Preliminary Results: The Advantages of Low-Density Lipoproteins for the Cryopreservation of Equine Semen

The aim of this study was to determine the best concentration of low-density lipoproteins (LDL) in a semen extender to improve the percentage of motile spermatozoa in equine sperm after freezing and thawing in comparison with standard extenders. Ten extenders were compared: 1 with 2% egg yolk (EY), 8 with different concentrations of LDL (0.25%, 0.50%, 0.75%, 1%, 2%, 3%, 4%, and 5%), and INRA 96; all of the extenders contained 2.5% glycerol. Fourteen ejaculates were collected from four different stallions. The first dilution was made with equal parts at +37°C, centrifuged (600 × g/10 min), and resuspended in the corresponding extenders to obtain a final concentration of 100 × 10⁶ spermatozoa/ml. The resulting mixture was cooled to 4°C over 1 hour, packed into four 0.5-ml straws, and left for a further 30 minutes at +4°C. Finally, the straws were frozen in nitrogen vapors 4 cm over liquid nitrogen for 10 minutes before being immersed in liquid nitrogen at −196°C and stored. Two straws per extender and per ejaculate were thawed in a water bath at +37°C for 30 seconds. The contents of each straw were recovered into a cryotube and placed in a water bath at +37°C for 10 minutes before being examined with an image analyzer. The best post-thaw motility results were obtained with the extenders made with 0.5%, 2%, and 3% LDL and with the control extender made with egg yolk; no significant difference was observed between these extenders. The last two straws were thawed to perform four sperm function tests. The hypo-osmotic test was used to assess the integrity of the plasma membrane; the 2% and 3% LDL treatments were the most suitable and were comparable to that with whole egg yolk for protecting stallion sperm during cryopreservation (32.3%, 32.4%, and 31.3%, respectively). The Pisum sativum agglutinin-fluorescein isothiocyanate test was used to verify the integrity of the acrosomes; the best results were obtained with the 0.5%, 0.75%, and 3% LDL and INRA96 extenders; no significant differences were observed among the 85.8%, 85.0%, 84.7%, and 84.8% extenders. The acridine orange test was used to assess DNA integrity; there were no significant differences among the various extenders: the DNA was preserved in 98% of the spermatozoa. Finally, spermatozoal morphology was examined using Spermac stain; 78% of the spermatozoa did not present any anomalies in the 0.25% and 2% LDL extenders. In conclusion, the 2% LDL extender gave the best post-thaw percentage of motile spermatozoa. The results of the sperm function test were also superior for this extender.     

燕麦种质抗坚黑穗病鉴定与评价

为明确不同燕麦种质抗坚黑穗病（Ustilago segetum var. avenae）的差异和探讨形成燕麦抗坚黑穗病鉴定技术规范,于2013-2014年采用菌粉拌种和菌土覆盖的接种方法对135份燕麦种质进行坚黑穗病的田间抗性鉴定,结果表明,在菌粉拌种条件下,64份裸燕麦中,12份免疫,6份高抗,1份抗,19份中感,16份感,10份高感,分别占鉴定裸燕麦的18.8%,9.4%,1.6%,29.7%,25.0%,15.6%;71份皮燕麦中,45份免疫,22份高抗,2份抗,2份中感,分别占鉴定皮燕麦的63.4%,31.0%,2.8%,2.8%。在菌土覆盖条件下,64份裸燕麦中,17份免疫,8份高抗,13份抗,22份中感,3份感,1份高感,分别占鉴定裸燕麦的26.6%,12.5%,20.3%,34.4%,4.7%,1.6%;71份皮燕麦中,56份免疫,13份高抗,1份抗,1份中感,分别占鉴定皮燕麦的78.9%,18.3%,1.4%,1.4%;在2种接种方法下,62份材料抗性完全一致;10份材料在菌粉拌种接种方法的抗病性低于菌土覆盖的抗病性;菌粉拌种较菌土覆盖易使63个燕麦品种的发病率增加,使其抗病性下降,说明菌粉拌种接种方法明显优于菌土覆盖。试验中获得的这些有效抗病种质,可为品种的合理布局和更替提供科学依据,同时可为育种部门提供核心抗源。     

鄂西北地区规模化鸡场H9亚型禽流感抗体水平监测

本试验旨在了解鄂西北地区规模化鸡场鸡禽流感(H9亚型,下同)免疫水平。2007年8月—2010年5月对鄂西北地区65个鸡场接种H9禽流感疫苗20 d后的鸡随机采集血样2311份,应用ELISA方法对血清的禽流感抗体水平进行监测调查。调查结果表明,所检测的2311份血样中,2007年检测11家鸡场共135份血样,104份阳性,阳性率77.0%,强阳性83份,强阳性率61.5%。其中,免疫合格鸡场4家,占受检鸡场36.4%;2008年检测25家鸡场共985份血样,883份阳性,阳性率89.6%,强阳性732份,强阳性率74.3%。其中,免疫合格鸡场21家,占受检鸡场84.0%;2009年检测16家鸡场共433份血样,348份阳性,阳性率80.4%,强阳性277份,强阳性率64.0%。其中,免疫合格鸡场12家,占受检鸡场75%。2010年检测13家鸡场共758份血样,638份阳性,阳性率84.2%,强阳性575份,强阳性率75.6%。其中,免疫合格鸡场11家,占受检鸡场84.6%。2007年禽流感的血清抗体水平较低,相比较,2008—2010年有了明显增加。受检的规模化鸡场仅部分能达到保护水平,且各鸡场的免疫合格鸡比例方面存在较大差异。     

4种有机溶剂对Vero细胞活力和弓形虫增殖的影响

本研究旨在筛选低细胞毒性和低弓形虫毒性的有机溶剂。Vero细胞在96孔板培养12 h后，将培养基更换为200μL分别含0、0.1%、0.3%、0.5%、1%、3%、5%、10%（V/V）的二甲基亚砜（DMSO）、二甲基甲酰胺、乙醇和甲醇的完全培养基，同时设立空白对照孔，继续培养12和24 h后，采用噻唑蓝（MTT）法评价4种有机溶剂对Vero细胞增殖活力的影响，筛选出对宿主细胞毒性最小的有机溶剂并确定其安全浓度；弓形虫接种Vero细胞12 h后，弃上清后分别加入含有0、0.1%、0.3%、0.5%、1%、3%（V/V）的DMSO、二甲基甲酰胺、乙醇和甲醇的完全培养基120μL，接种36 h后判定各组溶剂对弓形虫增殖的影响，计数感染细胞数并计算相对感染率，筛选出对弓形虫增殖影响最小的有机溶剂及其安全浓度。结果显示，对于Vero细胞，DMSO、二甲基甲酰胺、甲醇和乙醇分别在其体积分数低于1%、0.1%、3%和1%时无明显毒性；对于弓形虫，DMSO、二甲基甲酰胺、甲醇和乙醇对弓形虫增殖的安全添加浓度分别为1%、0.1%、1%和0.1%。本研究表明，DMSO、甲醇对Vero细胞、弓形虫的毒性较小，可作为水溶性较差药物进行抗弓形虫筛选时优先选择的有机溶剂。     

天祝夏玛林区蝶类群落特征

2008-2010年连续3年7-9月,对天祝夏玛林区的蝶类进行了研究。采集到蝶类标本2 000余号,经鉴定整理出蝶类71种及亚种,隶属8科50属。其中粉蝶科有9属20种,占总种数的28.2%,为优势类群;蛱蝶科有15属15种,占总种数的21.1%,眼蝶科13属14种,占总种数的19.7%,为次优势类群;绢蝶科1属9种,占总种数的12.7%;灰蝶科8属8种,占总种数的11.3%,为常见类群;弄蝶科2属2种,占总种数的2.8%,凤蝶科1属2种,占总种数的2.8%,蚬蝶科1属1种,占总种数的1.4%,为罕见类群。单种属有43个,占总属的86%,包含的种占总种数的61%;多种属有7个,占总属的14%,包含的种有28种,占总种数的39%;属种比值系数为0.86。结果表明,该地区蝶类以单种属为主。     

日粮蛋白质水平对断奶仔猪氮代谢的影响

试验研究了日粮不同蛋白质水平对断奶仔猪氮代谢的影响。试验采用完全随机设计,将72头35日龄断奶的仔猪分为4组,每组3个重复,每个重复6头。4个组分别饲喂24%、22%、20%、18%蛋白水平的日粮。结果表明:粪氮排泄随日粮蛋白水平的升高而增加,24%蛋白组粪氮排泄量显著高于其它3组(P<0.05),尿氮变化不显著;22%、20%、18%蛋白组氮表观消化率无显著差异,24%蛋白组氮表观消化率显著低于其它3组(P<0.05);氮代谢率随蛋白水平上升而下降;血清总蛋白浓度与日粮蛋白水平无显著的相关性;血清尿素氮浓度随日粮蛋白水平的降低而降低。总之,考虑到仔猪生长和氮排出,仔猪日粮蛋白水平应以20%~22%为好,18%的日粮蛋白水平影响仔猪日沉积氮量。     

转高赖氨酸基因稻谷替代玉米对肉鸡生产、屠宰性能的影响

试验采用不同比例(40%、100%)的转高赖氨酸基因稻谷替代玉米饲喂AA肉鸡,以研究转高赖氨酸基因稻谷替代玉米的效果及可行性。结果表明:21日龄,40%组平均体增重比对照组显著提高6.86%;40%组和100%组的料肉比比对照组分别低7.3%和10.1%;试验组与对照组的胸肌率差异显著,但两试验组之间差异不显著,40%组、100%组分别比对照组显著提高20.9%和14.4%;全净膛率、腿肌率和腹脂率均差异不显著。42日龄,40%组和100%组与对照组的平均体增重均差异不显著,但两试验组之间差异显著,100%组的平均体增重比40%组提高12.8%;料肉比相当;胸肌率三组间差异显著,40%组、100%组分别比对照组显著提高11.4%和22.5%,100%组比40%组显著提高10.0%;40%组腿肌率比对照组显著提高12.5%,而100%组与40%组和对照组均差异不显著;40%组的腹脂率比100%组、对照组分别显著提高38.6%和30.8%,全净膛重、全净膛率差异不显著。试验得出,用40%和100%转高赖氨酸基因稻谷替代玉米饲喂肉仔鸡无不良影响。     

Effects of Fermented Atractylodes chinensis on Serum Immune and Biochemical Indexes of Weaned Piglets

The aim of this experiment was conducted to study the effects of fermented Atractylodes chinensis on serum immune and biochemical indexes of weaned piglets. Ninety-six Landrace ×Large White weaned piglets with weaning weight (6.0±0.5) kg were randomly allocated to 6 groups with 4 replicates per group and 4 weaned piglets per replicate. The weaned piglets were subjected to the following 6 treatments for 28 days:Control group was fed with the basal diet,groups Ⅰ and Ⅱ were fed with antibiotics and 0.2% unfermented Atractylodes chinensis in basal diet,while groups Ⅲ,Ⅳ and Ⅴ were supplemented 0.1%,0.2% and 0.3% fermentated Atractylodes chinensis in basal diet,respectively. The results showed that the immune and biochemical index of weaned piglets in fermented Atractylodes chinensis group had different degrees of improvement compared with control group and group Ⅰ.In the whole experimental stage,comparing with control group,the serum IgA,IgG,IgM,TP and ALB of weaned piglets in group Ⅳ were increased 10.5%,10.0%,7.8%,21.0% and 15.6%,respectively,and the difference were significant (P < 0.05),and serum ALT content was significantly reduced 20.4% (P < 0.05).Compared with group Ⅰ,the serum IgA,IgG,IgM and TP of weaned piglets in group Ⅳ were increased 7.1%,3.8%,3.8% and 12.6%, respectively,the difference were significant (P < 0.05),while the serum ALB, AST and BUN of group Ⅳ were increased 1.0%, 9.5% and 15.0%, and the difference were not significant (P > 0.05), and serum ALT content was reduced 9.4% compared with the antibiotics group (P > 0.05).     

复合微生物菌剂在猪粪无害化处理中的应用

为了研究微生物菌剂对粪便恶臭组分及微生物菌群的影响,选取猪粪和玉米秸秆作为堆肥原料进行28 d的堆肥试验.试验分为5组,A、B和C组分别用玉米秸秆调节堆体水分为40％、50％和60％,D和E组不加玉米秸秆,A、B、C和D组分别接种微生物菌剂,E组不添加菌剂.结果 显示,B组堆体的高温持续时间最长,粪便腐熟效果最好.与E...     

不同处理玉米秸秆营养成分及其肉鸡表观代谢能评定

本试验旨在评价不同处理玉米秸秆营养成分及其肉鸡表观代谢能。选择40只42日龄健康AA商品肉鸡,随机分为8个处理,每个处理5只鸡。直接法分为3个处理组,分别为强饲15 g 100%未处理秸秆、50 g 100%糖化秸秆、50 g 100%酵母发酵秸秆;差量法分为5个处理组,即基础饲粮组和试验组。试验组分别在基础饲粮中添加4%、8%、12%糖化玉米秸秆和8%酵母发酵秸秆替代等量玉米。通过饲养试验验证差量法和直接法对糖化玉米秸秆代谢能测定的准确性。结果表明:与未处理玉米秸秆相比,糖化秸秆中还原糖和钙含量分别增加8.08倍和9.31倍(P<0.05);与糖化玉米秸秆相比,酵母发酵秸秆中还原糖降低78.69%(P<0.05),真蛋白提高29.64%(P<0.05),总氨基酸提高41.01%(P<0.05);通过直接法得出糖化玉米秸秆和酵母发酵玉米秸秆的表观代谢能分别为4.85、4.75 MJ/kg;差量法测定糖化玉米秸秆在饲粮中添加4%、8%和12%时表观代谢能分别为15.85、15.81、14.38 MJ/kg,高于酵母菌发酵玉米秸秆(P<0.05);通过饲养试验验证,差量法测定的表观代谢能结果可信。结果显示,糖化玉米秸秆代谢能高于酵母发酵秸秆和玉米,可以作为肉鸡的饲料能量原料。     

发酵北苍术对断奶仔猪血清免疫指标和生化指标的影响

试验旨在研究发酵北苍术对断奶仔猪血清免疫指标和生化指标的影响。选取96头体况相近、断奶重为（6.0±0.5）kg的长白猪×大白猪二元杂交仔猪,随机分为6组,每组4个重复,每个重复4头猪。对照组饲喂基础日粮,Ⅰ组为抗生素组,在基础日粮中添加杆菌肽锌＋硫酸黏杆菌素,Ⅱ组在基础日粮中添加0.2%未发酵北苍术,Ⅲ、Ⅳ、Ⅴ组分别在基础日粮中添加0.1%、0.2%、0.3%发酵北苍术,试验期为28 d。结果显示,日粮中添加发酵北苍术组的断奶仔猪的血清各项免疫指标和生化指标较对照组及Ⅰ组均有不同程度的改善,其中Ⅳ组的效果最好。在整个试验阶段（断奶后28 d）,与对照组相比,Ⅳ组断奶仔猪血清中IgA、IgG、IgM、TP和ALB含量分别提高了10.5%、10.0%、7.8%、21.0%和15.6%,差异均显著（P < 0.05）,血清中ALT含量与对照组相比显著降低了20.4%（P < 0.05）;与Ⅰ组相比,Ⅳ组断奶仔猪血清中IgA、IgG、IgM、TP分别提高了7.1%、3.8%、3.8%、12.6%,差异均显著（P < 0.05）,而ALB、AST和BUN含量分别提高了1.0%、9.5%和15.0%,差异均不显著（P > 0.05）,血清中ALT含量与Ⅰ组相比降低了9.4%（P > 0.05）。     

奶牛场生产母牛产后瘫痪发病率调查及典型产后瘫痪病例诊治

奶牛产后瘫痪是母牛分娩前后突然发生的一种严重的代谢性疾病。本文统计了伊犁某荷斯坦奶牛场2015年4月至2018年3月1 200头分娩奶牛生产瘫痪发病情况,分娩奶牛发生产后瘫痪104头,产后瘫痪平均发病率为8.65%,以1月、12月、2月发病率较高,为11.11%～13.89%;而4月、9月、10月发病率低,为6.61%～6.71%。高产奶牛在5胎以上产后瘫痪发病率达10.30%～11.79%,而第2胎发病率很低,在3.59%。本文着重对1例典型病例发病原因、临床症状及发病特点进行详细论述,采取补钙、补糖及结合乳房送风治疗效果明显,从而得出了一些治疗预防的方法,为减少养殖场奶牛产后瘫痪发病率提供了参考。     

不同添加水平多聚甲醛对绵羊瘤胃微生物数量及消化酶活性的影响

将5只体重(55.6±3.2)kg、2～3岁、装置了永久性瘤胃瘘管的小尾寒羊公羊,按5×5拉丁方设计,在饲喂相同日粮条件下,在5期试验中分别添喂0、150、300、450和600 mg/kg多聚甲醛,测定瘤胃液pH,氨态氮及挥发性脂肪酸浓度,内纤维素酶、外纤维素酶、纤维二糖酶、木聚糖酶、果胶酶、淀粉酶和蛋白酶活性,并进行原虫、细菌显微镜分类和真菌PCR计数,以研究不同添加剂量多聚甲醛对绵羊瘤胃微生物及消化酶活性的影响。结果表明,与对照组相比,添喂150、300、450和600 mg/kg多聚甲醛,绵羊的干物质自由采食量分别增加2.3%(P>0.05)、22.1%(P<0.01)、7.5%(P<0.01)和-6.6%(P<0.01);瘤胃液原虫总数分别减少14.2%、62.5%、72.6%和85.3%(P<0.01);而细菌总数分别增加35.8%、93.8%、-6.8%和-24.2%(P<0.01);真菌拷贝数分别增加2.0%(P>0.05)、5.4%(P>0.05)、14.3%(P<0.01)和26.8%(P<0.01);氨态氮分别降低9.0%、12.0%、14.9%和20.9%(P<0.01);挥发性脂肪酸分别提高2.4%(P>0.05)、-0.3%(P>0.05)、-1.3%(P>0.05)和-10.3%(P<0.01);内纤维素酶活性分别提高6.5%、22.4%、-10.2%和-19.2%(P<0.01);外纤维素酶活性分别提高3.4%(P>0.05)、17.5%(P<0.01)、-18.0%(P<0.01)和-25.7%(P<0.01);纤维二糖酶活性分别提高8.3%、23.7%、-24.9%和-31.8%(P<0.01);淀粉酶活性分别提高2.8%、8.6%、16.0%和23.3%(P<0.01);果胶酶活性分别降低11.8%、12.1%、11.2%和11.1%(P<0.01);蛋白酶活性分别降低10.9%、30.4%、49.6%和56.2%(P<0.01);而木聚糖酶活性没有显著变化(P>0.05)。综合试验结果,多聚甲醛添喂剂量为300 mg/kg时,绵羊的自由采食量最高,此时瘤胃液原虫数量减少而细菌总数最多、纤维素酶活性最高。     

牛源耐甲氧西林金黄色葡萄球菌的分离鉴定

从奶牛分离得到耐甲氧西林金黄色葡萄球菌（methieillin—resistant Staphylococcus aureus,MRSA）。按常规方法复苏菌株,用血浆凝固酶试验鉴定SA。用KB法、琼脂筛选法、生化鉴定法（VITEK32）、聚合酶链反应等鉴定MRSA。常规分离鏊定的葡萄球菌468株,其中凝固酶阴性葡萄球菌（coagulase negative Staphylococcus,CNS）361株,检出率为77．149,6,金黄色葡萄球菌（Staphylococcus aureus,SA）105株,检出率为22．44％,MRSA36株检出率为34．29％,2株未定型,占0．42％。而MRSA在乳房炎奶样中检出率高达34．29％,表明新疆兵团部分垦区牛场MRSA已经呈蔓延趋势。     

Performance of Commercial Laying Hens When Fed Diets with Various Sources of Energy

Two experiments were conducted to determine the influence of adding various fat sources to the diet of the aged (76 to 84 wk) commercial laying hen. In experiment 1, corn-soybean meal diets were fed with 0, 2 and 4% corn oil. A diet containing 13.85% wheat bran was fed with and without 4% corn oil. A sixth diet contained 2% corn oil and 26.21% sugar. No difference in egg production among the 6 treatments was observed. The addition of 4% corn oil produced a nonsignificant increase in egg weight. The corn-soybean meal diet containing no corn oil also was used as the control in experiment 2. Seven treatment diets were fed with 4% fat from various sources. No difference in egg production among the 8 treatments was observed. Egg weight was higher when the diet contained added fat except when tallow was added.     

新疆喀什地区安格斯犊牛腹泻主要病毒性病原调查及牛诺如病毒全基因序列分析

【目的】调查新疆喀什地区安格斯犊牛腹泻主要病毒性病原流行情况及牛诺如病毒(Bovine norovirus, BNoV)全基因组遗传关系。【方法】采用RT-PCR技术分别对2021年不同季节在喀什4个牛场采集的363份犊牛腹泻粪便样品进行牛轮状病毒(Bovine rotavirus, BRV)、牛冠状病毒(Bovine coronavirus, BCoV)、牛病毒性腹泻病毒(Bovine viral diarrhea virus, BVDV)和BNoV 4种病毒性病原检测,并对扩增获得的BNoV全基因序列进行分析。【结果】BCoV和BNoV检出率较高,分别为36.09%(131/363)和25.07%(91/363),混合感染中BCoV+BNoV检出数量最多,阳性率为44.00%(22/50)。对喀什地区4个场区进行检测,其中A场主要检测出BCoV和BNoV,检出率分别为43.52%(47/108)和32.41%(35/108);B场主要检测到BCoV,检出率为52.21%(71/136);C、D场病原检出率普遍较低。秋、冬季检出量最多的病毒分别为BNoV和BCoV,检出率分别为66....     

添加尿素和糖蜜对甘蔗尾打捆青贮品质的影响

试验采用单因子设计研究不同水平的尿素、糖蜜及糖蜜与尿素混合处理对甘蔗尾打捆青贮品质的影响。试验共分5组,其中1组为对照组,甘蔗尾经揉搓后直接青贮,2~5组为试验组分别添加0.6%尿素、1.0%的尿素、5%糖蜜和0.6%尿素+5%糖蜜,每个处理3个重复,青贮期90 d。试验结果表明,添加0.6%、1%的尿素及0.6%尿素+5%糖蜜均能显著提高青贮饲料的粗蛋白质含量（P<0.05）,但3个组间差异不显著（P>0.05）;单独加尿素的两组青贮料pH较对照组和其它试验组高,外观品质有所下降;添加5%的糖蜜和0.6%尿素+5%糖蜜均能显著降低青贮料的粗纤维含量,增加可溶性糖的含量,青贮料的pH及外观品质较好;综合营养成分和外观品质, 甘蔗尾用尿素与糖蜜混合处理其青贮料的品质最好。     

Residue Analysis of Five Kinds of Penicillins in Eggs by High Performance Liquid Chromatography-Ultraviolet Detection

This study was aimed to develop a method for simultaneous determination of five kinds of penicillins in eggs by high performance liquid chromatography-ultraviolet detection (HPLC-UVD). The eggs samples were extracted by acetonitrile,degreased by n-hexane,concentrated by rotary evaporation,derivatization,and then was separated by HPLC. The results showed that the calibration curves of five kinds of penicillins were linear correlated in the concentration ranges of 10 to 1 000 μg/kg with the correlation coefficients >0.999,which the limits of detection and quantification were 1.5 μg/kg (S/N=3) and 5.0 μg/kg (S/N=10),respectively. The average recoveries were 62.04% to 93.13%,the relative standard deviations were 3.91% to 13.69%.And the intra-day and inter-day RSDs were 3.98% to 14.71% and 4.26% to 12.71%,respectively. The developed method was suitable for detecting five kinds of penicillins residues in eggs with high repeatability and sensitivity.     

Effects of Different Types of N-carbamylglutamate on the Growth Performance,Serum Biochemical Indexes and Fat Distribution of Tan Sheep

The objective of the present experiment was to determine the effect of different types of N-carbamoylglutamate on growth performance,serum biochemical indexes and fat distribution of Tan sheep. Sixty male Tan lambs with average weight of (20.14±0.31)kg and similar age were randomly assigned three groups with five replications per group and 4 lambs per replication. The lambs in control group were fed basal diet, while lambs in test groups were fed diets containing 0.1% NCG and 0.1% RP-NCG,respectively. The results showed that:① Comparing with control group,the ADG was significantly improved (P<0.05) and F/G was significantly decreased (P<0.05) when 0.1% NCG or 0.1% RP-NCG were supplemented,while there was no significant difference between two experimental groups (P>0.05).② Dietary supplemented with 0.1% NCG or 0.1% RP-NCG significantly decreased the serum urea nitrogen (P<0.05),but there were no significant differences in serum total protein,creatinine content and creatine kinase activity (P>0.05).③ The ration of meat weight to carcass weight of two experimental groups was significantly improved (P<0.05).As 0.1% NCG or 0.1% RP-NCG were supplemented in the diets, the content of subcutaneous fat and GR value were significantly decreased (P<0.05),and the content of intramuscular fat (IMF) was significantly increased (P<0.05).In conclusion,dietary supplemented with 0.1% NCG or 0.1% RP-NCG could improve the growth performance and slaughter performance while there was no significant difference between NCG and RP-NCG.