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1.
Kikuzaki H Kayano S Fukutsuka N Aoki A Kasamatsu K Yamasaki Y Mitani T Nakatani N 《Journal of agricultural and food chemistry》2004,52(2):344-349
Four new abscisic acid related compounds (1-4), together with (+)-abscisic acid (5), (+)-beta-D-glucopyranosyl abscisate (6), (6S,9R)-roseoside (7), and two lignan glucosides ((+)-pinoresinol mono-beta-D-glucopyranoside (8) and 3-(beta-D-glucopyranosyloxymethyl)-2- (4-hydroxy-3-methoxyphenyl)-5-(3-hydroxypropyl)-7-methoxy-(2R,3S)-dihydrobenzofuran (9)) were isolated from the antioxidative ethanol extract of prunes (Prunus domestica L.). The structures of 1-4 were elucidated on the basis of NMR and MS spectrometric data to be rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo[3,2,1]oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid (1), rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo[3,2,1]oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid 3'-O-beta-d-glucopyranoside (2), rel-5-(1R,5S-dimethyl-3R,4R,8S-trihydroxy-7-oxa-6-oxobicyclo[3,2,1]oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid (3), and rel-5-(1R,5S-dimethyl-3R,4R,8S-trihydroxy-7-oxabicyclo[3,2,1]- oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid (4). The antioxidant activities of these isolated compounds were evaluated on the basis of oxygen radical absorbance capacity (ORAC). The ORAC values of abscisic acid related compounds (1-7) were very low. Two lignans (8 and 9) were more effective antioxidants whose ORAC values were 1.09 and 2.33 micromol of Trolox equiv/micromol, respectively. 相似文献
2.
Nutrients and antioxidant molecules in yellow plums (Prunus domestica L.) from conventional and organic productions: a comparative study 总被引:1,自引:0,他引:1
Lombardi-Boccia G Lucarini M Lanzi S Aguzzi A Cappelloni M 《Journal of agricultural and food chemistry》2004,52(1):90-94
Yellow plums (Prunus domestica L) conventionally and organically grown in the same farm were selected to study the influence of different agronomic practices on antioxidant vitamins (ascorbic acid, vitamin E, beta-carotene) and phenolics (total polyphenols, phenolic acids, flavonols) concentration. Conventional plums were grown on tilled soil. Three organic cultivations were performed: tilled soil, soil covered with trifolium, and soil covered with natural meadow. Differences in macronutrients were marginal, whereas antioxidant vitamins and phenolic compounds concentration markedly differed among cultivations. Ascorbic acid, alpha-, gamma-tocopherols, and beta-carotene were higher in organic plums grown on soil covered with natural meadow. The highest phenolic acids content was detected in plums grown on soil covered with trifolium. Total polyphenols content was higher in conventional plums. Quercetin was higher in conventional plums, but myrecitin and kaempferol were higher in organic plums. Under the same cultivar and climate conditions, the type of soil management turned out of primary importance in influencing the concentration of health-promoting compounds. 相似文献
3.
Kayano S Kikuzaki H Fukutsuka N Mitani T Nakatani N 《Journal of agricultural and food chemistry》2002,50(13):3708-3712
Ethanol extract of prune was separated into hexane-soluble and H(2)O-soluble fractions, and the H(2)O-soluble fraction was further separated into a methanol (MeOH) eluate and an H(2)O eluate by Diaion HP-20 column chromatography. The MeOH eluate exhibited the strongest antioxidant activity among the separated fractions evaluated by oxygen radical absorbance capacity (ORAC). Further purification of the MeOH eluate led to isolation of a novel compound, 4-amino-4-carboxychroman-2-one, together with four known compounds (p-coumaric acid, vanillic acid beta-glucoside, protocatechuic acid, and caffeic acid), structures of which were identified by NMR and MS analyses. The ORAC values of these isolated compounds showed 0.15-1.43 units (micromol of Trolox equiv)/micromol, and the new compound showed a remarkable synergistic effect on caffeoylquinic acid isomers. The antioxidant activity of the MeOH eluate was highly dependent on the major prune components, caffeoylquinic acid isomers, with a contribution from the new synergist. 相似文献
4.
Davis DR 《Journal of agricultural and food chemistry》2007,55(10):4278; author reply 4279-4278; author reply 4278
5.
《Communications in Soil Science and Plant Analysis》2012,43(7):561-571
Abstract Fall trunk injections of 200 g K in K2SO4 solution or up to 300 g K in K2HPO4 solution had no effect on sweet cherry mid‐shoot leaf K in August. Phosphorus content was significantly increased in buds in March and midshoot leaves in August one year by fall injection of K2HPO4 (79.2 g P). Spring trunk injections of 12.5 g K (K2S04 or K2HPO4) in prune increased leaf K within four days and K levels remained higher than controls for at least 22 days with K2SO4 Leaf P was increased within four days by K2HPO4 and was higher than controls in August. Fruit set in sweet cherry was significantly lower on trees injected with 200 g K (K2S04) and 300 g K (K2HPO4). Yield was significantly lower for all injection treatments and was probably caused at least in part by reduced fruit set. Fruit quality was not affected by K injections. 相似文献
6.
《Communications in Soil Science and Plant Analysis》2012,43(7):545-560
Abstract Potassium sulfate was applied to established, non‐irrigated, K deficient trees on fine textured soil by banding, placing in augered holes and injecting into the soil. Additional trees received a heavy compost mulch in early fall. Trenches were dug in the fall beside trees to break roots and then were backfilled with K2SO4, dolomite lime or a combination of the two. One percent K solutions of KNO3 or K2SO4 were sprayed on trees four times during the growth season. Sweet cherry trees only responded to compost mulch applications within two years. August midshoot leaf K, leaf N and fruit size were increased within one year however tree growth and yield were not affected. Fruit size and shoot growth were partially dependent on August leaf K level. August midshoot leaf K of prune trees was increased within one growing season by all treatments except trench plus lime. Trees receiving compost also had increased levels of leaf N, P, Ca, Mn, Fe, Cu and B. Yield and shoot growth were increased only by compost mulch applications. Fruit size was partially dependent on August leaf K levels. 相似文献
7.
Carbonaro M Mattera M Nicoli S Bergamo P Cappelloni M 《Journal of agricultural and food chemistry》2002,50(19):5458-5462
Despite the increasing interest in organic products, knowledge about how different levels of fertilization affect nutritionally relevant components is still limited. The concentration of polyphenols and the activity of polyphenoloxidase (PPO), together with the content in ascorbic acid, citric acid, and alpha- and gamma-tocopherol, were assayed in conventional and organic peach (Prunus persica L., cv. Regina bianca) and pear (Pyrus communis L., cv. Williams). 2-Thiobarbituric acid reactive substances and the tocopherolquinone/alpha-tocopherol ratio were used as markers of oxidative damage in fruits. A parallel increase in polyphenol content and PPO activity of organic peach and pear as compared with the corresponding conventional samples was found. Ascorbic and citric acids were higher in organic than conventional peaches, whereas alpha-tocopherol was increased in organic pear. The concentration of oxidation products in organic samples of both fruits was comparable to that of the corresponding conventional ones. These data provide evidence that an improvement in the antioxidant defense system of the plant occurred as a consequence of the organic cultivation practice. This is likely to exert protection against damage of fruit when grown in the absence of pesticides. 相似文献
8.
L. Wang S. Vestrheim 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(4):143-146
Sour cherry (Prunus cerasus L.) variety 'English Morello' fruits were stored for up to 20 days at 2°C in air or controlled atmosphere (CA) consisting of 25% CO2+10% O2, 15% CO2+10% O2, or 5% CO2+10% O2. The effects were compared on decay, juice optical density, titratable acidity, pH, soluble solids content and firmness. CA treatment has a great potential for sour cherry storage in maintaining quality and in extending the length of the storage period. The study has indicated that the decay of sour cherry can be greatly reduced by 25% CO2. High CO2 concentration gave the best retention of red colour and titratable acidity throughout the storage period. Fruit juice optical density and firmness increased with storage time. Fruits stored in 25% CO2+10% O2 had higher soluble solids content compared with fruits stored in air. 相似文献
9.
L. Wang S. Vestrheim 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(4):136-142
Sweet cherry (Prunus avium L.) cultivars 'Emperor Francis', 'Huldra', 'Sam', 'Stella', 'Kristin', and 'Van' were stored in controlled atmosphere (CA) during one, two or three seasons. The cherries were held at a temperature of 0°C, or 2°C for up to three weeks. The fruit quality was evaluated on juice optical density (OD), titratable acidity (TA), pH, soluble solids content (SSC), ethanol, firmness and flavour. In general, CA had little effect on SSC in the three seasons and did not show any effects in reducing pitting. Controlled atmosphere has shown some beneficial effects on flavour, reducing decay, reducing toughening, and maintaining TA in sweet cherries. Generally, flavour score was higher for fruit kept in CA than for fruit stored in air, and the higher the CO2 the better the taste. 'Van' and 'Sam' showed excellent taste scores after three weeks both from CA and air storage. There was very little ethanol accumulation in the fruits under CA, and it was well below the detrimental level. It is recommended that O2 levels for sweet cherry storage can be further reduced below 10% and/or CO2 can be increased above 15%. 相似文献
10.
An on-line HPLC-DPPH screening method for phenolic antioxidants in apple methanol/water (80:20, v/v) extracts was applied. The determination of antioxidants was based on a decrease in absorbance at 515 nm after postcolumn reaction of HPLC-separated antioxidants with the 2,2'-diphenyl-1-picrylhydrazyl radicals (DPPH*). Each of the antioxidants separated by the HPLC column was observed as a negative peak corresponding to its antioxidative activity. The on-line method was applied for quantitative analysis of the antioxidants. A linear dependence of negative peak area on concentration of the reference antioxidants was observed. For validation of the on-line method the limit of detection, LOD (microg/mL), and the limit of quantification, LOQ (microg/mL), of the phenolic compounds were determined. Comparison of the UV and DPPH radical quenching chromatograms with authentic compounds identified catechin, chlorogenic acid, caffeic acid, epicatechin, and phloridzin in the apple cultivars (Lobo, Golden Delicious, and Boskoop), and the distribution of total antioxidant activity was calculated. 相似文献
11.
A group of 28 sweet cherry (Prunus avium L.) local cultivars from the Jerte Valley in Western Spain were fingerprinted using microsatellites and their self-incompatibility alleles determined using S-RNase conserved primers. A total of 12 microsatellite primer pairs revealed 42 informative alleles that allowed the unequivocal identification of 26 genotypes. UPGMA cluster analysis of the similarity data produced a clear separation between the old traditional cultivars and the most recent introductions. The S-allele analysis showed a low number of different alleles in the older cultivars studied and revealed the presence of three new S-alleles not described previously. The combined information obtained from SSR fingerprinting and S-allele identification will be useful to optimize the conservation of the cherry genetic resources present in the area. Moreover, this approach can be applied to optimize the conservation of local genetic resources of other fruit tree species. 相似文献
12.
13.
Ruiz D Egea J Gil MI Tomás-Barberán FA 《Journal of agricultural and food chemistry》2005,53(24):9544-9552
Thirty-seven apricot varieties, including four new releases (Rojo Pasión, Murciana, Selene, and Dorada) obtained from different crosses between apricot varieties and three traditional Spanish cultivars (Currot, Mauricio, and Búlida), were separated according to flesh color into four groups: white, yellow, light orange, and orange (mean hue angles in flesh were 88.1, 85.0, 77.6, and 72.4, respectively). Four phenolic compound groups, procyanidins, hydroxycinnamic acid derivatives, flavonols, and anthocyanins, were identified by HPLC-MS/MS and individually quantified using HPLC-DAD. Chlorogenic and neochlorogenic acids, procyanidins B1, B2, and B4, and some procyanidin trimers, quercetin 3-rutinoside, kaempferol 3-rhamnosyl-hexoside and quercetin 3-acetyl-hexoside, cyanidin 3-rutinoside, and 3-glucoside, were detected and quantified in the skin and flesh of the different cultivars. The total phenolics content, quantified as the addition of the individual compounds quantified by HPLC, ranged between 32.6 and 160.0 mg 100 g(-1) of edible tissue. No correlation between the flesh color and the phenolic content of the different cultivars was observed. 相似文献
14.
Tesoriere L Butera D Gentile C Livrea MA 《Journal of agricultural and food chemistry》2007,55(21):8465-8471
An increasing body of evidence on the association between adherence to the Mediterranean diet and healthy status is being accumulated. Floral buds of Capparis spinosa L. are commonly used in the Mediterranean cuisine as flavoring for meat and other foods. The present study evaluated bioactive components and antioxidant activity of Sicilian capers stabilized in salt. Whereas alpha-tocopherol was absent, low levels of gamma-tocopherol and vitamin C were measured. With reference to one serving size (8.6 g of capers), rutin was 13.76 mg, isothiocyanates, recently acknowledged as anticarcinogen phytochemicals, were 42.14 micromol, total phenols were 4.19 mg of gallic acid equivalents (GAE), and the total antioxidant potential measured using the [2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] diammonium salt (ABTS) cation radical decolorization assay was 25.8 micromol of Trolox equivalents. The antioxidative activity of a caper hydrophilic extract was assessed in a number of assays. The extract at 3.5 and 7.0 microM GAE exhibited a dose-dependent peroxyl radical scavenging activity in a methyl linoleate methanol solution oxidized by azo initiator, and reduced hypervalent iron myoglobin species formed from met-Mb an H 2O 2, at 180 microM GAE. The hydrophilic extract, at 70-280 microM GAE, caused a dose-dependent inhibition of lipid autoxidation in heated red meat, incubated with simulated gastric fluid for 180 min. In the same model rutin tested at a concentration corresponding to its content in the extract was ineffective, and alpha-tocopherol at 25 microM was poorly effective. The hydrophilic extract (70 microM GAE) prevented the consumption of the co-incubated alpha-tocopherol, whereas lipid oxidation was inhibited for the experimental time, suggesting cooperative interactions between extract components and the vitamin. The findings encourage the use of caper with foods that contribute oxidizable lipids in view of the association between dietary oxidized lipids and risk of oxidative stress-based diseases. 相似文献
15.
Drogoudi PD Vemmos S Pantelidis G Petri E Tzoutzoukou C Karayiannis I 《Journal of agricultural and food chemistry》2008,56(22):10754-10760
Fruit physical and chemical characters of 29 apricot cultivars of Greek and American origin and their hybrids were evaluated using correlation and principal component analysis. A remarkable variation was observed in the total phenol content (0.3-7.4 mg gallic acid equivalent g(-1) FW) and total antioxidant capacity (0.026-1.858 mg ascorbic acid equivalent g(-1) FW), with the American origin cultivars Robada and NJA(2) and the new cultivar Nike exhibiting the greatest values. The cultivar Tomcot and hybrid 467/99 had the highest content of total carotene (37.8 microg beta-carotene equivalent g(-1) FW), which was up to four times greater as compared with the rest of studied genotypes. The dominant sugar in fruit tissue was sucrose, followed second by glucose and third by sorbitol and fructose-inositol. The new cultivars Nike, Niobe, and Neraida contained relatively higher contents of sucrose and total sugars, while Ninfa and P. Tirynthos contained relatively higher contents of K, Ca, and Mg. Correlation analysis suggested that late-harvesting cultivars/hybrids had greater fruit developmental times (r = 0.817) and contained higher sugar (r = 0.704) and less Mg contents (r= -0.742) in fruit tissue. The total antioxidant capacity was better correlated with the total phenol content (r = 0.954) as compared with the total carotenoid content (r = 0.482). Weak correlations were found between the fruit skin color and the antioxidant contents in flesh tissue. Multivariate analysis allowed the grouping of variables, with more important variables being the harvest date, fruit developmental time, skin Chroma, sorbitol, and total sugar, K and Mg contents. Plotting the genotypes in a dendrogram revealed cases of homonymy between parents and hybrids, although independent segregation of the measured traits after hybridization was also found. 相似文献
16.
Nakatani N Kayano S Kikuzaki H Sumino K Katagiri K Mitani T 《Journal of agricultural and food chemistry》2000,48(11):5512-5516
Neochlorogenic acid (3-CQA) and cryptochlorogenic acid (4-CQA), isolated from prune (Prunus domestica L.), were identified by NMR and MS analyses. In addition, the quantity of chlorogenic acid isomers in prune were measured by HPLC. These isomers, 3-CQA, 4-CQA, and chlorogenic acid (5-CQA), were contained in the ratio 78.7:18. 4:3.9, respectively. 4-CQA was identified and quantified in prune for the first time, and relatively high amounts of this isomer were characteristic. Antioxidative activities of the chlorogenic acid isomers, such as scavenging activity on superoxide anion radicals and inhibitory effect against oxidation of methyl linoleate, were also evaluated. Each isomer showed antioxidative activities which were almost the same. 相似文献
17.
Pino JA Mesa J Muñoz Y Martí MP Marbot R 《Journal of agricultural and food chemistry》2005,53(6):2213-2223
The volatile components of 20 mango cultivars were investigated by means of simultaneous distillation-extraction, GC, and GC-MS. Three hundred and seventy-two compounds were identified, of which 180 were found for the first time in mango fruit. The total concentration of volatiles was approximately 18-123 mg/kg of fresh fruit. Terpene hydrocarbons were the major volatiles of all cultivars, the dominant terpenes being delta-3-carene (cvs. Haden, Manga amarilla, Macho, Manga blanca, San Diego, Manzano, Smith, Florida, Keitt, and Kent), limonene (cvs. Delicioso, Super Haden, Ordonez, Filipino, and La Paz), both terpenes (cv. Delicia), terpinolene (cvs. Obispo, Corazon, and Huevo de toro), and alpha-phellandrene (cv. Minin). Other qualitative and quantitative differences among the cultivars could be demonstrated. 相似文献
18.
The distribution of cadmium- (Cd-) binding components in flaxseed (cultivar NorMan) containing 0.526 ppm (ng/mg) Cd was investigated. Proteins extracted from dehulled, defatted flaxseed were fractionated by anion-exchange and size-exclusion chromatography. The contents of Cd and other metals, UV/visible spectral characteristics, and amino acid compositions of these fractions were analyzed. Over 66% of the eluted Cd was recovered by 0.1 M NaCl elution from DEAE-Sephacel, in a thiol-rich fraction representing only 7% of the extracted proteins. Sephadex G50 size-exclusion chromatography of this 0.1 M NaCl fraction concentrated most of the Cd in a low-molecular-weight peak eluting at V(t). About 72% of the extracted flaxseed proteins eluted from DEAE-Sephacel at 0.25 M NaCl and contained only 25% of the eluted Cd. Because the major Cd-binding fraction is a minor constituent of flaxseed, these results indicate the potential to isolate flaxseed's major storage protein with a low Cd content. 相似文献
19.
Sauris Panda Juan Pedro Martín Itziar Aguinagalde 《Genetic Resources and Crop Evolution》2003,50(5):489-495
The chloroplast DNA of 96 sweet cherry cultivars (Prunus avium L.) and five cultivars of sour cherry (P. cerasus L.) were analysed to reveal their haplotypes using PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) method. The main advantages of the PCR-RFLP technique are: resolutive, time and cost effective and reproducible. Approximately 9.6% of the chloroplast genome was analysed, using six universal primer pairs and two restriction enzymes. All the mutations detected were insertion-deletions, ranging between 5–30 bp. The combination of all the mutations resulted in three haplotypes (H1, H2 and H3) in the 96 sweet cherry cultivars and a single haplotype (H4) in the five cultivars of sour cherry. The cpDNA polymorphism determined by PCR-RFLP markers helped to understand the maternal inheritance of chloroplast genome in sweet cherry, clearly distinguished the sour from the sweet cherry, and supported that P. avium is not the maternal species of P. cerasus. 相似文献
20.
Biochemical characterization of amandin,the major storage protein in almond (Prunus dulcis L.) 总被引:1,自引:0,他引:1
Sathe SK Wolf WJ Roux KH Teuber SS Venkatachalam M Sze-Tao KW 《Journal of agricultural and food chemistry》2002,50(15):4333-4341
The almond major storage protein, amandin, was prepared by column chromatography (amandin-1), cryoprecipitation (amandin-2), and isoelectric precipitation (amandin-3) methods. Amandin is a legumin type protein characterized by a sedimentation value of 14S. Amandin is composed of two major types of polypeptides with estimated molecular weights of 42-46 and 20-22 kDa linked via disulfide bonds. Several additional minor polypeptides were also present in amandin. Amandin is a storage protein with an estimated molecular weight of 427,300 +/- 47,600 Da (n = 7) and a Stokes radius of 65.88 +/- 3.21 A (n = 7). Amandin is not a glycoprotein. Amandin-1, amandin-2, and amandin-3 are antigenically related and have similar biochemical properties. Amandin-3 is more negatively charged than either amandin-1 or amandin-2. Methionine is the first essential limiting amino acid in amandin followed by lysine and threonine. 相似文献