The enhanced mineralization of simazine and bentazon in soil after plant uptake

[triazine-ring-¹⁴C] simazine and [benzene-ring-¹⁴C] bentazon were added to the epipedon of a Luvisol from loess with and without maize shoots or roots (2 g/100 g soil) and mineralization proceeded in accordance with the standardized BBA degradation method at 50% of the maximum water holding capacity of the soil and at 22°C. The same degradation study was conducted using maize shoots (simazine) and maize roots (bentazon) which had taken up either ¹⁴C-simazine or ¹⁴C-bentazon from soil application. After 93 days of incubation 6.6% (simazine) or 7.2% (bentazon) of this plant incorporated ¹⁴C was mineralized to ¹⁴CO₂. This was 4–10 times greater than the mineralization of active ingredients applied to the soil and 4–6 times higher when compared to variants which in addition received maize shoots (simazine) or roots (bentazon) as an additional energy source for microbial development. Apparently as a consequence of the more intensified degradation processes, the bound residue fractions were higher by a factor of 2 when the residual radiocarbon reached the soil already incorporated into plant material.     

不同施肥处理下旱地红壤中阿特拉津的环境归趋及其调控研究

本研究采集长期施用化肥或有机肥(猪粪)的旱地红壤,设置单一或复合添加木质素和牡蛎壳粉的不同组合处理,进行微宇宙试验。采用同位素示踪、定量PCR、高通量测序等方法,研究施肥及土壤改良措施对红壤中阿特拉津矿化特征及降解微生物的调控作用。结果表明,在14周培养期间,旱地红壤对阿特拉津的矿化率低于0.33%。牡蛎壳粉有效提高红壤p H、改变土壤细菌群落,将施化肥、有机肥土壤中矿化率大幅提高至43.3%和9.51%,同时导致atz C和trz N等阿特拉津降解功能基因的富集。木质素则显著促进阿特拉津残留态形成,使阿特拉津与土壤有机质的结合达对照组的6.1倍,但对污染物的矿化没有明显作用。本研究结果有助于阐明旱地红壤中阿特拉津的环境归趋,并为发展适用于红壤的污染物控制技术提供科学依据。     

不同含水量及冻结温度对黑土冻融循环过程有机碳矿化的影响

[目的] 冻融过程土壤呼吸在年土壤呼吸总量中占有重要比例,研究探讨土壤冻融过程中含水量、冻结温度和冻融循环次数对土壤碳矿化动态的影响。[方法] 以黑龙江省嫩江县鹤山农场九三水土实验站黑土为研究对象,开展室内冻融程度模拟试验,进行7次冻融循环,设置100%田间持水量(100%WHC)、60%田间持水量(60%WHC)和30%田间持水量(30%WHC)3种土壤含水量;10 ℃恒温处理(对照)、-5 ℃冻结处理(轻度冻结)和-15 ℃冻结处理(重度冻结)3种环境温度。[结果] 冻融循环次数、含水量和冻结温度对CO₂排放量有显著影响,影响度分别为-0.63,0.21,0.14。解冻过程显著增加土壤碳矿化量;轻度冻结时,前3次冻融循环60%WHC土壤碳矿化量比100%WHC和30%WHC分别提高33.0%,35.2%,后4次冻融循环差异不明显;重度冻结时,前2次冻融循环100%WHC土壤碳矿化量,比60%WHC和30%WHC土壤分别提高25.2%,68.0%,后5次冻融循环差异不明显。[结论] 冻融循环次数对土壤CO₂排放量影响最大,含水量次之,冻结温度最小。冻融作用增加低含水量土壤的CO₂累积排放量;降低高含水量土壤的CO₂累积排放量;而对中等含水量土壤,轻度冻结增加CO₂累积排放量,重度冻结降低CO₂累积排放量。一级动力学方程对冻融土壤CO₂排放量的拟合效果较好(R²>0.997),含水量和冻结温度对有机碳矿化潜力C₀值有显著影响。     

Metabolism and persistence of atrazine in several field soils with different atrazine application histories

To assess the potential occurrence of accelerated herbicide degradation in soils, the mineralization and persistence of (14)C-labeled and nonlabeled atrazine was evaluated over 3 months in two soils from Belgium (BS, atrazine-treated 1973-2008; BC, nontreated) and two soils from Germany (CK, atrazine-treated 1986-1989; CM, nontreated). Prior to the experiment, accelerated solvent extraction of bulk field soils revealed atrazine (8.3 and 15.2 μg kg(-1)) in BS and CK soils and a number of metabolites directly after field sampling, even in BC and CM soils without previous atrazine treatment, by means of LC-MS/MS analyses. For atrazine degradation studies, all soils were incubated under different moisture conditions (50% maximum soil water-holding capacity (WHC(max))/slurried conditions). At the end of the incubation, the (14)C-atrazine mineralization was high in BS soil (81 and 83%) and also unexpectedly high in BC soil (40 and 81%), at 50% WHC(max) and slurried conditions, respectively. In CK soil, the (14)C-atrazine mineralization was higher (10 and 6%) than in CM soil (4.7 and 2.7%), but was not stimulated by slurried conditions. The results revealed that atrazine application history dramatically influences its degradation and mineralization. For the incubation period, the amount of extractable atrazine, composed of residues from freshly applied atrazine and residues from former field applications, remained significantly greater (statistical significance = 99.5 and 99.95%) for BS and CK soils, respectively, than the amount of extractable atrazine in the bulk field soils. This suggests that (i) mostly freshly applied atrazine is accessible for a complex microbial community, (ii) the applied atrazine is not completely mineralized and remains extractable even in adapted soils, and (iii) the microbial atrazine-mineralizing capacity strongly depends on atrazine application history and appears to be conserved on long time scales after the last application.     

How increasing availabilities of carbon and nitrogen affect atrazine behaviour in soils

 The effect of increasing amounts of glucose and mineral N on the behaviour of atrazine was studied in two soils. One had been exposed to atrazine under field conditions (adapted soil), the other had not (non-adapted soil), resulting, respectively, in an accelerated degradation of atrazine in the adapted soil and in a slow degradation of the herbicide in the non-adapted soil. The dissipation of ¹⁴C-atrazine via degradation and formation of non-extractable "bound" residues was followed during laboratory incubations in soils supplemented or not with increasing amounts of glucose and mineral N. In both soils, glucose added at rates of up to 16 g C kg^–1 soil did not modify atrazine mineralization but increased the formation of bound residues; this was probably due to the retention of atrazine by the growing microbial biomass. Atrazine dealkylation was enhanced when a large amount of glucose was added. In both soils, the addition of the largest dose of mineral N (2.5 g N kg^–1 soil) decreased atrazine mineralization. The simultaneous addition of glucose and mineral N enhanced their effects. When the largest doses of mineral N and glucose were added, atrazine mineralization stopped in both soils, and the proportion of bound residues increased. Glucose and mineral N additions influenced atrazine mineralization to a greater extent in the adapted soil than in the non-adapted one, as revealed by ANOVA, although glucose addition had a greater effect than N. The competition for space and nutrients between atrazine-degrading microorganisms and the total heterotrophic microflora probably contributed to the decrease in atrazine mineralization. Received: 9 June 1998     

Rapid development of enhanced atrazine degradation in a Dundee silt loam soil under continuous corn and in rotation with cotton

Mississippi Delta cotton (Gossypium hirsutum L.) production in rotation with corn (Zea mays L.) was evaluated in field experiments from 2000 to 2005 at Stoneville, Mississippi. Plots maintained under minimum tillage were established in 2000 on a Dundee silt loam with treatments including continuous cotton or corn and alternate cotton-corn rotations. Mineralization and dissipation of 14C [ring]-labeled atrazine were evaluated in the laboratory on soils collected prior to herbicide application in the first, second, third, and sixth years of the study. In soils collected in 2000, a maximum of 10% of the atrazine was mineralized after 30 days. After 1 year of herbicide application, atrazine-treated soils mineralized 52-57% of the radiolabeled atrazine in 30 days. By the sixth year of the study, greater than 59% of the atrazine was mineralized after 7 days in soils treated with atrazine, while soils from plots with no atrazine treatment mineralized less than 36%. The data also indicated rapid development of enhanced atrazine degradation in soils following 1 year of corn production with atrazine use. Atrazine mineralization was as rapid in soils under a rotation receiving biannual atrazine applications as in soils under continuous corn receiving annual applications of atrazine. Cumulative mineralization kinetics parameters derived from the Gompertz model (k and ti) were highly correlated with a history of atrazine application and total soil carbon content. Changes in the soil microbial community assessed by total fatty acid methyl ester (FAME) analysis indicated significant interactions of cropping system and sampling date, with FAME indicators for soil bacteria responsible for differences in community structure. Autoclaved soil lost all ability to mineralize atrazine, and atrazine-mineralizing bacteria were isolated from these plots, confirming the biological basis for atrazine mineralization. These results indicate that changes in degradative potential of a soil can occur rapidly and some changes in soil properties may be associated with cropping systems, which can contribute to enhanced atrazine degradation potential.     

Atrazine degradation in boreal nonagricultural subsoil and tropical agricultural soil

Purpose

The purpose of this study was to determine the natural atrazine degradation activity and the genetic potential in a soil profile spanning down to the groundwater zone, collected in Finland at a site where past use of atrazine has contaminated the groundwater, and in Indian agricultural topsoils having different histories of atrazine use.

Materials and methods

Atrazine degradation potential was assessed by quantifying the atrazine degradation genes atzA, trzN, and atzB by quantitative PCR reaction. Atrazine mineralization was studied by radiorespirometry in order to find out if these genes were expressed.

Results and discussion

Indian soils contained a large number up to 10⁴–10⁵ copies (g^?1 dry weight (dw) soil) of atrazine degradation genes after the first treatment with atrazine. These genes were also expressed, as up to 55 % of atrazine mineralized. Some unspecific binding of primers required thorough investigation and confirmation by sequencing of the qPCR products in the agricultural soil samples. The degradation capability of the nonagricultural boreal soil profile was much lower: atrazine degradation genes were present at detection limit (10² copies g^?1 soil), but mineralization studies indicated that these genes were not transcribed, since no or very little atrazine mineralization was observed.

Conclusions

Our results indicate that when atrazine was applied in agricultural practice, the soil atrazine degradation capacity was high. The organisms responsible for the degradation were effectively degrading atrazine already 3 months after the first treatment with atrazine. However, in boreal soil, decades after atrazine use had been discontinued, residual atrazine was not degraded even though a small number of degradation genes could still be detected in soil. There is a need for more specific primers for qPCR in tropical soils.     

阿特拉津在土壤, 矿物质及堆肥中的吸附, 运输和转化

Atrazine is a widely used herbicide for controlling weeds on both agricultural and nonagricultural land,which is equally detected in water supplies beyond safe concentrations.Although the presence of atrazine metabolites is an indication of herbicide degradation,some of them still exhibit toxicity,greater water solubility and weaker interaction with soil components than atrazine.Hence,studies with atrazine in the environment are of interest because of its potential to contaminate drinking water sources.Data on atrazine availability for transport,plant uptake,and microbial degradation and mineralization are therefore required to perform more comprehensive and realistic environmental risk assessments of its environmental fate.This review presents an account of the sorption-desorption phenomenon of atrazine on soil and other sorbents by revisiting the several mechanisms of atrazine-sorbent binding reported in the literature.The retention and transport of atrazine in soils;the influence of organic matter on atrazine sorption;the interactions of atrazine with humic substances,atrazine uptake by plants,atrazine bioccumulation and microbial degradation;atrazine transformation in composting environments;and finally atrazine removal by biosorption are discussed.     

Glufosinate and ammonium sulfate inhibit atrazine degradation in adapted soils

The co-application of glufosinate with nitrogen fertilizers may alter atrazine cometabolism, thereby extending the herbicide’s residual weed control in adapted soils. The objective of this study was to assess the effects of glufosinate, ammonium sulfate, and the combination of glufosinate and ammonium sulfate on atrazine mineralization in a Dundee silt loam exhibiting enhanced atrazine degradation. Application of glufosinate at rates of 10 to 40 mg kg⁻¹ soil extended the lag phase 1 to 2 days and reduced the maximum degradation rate by 15% to 30%. However, cumulative atrazine mineralization averaged 85% 21 days after treatment and was independent of treatment. Maximum daily rates of atrazine mineralization were reduced from 41% to 55% by application of 1 to 8 g kg⁻¹ of ammonium sulfate. Similarly, cumulative atrazine mineralization was inversely correlated with ammonium sulfate rates ranging from 1.0 to 8 g kg⁻¹ soil. Under the conditions of this laboratory study, atrazine degradation was relatively insensitive to exogenous mineral nitrogen, in that 8 g (NH₄)₂SO₄ per kilogram soil repressed but did not completely inhibit atrazine mineralization. Moreover, an additive effect on reducing atrazine mineralization was observed when glufosinate was co-applied with ammonium sulfate. In addition, ammonium fertilization alters the partitioning of ¹⁴C-atrazine metabolite accumulation and nonextractable residues, indicating that ammonium represses cleavage of the triazine ring. Consequently, results indicate that the co-application of glufosinate with N may increase atrazine persistence under field conditions thereby extending atrazine residual weed control in adapted soils.     

Comparison of the persistence of atrazine and metolachlor under field and laboratory conditions

A study was carried out in a loamy soil to evaluate the degradation of atrazine and metolachlor under laboratory-controlled and field-variable conditions as a function of temperature and soil moisture content. In laboratory trials, metolachlor showed fast degradation, with half-lives from 100 to 5.7 days in a temperature range from 5 to 35 degrees C at 100% of field capacity, whereas in the same conditions the degradation rate of atrazine was relatively slow, with half-lives from 407 to 23 days. Modeling of laboratory degradation data to predict field persistence was carried out. Field persistence of atrazine and metolachlor was measured in the same soil during the corn growing seasons in 1993, 1994, and 1996. In the three years the mean half-dissipation times for atrazine and metolachlor were 36 and 21 days, respectively. Calculations from model equations gave acceptable prediction of field dissipation of both herbicides. Limitations and perspectives of employed modelization procedure are discussed.     

Lumbricid macrofauna alter atrazine mineralization and sorption in a silt loam soil

Atrazine is a widely used herbicide and is often a contaminant in terrestrial and freshwater ecosystems. It is uncertain, however, how the activity of soil macrofauna affects atrazine fate and transport. Therefore, we investigated whether earthworms enhance atrazine biodegradation by stimulating herbicide degrading soil microflora, or if they increase atrazine persistence by facilitating herbicide sorption. Short (43 d) and medium term (86 d) effects of the earthworms Lumbricus terrestris and Aporrectodea caliginosa on mineralization, distribution, and sorption of U-ring-¹⁴C atrazine and on soil C mineralization was quantified in packed-soil microcosms using silt loam soil. A priming effect (stimulation of soil C mineralization) caused by atrazine supply was shown that likely lowered the earthworm net effect on soil C mineralization in atrazine-treated soil microcosms. Although earthworms significantly increased soil microbial activity, they reduced atrazine mineralization to ¹⁴CO₂-C from15.2 to 11.7% at 86 d. Earthworms facilitated formation of non-extractable atrazine residues within C-rich soil microsites that they created by burrowing and ingesting soil and organic matter. Atrazine sorption was highest in their gut contents and higher in casts than in burrow linings. Also, gut contents exhibited the highest formation of bound atrazine residues (non-extractable atrazine). Earthworms also promoted a deeper and patchier distribution of atrazine in the soil. This contributed to greater leaching losses of atrazine in microcosms amended with earthworms (3%) than in earthworm-free microcosms (0.003%), although these differences were not significant due to high variability in transport from earthworm-amended microcosms. Our results indicated that earthworms, mainly by casting activity, facilitated atrazine sorption, which increased atrazine persistence. As a consequence, this effect overrode any increase in atrazine biodegradation due to stimulation of microbial activity by earthworms. It is concluded that the affect of earthworms of atrazine mineralization is time-dependent, mineralization being slightly enhanced in the short term and subsequently reduced in the medium term.     

Mineralization of C-atrazine in an entic haplustoll as affected by selected winter weed control strategies

The effect of winter weed control (WWC) management on ¹⁴C-atrazine (6-chloro-N²-ethyl-N⁴-isopropyl-1,3,5-triazine-2,4-diamine) mineralization was investigated in an Entic Haplustoll in Argentina. Three WWC managements were selected: Chemical Fallow (CF) and Cereal Cover Crop (CCC), both under no-tillage, and Reduced Tillage (RT) with chisel and moldboard plow. Soil was sampled at two depths: 0–5 and 5–10 cm, to evaluate the soil stratification induced by the tillage system. To distinguish differences in atrazine degradation in soils with and without previous history of atrazine application two crop sequences were selected: continuous soybean [Glycine max L., Merr.] (CS) without previous atrazine exposure, and soybean–maize (Zea mays L.) rotation (SM) with atrazine application every winter and in alternate springs. The release of ¹⁴C-CO₂ during laboratory incubations of soils treated with ring labelled ¹⁴C-atrazine was determined. Soil organic matter (SOM) distribution was determined with depth and among three soil size fractions: 200–2000 μm, 50–200 μm and <50 μm. Previous atrazine application enhanced atrazine degrading microorganims. Atrazine mineralization was influenced by both WWC management and the tillage system. Chemical fallow showed the highest atrazine mineralization in the two crop sequences. Depth stratification in atrazine degradation was observed in the two WWC treatments under the no-tillage. Depth stratification in the content of soil organic C and relative accumulation of organic C in coarsest fractions (200–2000 and 50–200 μm) were observed mainly in no-till systems. Depth stratification of atrazine degrading activity was mainly correlated to the stratification of fresh organic matter associated with the coarsest fractions (200–2000 μm). Atrazine persistence in soil is strongly affected by soil use and management, which can lead to safe atrazine use through selection of appropriate agricultural practices.     

Effects of addition of maize litter and earthworms on C mineralization and aggregate formation in single and mixed soils differing in soil organic carbon and clay content

C mineralization and aggregate stability directly depend upon organic matter and clay content, and both processes are influenced by the activity of microorganisms and soil fauna. However, quantitative data are scarce. To achieve a gradient in C and clay content, a topsoil was mixed with a subsoil. Single soils and the soil mixture were amended with 1.0 mg maize litter C g soil⁻¹ with and without endogeic earthworms (Aporrectodea caliginosa). The differently treated soils were incubated for 49 days at 15 °C and 40% water holding capacity. Cumulative C mineralization, microbial biomass, ergosterol content and aggregate fractions were investigated and litter derived C in bulk soil and aggregates were determined using isotope analyses. Results from the soil mixture were compared with the calculated mean values of the two single soils. Mixing of soil horizons differing in carbon and clay content stimulated C mineralization of added maize residues as well as of soil organic matter. Mixing also increased contents of macro-aggregate C and decreased contents of micro-aggregate C. Although A. caliginosa had a stimulating effect on C mineralization in all soils, decomposition of added litter by A. caliginosa was higher in the subsoil, whereas A. caliginosa decreased litter decomposition in the soil mixture and the topsoil. Litter derived C in macro-aggregates was higher with A. caliginosa than with litter only. In the C poor subsoil amended with litter, A. caliginosa stimulated the microbial community as indicated by the increase in microbial biomass. Furthermore, the decrease of ergosterol in the earthworm treated soils showed the influence of A. caliginosa on the microbial community, by reducing saprotrophic fungi. Overall, our data suggest both a decrease of saprotrophic fungi by selective grazing, burrowing and casting activity as well as a stimulation of the microbial community by A. caliginosa.     

Estimation of sulfur mineralization and relationships with nitrogen and carbon in soils

A 25-week laboratory study was carried out to determine sulfur, carbon, and nitrogen mineralization rates in soil samples obtained from representative soils in France. Their relationship with some of the soil properties was investigated to find a predictor of mineralized S in soils. At 20°C and 80% water-holding capacity, the S mineralization rate ranged from 0.02 to 0.16 mg kg⁻¹ day⁻¹. It was significantly positively related to soil organic C and N and to C and N mineralization rates. It was weakly related to total soil S. The results suggest that the S mineralization is predominantly driven by heterotrophic microbial activity. A predictive equation for S mineralization based on soil C content, soil pH, and clay content is proposed.     

不同水分条件下添加白云石对酸性水稻土有机碳矿化的影响

施用石灰改良酸性土壤是常用的农艺措施之一。施用石灰影响土壤理化性质,进而影响土壤有机碳（Soil Organic Carbon,SOC）矿化。而SOC矿化与土壤肥力保持和有机碳库的大小存在紧密联系。因此,明晰施用石灰对酸性土壤有机碳矿化的影响具有重要的理论和现实意义。该研究以2种母质的酸性水稻土为对象,在50%、90%和130%土壤最大田间持水量（Water Holding Capacity,WHC）条件下添加和不添加白云石,再进行为期45 d的室内培养试验,探讨白云石和水分对SOC矿化的影响。研究结果表明,添加白云石显著影响2种土壤有机碳矿化速率,但白云石添加和水分的交互作用不显著。土壤含水量较低时（50% WHC）,2种土壤有机碳矿化均受到抑制。在较高土壤含水量情况下（90%～130% WHC）,白云石添加和水分的共同作用对SOC矿化的影响因土壤质地不同而异,淹水条件下（130% WHC）棕红壤有机碳矿化量高于湿润条件（90% WHC）,而红壤中的情况正好相反。白云石添加和水分均显著影响SOC累计矿化量,但二者交互作用仅在棕红壤中显著。添加白云石后,2种土壤pH值随着水分含量的增加而提高;土壤含水量较低时（50% WHC）,土壤pH值即可达到或接近目标值（pH值6.5）。这些结果表明,在评估施用白云石对SOC矿化的影响时,需要考虑土壤含水量和土壤本身的性质,以便为农业生产实践中合理施用白云石提供指导和建议。     

Influence of Jet‐Cooking Prowashonupana Barley Flour on Phenolic Composition,Antioxidant Activities,and Viscoelastic Properties

The objective was to study the influence of jet‐cooked Prowashonupana barley flour on total phenolic contents, antioxidant activities, water‐holding capacities, and viscoelastic properties. Barley flour was jet‐cooked without or with pH adjustment at 7, 9, or 11. Generally, the free phenolic content and antioxidant activity decreased after jet‐cooking, while the bound phenolic content and antioxidant significantly increased regardless of pH. Detectable levels of gallic acid, caffeic acid, ferulic acid, and p‐coumaroyl‐pentose in the jet‐cooked barley flour hydrolysates along with vitexin were found among 21 phenolics by LC‐ESI‐Q‐TOF‐MS analysis. Jet‐cooking at an elevated pH resulted in increased pasting viscosities. The oil content was decreased after jet‐cooking and continued to decrease with increased pH values. Jet cooking dramatically increased water holding capacity from 179% for unprocessed flour to 643% for jet‐cooked flour without pH adjustment, and water‐holding capacity was greatly increased to 914% by jet‐cooking at pH 11. The combination of jet‐cooking and pH adjustment had tremendous influence on water‐holding and pasting properties. This increase in functionality should contribute to food applications such as bakery and frozen products because of the release of the bound phenolic content, antioxidant activities, and improved water‐holding and pasting abilities.     

赣南飞播马尾松林林下植被盖度对土壤质量的影响

为了探明林下植被盖度对赣南飞播马尾松林土壤质量的影响,选取3种林下植被盖度（> 70%,30%～70%,< 30%）的林分,对其表层（0—10 cm）土壤理化性质的16个指标进行比较分析,筛选出土壤容重、田间持水量、土壤有机质、速效磷、pH值作为土壤质量评价指标,运用土壤理化综合指数评价不同林下植被盖度下土壤质量水平。结果表明:3种林下植被盖度间,土壤容重、土壤含水量、饱和持水量、毛管持水量、田间持水量、毛管孔隙度、总孔隙度、pH值存在显著差异（P < 0.05）;非毛管孔隙度、有机质、全氮、全磷、全钾、速效氮、速效磷、速效钾不存在显著差异（P > 0.05）。不同林下植被盖度的土壤理化综合指数存在极显著差异（P < 0.01）,土壤质量随着林下植被盖度的增加而得到显著提高。     

Movement,adsorption and mineralization of atrazine in two soils with and without switchgrass (Panicum virgatum) roots

A study was conducted to determine the influence of switchgrass roots on the mobility, adsorption–desorption and mineralization of atrazine in Cullen clay loam and Emporia loamy sand soils. Bromide and atrazine distribution profiles in the leachates indicated greater preferential movement in columns with roots than in columns without roots. Larger concentrations of atrazine were detected at lower depths of Emporia soil with switchgrass roots than without. Adsorption of atrazine was greater in Cullen than in Emporia soil and conformed to Freundlich isotherms. In both Cullen and Emporia soils, adsorption and desorption were not different between soil with or without switchgrass roots. After 84 days of incubation, less than 6% of the applied atrazine was mineralized in the Cullen soil and 2% in Emporia soil. Mineralization was greater in the Cullen soil than in the Emporia soil at 42, 56, 70 and 84 days of sampling. The presence of switchgrass roots did not affect the mineralization of atrazine in Emporia soil. The presence of switchgrass roots caused preferential movement of atrazine, but did not affect its adsorption and mineralization in either soil type.     

Atrazine mineralization in bulk soil and maize rhizosphere

An experiment was carried out in the greenhouse in order to compare atrazine mineralization in bulk soil and maize rhizosphere at different development stages. After 4, 8 and 12 weeks, we have (1) measured the soil microbial biomass C, (2) characterized the C substrate utilization profiles of the culturable microflora, and (3) analyzed atrazine mineralization. Microbial growth was stimulated in planted soil and different C substrate utilization patterns were obtained in bulk and rhizosphere soils during the first 2 months. During this period, laboratory tests for atrazine biodegradation revealed a lower mineralization potential in bulk than in planted soil. Atrazine mineralization was stimulated to a greater extent after atrazine application in the greenhouse but again the presence of plants had a favorable effect. After 12 weeks of cropping, the atrazine mineralization potential decreased in planted soil with or without prior atrazine application.     

不同耕法及秸秆还田对土壤水分运移变化的影响

[目的]探讨不同耕法与秸秆还田方式下,旱地草甸土土壤水分随深度运移的变化,为今后生产中因地制宜制定科学合理的耕作与培肥技术提供理论依据。[方法]采用田间定位试验,研究3种耕法免耕、浅翻、深翻与3种秸秆还田方式覆盖还田、浅翻还田、深翻还田条件下,作物生长不同时期、不同深度土层土壤含水量、田间持水量和容重的变化。[结果]土壤水分的年际间变化与降水量和降水变率有一定的关系。秸秆不还田条件下,连续2 a免耕,年际间土壤含水量随深度变化的特征曲线基本一致,0—20 cm耕层田间持水量降低13.62%,而浅翻与深翻分别增加11.32%和27.98%;耕翻深度对20—30 cm土层水分的影响较大,随作物生长和地表覆盖度增加,40 cm以下土层含水量的变化减弱。秸秆还田条件下,0—20 cm耕层浅翻还田与深翻还田田间持水量分别增加16.24%,5.08%,而土壤容重降低0.12,0.09 g/cm~3。[结论]同一耕法有秸秆还田处理土壤水分含量高于无秸秆还田,降水量越少,差异越明显。与免耕和免耕覆盖比较,翻耕与翻耕还田均增加了作物生长期间土壤含水量,提高了作物抗旱能力,产量有增加趋势。