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本文首次成功地建立了检测副鸡嗜血杆菌(hpg)抗原的间接法Dot-ELISA。Hpg抗原的最低检出量为3.91×10 ̄5CFU/ml。Hpg人工感染样本的抗原阳性检出率为:口咽试子95.5%;鼻窦及眶下分泌物拭子90.9%。经统计学分析,二者在5%显著水平无显著差异。人工感染后4天及8天口咽拭子的抗原阳性检出率分别为100%和95.5%。经统计学分析,二者在5%显著水平无显著差异。Hpg自然感染样本的抗原检出率为:口咽拭子81.7%,鼻分泌物拭子77.8%,眶下窦分泌物拭子76.9%。间接法Dot-ELISA检测抗原经济、快速、操作简便,有较好的敏感性、特异性和重复性,能有效检出Hpg人工及自然感染病鸡的带菌菌况,适合于大规模抗原样本的检测,可以用作疫病早期诊断和进行流行病学调查。 相似文献
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间接法Dot—ELISA检测副鸡嗜血杆菌的研究 总被引:1,自引:0,他引:1
本文首次成功地建立了检测副鸡嗜血杆菌抗原的间接法Dot-ELISA,Hpg抗原的最低检出量为3.91×10^5CFU/ml。Hpg人工感染样本的抗原阳性检出率为:口咽试子95.5%;鼻窦及眶下分泌物拭子90.9%。经统计分析,二者在5%显著水平无显著水平无显著差异。人工感染后4天及8天口咽拭子的抗原阳性检出率分别为100%和95.5%。经统计学分析,二者在5%显著水平无显著差异,Hpg自然感染样本 相似文献
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本研究采用阻断ELISA(B-ELISA)和血凝抑制试验(HI)方法,检测了鸡体分别人工感染副鸡嗜血杆菌Hp8株(A型)和H668株(C型)后0-9周的血清抗体消长情况,并绘制了示意抗体曲线。结果表明,Hp8人工感染后,从第2周到第9周,B-ELISA阳性检出率一直保持100%,其中抗体效价在感染后第4周达到效价高峰,平均约为15.2。而H688人工感染鸡则在感染后第7周检出率最高,此后急剧下降。AI方法对A型抗体的阳性检出率亦在在第4-5周达到高峰,而对C型抗体的检出率一直较低。结果进一步显示了B-ELISA良好的特异性和敏感性,为本方法及其试剂盒产品的进一步应用提供了参考。 相似文献
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副鸡嗜血杆菌种特异性的核酸探针陈小玲J.K,Miflin张培君P.J.Bleckall(北京市农业科学院畜牧兽医研究所北京,100081)鸡传染性鼻炎(IC)是由副鸡嗜血杆菌(Hpg)引起的呼吸道传染病。其危害主要在于影响鸡的生长和使鸡产蛋率下降。诊... 相似文献
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B型副鸡嗜血杆菌的分离鉴定 总被引:9,自引:2,他引:9
从辽宁某公司鸡场疑似鸡传染性鼻炎的病鸡眶下窦分离到一株副鸡嗜血杆菌,用Page程序和Kume程序对其进行血清型鉴定,确认为B型副鸡嗜血杆菌,这是首次在我国分离到B型副鸡嗜血杆菌。 相似文献
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二株副鸡嗜血杆菌分离株的生物学特性鉴定 总被引:2,自引:0,他引:2
本试验对从广西分离的4株疑似副鸡嗜血杆菌(H.pg)(GX-95,GX-97,GX-98-1,GX-98-2)进行生物学特性研究。根据生物学特性,GX-97和GX-98-1被定为H.pg,GX-95和GX-98-2的生化反应特性有明显差异,用标准H.pg血清未能定型。交叉血凝抑制(HI)试验显示,各菌株之间的交叉HI效价达160,但各菌株与其本身的抗血清HI效价达1280,显示出很强的菌株特异性。抗菌素敏感试验也显示GX-97、GX-98-1和参考菌株的药敏谱更加相似。 相似文献
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Dot—ELISA检测副鸡嗜血杆菌血清型特异性抗原的研究 总被引:4,自引:0,他引:4
本研究在副鸡嗜血杆菌型特异性单克隆抗体的基础上建立了检测该菌 A、 C 型特异性抗原的 Dot E L I S A 方法,结果表明本方法在检测副鸡嗜血杆菌型特异性抗原时未呈现与其它 8 种常见病原体的交叉反应,新鲜肉汤的最低检出量为 A 型 13×106 C F U/m l和 C 型 8×106 C F U/m l,抗原的最低检出量均为 25μg/m l,对 20 份抗原、肉汤和卵黄样品的检测结果与其已知结果相符。这就证明本方法具有较好的敏感性和特异性,是一种能直接检测分离株血清型的良好方法。 相似文献
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Two isolates of haemophilic bacteria originally isolated in the 1980s from chickens were re-examined. The addition of a 10% sterile filtrate from an overnight culture of Staphylococcus epidermidis allowed growth of both isolates in solid and liquid media that were otherwise not capable of supporting the growth of these isolates. Using the modified media, genotypic and serotypic studies were performed, which confirmed both isolates to be Avibacterium paragallinarum, with one isolate being serovar A and the other serovar C. The unusual growth requirements of these two isolates reinforces the need for careful interpretation by diagnostic laboratories examining chickens showing signs of upper respiratory tract disease. 相似文献
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OBJECTIVE: To characterise 18 isolates of Haemophilus paragallinarum isolated from chickens in Indonesia. PROCEDURE: The isolates were identified to species level by traditional phenotypic methods. Six of the isolates were also identified by a species-specific polymerase chain reaction. Fourteen of the isolates were examined for resistance to a panel of seven antimicrobial agents using a disc diffusion method. All 18 isolates were serotyped according to the Page scheme using reference antisera in a haemagglutination inhibition test. RESULTS: Four of the 18 isolates were obtained from indigenous (kampung) chickens, with the remainder being from typical intensive poultry production systems. The 18 isolates were obtained from 11 outbreaks that showed the typical clinical signs of infectious coryza and 11 of the isolates were obtained from chickens that had been vaccinated with infectious coryza vaccines. All 18 isolates were confirmed as H paragallinarum by biochemical testing and six isolates were also identified as H paragallinarum by the polymerase chain reaction test. Eleven isolates were resistant to erythromycin and streptomycin, 10 to neomycin, eight to oxytetracycline, five isolates to doxycycline, three to sulphamethoxazoltrimethoprim but only one to ampicillin. Seven isolates were Page serovar A, four were Page serovar B and seven were Page serovar C. CONCLUSION: The presence of all three Page serovars (A, B and C) has been confirmed for the first time in Indonesian chickens. As the majority of the infectious coryza vaccines in use in Indonesia contain only serovar A and C, the presence of serovar B in chickens indicates that the protection by these bivalent vaccines would be reduced. The use of trivalent infectious coryza vaccines that contain serovars A, B and C is recommended for use in Indonesia. 相似文献
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副鸡嗜血杆菌的分离鉴定及我国鸡传染性鼻炎的流行状况分析 总被引:8,自引:0,他引:8
从不同地区免疫失败鸡场的鸡传染性鼻炎疑似鸡体内共分离到19株细菌,经回归试验、PCR等方法鉴定为副鸡嗜血杆菌。又将分离株用血清平板凝集试验、血凝抑制试验和型特异性单抗进行了血清型的鉴定,结果表明分离菌有15株为A型,4株为B型,说明我国鸡传染性鼻炎的流行已经出现了新的特点,在疾病预防和控制中应加以注意。另外大多数鸡场的免疫失败是由于免疫效果不良所致,但也有相当一部分鸡场的免疫失败是由于B血清型的出现引起的。 相似文献