副结核分枝杆菌MAP0862蛋白的表达及在间接ELISA中的初步应用

本研究的目的是探索副结核分枝杆菌特异性蛋白MAP0862在牛副结核病血清学诊断中的作用,建立牛副结核病特异性ELISA诊断方法。将通过原核表达获得的副结核特异性蛋白MAP0862纯化并定量后作为包被抗原,经过一系列条件优化后初步建立了牛副结核病间接ELISA诊断方法。使用牛副结核阳性血清、牛布病阳性血清、牛结核阳性血清、卡介苗免疫牛血清、牛大肠杆菌阳性血清以及健康阴性牛血清对该方法的验证表明其具有良好的特异性。使用该方法对300份临床血清进行检测,结果表明该方法与IDEXX副结核检测试剂盒的符合率为94.3%。基于副结核特异性蛋白质MAP0862建立的间接ELISA诊断方法能有效检测牛副结核病。     

动物疫病监测与免疫效果监测的联系和区别

正动物疫病监测与免疫效果监测都是通过实验室检测抗体水平来判定的,大部分往往是通过采取易感动物(免疫动物)血样,分离血清,通过实验室采取免疫抗体监测方法,通过测定抗体水平来进行判定,两者都是动物免疫抗体监测,极易造成混淆,特别口蹄疫无疫区建设评估方案要求:免疫效果监测抽样记录和相关检测报告与动物疫病监测采样单、检测报告两项内容,部分基层站认为是重复,是一样的,现将动物疫病监测与免疫效果监测的联     

口蹄疫O型LPB-ELISA免疫抗体高通量定性检测技术的建立

为提高偶蹄动物口蹄疫O型液相阻断酶联免疫吸附(LPB-ELISA)免疫抗体检测效率,优化动物疫情监测策略,提高动物疫病防控效能.结合基层一线对免疫质量评价实际需求及财力人力等因素,在该试验通用操作步骤基础上,特对操作步骤及96孔ELISA板检测样品布局进行了技术改进.改进后的1块ELISA板可检测样品80份,在检测通量...     

免疫诊断技术检测气管螨的新进展

酶联免疫吸附法(ELISA)是医学研究人员用于疾病诊断的一种技术。该法对诊断蜜蜂气管螨很有帮助。临床实验室常规使用该法检查人体血清以诊断受滋离,肝炎等疾病,但该法不仅局限于疾病的检查,而且能够用于其它多种物质的检测,如药物、激素、蛋白质及活体器官的分子成分。酶联免     

鸡病诊断的基本方法和程序

疾病监测是预防和控制疾病的重要手段。通过监测技术的实施，可以帮助了解疫病流行状况，掌握免疫时机，以能及时进行免疫，了解免疫效果。监测工作可以帮助诊断疾病，鉴定病原，为疾病的预防、控制和净化提供科学依据。检测技术还可用于环境污染状况调查及消毒效果检查。     

2020年山东省春季口蹄疫和高致病性禽流感免疫抗体检测

为了解山东省口蹄疫、高致病性禽流感两种重大动物疫病的免疫效果,按照山东省动物疫病监测计划,采集全省98个场(户)2909份血清样品,应用液相阻断ELISA和血凝抑制试验(HI)检测方法进行血清抗体检测.结果显示:O型口蹄疫抗体样品合格率为83.73％,A型口蹄疫为77.5％,高致病性禽流感H5亚型Re-11、H5亚型R...     

贵阳市规模化猪场主要疫病免疫程序的研究

为探索一套适合本地区规模化猪场主要疫病免疫程序,结合贵阳市科技局星火项目《优质安全猪肉生产监管技术集成与示范》,通过对贵阳市14个规模化养猪场血清学调查,针对性地制定了一套适合本地区规模养猪场主要疫病的免疫程序。1免疫程序实施前对猪群主要疫病的血清抗体调查1.1猪群中免疫疫病的血清抗体水平检测2010年4—6月采集贵阳市3个区(县)的14个规模猪场血清样本271份,采用ELISA(酶联免疫     

酶联免疫吸附试验的原理、操作方法及注意事项

酶联免疫吸附（ELISA）试验具有灵敏性、特异性高，且重复性好，检测速度快的特点，尤其适合于大批量血清样品的检测，是国际认可的标准化诊断方法。我国目前广泛应用该方法进行疫病流行病学调查和免疫抗体监测，评价疫苗免疫效力。     

副结核分枝杆菌MAP0862、MAP1345基因的串联表达及其在间接ELISA中的初步应用

为探索副结核特异性蛋白MAP0862与MAP1345在牛副结核病血清学诊断中的作用,将通过串联表达获得的融合蛋白MAP0862-1345纯化定量后包被酶标板,经过对反应条件的优化,初步建立了基于融合蛋白MAP0862-1345的间接ELISA诊断方法。使用建立的ELISA方法对牛副结核病阳性血清、牛结核病阳性血清、牛布病阳性血清、卡介苗免疫牛血清、健康牛血清检测的结果表明其具有较好的特异性;使用该方法与IDEXX副结核病抗体检测试剂盒共同对300份临床血清样本检测,总符合率为92.7%。     

黄芪多糖对PRRS疫苗免疫效果的研究

猪蓝耳病的发病率、死亡率较高,造成了较大的经济损失,引起社会的广泛关注。目前,我国已将高致病性猪蓝耳病的免疫工作列为重大动物疫病强制免疫。2007年下半年,各地动物疫病预防控制机构开始展开强制免疫工作[1]。应用ELISA检测猪蓝耳病(PRRS)血清抗体,特异性强,且敏感性高,已被许多国家应用[2-6]。英国已把ELISA方法作为PRRS监测的常规手段[7-10]。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.     

Mechanism of Action of Probiotic Function of Lactobacilli

乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制.     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …