共查询到20条相似文献，搜索用时 35 毫秒
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J Bluford D Gauthier M Colasanto M Rhodes W Vogelbein A Haines 《Journal of fish diseases》2017,40(4):597-600
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Outbreak of luminescent disease was reported from Litopenaeus vannamei shrimp farms in Zhangpu County, Southern China during May–July 2011. The clinical signs included fluorescent, less food consumption and high mortality. Bacteria were isolated from the infected shrimps. The pathogen, a luminescent bacterium named VH1 was identified as Vibrio campbellii based on MLSA analysis (16S rDNA, rpoD and toxR). The haemolysin (hly) gene specific in V. campbellii was detected in strain VH1. Pathogenicity test using immersion infection confirmed that strain VH1 was virulent to L. vannamei postlarvae and juveniles, and the LC50 value was 1.55 × 106 CFU mL?1 and 1.7 × 106 CFU mL?1 respectively. 相似文献
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Gen Hua Yue 《Fish and Fisheries》2014,15(3):376-396
Aquaculture is the fastest growing sector in agriculture. Substantial genetic gains have been achieved in a few cultured species using conventional selective breeding approaches. However, the majority of fish and shellfish species remain in their wild state. Due to the recognition of the enormous potential of marker‐assisted selection (MAS) to speed up genetic gain through early selection, aquaculture scientists have constructed linkage maps in over 40 species and mapped quantitative trait loci (QTL) for important traits in over 20 species since the 1990s. Although MAS and genomic selection (GS) have not been widely used in aquaculture, their application in breeding programmes is expected to be a fertile area of research. In this paper, I summarized the recent advances of linkage and QTL mapping, as well as MAS in aquaculture species. I also discussed the potentials of genome‐wide association studies (GWAS) and GS in aquaculture species. 相似文献
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Bianca H. Ventura Fernandes Caroline Caetano da Silva Debora Bissegato Michael L. Kent Luciani R. Carvalho 《Journal of fish diseases》2022,45(1):35-39
Along with the growing number of laboratories that work with zebrafish (Danio rerio), it is necessary to have animals with good sanitary quality. Specific pathogens can interfere with the experimental results and in the life quality of the animals. Pseudoloma neurophilia is a parasite with high potential for interference in behavioural, morphology, toxicological and genetic research, and is very common in zebrafish facilities. With that, we implemented a protocol for the pathogen elimination in a genetically modified lineage (prop 1) using eggs from specific pathogen-free (SPF) wild-type fish (AB line) for in vitro fertilization, along with water recirculation equipment disinfection, appropriate PCR screening and back crossing protocols. This resulted in SPF prop 1 heterozygotes, which allowed us to move forward with subsequent crossings to develop homozygote prop 1 mutants for our research. Hence, this demonstrates a useful strategy for an individual research laboratory to rederive a specific mutant free line that is not available from other SPF laboratories. 相似文献
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Fengrong Zheng Hongzhan Liu Yongqiang Zhang Zongjun Xu Bo Wang 《Aquaculture Research》2017,48(2):521-530
Scophthalmas maximus is a precious and main aquacultural species in northern China with an annual output value of more than 1.5 billion yuan. However, with the expansion of breeding range, increased stocking density and variety degradation, a variety of bacterial, parasitic and viral diseases have often occurred and worsened. Among them, bacterial disease is the most serious in farmed S. maximus. Since 2010, an epidemic bacterial disease with primary symptoms of lighter colour of lateral and caudal fins, spotty bleeding, fin base bleeding, abdominal swelling and redness and lesions of internal organs has put broken in S. maximus cultured in Weihai, Longkou and Haiyang, Shandong Province, China. The disease occurred mostly at the seedling and early development stages and propagated very fast, resulting in high mortality. These S. maximus died with obvious surface ulceration in only 3–4 days, causing great economic losses to the aquaculture industry. Beginning in 2010, we conducted epidemiological investigation in farmed S. maximus and isolated two different pathogens from the body of the diseased S. maximus from different farms. In this paper, we observed their morphology, conducted phylogenetic analysis based on their physiological and conventional biochemical characteristics and 16S rRNA gene sequences, preliminarily analysed their pathogenesis and investigated the histopathological changes of the diseased S. maximus. We believed that Vibrio anguillarum and V. parahaemolyticus were the pathogenic bacteria for the epidemic disease in S. maximus. 相似文献
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Jie Li Siqing Chen Changlin Liu Guiyang Li Zhaolan Mo Jie Huang 《Aquaculture Research》2018,49(5):2078-2080
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《Reviews in Aquaculture》2018,10(3):585-610
Vibrio (Listonella ) anguillarum is a marine bacteria that is pathogenic to a number of aquatic organisms including several species which are important to the aquaculture industry. Organisms that are infected by V. anguillarum are diagnosed with vibriosis which can be lethal in a matter of days. Vibriosis can be particularly devastating to aquaculture businesses and measures of prevention or treatment are generally quite expensive. Efforts to understand and control V. anguillarum virulence have been of high‐priority among international aquatic research studies. The knowledge that has accumulated as a result of this collective research effort is reviewed in this article. 相似文献
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Ramesh Chandra Malick Asit Kumar Bera Hemanta Chowdhury Manojit Bhattacharya Tanuja Abdulla Himanshu Sekhar Swain Raju Baitha Vikas Kumar Basanta Kumar Das 《Aquaculture Research》2020,51(6):2410-2420
The disease outbreaks in aquaculture system of wetlands are the major cause of fish mortality. Among various bacterial septicaemic diseases, fish mortality caused by Acinetobacter spp. is recently reported in different fish species. Fish disease outbreak was investigated in a wetland of West Bengal, India to identify the aetiological factors involved. The moribund fish were examined and subjected to bacterial isolation. Two bacterial causative agents were identified as Acinetobacter junii and Acinetobacter pittii by biochemical characterization and 16S rRNA gene amplification. Both the isolates were oxidase‐negative, nitrate‐negative, catalase‐positive and indole‐negative. The molecular identification using 16S rRNA gene sequencing and phylogenetic tree analysis further confirmed the two Acinetobacter spp. with 97%–99% similarity. The antibiotic resistance patterns of these two bacteria revealed that both of them were resistant to β‐lactam, cefalexin, cephalothin, amoxyclav, cefuroxime, cefadroxil, clindamycin, vancomycin and penicillin. In addition, A. pittii was also resistant to other antibiotics of cephams group such as ceftazidime and cefotaxime. In the challenge experiment, both A. junii and A. pittii were found to be pathogenic with LD50 of 1.24 × 105 and 1.88 × 107 cfu/fish respectively. Histopathological examination of gill, liver and kidney revealed prominent changes supporting bacterial septicaemia. The investigation reports for the first time on concurrent infection by A. junii and multidrug‐resistant (MDR)‐A. pittii as emerging fish pathogens to cause severe mortality in Labeo catla and Hypophthalmichthys molitrix in a freshwater wetland. 相似文献
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Ruben Avendaño-Herrera Soledad Núñez Juan L. Barja Alicia E. Toranzo 《Aquaculture International》2008,16(1):1-11
The in vitro susceptibility of 63 isolates of Tenacibaculum maritimum from four fish farms to eight chemotherapeutic agents used for the treatment of bacterial diseases in fish were assessed.
The results indicated that all strains were resistant to oxolinic acid and susceptible to amoxicillin, nitrofurantoin, florfenicol,
oxytetracycline and trimethoprim-sulphamethoxazole. However, some isolates presented resistance to enrofloxacin and flumequine,
ranging from 10 to 30%, and from 25 to 60%, respectively, depending on the farm sampled. These data were used in an attempt
to predict whether the resistance to enrofloxacin was static or evolved during the time of sampling from 2003 to 2004. A relationship
between the use of enrofloxacin and levels of resistance was detected in the studied farm, increasing significantly from no
resistant isolates in 2003 to 44.8% resistant strains in 2004, the year in which this drug was commonly employed. This result
was accompanied by a marked decline of about 29.2% of the inhibition zone sizes for the T. maritimum strains in comparison to the initial values (average 21.5 mm). Minimum inhibitory concentration (MIC) of enrofloxacin for
100 T. maritimum strains was determined by the microdilution method. Twenty isolates were resistant to enrofloxacin (> 256 μg ml−1), while the remaining strains showed a bimodal distribution, which ranged from 0.5 to 32 μg ml−1. Our interpretation of the enrofloxacin MIC data suggests that the breakpoint for T. maritimum should be 4 μg ml−1. However, similar studies in other laboratories are necessary to validate this breakpoint value. 相似文献
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Bacterial kidney disease (BKD) is considered an important cause of loss in salmon aquaculture in Atlantic Canada. Causative agent of BKD is the Gram‐positive bacteria Renibacterium salmoninarum. Infected salmon are often asymptomatic (subclinical infection), and the disease is considered chronic. One of the challenges in quantifying information from farm production and health records is the application of a standardized case definition. Case definitions for farm‐level and cage‐level clinical and subclinical BKD were developed using retrospective longitudinal data from aquaculture practices in New Brunswick, Canada, combining (i) industry records of weekly production data including mortalities, (ii) field observations for BKD using reports of veterinarians and/or fish health technicians, (iii) diagnostic submissions and test results and (iv) treatments used to control BKD. Case definitions were evaluated using veterinarians’ expert judgements as reference standard. Eighty‐nine and 66% of sites and fish groups, respectively, were associated with BKD at least once. For BKD present (subclinical or clinical), sensitivity and specificity of the case definition were 75–100% varying between event, fish group, site cycle and level (site pen). For clinical BKD, sensitivities were 29–64% and specificities 91–100%. Industry data can be used to develop sensitive case definitions. 相似文献
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Fang Li Di Wu Hao‐Ran Gu Min Yin Hai‐Long Ge Xiao‐Hong Liu Jing Huang Yao‐Guang Zhang Zhi‐Jian Wang 《Aquaculture Research》2019,50(5):1515-1526
In August 2017, a serious disease causing high mortality occurred in a Myxocyprinus asiaticus aquaculture farm. According to necropsy findings, bacteriology experiments and phylogenetic analysis based on 16S rRNA, cpn60, gyrB and rpoB genes and concatenated alignment sequences (cpn60, gyrB and rpoB genes), two isolates, that is, BBAh1 and BBAv1, were identified as Aeromonas hydrophila and Aeromonas veronii respectively. Artificial infection experiments were carried out, showing that the BBAh1 and BBAv1 strains can cause similar symptoms and have LD50 values of 1.93×105 and 8.77×105 cfu/g respectively. In addition, some virulent genes coding for AerA, Alt, Ast, AscV, AexT, AspA, HlyA, OmpA, Lip and FlaA were detected in the two strains. Furthermore, BBAh1 and BBAv1 showed the same sensitivities to 28 antibiotics, some of which, such as cefotaxime, aztreonam, ceftriaxone and tetracycline, may be used to control the disease. However, the strains were also resistant to many antibiotics. These results provide a scientific reference for the diagnosis, prevention and treatment of motile Aeromonas septicaemia caused by A. hydrophila or A. veronii in cultured Chinese sucker. 相似文献
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Brett D. Glencross Johanna Baily Marc H.G. Berntssen Ronald Hardy Simon MacKenzie Douglas R. Tocher 《Reviews in Aquaculture》2020,12(2):703-758
A wide range of raw materials are now used routinely in aquaculture feeds throughout the world, primarily to supply protein and energy in the form of lipid from edible oils. Protein meals and oils used can generally be divided into those of plant or animal origin and many have considerable potential to supply the required dietary nutrients required by aquaculture species. However, the use of any raw material introduces a suite of risks that need to be considered to enable the production of safe, sustainable and functional feeds to underpin this sector. A lack of understanding of some of those risks can result in failure of dietary specifications being met and/or negative nutritional elements being introduced (e.g. antinutritional factors). Importantly, it is this feed that when fed to food‐producing animals is such an important element of food safety, and as such any undesirable aspects relating to feed production can also have a negative impact on the rest of the food chain. However, there is some disparity internationally among raw materials that are used and the perceptions surrounding the risk of their use. It is the scientific assessment of these risks that is the basis of this review. 相似文献
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Due to increasing resistance to chemical therapeutants, the use of ‘cleaner fish’ (primarily wrasse, Labridae, species) has become popular in European salmon farming for biocontrol of the salmon louse, Lepeophtheirus salmonis (Krøyer). While being efficient de‐licers, cleaner fish mortality levels in salmon cages are commonly high, and systemic bacterial infections constitute a major problem. Atypical furunculosis, caused by Aeromonas salmonicida A‐layer types V and VI, is among the most common diagnoses reached in clinical investigations. A previously described real‐time PCR (qPCR), targeting the A. salmonicida A‐layer gene (vapA), was modified and validated for specific and sensitive detection of all presently recognized A‐layer types of this bacterium. Before stocking and during episodes of increased mortality in salmon cages, cleaner fish (primarily wild‐caught wrasse) were sampled and screened for A. salmonicida by qPCR and culture. Culture indicated that systemic bacterial infections are mainly contracted after salmon farm stocking, and qPCR revealed A. salmonicida prevalences of approximately 4% and 68% in pre‐ and post‐stocked cleaner fish, respectively. This underpins A. salmonicida's relevance as a contributing factor to cleaner fish mortality and emphasizes the need for implementation of preventive measures (e.g. vaccination) if current levels of cleaner fish use are to be continued or expanded. 相似文献
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This study describes a novel multilocus variable number tandem repeat analysis (MLVA) based on six variable number of tandem repeat (VNTR) loci for genotyping of 37 Edwardsiella piscicida (previously Edwardsiella tarda) isolates from multiple sources. The number of alleles identified for each of the six VNTR loci ranged from 3 to 5 with VNTR loci 1 (DI = 0.632) and 3 (DI = 0.644), displaying the highest degrees of polymorphism. MLVA typing of the 37 E. piscicida isolates resulted in the identification of five major clusters consistent with their geographical origins, and were designated as MLVA types I, II, III, IV and V. Types III and V were resolved further into subtypes largely consistent with outbreak source. An MLVA profile comprising a string of integers representing the number of tandem repeats for each allele provided a unique identification for each MLVA type and/or strain. The MLVA protocol described in the current study is robust, relatively simple, has a higher power of resolution than multilocus sequence analysis (MLSA) and is capable of discriminating closely related isolates. 相似文献
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S Dutta S Biswas K Mukherjee U Chakrabarty A Mallik N Mandal 《Journal of fish diseases》2014,37(5):471-480
White spot disease (WSD) caused by white spot syndrome virus (WSSV) creates severe epizootics in shrimp aquaculture industry worldwide. Despite several efforts, no such permanent remedy was yet developed. Selective breeding using DNA markers would be a cost‐effective strategy for long‐term solution of this problem. In the present investigation, out of 30 random primers, only one primer produced a statistically significant (P < 0.01) randomly amplified polymorphic DNA (RAPD) marker of 502 bp, which provided a good discrimination between disease resistant and disease susceptible populations of Penaeus monodon from three geographical locations along the East coast of India. Because RAPD markers are dominant, a sequence characterized amplified region (SCAR) marker was developed by cloning and sequencing of 502 bp RAPD fragment, which generates a single 457 bp DNA fragment after PCR amplification only in the disease resistant shrimps. Challenge experiment was also conducted to validate this 457 bp SCAR marker, and the results suggested that the WSSV loads were 2.25 × 103 fold higher in disease susceptible than that in disease resistant shrimps using real‐time PCR. Therefore, this 457 bp DNA SCAR marker will be very valuable towards the development of disease‐free shrimp aquaculture industry. 相似文献
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Vibrio alginolyticus as an important pathogen in aquaculture can encounter the oxidative stress produced by the immune system during infection. Previous studies showed that sRNAs have important functions in response to oxidative stress in bacteria; however, less of sRNAs related to oxidative stress response were identified in V. alginolyticus. In this study, a total of 749 novel sRNAs were identified by RNA sequencing; among them, 128 sRNAs were up‐ or downregulated in response to oxidative stress. In addition, 1,870 genes exhibited variation on mRNA levels in oxidative stress response. By analysing the target genes of the sRNAs, we concluded that these sRNAs could regulate expressions of genes responsible for iron transport, catalase, GSH‐dependent defence system, electron transferred and stress response. Moreover, the functions of the sRNAs are also seemed related to the pathogenicity in V. alginolyticus. Based on the results, we constructed the oxidative stress model in V. alginolyticus. This study provides us the first outlook of sRNAs function in oxidative stress response in V. alginolyticus. Furthermore, this study can help us to prevent and control this important opportunistic pathogen in aquaculture. 相似文献
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