共查询到20条相似文献,搜索用时 28 毫秒
1.
K. Jane Wardrop DVM MS Tina J. Owen Kenneth M. Meyers 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(4):253-257
The effect of an additive preservative solution on canine red blood cell posttransfusion viability (PTV) and on selected canine red blood cell biochemical parameters was studied. One unit (450 mL) of blood was collected from 6 clinically normal dogs into the anticoagulant citrate phosphate dextrose, centrifuged, and the plasma removed. The red blood cells were then suspended in 100 mL of a saline, adenine, dextrose, and mannitol solution and stored at 4°C. Aliquots were removed for study at 1, 10, 20, 30, 37, and 44 days. The 24-hour PTV of autologous red blood cells was determined using a sodium chromate (61 Cr) label. Red blood cell concentrations of 2,3-diphosphoglycerate (2,3-DPG), adenosine-5'-triphosphate (ATP), and pH were also determined. Canine red blood cell PTV, pH, ATP, and 2,3-DPG concentrations decreased during storage ( P < .05). The PTV decreased from 94% using day 1 red blood cells to 80% and 75% using day 37 and day 44 red blood cells, respectively ( P < .05). Although the mean PTV of the day 44 stored units equaled the Food and Drug Administration (FDA) minimum standard for human red blood cells, the PTV was substandard in 75% of the day 44 units. The FDA standard was exceeded in 83% of the day 37 units. It was concluded that 37-day-old canine red blood cells preserved with a saline, adenine, dextrose, and mannitol solution are of acceptable quality for transfusion. 相似文献
2.
Lori S. Waddell DVM DACVECC David E. Holt BVSC DACVS Dez Hughes BVSc MRCVS DACVECC Urs Giger PD DR. med. vet. DACVIM 《Journal of Veterinary Emergency and Critical Care》2001,11(1):23-26
Objective: To determine the effect of storage on ammonia concentration in canine packed red blood cell (pRBC) units.
Design: In vitro and in vivo study.
Setting: University Veterinary Teaching Hospital.
Interventions: Ammonia concentration was measured in 7 units of canine pRBC prepared in citrate-phosphate-dextrose (CPD) and Adsola on Days 1 and 35 of storage. Ammonia was measured in 4 additional units of canine pRBC on Days 0, 7, 14, 21, 28, and 35. Plasma ammonia was also determined in 5 anemic dogs receiving pRBC.
Measurements and Main Results: Ammonia concentration increased from 73 ± 15 mmol/L (mean ± SD) on Day 1 to 800 ± 275 mmpl/L on Day (p<0.001). When measured every 7 days in 4 units of canine pRBC, ammonia concentration increased from 23 ± 8 mmol/L on Day 0 to 179 ± 13 mmol/L (Day 7), 276 ± 56 mmol/L (Day 14). 383 ± 47 mmol/L (Day21), 466 ± 30 mmol/L (Day 28), and 562 ± 27 mmol/L (Day 35) (p<0.05 for all comparisons). In a preliminary study, plasma ammonia concentration measured in blood samples from 5 anemic dogs without primary liver disease immediately before and after transfusion with 5–10 ml/kg of stored pRBC remained in the normal reference range.
Conclusions: The ammonia concentration in stored canine pRBC increased markedly with time. In this preliminary study, ammonia concentrations in dogs without primary liver disease did not increase above the reference range after transfusion with pRBC. 相似文献
Design: In vitro and in vivo study.
Setting: University Veterinary Teaching Hospital.
Interventions: Ammonia concentration was measured in 7 units of canine pRBC prepared in citrate-phosphate-dextrose (CPD) and Adsol
Measurements and Main Results: Ammonia concentration increased from 73 ± 15 mmol/L (mean ± SD) on Day 1 to 800 ± 275 mmpl/L on Day (p<0.001). When measured every 7 days in 4 units of canine pRBC, ammonia concentration increased from 23 ± 8 mmol/L on Day 0 to 179 ± 13 mmol/L (Day 7), 276 ± 56 mmol/L (Day 14). 383 ± 47 mmol/L (Day21), 466 ± 30 mmol/L (Day 28), and 562 ± 27 mmol/L (Day 35) (p<0.05 for all comparisons). In a preliminary study, plasma ammonia concentration measured in blood samples from 5 anemic dogs without primary liver disease immediately before and after transfusion with 5–10 ml/kg of stored pRBC remained in the normal reference range.
Conclusions: The ammonia concentration in stored canine pRBC increased markedly with time. In this preliminary study, ammonia concentrations in dogs without primary liver disease did not increase above the reference range after transfusion with pRBC. 相似文献
3.
J. Stiles MS DVM M.R. Raffe DVM MS 《Journal of Veterinary Emergency and Critical Care》1991,1(2):50-53
Hemolysis produced by peristaltic pump infusion of canine whole blood was studied in fresh (3-hours-old) and stored (21-day-old) blood units. Blood was pumped at infusion rates of 50, 100, and 200 ml/hr and compared to gravity flow blood at the same rates. IVAC 530 and 560 (peristaltic) and the Animed Flowset 100 (rotaty) pumps were evaluated.
In the fresh blood group, the IVAC 530 did not produce significant hemolysis at any rate compared togravity infusion. Blood pumped through the IVAC 560 and the Animed Flowset 100 was significantly hemolyzed when compared to blood administered by gravity flow.
Free plasma hemoglobin was higher in stored blood than in fresh blood for all groups. The Animed Flowset 100 demonstrated significant hemolysis at 50 and 100 ml/hr flow rates compared to free-dripped blood, the IVAC 530, and the IVAC 560. All pumps hemolyzed blood more at 100 and 200 ml/hr than at 50 ml/hr.
When fresh blood is transfused, it can be pumped through an IVAC 530 at 50, 100, or 200 ml/hr without significant hemolysis. The IVAC 560 produces an intermediate level of hemolysis. The Animed Flowset 100 is not recommended for pumping whole blood. Blood that has been stored for 21 days appears to have a much higher level of hemolysis than fresh blood at all infusion rates. 相似文献
In the fresh blood group, the IVAC 530 did not produce significant hemolysis at any rate compared togravity infusion. Blood pumped through the IVAC 560 and the Animed Flowset 100 was significantly hemolyzed when compared to blood administered by gravity flow.
Free plasma hemoglobin was higher in stored blood than in fresh blood for all groups. The Animed Flowset 100 demonstrated significant hemolysis at 50 and 100 ml/hr flow rates compared to free-dripped blood, the IVAC 530, and the IVAC 560. All pumps hemolyzed blood more at 100 and 200 ml/hr than at 50 ml/hr.
When fresh blood is transfused, it can be pumped through an IVAC 530 at 50, 100, or 200 ml/hr without significant hemolysis. The IVAC 560 produces an intermediate level of hemolysis. The Animed Flowset 100 is not recommended for pumping whole blood. Blood that has been stored for 21 days appears to have a much higher level of hemolysis than fresh blood at all infusion rates. 相似文献
4.
5.
Raymond W. Sweeney Thomas J. Divers David T. Zamos Pamela A. Spencer 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1989,18(4):103-106
Sixty-three blood samples from 10 diarrheic calves were tested for glucose concentration by two methods. Plasma glucose concentration was measured by the conventional glucose-6-phosphate dehydrogenase method in the clinical laboratory, and the results compared to those obtained using a rapid reagent strip test for blood glucose concentration measurement. The rapid reagent strip test result could not be used to make an accurate prediction of the actual plasma glucose concentration as determined by the conventional method, due to the wide variability in actual plasma glucose concentrations corresponding to each rapid test result. 相似文献
6.
7.
为探讨表面抗原修饰后猪红细胞异源输血的可行性,采用α-半乳糖苷酶和单甲氧基聚乙二醇琥珀酰亚胺碳酸酯(mPEG-SC)对猪红细胞进行双修饰后,体外检测红细胞的渗透脆性,并进行配血试验和犬输血试验。观察输血前后犬的各项临床指标变化,并对其进行血常规和尿常规的连续检测分析。结果表明,修饰红细胞渗透脆性降低,体外配血未出现凝集反应。输血后亦未引起明显的临床异常反应,血常规及尿常规正常。说明猪红细胞体外双修饰后进行首次异源输血具有可行性,可作为动物通用血源用于临床。 相似文献
8.
The antioxidant status of the red blood cells of buffaloes (n = 20) suffering from post-parturient haemoglobinuria (PPH) was assessed by comparing their tocopherol (vitamin E) and reduced glutathione contents with those of red blood cells from apparently healthy buffaloes (n = 20). The red cell tocopherol content of the diseased buffaloes (1.76±0.11 g/ml) was significantly (p<0.01) lower than that of healthy buffaloes (2.45±0.14 g/ml). This may be the first report comparing the concentration of tocopherol in the red blood cells of buffaloes suffering from PPH and apparently healthy buffaloes.There was a drastic reduction in the reduced glutathione content in the red cells of haemoglobinuric buffaloes (23.74±2.86 mg%) compared to the healthy control buffaloes (73.71±3.87 mg%). The diseased buffaloes also exhibited severe hypophosphataemia. These findings suggest that an impaired or insufficient antioxidant potential of the red blood cells in this disease in buffaloes is associated with the phosphorus deficiency. 相似文献
9.
《中国兽医杂志》2014,(5)
为了研究脂肪干细胞(Adipose-derived stem cells,ASCs)体外培养方法及其生物学特性,将ASCs应用于小动物临床治疗,本试验用犬进行了脂肪干细胞的分离、培养和鉴定。犬脂肪干细胞(Canine adipose-derived stem cells,cASCs)体外培养生长良好,呈细长梭形。第3代(P3)细胞诱导2周后,成脂诱导组经油红O染色可见细胞内脂滴积累;成骨诱导组经碱性磷酸酶(Alkalinephosphatase,AKP)及VonKossa染色,AKP、钙结节均呈阳性表达。第3代至第5代(P3P5)细胞流式细胞检测结果显示,所分离细胞稳定高表达间充质干细胞(MesenchymalStem Cells,MSCs)标志CD29、CD44、CD90(>90%),不表达造血细胞标志CD45(<2%)。培养结果显示,从犬镰状韧带和皮下脂肪均可分离出cASCs。cASCs取材方便,易于体外培养,多次传代及冻存对cASCs的生物学性质无明显影响。eASCs有望成为国内动物医学临床研究和治疗的重要干细胞来源。 相似文献
10.
11.
应用红细胞促淋巴细胞转化和促NK细胞活性试验,对不同生理发育时期日本大耳白兔红细胞调控淋巴细胞和NK细胞活性进行研究。结果表明:青年组日本大耳白兔红细胞调控淋巴细胞能力明显高于幼年兔和老年兔(P〈0.01);老年组红细胞调控NK活性能力明显高于青年组和幼年组(P〈0.01),青年组高于幼年组(P〈0.01)。日本大耳白兔红细胞调控淋巴细胞能力在青年时期达到了高峰.随后逐渐降低:而红细胞调控NK活性随年龄增长而显著增强,这提示老年兔的免疫活性细胞较多地处于预激活状态,并对刺激显示良好的免疫应答能力。 相似文献
12.
用常规法测定了青海省湟中县35头成年黄牛的血乳酸,全血钾、血清钠、血糖、血清无机磷浓度。测定结果分别为2.33±1.48mmol/L,12.73±6.17mmol/L,4.06±1.04mmol/L,129.32±14.18mmol/L,2.64±0.58mmol/L。 相似文献
13.
Evaluation of Citrate-Phosphate- Dextrose-Adenine as a Storage Medium for Packed Canine Erythrocytes 总被引:1,自引:0,他引:1
G. Sylvester Price DVM P. Jane Armstrong DVM MS Deborah A. McLeod DVM Cynthia A. Babineau Michael R. Metcalf DVM MS Louis C. Sellett MS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1988,2(3):126-132
Packed canine red blood cells (RBCs) stored in the anticoagulant-preservative solution citrate-phosphate-dextrose-adenine (CPDA-1) were studied at 1, 10, 20, 30, and 40 days. The extracellular concentrations of potassium and sodium, erythrocyte mean corpuscular volume, and osmotic fragility increased during storage (P less than 0.05). There was a decrease in the pH, plasma concentration of glucose, and erythrocyte concentrations of 2,3-diphosphoglycerate (2,3-DPG) and adenosine-5'-triphosphate (P less than 0.05). Erythrocyte 2,3-DPG concentration decreased by 54% within the first 24 hours of storage (P less than 0.001). Posttransfusion viability (PTV) decreased from 90% on day 1 to 46% on day 40 (P less than 0.05). The PTV of the RBCs stored for 10 and 20 days complied with the Food and Drug Administration (FDA) standard. Although there are marked biochemical and hematologic changes in stored packed red blood cells (pRBCs), 20-day-old units may be expected to be of acceptable quality. The sharp decrease in 2,3-DPG concentration suggests a reduction in oxygen carrying capacity in erythrocytes stored as pRBCs. Hyperkalemia occurs during storage of pRBCs and does not appear to be associated with high intraerythrocytic potassium concentrations. 相似文献
14.
15.
Richard J. Joseph DVM Kay Allyson BS Thomas K. Graves Mark J. Rondeau PhD Mark E. Peterson DVM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1987,1(4):170-174
We evaluated three reflectance meters (Accu-Chek II, Glucometer II, and Glucoscan 2000) and two reagent strips (Chemstrip bG and Glucostix) for accuracy and precision in determining blood glucose concentrations in the dog. To evaluate accuracy, we compared results of blood glucose determinations performed on 95 samples using the various strips and meters vs. the glucose concentrations obtained using the glucose-oxidase method on a Beckman Glucose Analyzer. Accuracy was evaluated statistically using least squares regression analysis. To evaluate precision, samples in various ranges of blood glucose concentration were tested repeatedly (20 times within a 1-hour period) on the same reflectance meter. Coefficient of variation (CV) was determined to evaluate reproducibility of results. Overall, there were significant correlations (P less than 0.001) between the laboratory glucose values and the blood glucose concentrations obtained with Chemstrip bG (r = 0.976), Glucostix (r = 0.904), Accu-Chek II (r = 0.986), Glucometer II (r = 0.911) and Glucoscan 2000 (r = 0.944). In the precision study, all three meters had excellent CVs in the normal range (3.6% to 4.9%). However, Accu-Chek II was found to be more precise in the hypoglycemic and hyperglycemic ranges (3.6% and 2.6%, respectively) than either Glucometer II (8.8% and 5.4%) or Glucoscan 2000 (7.8% and 8.2%). The results of this study indicate that all of the meters and reagent strips tested are highly accurate in determining blood glucose concentrations in the dog. However, both in terms of accuracy and reproducibility of results, Accu-Chek II and Chemstrip bG, gave the highest correlation coefficients and, as such, are probably of the greatest clinical value. 相似文献
16.
We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by western blot using horse-specific antibodies. Statistical analyses included unpaired Student t test and chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters (HTs) and the remaining 12% as low lactate transporters (LTs). The opposite was found for the European Warmbloods, where most animals (77%) were classified as LTs and the remaining animals (23%) were classified as HTs. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs. 相似文献
17.
Oberbauer AM Grossman DI Eggleston ML Irion DN Schaffer AL Pedersen NC Belanger JM 《Veterinary research communications》2003,27(1):27-38
Participation and compliance are critical to the success of any large-scale study of canine disease using DNA markers. Most canine genetic studies rely upon DNA extracted from peripheral blood samples. We assessed the utility of buccal swab epithelial cells and toe nails as a source of DNA for use in genomic screening studies. Using eight multiplexed canine microsatellite markers, amplified DNA obtained from peripheral blood, and from freshly extracted buccal epithelial cells, and buccal swab DNA extracted and stored at –20°C for 27 months or extracted from toe nails were compared for three dogs. The accuracy of the genotyping at each locus was identical for each preparation. Buccal swab DNA samples were readily and uniformly amplified and could be stored for years without loss of integrity. Each buccal swab provided sufficient DNA for more than 200 individual PCR reactions. Toe nails provided ample DNA for thousands of PCR reactions and had the added advantage of ease of storage of the original tissues. These studies demonstrate the potential utility of DNA derived from buccal swabs or nails in large-scale genomic scanning and marker linkage studies. 相似文献
18.
Much of our knowledge regarding vertebrate blood and blood cells is based on mammalian references. The critical study of nonmammalian vertebrate blood is relatively new, and comparatively few investigations have been published that focus on these animals' ontogeny and structure-function relationships of blood cells. Nonmammalian vertebrates comprise birds, reptiles, amphibians, and fishes, all of which have a wide range of forms and adaptations. For the clinician, a lack of basic knowledge with these species makes the evaluation of a hemogram more challenging than in mammals. This is a concise review of our current knowledge of comparative morphology that describes routine staining procedures and the development and function of blood cells in nonmammalian vertebrates. 相似文献
19.
日粮锌和含硫氨基酸水平对鸡红细胞脆性的影响 总被引:2,自引:0,他引:2
考察了鸡红细胞脆性在不同日粮锌和含硫氨基酸水平下的变化。结果表明,日粮加锌、加含硫氨基酸对鸡红细胞脆性都有极显著的影响。 相似文献
20.
试验比较了MA-104、Marc-145、Vero 3种猴肾细胞增殖犬瘟热病毒的敏感性,按常规方法将犬瘟热病毒(CDV-XN112株)分别接种于MA-104、Marc-145、Vero 3种细胞,通过观察细胞病变,确定收毒时间,比较病毒滴度。结果表明,犬瘟热病毒可在MA-104、Marc-145、Vero 3种细胞内增殖,并出现典型细胞病变,效价均达到104.5TCID50/0.1mL以上,而Vero细胞增殖犬瘟热病毒更为经济高效,是增殖该病毒的理想细胞。 相似文献