Possibility of amino acid nutrition in broiler

The consumption of poultry meats exceeds 50% of total meat consumption. With the outbreak of bovine spongiform encephalopathy and foot and mouth disease, the consumption of poultry meat is set to increase. The use of meat bone meal derived from cattle and pigs is prohibited. Therefore, plant protein has to be used more frequently and the importance of the study of amino acid nutrition is increasing. In order to improve dietary efficiency, it is necessary to elucidate the exact amino acid requirements of poultry. The amino acid requirements are not constant and are affected by various factors. By using the plasma amino acid concentration as a parameter, it is possible to predict the amino acid requirements within a short period using a small number of animals repeatedly. By satisfying the amino acid requirements through supplementing crystalline amino acids, it is possible to reduce dietary protein and excretory nitrogen simultaneously. However, when the dietary protein is reduced, the abdominal fat tends to increase. This is solved by deciding the optimum dietary protein level, but it is difficult to control the taste of poultry meat by dietary amino acids.     

蛋鸡氨基酸营养的研究进展

近年来,随着畜牧业的发展,饲料原料匮乏以及环境污染问题日趋严重,作为蛋鸡营养中的重要内容,氨基酸的营养研究重点也在随之发生转变.以理想氨基酸模式及相应的分析研究方法为核心,在蛋鸡氨基酸需要量和氨基酸营养调控领域出现了大量新的研究成果与最新发现,从可持续发展、生态环保、动物福利等宏观方面到氨基酸的营养作用原理等机制研究上均有明显进展,研究不断深入.本文综述了近几年来产蛋鸡理想蛋白质模式、产蛋鸡和蛋雏鸡氨基酸需要量以及氨基酸营养调控方面的最新研究进展,并探讨了蛋鸡氨基酸营养在未来发展的可能趋势.     

鲜茶叶粉对蛋鸡生产性能、蛋品质、蛋黄脂肪酸水平及经济指标的影响

文章旨在评估日粮添加不同水平的鲜茶叶粉对蛋鸡生产性能、蛋品质及蛋黄脂肪酸水平的影响.试验将672只产蛋性能一致的28周龄海兰褐壳蛋鸡随机分为4组,每组4个重复,每个重复42只.对照组饲喂基础日粮,处理组蛋鸡分别饲喂基础日粮+20(T1)、40(T2)和60?mg/kg(T3)鲜茶叶粉,试验为期6周.结果:与对照组相比,...     

理想氨基酸模式下低粗蛋白质饲粮对蛋鸡生产性能的影响

本试验旨在研究以理想氨基酸(AA)模式配制的低粗蛋白质(CP)饲粮对21～32周龄蛋鸡生产性能、蛋品质、全血氨浓度和血浆尿酸浓度及粪氮含量的影响.试验选用540只体重相近的健康海兰灰蛋鸡,随机分为5组,每组6个重复,每个重复18只鸡,分别饲喂等能量( 11.00 MJ/kg)的5种CP水平(17.00％、16.50％、16.00％、15.50％和15.00％)的饲粮,试验期12周.结果表明:在理想AA模式下降低饲粮CP水平,蛋鸡产蛋率显著提高(P＜0.05),平均蛋重有降低的趋势(P=0.06),但对平均日采食量、日产蛋重和蛋料比无显著影响(P＞0.05);降低饲粮CP水平极显著影响了蛋白高度和哈氏单位(_P＜0.01),显著影响了蛋黄颜色(P＜0.05),未显著影响蛋壳厚度和蛋壳强度(P＞0.05);饲粮CP水平对各鸡蛋成分均无显著影响(P＞0.05),但鸡蛋蛋白含量随饲粮CP水平的降低有降低的趋势(P=0.09);饲粮CP水平对血浆尿酸浓度及全血氨浓度均无显著影响(P＞0.05);降低饲粮CP水平极显著降低了蛋鸡粪氮含量(P＜0.01).由此可见,在理想AA模式下,适当降低饲粮CP水平可提高蛋鸡产蛋率,改善鸡蛋品质,降低粪氮含量.     

低豆粕日粮对产蛋鸡生产性能及蛋品质的影响

试验选用1 600只生产性能相同、体况良好的33周龄罗曼蛋种鸡,随机分为4组,在总蛋白质含量相同和满足必需氨基酸需要量的情况下,用价格较低的蛋白饲料取代蛋鸡日粮中豆粕,研究氨基酸平衡的低豆粕日粮对蛋种鸡生产性能和蛋品质的影响。结果表明:氨基酸平衡的低豆粕日粮对蛋鸡产蛋率、种蛋合格率、健雏率、蛋形指数、蛋比重、蛋壳厚度、哈氏单位等指标无显著影响(P>0.05),但当日粮中的豆粕含量降低8～15个百分点时,蛋种鸡的日平均蛋重、日采食量、受精率、孵化率等显著低于正常豆粕日粮(P<0.05),而考虑综合因素日粮中的豆粕含量降低8个百分点的第1组,与正常豆粕日粮相比,差异不显著(P>0.05),而且能降低饲料成本,可以提高蛋鸡养殖效益,具有实际意义。     

乳酸锌对蛋鸡生产性能及蛋壳质量的影响

为研究乳酸锌对蛋鸡生产性能及蛋壳质量的影响,试验采用单因子随机分组试验设计,选取1600只360日龄的罗曼粉壳蛋鸡,随机分为4个处理组,每个处理组8个重复,每个重复50只鸡。础饲日粮为玉米-豆粕型粉状饲粮,处理1组饲喂基础饲粮+60 mg/kg一水硫酸锌(以锌计),处理2组饲喂基础饲粮+60 mg/kg乳酸锌(以锌计),处理3组饲喂基础饲粮+40 mg/kg乳酸锌(以锌计),处理4组饲喂基础饲粮+60 mg/kg锌(一水硫酸锌和乳酸锌提供的锌各一半)。结果表明,蛋鸡日粮中添加60 mg/kg乳酸锌(以锌计)能显著降低平均破蛋率、提高平均蛋壳强度(P<0.05)和明显提高平均蛋壳厚度,并且平均死亡率最低,但对蛋鸡的平均产蛋率、平均蛋重、平均日采食量和平均料蛋比无显著影响(P>0.05)。     

日粮中添加女贞子粉对蛋鸡生产性能和蛋品质的影响

选择150只49周龄、平均体重(1.99±0.17)kg的健康海兰褐蛋鸡,采用单因素完全随机分组试验设计,随机分为对照组(基础日粮)、试验组Ⅰ(基础日粮+0.5%女贞子粉)、试验组Ⅱ(基础日粮+1%女贞子粉)。每组设5个重复,每个重复10只蛋鸡,试验期为8周,研究了女贞子粉对蛋鸡生产性能和蛋品质的影响。结果表明:与对照组相比,试验组Ⅰ、Ⅱ产蛋率分别提高了4.86%和11.75%(P0.05)。试验组Ⅰ蛋黄颜色显著高于对照组9.60%(P0.05)。日粮中添加女贞子粉可以提高蛋鸡产蛋率,改善蛋黄颜色,进而提高蛋鸡的生产性能和蛋品质。     

乳酸锌对蛋鸡生产性能及蛋壳质量的影响

本试验旨在研究乳酸锌对蛋鸡生产性能及蛋壳质量的影响。试验采用单因子随机分组试验设计,选取1600只360日龄的罗曼粉壳蛋鸡,随机分到4个处理组,各处理组8个重复,每个重复50只鸡。基础饲粮采用为玉米－豆粕型粉状饲粮,处理1饲喂基础饲粮＋60mg／kg－水硫酸锌（以锌计）,处理2饲喂基础饲粮＋60mg／kg乳酸锌（以锌计）,处理3饲喂基础饲粮＋40mg／kg乳酸锌（以锌计）,处理4饲喂基础饲粮＋60mg／kg锌（－水硫酸锌和乳酸锌提供的锌各一半）。试验结果表明,在产蛋鸡饲粮中100％以乳酸锌替代硫酸锌（提供的锌含量为60mg／kg）,能显著降低平均破蛋率,提高平均蛋壳强度和平均蛋壳厚度（P〈0．05）,死亡率最低,对蛋鸡的平均产蛋率、平均蛋重、平均日采食量和平均料蛋比无显著影响（P〉0．05）。     

Long‐term feeding effects of dietary protein levels on egg production,immunocompetence and plasma amino acids of laying hens in subtropical condition

The objective of this study was to determine the effect of protein levels on production performance, immune response, liver triglyceride and plasma amino acids profile of laying hens during 21–48 weeks of age. Two groups of commercial hens (Babcock B‐308) were fed with diets with different crude protein levels (14% and 18% CP). The high protein diet (18% CP) significantly increased production performance, essential amino acid intake, spleen weight (% of body weight), ND‐titre (haemagglutination‐inhibition test), serum albumin (g/dl), serum alpha‐globulin and plasma essential amino acids (except histidine) and decreased liver triglyceride (mg/g liver) in relation to the low protein diet (14% CP). In long‐term effect, therefore, this study suggested that adequate dietary condition of amino acids, particularly methionine and branch‐chain amino acids is necessary for sustaining normal immunocompetence and achieving maximum production performance.     

Effects of Antimicrobial peptides on egg production,egg quality and caecal microbiota of hens during the late laying period

This study was conducted to determine the effects of diet supplementation of laying hens with antimicrobial peptides (AMP) on egg production, egg quality and caecal microbiota. A total of 360 Hy‐Line Brown laying hens (72 weeks old) were divided into three groups with four replicates of 30 birds each. The laying hens were fed with the basal diet (Control), the basal diet + 50 mg/kg AMP (group 1) and the basal diet + 100 mg/kg AMP (group 2). The experiment lasted for 45 d. Eggs were collected daily and caecal samples were collected at the end of the experiment. The results showed that AMP supplementation caused a significantly increased laying rate and decreased feed/egg ratio (p ＜ .05). Meanwhile, a distinctive difference in cecal microbiota was observed between AMP and control groups and the average values of microbial diversity and richness were lower in the AMP group than in the control group. At the phylum level, the relative abundance of Verrucomicrobia and Cyanobacteria were lower in the AMP group than in the control group. In conclusion, the results indicated that dietary supplementation with AMP can improve egg production and affect the cecal microbial community membership and structure of hens during late laying period.     

蛋鸡产蛋性能和蛋壳品质耐受低水平磷的应用研究

本研究选择健康49周龄褐壳蛋鸡1296只,随机分为6组,每组2个重复,每个重复108只。A为对照组,含0.20%有效磷(AP)和0.9‰植酸酶;B、C、D、E、F组分别含AP0.16%、0.12%、0.20%、0.16%和0.12%,其中B、C组添加0.9‰植酸酶。试验期8周。结果显示,试验4、6、8周和试验全期,F组比对照组产蛋率分别降低6.67%、7.78%、7.87%和6.67%,差异均显著(P0.05);各组平均蛋重和采食量无显著差异;试验6、8周和试验全期,F组料蛋比较对照组分别高10.19%、11.30%和8.00%,差异均显著(P0.05)。8周试验全期,C、D、E、F组软破蛋率和各组斑蛋率均高于对照组(P0.05);F组出现9只瘫鸡,其他各组均没有出现。提示日粮AP降至0.12%时,对蛋鸡产蛋性能、蛋壳品质自第3～4周开始产生影响。     

The effects of formic acid-treated shrimp meal on laying performance and egg quality in aged laying hens

An experiment was carried out to measure the suitability of using formic acid-treated shrimp meal (TSM) as a protein source for aged laying hens. Shrimp meal (SM), air-dried shrimp waste was treated with 3% formic acid (w/v) at room temperature for 20 min, sun-dried, ground to pass through 1.0 mm mesh screen and then ready to use as the TSM. A total of 56 Single Comb White Leghorn hens, 85 wk of age, were placed in individual cages and assigned randomly into 7 dietary groups of 8 birds each. A control diet, diets containing 5, 10, and 15% SM and diets containing 5, 10, and 15% TSM were prepared. Diets and water were given ad libitum during the 30-d experimental period. Hen-day egg production, egg mass, feed intake, and feed conversion ratio (FCR) were deteriorated with increasing levels of the SM in the diets (P < 0.05). In contrast, these parameters were unchanged with increasing levels of the TSM in diets and compatible with the control group. No significant improvements were observed in exterior or interior egg quality among the dietary groups, except for eggshell weight, strength and yolk color, which were significantly increased in SM and TSM groups when compared to the values in control group (P < 0.05). It is concluded that inclusion of TSM up to 15% in laying hen diets may have beneficial effects on laying performance along with improved eggshell quality and yolk color, and suggested that TSM is a promising combined source of protein and calcium in aged laying hens.     

标准回肠可消化氨基酸模式下降低饲粮粗蛋白质水平对蛋鸡生产性能、蛋品质及氮平衡的影响

本试验旨在研究标准回肠可消化(SID)氨基酸(AA)模式下,降低饲粮粗蛋白质(CP)水平对海兰灰蛋鸡生产性能、蛋品质及氮平衡的影响。选取540只20周龄体重相近且健康的海兰灰蛋鸡,随机分为5个处理,每个处理6个重复,每个重复18只鸡,预试期1周,正试期12周,采食等代谢能(11.82 MJ/kg)、等赖氨酸(Lys,SID Lys为0.831%)、相同AA模式、不同CP水平(18.0%、17.5%、17.0%、16.5%和16.0%)的试验饲粮。结果表明:SID AA模式下,降低饲粮CP水平可极显著降低平均蛋重和CP摄入量(P<0.01),显著降低鸡蛋浓蛋白高度(P<0.05),显著降低蛋鸡摄入氮和排出氮(P<0.05),有提高氮表观利用率的趋势(P=0.05);数据偏相关分析表明,饲粮中SID AA模式外的精氨酸、苯丙氨酸和亮氨酸水平下降与平均蛋重下降显著相关(P<0.05),与浓蛋白高度下降无显著相关(P>0.05)。结果提示,SID AA模式下,降低饲粮CP水平,除影响平均蛋重和浓蛋白高度外,对其他生产性能、蛋品质及血液生化指标影响较小,平均蛋重的下降与低CP饲粮中精氨酸、苯丙氨酸和亮氨酸不足有关;AA平衡的低CP饲粮,可在维持较高生产性能的同时减轻蛋鸡粪氮排出。     

不同锰源与水平对蛋鸡产蛋后期生产性能和蛋品质的影响

试验旨在研究添加不同锰源与水平羟基蛋氨酸锰对蛋鸡产蛋后期生产性能和蛋品质的影响。对照组饲喂加含106 mg/kg硫酸锰(以锰计)的基础日粮,其余各组分别添加30、50、70 mg/kg的羟基蛋氨酸锰(以锰计)来替代基础日粮中的硫酸锰。结果表明:与对照组相比,试验第14 d,日粮中添加30 mg/kg羟基蛋氨酸锰显著提高了蛋壳厚度(P<0.05),添加50 mg/kg羟基蛋氨酸锰显著提高了蛋壳重(P<0.05);试验第28 d,日粮中添加50 mg/kg羟基蛋氨酸锰显著提高了蛋壳厚度(P<0.05),添加30 mg/kg羟基蛋氨酸锰显著改善蛋壳颜色(P<0.05);试验第42 d,蛋壳厚度与添加量呈二次曲线关系,蛋壳厚度在添加量为50 mg/kg时达到最大值(P<0.01)。与对照组相比,试验第14 d,日粮中添加30 mg/kg羟基蛋氨酸锰显著降低了鸡蛋的哈夫单位和蛋黄比例(P<0.05)。试验第42 d,与对照组相比,日粮中添加30 mg/kg羟基蛋氨酸锰显著降低了鸡蛋的蛋黄比例(P<0.05)。试验结果表明,添加50 mg/kg的羟基蛋氨酸...     

Effect of expanded cottonseed meal on laying performance,egg quality,concentrations of free gossypol in tissue,serum and egg of laying hens

Three hundred and sixty Hy‐Line Brown hens, 40 week of age, were allocated to five treatments, each of which included four replicates of 18 hens. After an expanded process of cottonseed meal (CSM), free gossypol content in CSM was decreased from 1.24 to 0.40 g/kg. The dietary treatments were corn‐soybean meal based diets including 6% CSM and 6%, 8% and 10% expanded cottonseed meal (ECSM). Hens fed 8% ECSM had higher (P < 0.05) laying rate and average egg weight than those fed 6% CSM. The albumen height and Haugh unit in the control group, 6% and 8% ECSM groups were superior (P < 0.05) to other treatments. Hens fed 6% CSM resulted in severe (P < 0.05) egg yolk discoloration. Free gossypol (FG) concentrations in yolk and albumen and tissues of the 6% CSM group were greater (P < 0.05) than those in any ECSM treatments. Hens fed 6% CSM and 10% ECSM had the highest (P < 0.05) FG concentrations in the liver compared with those in the kidney and muscle, and higher (P < 0.05) FG residues in yolk than those in albumen. In conclusion, FG in CSM can be reduced by 68% through an expanded process and ECSM can be available in laying hens at up to 10% of the total diet and an appropriate replacement of soybean meal with ECSM may improve performance in laying hens.     

茶皂素对蛋鸡生产性能、蛋品质和养分利用率的影响

试验旨在研究饲粮中添加不同水平茶皂素对蛋鸡生产性能、蛋品质和养分利用率的影响.选取600只40周龄健康的海兰褐商品蛋鸡,随机分为4组,每组6个重复,每个重复25只鸡.对照组饲喂基础饲粮,试验组在基础饲粮中分别添加100、200、300 mg/kg的茶皂素.预试期7d,正式试验期56 d.结果表明:与对照组相比,100、...     

Genome‐wide association study for egg production and quality in layer chickens

Discovery of genes with large effects on economically important traits has for many years been of interest to breeders. The development of SNP panels which cover the whole genome with high density and, more importantly, that can be genotyped on large numbers of individuals at relatively low cost, has opened new opportunities for genome‐wide association studies (GWAS). The objective of this study was to find genomic regions associated with egg production and quality traits in layers using analysis methods developed for the purpose of whole genome prediction. Genotypes on over 4500 birds and phenotypes on over 13 000 hens from eight generations of a brown egg layer line were used. Birds were genotyped with a custom 42K Illumina SNP chip. Recorded traits included two egg production and 11 egg quality traits (puncture score, albumen height, yolk weight and shell colour) at early and late stages of production, as well as body weight and age at first egg. Egg weight was previously analysed by Wolc et al. ( 2012 ). The Bayesian whole genome prediction model – BayesB (Meuwissen et al. 2001 ) was used to locate 1 Mb regions that were most strongly associated with each trait. The posterior probability of a 1 Mb window contributing to genetic variation was used as the criterion for suggesting the presence of a quantitative trait locus (QTL) in that window. Depending upon the trait, from 1 to 7 significant (posterior probability >0.9) 1 Mb regions were found. The largest QTL, a region explaining 32% of genetic variance, was found on chr4 at 78 Mb for body weight but had pleiotropic effects on other traits. For the other traits, the largest effects were much smaller, explaining <7% of genetic variance, with regions on chromosomes 2, 12 and 17 explaining above 5% of genetic variance for albumen height, shell colour and egg production, respectively. In total, 45 of 1043 1 Mb windows were estimated to have a non‐zero effect with posterior probability > 0.9 for one or more traits.