IAA) and Gibberellin (GA3) in the roots of soybean during and after nodulation (25 DAS and 40 DAS) and in the rhizosphere soil following inoculation with two different strains of phosphate-solubilizing microorganisms (PSM I, strain CA 18 and PSM II, strain 54RB), Enterobacter strain A and BradyRhizobium Japonicum strain Tal 377. The effects of inoculation on the available NO?3, K, and P content of soil were studied at harvest. Beneficial effects of inoculation with Rhizobium, Enterobacter, and PSM I and II were obtained in all growth parameters of soybean. Co-inoculation resulted in maximum increase in IAA and GA content, plant biomass, root nodulation, number, weight and length of pods as compared with control (non-inoculated) and single-inoculation plants. The soil of the inoculated plants also showed higher IAA and GA content over.  相似文献   

14.
北亚热带次生毛竹林冠生态水文效应及其影响因素分析   总被引:4,自引:0,他引:4  
对长江三角洲森林生态定位站北亚热带次生毛竹林(Phyllostachys edulis)林冠水文作用进行了观测研究.结果表明:(1)毛竹林林冠截留量、透流量、树干茎流量变化范围分别为0.21~4.55 mm,0~105.88 mm,0~2.8 mm;截留率、透流率、树干茎流率变化范围分别为1.3%~100%,0~96.3%,0~11.8%;年截留量、透流量、干流量分别为171.7,864.78,74.3 mm;年截留率、透流率、树干茎流率分别为15.5%,77.9%,6.7%.(2)毛竹林林冠截留量(率)、透流量(率)、树干茎流量(率)均与降雨量、降雨强度存在着不同程度的相关性,降雨特征对毛竹林林冠分配降雨的各环节影响最大.(3)不同雨量级、雨强条件下,毛竹林分配降雨效果差异显著.  相似文献   

15.
The rose of an isolate of the arbuscular mycorrhizal (AM) fungusGlomus mosseae in the protection ofMedicago sativa (+Rhizobium meliloti) against salt stress induced by the addition of increasing levels of soluble salts was studied. The interactions between soluble P in soil (four levels), mycorrhizal inoculum and degree of salinity in relation to plant growth, nutrition and infective parameters were evaluated. Salt stress was induced by sequential irrigation with saline water having four concentrations of three salts (NaCl, CaCl2, and MgCl2).15N-labelled ammonium sulphate was added to provide a quantitative estimate of N2 fixation under moderate to high salinity levels. N and P concentration and nodule formation increased with the amount of plant-available P or mycorrhizal inoculum in the soil and generally declined as the salinity in the solution culture increased from a moderate to a high level. The mycorrhizal inoculation protected the plants from salt stress more efficiently than any amount of plant-available P in soil, particularly at the highest salinity level applied (43.5 dS m–1). Mycorrhizal inoculation matched the effect on dry matter and nutrition of the addition in the soil of 150 mg P kg–1. Nevertheless the highest saline solution assayed (43.5 dS m–1) affected more severely plants supplemented with phosphorus than those with the addition of mycorrhizal inoculum. Such a saline-depressing effect was 1.5 (biomass), 1.4 (N) and 1.5 (P) times higher in plants supplied with soluble phosphate than with AM inoculum. Mechanisms beyond those mediated by P must be involved in the AM-protectioe effect against salinity. The15N methodology used allowed the determination of N2 fixation as influenced by different P applications compared to mycorrhizal inoculation. A lack of correlation between nodule formation and function (N2 fixation) was evidenced in mycorrhizal-inoculated plants. In spite of the reduced activity per nodule in mycorrhizal-inoculated In spite of the reduced activity per nodule in mycorrhizal-inoculated plants, the N contents determined indicated the highest acquisition of N occurred in plants with the symbiotic status. Moreover, N and P uptake increased while Ca and Mg decreased in AM-inoculated plants. Thus P/Ca ratios and cation/anion balance in general were altered in mycorrhizal treatments. This study therefore confirms previous findings that AM-colonized plants have optional and alternative mechanisms available to satisfy their nutritive requirements and to maintain their physiological status in stress situations and in disturbed ecosystems.  相似文献   

16.
We examined the effects of conifers on the forms of P in low-fertility tussock grassland soils using 31P nuclear magnetic resonance (NMR) and soil P fractionation. Results from field and glasshouse experiments clearly demonstrated that conifers enhanced the mineralization of labile (and to a lesser extent more resistant) forms of soil organic P which, in turn, increased amounts of labile inorganic P in the soil. These findings have important implications for P availability and long-term sustainable management of grassland soils in New Zealand.  相似文献   

17.
Continuous half-hourly measurements of soil (Rs) and bole respiration (Rb), as well as whole-ecosystem CO2 exchange, were made with a non steady-state automated chamber system and with the eddy covariance (EC) technique, respectively, in a mature trembling aspen stand between January 2001 and December 2003. Our main objective was to investigate the influence of long-term variations of environmental and biological variables on component-specific and whole-ecosystem respiration (Re) processes. During the study period, the stand was exposed to severe drought conditions that affected much of the western plains of North America. Over the 3 years, daily mean Rs varied from a minimum of 0.1 μmol m−2 s−1 during winter to a maximum of 9.2 μmol m−2 s−1 in mid-summer. Seasonal variations of Rs were highly correlated with variations of soil temperature (Ts) and water content (θ) in the surface soil layers. Both variables explained 96, 95 and 90% of the variance in daily mean Rs from 2001 to 2003. Aspen daily mean Rb varied from negligible during winter to a maximum of 2.5 μmol m−2 bark s−1 (2.2 μmol m−2 ground s−1) during the growing season. Maximum Rb occurred at the end of the aspen radial growth increment and leaf emergence period during each year. This was 2 months before the peak in bole temperature (Tb) in 2001 and 2003. Nonetheless, Rb was highly correlated with Tb and this variable explained 77, 87 and 62% of the variance in Rb in the respective years. Partitioning of Rb between its maintenance (Rbm) and growth (Rbg) components using the mature tissue method showed that daily mean Rbg occurred at the same time as aspen radial growth increment during each growing season. This method led, however, to systematic over- and underestimations of Rbm and Rbg, respectively, during each year. Annual totals of Rs, Rb and estimated foliage respiration (Rf) from hazelnut and aspen trees were, on average, 829, 159 and 202 g C m−2 year−1, respectively, over the 3 years. These totals corresponded to 70, 14 and 16%, respectively, of scaled-up respiration estimates of Re from chamber measurements. Scaled Re estimates were 25% higher (1190 g C m−2 year−1) than the annual totals of Re obtained from EC (949 g C m−2 year−1). The independent effects of temperature and drought on annual totals of Re and its components were difficult to separate because the two variables co-varied during the 3 years. However, recalculation of annual totals of Rs to remove the limitations imposed by low θ, suggests that drought played a more important role than temperature in explaining interannual variations of Rs and Re.  相似文献   

18.
Summary Inoculated and non-inoculated seedlings of Zea mays were grown in agricultural soils under aseptic and non-sterile conditions. Acetylene reduction activity and microbial counts were determined after 7 and 30 days of growth. Irrespective of the soil type Azotobacter spp. were commonly isolated under maize cultivation. Inoculation of agricultural soils with a suspension of A. chroococcum led to an increase in Azotobacter numbers, although this effect diminished with time. Nitrogenase activity was detected on maize roots and increased in response to the inoculation with A. chroococcum, showing that this associative growth could be of primary importance for the plant. The results of assays for acetylene reduction activity indicated that the nitrogenase activity was associated only with the root systems.  相似文献   

19.
水稻与慈姑间作栽培对水稻病虫害和产量的影响   总被引:1,自引:1,他引:1  
合理的间套作种植能够显著降低病虫害的发生,但已有相关研究主要集中在旱地作物种类或旱稻上,而水田环境下水稻间作体系的作物搭配及种植模式研究目前还十分缺乏。本文通过田间对比试验研究了水稻与慈姑间作栽培对水稻病虫害、微环境以及水稻产量的影响效应。结果表明,间作栽培模式对稻飞虱和稻纵卷叶螟无显著的防控效果。但在拔节期和抽穗期期间,间作栽培模式下水稻纹枯病病丛率分别比单作处理低64.3%和88.2%,稻瘟病病叶率在灌浆期和乳熟期显著低于单作,表明间作栽培模式显著降低了水稻稻瘟病和纹枯病的发生。间作栽培模式下水稻叶片POD、SOD和CAT酶活性与单作模式相比出现了不同程度降低,单作模式下水稻较高的病害发生程度可能是导致叶片保护酶活性增高的原因;间作栽培在早午时段降低了水稻叶片表面的空气湿度,且水稻叶片光合有效辐射强度在多数时段均高于单作栽培,说明间作栽培模式改善了稻田小气候环境,有利于降低病菌的滋生和传播;光合气体交换日进程测定结果表明,灌浆期间水稻/慈姑间作栽培模式下水稻叶片净光合速率、蒸腾速率以及气孔导度在日间大部分时段均高于单作水稻,说明间作栽培模式提高了水稻的群体光能利用率以及群体光合效率;间作栽培模式下的土地当量比为1.17,表明水稻与慈姑间作栽培模式不仅能有效控制水稻病虫害的发生,同时能起到良好的增产效果。  相似文献   

20.
ABSTRACT

We estimate the electrical conductivity of saturated soil paste extract (ECe) from electrical conductivity of a 1:5 soil-water dilution ratio (EC1:5) in Northeastern Thailand. Soil samples of various textures and salinity collected from Sakhon Nakhon basin were used to develop multiple regression models, from which the linear model was chosen and was validated on soil samples from the Khorat basin. Comparison with previous models indicated that most linear models gave a good fit, but the non-linear models either over or underestimated the measured values. The models performed very well for low values of ECe (<5 dS m?1), while the prediction errors increased significantly for ECe levels >35 dS m?1. The present model performed well at various ECe levels and can be used to predict salinity levels for soils weathered from salt deposits in sedimentary rocks with similar rock formation in countries like Malaysia, Vietnam, Cambodia, and Laos.  相似文献   

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