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乳铁蛋白生理功能研究现状 总被引:10,自引:0,他引:10
乳铁蛋白是一种具有多种生理功能的铁结合糖蛋白。本文阐述了乳铁蛋白促进铁吸收、调节免疫、抗菌、抗病毒、阻断氧自由基和促进双歧杆菌生长等生理功能,并对其应用前景进行了展望。 相似文献
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乳铁蛋白的营养生理作用及其作为饲料添加剂的应用 总被引:3,自引:0,他引:3
对乳铁蛋白的乳铁蛋白活性多太的结构及其动物体内的分布进行了分析,重点阐述了它们促进肠道铁离子的吸收,调节机体免疫机能,抑菌和抗菌活性,抗病毒,抗氧化,刺激肠道有益菌群生长,保护肠道等多种生理功能,总结了乳铁蛋白的测定方法,讨论了利用天然初乳制备乳铁蛋白和乳铁蛋白活性肽的的工艺和利用基因工程生产乳铁蛋白和乳铁蛋白活性肽。对乳铁蛋白和乳铁蛋白活性肽在饲料加工过程及动物肠道中的稳定性进行了分析,并初步讨论了乳铁蛋白和乳铁蛋白活性肽代替饲用抗生素作为饲料添加剂的应用前景。 相似文献
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为了有助于检测市场上乳及乳制品中的乳铁蛋白含量,本试验建立了定量测定乳及乳制品中乳铁蛋白的SDS-PAGE电泳分析法.试验采集了10种液态奶、3种乳清粉、3种干酪、4种奶粉和4种酸奶,利用SDS-PAGE法分离乳铁蛋白,并优化了分离胶的浓度、电压、上样量和染色法.SDS-PAGE法的最佳条件为:分离胶浓度12%,浓缩胶电压120V,分离胶电压200V,改良的考马斯亮蓝染色,乳铁蛋白标准品的点样浓度0.1111mg/mL.点样量12μL.乳清粉及奶粉比干酪、液态奶和酸奶中的乳铁蛋白含量高,并且,液态奶、干酪和奶粉的检出限分别为2.9、29.0和29.0mg/100g,液态奶、干酪和奶粉的定量限分别为9.7、97.0和97.0mg/100g.该条件下测定乳及乳制品中乳铁蛋白含量的RSD分别为0.01%-8.70%.说明利用本文建立的SDS-PAGE电泳法简易、快速、准确,并且可以作为定量测定乳及乳制品中乳铁蛋白的方法. 相似文献
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乳铁蛋白在猪病防治及饲料中的应用 总被引:1,自引:0,他引:1
本文概述了乳铁蛋白的分子结构及其生物学功能,从促进铁的吸收、调节免疫、抗菌、抗病毒、抗氧化等生理功能方面说明其在仔猪疾病防治及饲料中的应用前景。 相似文献
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转基因动物产品-人乳铁蛋白与测定方法 总被引:4,自引:0,他引:4
乳铁蛋白是一种分子量为76.386kDa的结合性糖蛋白,在其多肽链上有两条碳水化合物侧链,乳铁蛋白存在于人乳及牛乳中,乳腺为主要分泌器官,乳铁蛋白具有多种重要的生理功能,在多种免疫细胞表面存在乳铁蛋白受体,编码人乳铁蛋白的基因全长2.358kb,由其释译的最初乳铁蛋白为711个氨基酸残基,成熟乳铁蛋白为692个氨基酸残基,人乳铁蛋白的转基因研究已获成功,获得表达,并初步应用于临床治疗,乳铁蛋白的检测方法包括酶联免疫吸附试验(ELISA),放射免疫(RIA),免疫卵迹(Western-Blot)及免疫组化方法,还可应用PCR和核酸探针技术对人乳铁蛋白转基因肉品进行检测。 相似文献
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Huang YQ Morimoto K Hosoda K Yoshimura Y Isobe N 《Veterinary immunology and immunopathology》2012,145(1-2):499-504
Lingual antimicrobial peptide (LAP), one of the β-defensins in bovines, and lactoferrin (LF) are synthesized in mammary epithelium and have bactericidal and bacteriostatic functions. However, it is not known whether they have similar expression patterns. Therefore, the present study was undertaken to compare (1) immunolocalization of LAP and LF in the mammary gland and (2) changes in concentration of these two components in milk after lipopolysaccharide (LPS) challenge. Bovine mammary tissues without LPS challenge were collected and their sections were immunostained with antibodies to LAP or LF. Milk from our previous study was collected every hour up to 12h and twice daily from d 1 to 7 after LPS challenge (the day of infusion was considered as d 0). These milk samples were measured for LAP but not LF in our previous report. Therefore, concentration of LF was measured by enzyme immunoassay in the present study. Epithelial cells of some alveoli showed immunopositive reaction for LF, but negative for LAP. Conversely, some alveoli were LAP positive in their epithelial cells but LF negative. Many alveoli had immunoreactions for neither LAP nor LF. The concentration of LAP in milk was elevated significantly at 3h after LPS infusion compared with pre-infusion values and remained at a high level until 12h. However, LF concentration in milk remained low at d 0 and increased at d 2. These results suggest that LAP and LF were mostly differentially localized in the alveolar epithelium in mammary glands. The different spatial expressions between them may be associated with their different temporal expression mechanisms. 相似文献
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近年来,随着对乳铁蛋白和乳铁蛋白肽作用机制和空间结构的研究趋于成熟,国内外研究者逐渐将研究重点倾向于乳铁蛋白和乳铁蛋白肽的开发应用.主要介绍了乳铁蛋白和乳铁蛋白肽基因工程的研究进展,尤其是对原核表达系统和真核表达系统进行了综述. 相似文献
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乳铁蛋白是一种存在于哺乳动物外分泌液中的非血红素铁结合性糖蛋白,它具有多种生物活性功能.本文主要就乳铁蛋白的生物学功能及其在仔猪中生产中的应用前景做一综述. 相似文献