Pharmacokinetics of minocycline in crucian carp (Carassius auratus) after intravenous and oral administration

The pharmacokinetic of minocycline was studied after a single intravenous as well as oral dose (5 mg/kg body weight) in crucian carp (Carassius auratus) reared in freshwater at 10°C. Plasma samples were randomly collected from six fish at each sampling time. Plasma concentrations were determined by high‐performance liquid chromatography and further subjected to noncompartmental analysis. Initial concentration of minocycline just after intravenous administration was calculated as 7.320 μg/ml, while the other parameters after intravenous injection were determined as flows: apparent elimination rate constant (λ_z) of 0.064 per hr, apparent elimination half‐life () of 10.82 hr, total body clearance (Cl) of 142.72 ml/hr/kg, volume of distribution (Vz) of 2,227.38 ml/kg and volume of distribution at steady‐state (Vss) of 1,937.08 ml/kg. While after oral administration, the λ_z, , mean absorption time (MAT), absorption half‐life (t_1/2ka) and bioavailability were determined as 0.059 per hr, 11.74, 5.55, 3.84 hr, and 81.98%, respectively, and the peak concentration was observed as 1.474 ± 0.362 μg/ml at 8 hr. It was shown that minocycline was slowly but relatively completely absorbed, extensively distributed, and slowly eliminated in crucian carp. Based on the ratios of AUC_0–24 hr/MIC90, a minocycline dosage of 5 mg/kg body weight administered intravenously or orally would be only effective to successfully treat crucian carp infected by bacterium with MIC values ≤0.25 μg/ml.     

Pharmacokinetics and tissue residues of vancomycin after oral administration in crucian carp

The pharmacokinetics and tissue residues of vancomycin in crucian carp (Carassius auratus) were studied after oral administration of a single dosage of 1 mg/kg body weight at water temperature of 18°C. The drug concentration‐time data were fitted using a two‐compartment open model with first‐order absorption. The vancomycin concentrations in plasma and tissues including muscle, liver and kidney were analyzed by means of liquid chromatography‐tandem mass spectrometry. As a result, the plasma absorption rate constant of vancomycin was 3.75/hr and the time point of maximum concentration in plasma was 3.0 hr respectively. The maximum concentration of vancomycin in plasma was 216.58 μg/L. The distribution half‐life and the elimination half‐life in plasma were 6.69 hr and 190 hr, as well as the absorption half‐life of plasma was 0.19/hr. The area under the plasma concentration‐time curve of plasma was 15.12 mg hr/L. The apparent volume of distribution of plasma was estimated to be 4.45 L/kg and the total body clearance was computed as 0.061 L hr^?1 kg^?1. In addition, the maximum tissue concentrations of vancomycin were in the order of liver > kidney > muscle. To guarantee the safety of tissue samples for consumption, it was suggested that the withdrawal time should not be less than 30 days at 18°C. All these results may provide guidance for future vancomycin treatment in aquaculture.     

葡萄糖、维生素C对普安银鲫早期发育的影响

为探究葡萄糖和维生素C（VC）对普安银鲫（Carassius auratus gibelio）早期发育的影响,采用静水溶液浸泡法研究了葡萄糖和VC溶液浸泡中普安银鲫仔鱼出膜时间、孵化率、成活率及卵黄囊仔鱼生长特点。结果显示,葡萄糖溶液质量浓度低于15 g·L^-1,仔鱼出膜时间随着葡萄糖质量浓度的增加而缩短,孵化率、成活率、体质量和全长则升高;葡萄糖溶液质量浓度高于15 g·L^-1对普安银鲫的早期发育表现出抑制作用。VC溶液质量浓度低于30mg·L^-1时,仔鱼出膜时间随着ρ（VC）的增加而降低,孵化率、成活率、体质量和全长则升高。ρ（VC）高于30mg·L^-1对普安银鲫的早期发育表现出抑制作用。研究表明适宜浓度的葡萄糖和VC能促进普安银鲫的早期发育。     

单剂量口灌阿维菌素在草鱼体内的药动学及残留研究

按0.1 mg/kg的剂量给草鱼(Ctenopharyngodon idellus)灌服阿维菌素,用高效液相色谱法检测用药后不同时间的血浆、肌肉和肝脏中的药物浓度,然后用3P97药代动力学软件处理药时数据,对药物在草鱼体内药动学及组织残留进行研究.结果表明:单剂量口灌阿维菌素在草鱼血浆中主要药动学参数为:AUC 1 6...     

Effects of a dietary administration of purple coneflower (Echinacea purpurea) on growth,antioxidant activities and 8 miRNAs expressions in crucian carp (Carassius auratus)

Echinacea purpurea (EP), a globally popular herbal medicine, has been used to treat various diseases in human and animals. However, little has been reported about its effects in fish. In this study, crucian carp (Carassius auratus) were selected to evaluate the effects of EP on growth performance and antioxidant response and the expressions of microRNAs. The results showed EP could stimulate the growth of crucian carp with the best effect was observed at dose of 4 g kg^?1. In serum, the content of hydroxyl radical (·OH) and malondialdehyde (MDA) decreased by EP supplementation, whereas the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) increased. Similarly, the content of ·OH decreased, and the activities of CAT, GPX and GR increased in liver of crucian carp. Furthermore, in livers of crucian carp, EP supplements upregulated the expressions of the microRNAs (miR‐16, miR‐21, miR‐125b, miR‐146a, miR‐155, miR‐181a and miR‐223), which had been confirmed to participate in regulating antioxidant and immune function in mammals. Our results suggest EP supplements in diets stimulated growth performance and antioxidant response of crucian carp. In liver, the upregulation of specific miRNAs may participate in the antioxidant effects of EP diets.     

Status of the crucian carp, Carassius carassius (L.), in the UK

The crucian carp, Carassius carassius (L.), is a small fish of restricted distribution in the UK, which is probably only native to central and eastern England. The crucian carp is endemic to northern Europe. It has been widely confused with the introduced and congeneric goldfish, Carassius auratus (L.), and forms hybrids with that species and the introduced Cyprinus carpio L. The status of this fish has become confused with the presence of the congener and its hybrids, and it is now thought to be less frequent in occurrence than formerly.     

水晶彩鲫、红鲫和锦鲤的腹膜脏层黑色素观察

对水晶彩鲫(Carassius auratus transparent coloredvar.)、红鲫(C.a.redvar.)和锦鲤(Cyprinus carpio ornamentalvar.)腹膜脏层黑色素进行肉眼观察和密度分布统计。结果表明,这3种鱼的不同体色个体,腹膜脏层黑色素的密度不同。依据黑色素的密度,将其分为Ⅰ~Ⅵ级。水晶彩鲫的腹膜脏层黑色素密度:肉白a个体为Ⅰ级(无黑色素分布),肉白b和红白个体多数在Ⅰ~Ⅱ级,红色个体多数在Ⅰ~Ⅳ级,杂色个体多数在Ⅳ~Ⅵ级,密度表现出依次递增的趋势。红鲫的腹膜脏层黑色素密度:白色个体分布在Ⅲ级,红白个体分布在Ⅲ~Ⅳ级,红色个体分布在Ⅲ~Ⅴ级,杂色分布在Ⅳ~Ⅵ级,青灰色分布在Ⅴ~Ⅵ级,密度依次也表现出递增的趋势。锦鲤的腹膜脏层黑色素密度:体表没有黑色斑纹的红、黄、白、红白和黄白等个体为Ⅰ级,无黑色素分布;体表有黑色斑纹的红黑、黄黑、白黑和红白黑等个体多数有黑色素出现,主要分布在Ⅳ级。[中国水产科学,2007,14(1):144-148]     

亚甲基蓝及其代谢物在异育银鲫体内分布及消除规律的研究

以15 mg·L~(-1)的亚甲基蓝浸泡异育银鲫(Carassius auratus gibelio)成鱼0.5 h后将其饲养于实验室水族箱中,研究了常规用药条件下亚甲基蓝及其代谢物在异育银鲫各组织中的分布及消除规律。采用高效液相色谱法检测亚甲基蓝及其代谢物在异育银鲫组织中的浓度,用DAS 3.0.5药物与统计软件对数据进行分析。结果表明:血液中亚甲基蓝、天青B、天青A达峰时间Tmax分别为第0.083、第0.5和第8小时,峰浓度值Cmax分别为143.436μg·L~(-1)、110.770μg·L~(-1)和8.444μg·L~(-1)。亚甲基蓝、天青B、天青A在异育银鲫4种组织中的浓度由高到低的顺序均为肝胰脏肾脏肌肉皮肤,其中异育银鲫肌肉和皮肤组织易蓄积亚甲基蓝及代谢物,至第64天仍可以检测到,组织中未检测到天青C。     

Predation as a determinant of size structure in populations of crucian carp (Carassius carassius) and tench (Tinea tinea)

Predation is an important structuring force in many fish assemblages and may have large, direct lethal effects on prey fish populations but also may affect growth rate and survival indirectly through shifts in behavioral patterns. Some species appear more sensitive to predation and earlier studies in a limited set of Finnish ponds suggests that crucian carp (Carassius carassius) is very sensitive to predation, which results in changes in population size-structure and density. In this study we tested the effect of piscivore presence or absence on crucian carp populations by using data from a large number (>600) of Swedish lakes across a wide geographical area and ranging in size from small ponds to large lakes (0.02–4400 ha). We also included a second species, tench (Tinea tinea), in our analyses. The population size structures of crucian carp and tench were clearly related to the presence or absence of piscivores. In lakes and ponds without piscivores. populations were dominated by small-bodied individuals. When piscivores were present, populations consisted almost exclusively of large individuals. Densities of crucian carp and tench were much higher in ponds and lakes without piscivores. There were no differences in size structure or relative abundance when catches from electrofishing were compared with gill net catches, suggesting that our results are not an effect of a behavioral shift in the presence of piscivores.     

彩鲫与红鲫杂交种体色遗传的初步研究

通过彩鲫与红鲫杂交、回交以及不同体色彩鲫（杂交种）自交、杂交,对其子代的体色性状进行了统计学分析。彩鲫与红鲫的体色遗传说明彩色受显性基因控制,红色受隐性基因控制;不同体色彩鲫自交、杂交,其后代的体色分离特性为肉色显性,红色隐性,亲本体色在后代中可以积累增加;肉色、红色彩鲫与眼睛颜色、闪光鳞数具遗传相关性。     

水晶彩鲫、红鲫、锦鲤、荷包红鲤杂交子代的生长和体色研究

利用人工授精的方法,进行水晶彩鲫、红鲫、锦鲤和荷包红鲤的相互杂交试验,测定各个杂交组合子代的成活率、生长速度和体色分离比例。结果表明：4种鱼能够相互杂交受精,孵出鱼苗。孵化率锦鲤自交最低为46．4％,其它组合为70％～80％;杂交鱼苗经28d的人工饲养,水晶彩鲫与荷包红鲤、锦鲤的正、反杂交,同其它杂交组合比较有明显的差别,其生长速度慢,个体之间差异大,成活率低;杂交子代的体型分为3类：鲫鱼型、鲤鱼型和鲤鲫型。鳞片反光组织(虹彩细胞或鸟粪素细胞)为2类：完全型、缺失型。体色分离复杂多样,水晶彩鲫与红鲫杂交是水晶彩鲫,红鲫与锦鲤、荷包红鲤杂交是青灰色鲤鲫杂种,水晶彩鲫与锦鲤、荷包红鲤杂交都会出现水晶彩色和青灰色鲤鲫杂种。     

New detection of Cyprinid herpesvirus 2 associated with mass mortality in colour crucian carp (Carassius auratus), in China

During October 2016, a mass mortality of colour crucian carp (Carassius auratus), which the affected fish were lethargic, inappetence and anoxic, was occurred in a fish farm located in Chengdu, Sichuan province, China. To elucidate the aetiology of this outbreak, histological and electron microscope examination, molecular investigation were conducted. Pathologic examination revealed multi foci necrosis on haematopoietic organs, gills, hearts and pancreas. Transmission electron microscopy observations exhibited sphere herpesvirus‐like particles distributed amongst the tissues of gill, spleen and kidney. Molecular analysis is verified that the causative agent of this outbreak was Cyprinid herpesvirus 2 (CyHV‐2). This report first report CyHV‐2 in colour crucian carp, which increases the concern about damage of CyHV‐2 and its potential role in species.     

诺氟沙星在鲫体内的药动学及残留研究

研究了单剂量肌肉注射和多剂量混饲口灌给药方式下,诺氟沙星在鲫(Carassius auratus)体内的药物动力学和残留情况。结果显示:在(25.4±0.3)℃水温条件下,以每千克鱼体重10 mg的剂量单次给鲫肌肉注射诺氟沙星后,药物几乎在瞬间吸收,0.0333 h时血液中达到6.6708μg/mL,其血药浓度-时间数据用一级吸收二室模型描述较为合适,主要的药动学参数为:t1/2α、t1/2β、AUC和C l(s)分别为:0.4231 h、9.1613 h、17.8619μg.h/mL和0.5727 L/(kg.h)。在(23±1)℃水温条件下,以每千克鱼体重10 mg的剂量多次连续5 d混饲后,鲫停药后第8天肌肉、血清和肝脏中未检测到药物,此时肾脏中药物浓度已降到(0.0463±0.0134)μg/g,低于0.05μg/g。建议在(23±1)℃水温条件下,按每千克体重10 mg的剂量连续5 d混饲给鲫诺氟沙星,休药期至少为最后一次给药后的8 d。     

异育银卿口服葡萄糖后血糖、血脂和肝糖原的变化

禁食 4周后对体重为 46± 5g的异育银鲫进行了葡萄糖耐量试验。口服 16 7mg·(10 0g) -1体重的葡萄糖之前及之后的 1、2、3、4、5、6、8、10、14、18和 2 4h分别抽血、取肝胰腺 ,分析血糖、血脂和肝糖原含量。实验结果表明 ,血糖在口服葡萄糖后 3h内迅速升高 ,并于 3h时达最高水平 ,以后逐渐下降。口服葡萄糖后 1h血脂含量最高 ,然后开始下降 ,并于 8h时又开始升高。口服葡萄糖后的最初 2h内肝糖原含量显著降低 ,随后开始提高 ,并在 6h时达最高峰。用体重为 (16 4± 12 )g的异育银鲫为材料禁食 4周后灌喂不同剂量的葡萄糖 ,研究葡萄糖剂量对血糖和肝糖原变化规律的影响。结果表明 ,随着口服葡萄糖剂量的增加 ,血糖升高幅度加大 ,而肝糖原含量则随葡萄糖剂量增加而减少。上述实验结果提示异育银鲫在胰岛素的分泌方面与哺乳类存在本质的不同。     

Body depth variation in crucian carp Carassius carassius: an experimental individual-based study

Abstract –  Induced morphological defences, where individuals develop morphological structures as a response to signals from sympatric predators, are common among invertebrates. Such defences have also been reported in a freshwater fish, the crucian carp Carassius carassius . In crucian carp, the presence of piscivorous fish presumably induces the development of a deep body as a defence mechanism. We tested how the growth rate (in body depth, length and mass) of individually tagged crucian carp responds to pike Esox lucius odours and control water. In general, crucian carp exposed to pike odour grew better (both in length and mass) than control fish. The treatment crucian carp also developed deeper bodies than control crucian carp. Further, there was a strong positive relationship between individual growth rate and the rate of change in body depth and this relationship did not differ between control and treatment fish.     

银鲫与普通鲫群体遗传结构的比较分析

利用15个微卫星多态性分子标记,对黑龙江省方正县双龙水库和双凤水库两个水体的雌核发育银鲫(Car-assius auratus gibelio)和二倍体普通鲫(Carassius auratus(L))的群体遗传多样性进行比较分析。结果显示:平均每个多态性位点获得银鲫10.39个有效等位基因,普通鲫11.82个有效等位基因;双龙水库三倍体银鲫和二倍体普通鲫的平均观测杂合度分别为0.6035和0.6300,双凤水库中的三倍体银鲫和二倍体普通鲫的平均观测杂合度为0.5311和0.5885;相似性与遗传距离分析结果显示:相似性最大的是双凤水体内的银鲫和普通鲫(0.8127),远大于两个水体间的银鲫群体间的相似性。结果表明,银鲫与普通鲫存在着明显的遗传物质交流,较高的遗传相似性不仅与传统的银鲫起源于普通鲫的观点一致,也暗示银鲫与普通鲫存在经常性地遗传物质交流。     

三倍体湘云鲫及其亲本线粒体DNA的比较研究

用人工选育的异源四倍体鲫鲤（♂）与白鲫（♀）杂交获得具有明显生长优势的三倍体湘云鲫。采用差速离心和核酸酶处理等方法，从三倍体、白鲫和异源四倍体鲫鲤肝组织中提取线粒体DNA，并用9种限制性内切酶进行单酶酶切分析。经琼脂糖凝胶电泳后计算出各酶切片段的大小，测得三倍体、白鲫和异源四倍体鲫鲤mtDNA的分子大小分别为16．24kb、16．60kb和16．20kb。根据各单倍型间的酶切片段共享度，估算出3个群体间的遗传距离，说明了mtDNA母系遗传的特性。     

Molecular cloning of CD4 from ginbuna crucian carp <Emphasis Type="Italic">Carassius auratus langsdorfii</Emphasis>

ABSTRACT:   The clonal triploid ginbuna crucian carp Carassius auratus langsdorfii , a naturally occurring gynogenetic fish, is a useful model for studying T-cell-mediated immunity. CD4, a T-cell receptor (TCR) coreceptor, is a membrane-bound glycoprotein found on helper T-cells, and assists in the binding of major histocompatibility complexes. In the present study, full-length cDNAs encoding the CD4 molecule from the S3n strain of ginbuna crucian carp were cloned and characterized. 5'-rapid amplification of cDNA ends (RACE) and 3'-RACE yielded two distinct cDNA clones of CD4 homolog from the ginbuna, and these sequences share 95% identity at the amino acid level. These ginbuna CD4 molecules consisted of a signal peptide, immunoglobulin superfamily (IgSf) like domains, a transmembrane domain, and a cytoplasmic domain similar to other known CD4. A tyrosine protein kinase p56^lck binding motif is conserved in the cytoplasmic tail of ginbuna CD4. Phylogenetic tree analysis indicated that ginbuna CD4 sequences are closely related to CD4L-1 from other fish species. Expression of ginbuna CD4 mRNA was detected in the gill, thymus, head kidney, trunk kidney and peripheral blood leukocytes, indicating that its expression pattern is similar to that of ginbuna TCRβ mRNA. The results suggest that ginbuna CD4 sequences are useful as molecular probes for helper T-cells.     

急性氨氮胁迫对淇河鲫幼鱼脑、鳃、肝、肾组织结构的影响

随着养殖密度的增加,过量投喂产生的残饵和大量代谢废物在水中积累,并通过氨化作用转化为氨态氮在水体中富集,对鱼类的抗氧化系统、呼吸及神经系统均存在较大的危害。本研究以6月龄淇河鲫（）幼鱼[体重（15±2）g]为实验对象,研究水体中过量氨氮（50 mg/L、100 mg/L、150 mg/L、200 mg/L、250 mg/L、300 mg/L）对淇河鲫脑、鳃、肝、肾的组织学损害。结果发现,淇河鲫安全养殖的氨氮浓度为16.6 mg/L,超过安全浓度后随着氨氮浓度的升高及氨氮胁迫时间的延长,脑神经纤维结构被破坏的程度增大,神经细胞体出现不同程度坏死;鳃小片也出现不同程度的萎缩聚拢,鳃丝基部充血肿大,鳃丝间隔增大,上皮细胞排列紊乱,泌氯细胞和上皮细胞出现不同程度的空泡化,超显微观察发现,鳃小片出现萎缩,上皮细胞脱落,细胞表面分泌物增多;肝组织中心静脉周围肝细胞排列出现不同程度的紊乱,肝小叶轮廓模糊不清且间距增大,肝细胞排列疏松,细胞核出现不同程度的偏移、溶解,细胞表面粗糙且分泌物增多;肾组织出现淋巴细胞浸润和扩散、肾小囊腔膨大、肾小管腔缩小的现象,甚至出现了肾小球坏死。以上结果表明高浓度氨氮对淇河鲫幼鱼的神经、呼吸及代谢相关器官均存在不同程度的损害。因而,在淇河鲫大规模养殖过程中,应严格监测水体中氨氮浓度,践行绿色、健康养殖模式。