Antifeedant and sublethal effects of imidacloprid on Asian citrus psyllid,Diaphorina citri

BACKGROUND: Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, transmits the causal bacteria of the devastating citrus disease huanglongbing (HLB). Because of the variation in spatial and temporal uptake and systemic distribution of imidacloprid applied to citrus trees and its degradation over time in citrus trees, ACP adults and nymphs are exposed to concentrations that may not cause immediate mortality but rather sublethal effects. The objective of this laboratory study was to determine the effects of sublethal concentrations of imidacloprid on ACP life stages. RESULTS: Feeding by ACP adults and nymphs on plants treated daily with a sublethal concentration (0.1 µg mL^?1) of imidacloprid significantly decreased adult longevity (8 days), fecundity (33%) and fertility (6%), as well as nymph survival (12%) and developmental rate compared with untreated controls. The magnitude of these negative effects was directly related to exposure duration and concentration. Furthermore, ACP adults that fed on citrus leaves treated systemically with lethal and sublethal concentrations of imidacloprid excreted significantly less honeydew (7–94%) compared with controls in a concentration‐dependent manner suggesting antifeedant activity of imidacloprid. CONCLUSIONS: Sublethal concentrations of imidacloprid negatively affect development, reproduction, survival and longevity of ACP, which likely contributes to population reductions over time. Also, reduced feeding by ACP adults on plants treated with sublethal concentrations of imidacloprid may potentially decrease the capacity of ACP to successfully acquire and transmit the HLB causal pathogen. Copyright © 2009 Society of Chemical Industry     

Effect of pyriproxyfen,a juvenile hormone mimic,on egg hatch,nymph development,adult emergence and reproduction of the Asian citrus psyllid,Diaphorina citri Kuwayama

BACKGROUND: The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is a vector of bacteria presumably responsible for huanglongbing (HLB) disease in citrus. In this laboratory study, an investigation was made of the activity of pyriproxyfen, a juvenile hormone mimic, on ACP eggs, nymphs and adults to evaluate its potential as a biorational insecticide for inclusion in an integrated pest management (IPM) program for ACP. RESULTS: Irrespective of egg age, timing or method of treatment, a significantly lower percentage of eggs (5–29%) hatched after exposure to 64 and 128 µg mL^?1 of pyriproxyfen. Only 0–36% of early instars (first, second and third) and 25–74% of late instars (fourth and fifth) survived to adults following exposure to 16, 32 and 64 µg mL^?1 of pyriproxyfen. However, 15–20% of adults that emerged following treatment as late instars exhibited morphological abnormalities. Furthermore, pyriproxyfen adversely affected reproduction (fecundity and fertility) of adults that emerged from treated fifth instars or that were treated topically with 0.04 µg as adults. CONCLUSIONS: Application of pyriproxyfen at 64 µg mL^?1 resulted in greater inhibition of egg hatch and suppression of adult emergence compared with lower rates. Pyriproxyfen also markedly reduced female fecundity and egg viability for adults that were exposed either as fifth instars or as newly emerged adults. The ovicidal, larvicidal and reproductive effects against ACP suggest that pyriproxyfen is suitable for integration into an IPM program for ACP. Copyright © 2009 Society of Chemical Industry     

Effects of pymetrozine, an antifeedant of Hemiptera, on Asian citrus psyllid, Diaphorina citri, feeding behavior, survival and transmission of Candidatus Liberibacter asiaticus

BACKGROUND: Vector‐borne plant disease management can be enhanced by deployment of antifeedants in addition to the use of broad‐spectrum neurotoxic insecticides. The effects of pymetrozine on Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, feeding behaviour, survival and transmission of Candidatus Liberibacter asiaticus (Las), the presumed causal pathogen of huanglongbing, were investigated. RESULTS: Pymetrozine applied at 52 and 104 µg mL^?1 to citrus plants [Swingle citrumelo (X Citroncirus webberi Ingram and Moore)] modified the feeding behavior of ACP and increased the amount of time spent performing non‐penetration behaviors while decreasing the time spent performing ingestion behaviors compared with the controls 1 day after treatment. However, the antifeedant effect of pymetrozine subsided 5 days after application. Pymetrozine reduced the survival of both adults and nymphs on treated plants compared with the control. However, it had a greater impact on survival of nymphs than on survival of adults. Pymetrozine applied at 52 and 104 µg mL^?1 on Las‐infected ‘Valencia’ sweet orange plants [Citrus sinensis L. (Osbeck)] reduced acquisition (12 and 21% respectively) and transmission (11 and 18% respectively) of Las by feeding ACP adults compared with the controls; however, these reductions were not statistically significant. CONCLUSIONS: Pymetrozine exhibited moderate antifeedant effects by modifying the feeding behavior of ACP adults with short residual activity. The impact of pymetrozine on survival of nymphs was greater than on adults at the higher concentrations tested. Pymetrozine also reduced the acquisition and transmission of Las by feeding ACP adults up to 21 and 18%, respectively, compared with untreated controls. Copyright © 2010 Society of Chemical Industry     

Quantification and ecological study of ‘Candidatus Liberibacter asiaticus’ in citrus hosts,rootstocks and the Asian citrus psyllid

The use of proper management strategies for citrus huanglongbing (HLB), caused by ‘Candidatus Liberibacter asiaticus’ (Las) and transmitted by Asian citrus psyllid (ACP) (Diaphorina citri), is a priority issue. HLB control is based on healthy seedlings, tolerant rootstock cultivars and reduction of ACP populations. Here, dynamic populations of Las in different citrus hosts and each instar of ACP were studied, together with the seasonal growth and distribution of Las in different tissues, using conventional and TaqMan real‐time PCR. Different levels of susceptibility/tolerance to HLB were seen, resulting in different degrees of symptom severity and growth effects on hosts or rootstocks. Troyer citrange, Swingle citrumelo and wood apple were highly tolerant among 11 rootstock cultivars. Regarding distribution and seasonal analysis of Las, mature and old leaves contained high concentrations in cool temperatures in autumn and spring. Las was detected earlier through psyllid transmission than through graft inoculation, and the amounts of Las (AOL) varied in different hosts. Thus, different AOL (10⁴–10⁷ copy numbers μL^?1) and Las‐carrying percentages (LCP; 40–53.3%) were observed in each citrus cultivar and on psyllids, respectively. Furthermore, both AOL and LCP were lower in nymphs than in adult psyllids, whereas the LCP of psyllids were not affected by increasing the acquisition‐access time. The present study has significant implications for disease ecology. The combination of early detection, use of suitable rootstocks and constraint of psyllid populations could achieve better management of HLB disease.     

Tolerance of spring wheat (Triticum aestivum L.) to trifluralin deep-incorporated in the autumn or spring

Field experiments at Lacombe on a Ponoka loam soil (9·6% organic matter) during 1982 and 1983 investigated the tolerance of spring wheat (Triticum aestivum L.) cv. Neepawa in a weed free situation to trifluralin applied at 0·0–3·0 kg ai ha^?1 in the autumn or spring and incorporated to a depth of 10 cm. Rates of trifluralin above 1·0 kg ai ha^?1 applied in the autumn or spring reduced the percent stand of wheat compared to an untreated control. Two weeks after emergence the crop showed 37 or 47% injury indicated by delayed growth, following application at 1·0 kg ai ha^?1 in the autumn or spring, respectively. The wheat recovered throughout the course of the growing season. At harvest, trifluralin applied in the autumn or spring at rates below 1·0 kg ai ha^?1 caused a yield increase while higher rates caused a yield decrease compared to the untreated control. Spring application caused a greater yield loss than autumn application. The tolerance of spring wheat to trifluralin at rates required for weed control (1·1 kg ai ha^?1 or higher) on this soil type is marginal.     

Effect of Candidatus Liberibacter asiaticus infection on susceptibility of Asian citrus psyllid, Diaphorina citri, to selected insecticides

BACKGROUND: In the present investigation, the effect of Candidatus Liberibacter asiaticus (Las), a bacterium considered to be responsible for causing huanglongbing (HLB) disease in citrus, on the physiology of its vector, the Asian citrus psyllid (ACP) Diaphorina citri Kuwayama, was determined. Specifically, the effects of Las infection on the susceptibility of ACP to selected insecticides were determined. Furthermore, total protein content and general esterase activity were quantified in Las‐infected and uninfected ACP to gain insight into the possible mechanism(s) responsible for altered susceptibility to insecticides owing to Las infection. RESULTS: LC₅₀ values were significantly lower in Las‐infected than in uninfected ACP adults for chlorpyrifos and spinetoram. Furthermore, there was a general trend towards lower LC₅₀ values for three other insecticides for Las‐infected ACP; however, the differences were not statistically significant. Total protein content (µg mL^?1) was significantly lower in Las‐infected (23.5 ± 1.3 in head + thorax; 27.7 ± 1.9 in abdomen) than in uninfected (29.7 ± 2.1 in head + thorax; 35.0 ± 2.3 in abdomen) ACP. Likewise, general esterase enzyme activity (nmol min^?1 mg^?1 protein) was significantly lower in Las‐infected (111.6 ± 4.5 in head + thorax; 109.5 ± 3.7 in abdomen) than in uninfected (135.9 ± 7.5 in head + thorax; 206.1 ± 23.7 in abdomen) ACP. CONCLUSION: Susceptibility of ACP to selected insecticides from five major chemistries was greater in Las‐infected than in uninfected ACP. The lower total protein content and reduced general esterase activity in Las‐infected than in uninfected ACP may partly explain the observed higher insecticide susceptibility of Las‐infected ACP. Copyright © 2010 Society of Chemical Industry     

Chemical weed control in transplanted onion (Allium cepa L.) in the Sudan Gezira

Unrestricted weed growth reduced the yield of transplanted onions (Allium cepa L.) by 26–48%. Hand-weeding once, 42 days after transplanting reduced the yield loss to 15%. Application of oxadiazon (1.1–1.3 kg ai ha^?1), pendimethalin (1.2–1.8 kg ai ha^?1), oxyfluorfen (0.14–0.43 kg ai ha^?1) and chlorthal-dimethyl (8.3–13.1 kg ai ha^?1) resulted in yields comparable to the hand-weeded control (82–126%). When sprayed at transplanting these herbicides gave excellent and persistent control of grasses (86–100%). However, they were less effective on broad-leaved weeds (38–94% control). Désherbage chimique de l'oignon repiqué (Allium cepa L.) dans la Gezireh soudanaise La croissance incontrôlée des mauvaises herbes réduit le rendement des oignons repiqués de 26–48%. Un désherbage manuel, 42 jours après le repiquage, ramène la baisse de rendement à 15%. L'application d'oxadiazon (1,1–1,3 kg ma ha^?1), de pendiméthalin (1,2–1,8 ka ma ha¹), d'oxyfluorfen (0,14–0,43 ka ma ha^?1) et de chlortal-diméthyle (8,3–13,1 kg ma ha^?1), a permis d'atteindre des rendements comparables aux témoins désherbés manuellement (82–126%). Lorsqu'ils ont été administrés au moment du repiquage, ces herbicides ont permis un contrôle efficace et persistant des monocotylédones (86–100%). Toutefois, ils se sont montrés moins efficaces à l'égard des dicotylédones (38–94% du témoin). Chemische Unkrautbekämpfung in verpflanzten Zwiebeln (Allium cepa L.) in der Region Gezira (Sudan) Ungehinderte Unkrautentwicklung in verpflanzten Zwiebeln (Allium cepa L.) verringerte die Ernte um 26–48%. Eine Handjätung, 42 Tage nach dem Auspflanzen, reduzierte den Ernteverlust auf 15%. Die Applikation von Oxadiazon (1,1–1,3 kg ai ha^?1), Pendimethalin (1,2–1,8 kg ai ha^?1), Oxyfluorfen (0,14–0,43 kg ai ha^?1) und Chlorthal- dimethyl (8,3–13,1 kg ai ha^?1) erbrachte Ernten, die mit denjenigen, der von Hand gejäteten Kontrollparzellen vergleichbar waren (82–126%). Diese Herbizide, zur Ausflanzzeit ausgebracht, ergaben eine ausgezeichnete und andauernde Kontrolle von Gräsern (86–100%), während die Wirkung gegen breitblättrige Unkräuter geringer ausfiel (38–94%).     

Lethal and sub-lethal effects of a novel sulfoximine insecticide,sulfoxaflor, against Asian citrus psyllid and its primary parasitoid under laboratory and field conditions

The Asian citrus psyllid, Diaphorina citri Kuwayama, is the most important international pest of citrus because it transmits the bacteria that cause huanglongbing (HLB). HLB limits citrus production globally. We evaluated the toxicity of sulfoxalor against D. citri and its parasitoid, Tamarixia radiata Waterston. Sulfoxaflor was as toxic as imidacloprid to adult D. citri. The LC₅₀ values for sulfoxaflor and imidacloprid were 8.17 and 5.7 µg AI mL^?1, respectively. The LC₅₀ of sulfoxaflor for T. radiata adults was 3.3 times greater than for D. citri adults. Treatment with sulfoxaflor resulted in reduced oviposition, development of nymphs, and emergence of adult D. citri on plants, as compared with controls. The lowest concentration that reduced adult emergence was 0.6 µg AI mL^?1. There was reduced feeding by D. citri adults on leaves treated with sulfoxaflor. The residual toxicity of sulfoxaflor was equivalent to imidacloprid. Under field conditions, formulated sulfoxaflor reduced populations of D. citri compared with untreated controls. Sulfoxaflor is a novel mode of action and is an effective tool for D. citri management.     

Cereal leaf beetles (Oulema spp., Coleoptera: Chrysomelidae) control following various dates of wheat sowing and insecticidal treatments

In this paper, we report the sowing date and intensity of chemical control on presence of the cereal leaf beetles (CLB) on spring wheat. The CLB monitoring (Oulema melanopus L. and Oulema gallaeciana Voet.) was conducted in May–June of each year, following the announcement of the IOR-PiB in Poznań (Institute of Plant Protection, National Research Institute), that economic threshold levels in Poland of this pest is observed. For the spring sowing dates between (20 March–20 April) and late autumn sowings (21 November–7 December) larger amounts of CLB adults (1.6–1.1) in comparison to early autumn sowings (25 October–02 November) were noticed (0.4). Following the insecticidal seed treatment with imidacloprid, the occurrence of adults was 0.2–0.7 individual per stem, while the use of fungicidal treatment with triadimenol–imazalil–fuberidazol resulted in average 1.1–2.2 individual per stem. The reduction in the number of larvae per stem was 7–10 fold larger following the foliar application (pirimicarb + dimethoate + cypermethrin) compared to the seed treatment with imidacloprid. The lowest grain yield (3.83 t ha^?1) from the spring crop without any insecticide was obtained. Foliar application with blend of pirimicarb + dimethoate + cypermethrin increased the yield crop by 0.41 t ha^?1 (10.7%) and additionally by 0.78 t ha^?1 (20.4%) to compare to the fungicidal control when imidacloprid was used. Wheat seed treatment with imidacloprid and sowing date in autumn showed to be suitable and economically efficient preventive strategies for controlling the pest population.     

Effects of repeated applications of fluazifop-butyl, haloxyfop and sethoxydim on Elymus repens in strawberries

Fluazifop-butyl, haloxyfop and sethoxydim with adjuvant oil were applied for three successive growing seasons to established strawberries infested with Elymus repens. The weed was virtually eradicated by three applications of haloxyfop (0.8 kg a.e. ha^?1) in successive years. A similar effect was given by five applications of haloxyfop (0.4 kg a.e. ha^?1) and fluazifop-butyl (1.6 kg AI ha^?1) in three growing seasons. Lower rates of haloxyfop and fluazifop-butyl, and a high rate of sethoxydim (1.6 kg AI ha^?1) applied five times were less effective, the ground cover of E. repens shoots not being reduced by the end of the experiment although weight of living rhizome was only 10-30% of that on untreated plots. Fruit yields on treated plots generally reflected the level of E. repens control, but there was some evidence of crop phytotoxicity from herbicide treatments. During the experiment E. repens untreated plots increased from about 30-100% ground cover and reduced yield 13, 28 and 68% in successive years compared with weed-free plots.     

The push–pull strategy for citrus psyllid control

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the only natural vector of Candidatus Liberibacter asiaticus that causes citrus huanglongbing (HLB), a most destructive disease of citrus. Currently, no remedial therapy exists for the disease, and so effective control of ACP is very important in curbing the transmission of the disease. The push–pull strategy should be thoroughly explored as an approach to ACP management. This mini‐review summarises the current progress towards more effective repellent and attractant chemicals through investigating known repellent and attractive plants. Interactions between ACP and its host plants are also addressed, with emphasis on the possible involvement of the host biochemicals in attracting the insect. Potential ways to increase the effectiveness of the pull–push strategy are briefly discussed. It is expected that the pull–push strategy will be gradually developed following more extensive research. © 2014 Society of Chemical Industry     

Selective herbicides for establishing weed-free grass: The effect of mixtures and sequences of herbicides, including ethofumesate and methabenzthiazuron

Two field experiments compared various sequences of ethofumesate, methabenzthiazuron and mecoprop, and mixtures of metamitron, ethofumesate and methabenzthiazuron for their control of Poa annua L. and Stelluria media (L). Vill. during the establishment of autumn-sown perennial ryegrass (Lolium perenne L.). In experiment 1 pre-emergence treatments required a second, post-emergence spraying to give complete control of P. annua. Grass seedlings reappeared in early spring following ethofumesate treatment, but populations built up earlier where methabenzthiazuron had been used. The most successful treatment was ethofumesate at 1.4 kg a.i. ha^?1 followed by 0.7 kg a.i, ha^?1 in spring. Methabenzthiazuron at 0.8 kg a.i. ha^?1 followed by ethofumesate at 0.7 kg a.i. ha^?1 in December was also effective. Methabenzthiazuron at 1.6 kg a.i. ha^?1 damaged ryegrass but was effective as a secondary treatment at lower doses in spring. Most treatments controlled S. media satisfactorily, but this sometimes increased the yield of P. annua. Metamitron greatly reduced tiller numbers of P. annua in experiment 2, especially when sprayed with methabenzthiazuron. Only treatments which included ethofumesate controlled S. media adequately but there was no advantage in combining this herbicide with any other for controlling P. annua.     

Control of Achillea millefolium L. (yarrow) by rotary cultivation and glyphosate

Various control strategies for Achillea millefolium L. (yarrow) were investigated in a dense stand of the weed at Lincoln College in 1977–1978. In early spring plots were either rotary cultivated or left undisturbed. In late spring, plots of both previous treatments were either left undisturbed, rotary cultivated or sprayed with glyphosate at 1·5 kg ha^?1. The whole experiment was rotary cultivated twice 1 week later and sown with Hordeum vulgure L. cv. Zephyr (barley) at 144 kg seed ha^?1. MCPA + dicamba at 0·9+0·15 kg ha^?1 was applied to half of each plot when the second node was detectable (Zadok 32). Rotary cultivation and glyphosate both substantially reduced the regrowth of A. millefolium but glyphosate reduced regrowth by a greater proportion when applied to undisturbed plants than when applied to plants regenerating after cultivation. Both gave a more than 95% reduction compared to the control (rotary cultivation only at sowing time) in the amount of A. millefolium present in the barley stubble in the autumn. MCPA + dicamba caused seedling mortality but did not affect the numbers of primary shoots from rhizome fragments. The grain yield of the barley increased from 2·91 t ha^?1 when A. millefolium was not controlled to 4·23 t ha^?1 with good control. The barley yield appeared to be restricted by competition from regenerating A. millefolium and by a nitrogen deficiency induced in some regimes by nitrogen immobilization in decaying rhizomes.     

Investigations on Oecophylla longinoda (Latreille) (Hymenoptera: Formicidae) as a biocontrol agent in the protection of cashew plantations

BACKGROUND: Cashew (Anacardium occidentale L.) has become a very important non‐traditional tree crop in Ghana. The crop is, however, attacked by sap‐sucking insects, particularly the mosquito bug, Helopeltis schoutedeni Reuter, the leaf‐footed bug, Pseudotheraptus devastans (Dist.), and the coreid bug, Anoplocnemis curvipes (F.), which feed on shoots, panicles and fruits. Their damage is characterised by withering of the latter. In Ghana, Oecophylla longinoda Latr. occurs in large numbers on cashew and other native plants, but little is known about its relationship with insect pests. The relationship between O. longinoda and shoot and panicle damage by sap‐sucking bugs and the effectiveness of O. longinoda as a biocontrol agent in the protection of cashew as compared with two chemical insecticides, lambda‐cyhalothrin (Karate^®) and cypermethrin + dimethoate (Cyperdim^®), were therefore investigated at Bole in the northern region of Ghana. RESULTS: There was a negative correlation between numbers of O. longinoda nests and pest damage. Trees treated with cypermethrin + dimethoate (969 mg AI mL^?1 tree^?1) and lambda‐cyhalothrin (100 mg AI mL^?1 tree^?1) recorded the smallest bug numbers, followed by O. longinoda. Trees infested by Oecophylla longinoda and trees treated with cypermethrin + dimethoate and with lambda‐cyhalothrin had less than 6% pest damage to shoots, panicles and fruits, while water‐sprayed trees recorded damage as high as 36.8% (shoots) in February, 32.9% (panicles) in February and 37.8% (fruits) in March. Cypermethrin + dimethoate again recorded the highest (485.0 kg ha^?1) nut yield, followed by O. longinoda (431.0 kg ha^?1), with water recording the lowest (93.0 kg ha^?1) nut yield. CONCLUSION: The results indicate that O. longinoda can be used to control some sucking bugs as effectively as some insecticides. Copyright © 2008 Society of Chemical Industry     

Impact of Irrigation Timing and Weed Management Practices on Chlorophyll Content and Morphological Traits of Tomato (Solanum lycopersicum Mill.)

Weeds are a major biotic constraint; compete with crop for the same resources and ultimately reduce productivity. This study evaluated the impact of irrigation intervals and weed management treatments on chlorophyll content and morphological growth of tomato to find an appropriate integrated weed management strategy. Two-year field experiments (2018/2019) were conducted at district Mardan (34°15′38″ N and 72°6′36″ E). Tomato F1 hybrid (Taj?3592) was transplanted during March. The experiments were laid out in a randomized complete-block design in split-plot arrangement with three replications. The main block comprised three irrigation intervals (3, 6, and 9 days) and the sub-block included weed management treatments: transparent polythene, black polythene, weeding except Orobanche, sole weeding of Orobanche, weeding of all weeds, copper oxychloride 1.5?kg a.i ha^?1 (single dose), copper oxychloride 1.5?kg a.i ha^?1 (split doses), copper oxychloride?+?humic acid 25?kg ha^?1 (single dose), copper oxychloride?+?humic acid 25?kg ha^?1 (split doses), copper sulphate 2?kg ha^?1 (single dose), copper sulphate 2?kg ha^?1 (split doses), ammonium sulphate 200?kg ha^?1 (single dose), ammonium sulphate 200?kg ha^?1 (split doses), pendimethalin 33 EC 1.44?kg a.i ha^?1, glyphosate 48 SL 1.5?kg a.i ha^?1, and weedy check. Lowest relative weed density (RWD) of O. cernua (2.23%) and highest RWD of O. cernua (38.01%) were recorded in the 3? and 9?day irrigation intervals, respectively. However, 3?day irrigation interval resulted in highest fresh weed biomass (5794?kg ha^?1). Moreover, the 6?day irrigation interval significantly increased chlorophyll content by 11 and 5%, leaf area by 23 and 6%, and number of branches plant^?1 by 30 and 22% compared to 9? and 3?day irrigation intervals, respectively. Among the weed management treatments, black polythene resulted in the highest weed control efficiency (96%), increasing chlorophyll content by 16%, leaf area by 33%, and number of branches plant^?1 by 64% vs. weedy check. Consequently, 6?day irrigation intervals?×?black polythene could be the best weed management strategy, followed by transparent polythene, weeding of all weeds, pendimethalin, glyphosate, and ammonium sulphate.

     

Insecticide resistance in field populations of Asian citrus psyllid in Florida

BACKGROUND: Asian citrus psyllid (ACP), Diaphorina citri, is a major pest of citrus because it vectors the putative causal agent of huanglongbing disease. Insecticides are currently the basis of psyllid management programs, and the number of annual insecticide applications has increased significantly. In this paper, a series of investigations of insecticide resistance among field populations of adult and immature ACP in Florida is described. RESULTS: In 2009, the highest level of resistance for adult ACP, as compared with the laboratory susceptible (LS) population, was found with imidacloprid with an LD₅₀ resistance ratio (RR₅₀) of 35 in one population. This was followed by chlorpyriphos (RR₅₀ = 17.9, 13.3, 11.8 and 6.9), thiamethoxam (RR₅₀ = 15 and 13), malathion (RR₅₀ = 5.4 and 5.0) and fenpropathrin (RR₅₀ = 4.8). In 2010, mortality of adults from all five sites sampled was lower than with the LS population at three diagnostic concentrations of each insecticide tested. Among nymph populations, indications of resistance were observed with carbaryl (RR₅₀ = 2.9), chlorpyriphos (RR₅₀ = 3.2), imidacloprid (RR₅₀ = 2.3 and 3.9) and spinetoram (RR₅₀ = 4.8 and 5.9). General esterase, glutathione S‐transferase and monooxygenase levels were also elevated in field‐collected adult and nymph ACP as compared with the LS population. CONCLUSION: The present results suggest that varying levels of insecticide susceptibility exist in ACP populations across the citrus‐growing areas of Florida. Increased levels of detoxifying enzymes in these populations may partially explain these differences. The present results indicate that insecticide resistance may become an emerging problem for ACP control if effective resistance management is not practiced. Copyright © 2011 Society of Chemical Industry     

LncRNA在柑橘木虱与黄龙病病原菌互作中的差异表达分析及验证

为明确柑橘黄龙病的唯一自然传播媒介——柑橘木虱Diaphorina citri的长链非编码RNA(long non-coding RNA,lncRNA)是否参与调控黄龙病病原菌Candidatus Liberibacter asiaticus(CLas)的侵染及复制,采用生物信息学预测及PCR扩增方法进行lncRNA的预测、特征分析、验证及差异表达分析。结果显示,柑橘木虱的13个转录组RNA-Seq数据中共有10 192个lncRNA基因,对应15 747条lncRNA转录本;与蛋白质编码基因相比,柑橘木虱lncRNA具有更少的外显子数量和更短的转录本长度;随机选取的10条lncRNA基因中,有7条lncRNA基因在无菌柑橘木虱广州品系或赣州品系中有表达,其中1条lncRNA基因TCONS_00034665在无菌广州品系和无菌赣州品系中存在差异表达;带菌和无菌柑橘木虱成虫中预测获得2个差异表达的lncRNA基因TCONS_00096118和TCONS_00234564,实时荧光定量PCR验证发现TCONS_00234564与预测结果一致,在带菌柑橘木虱成虫中高表达。表明lncRNA参与了黄龙病病原菌与寄主柑橘木虱的互作。     

Pre-emergence herbicides for the control of broomrape (Orobanche cernua Loefl.) in sunflower (Helianthus annus L.)

Field experiments were conducted from 1989 to 1992 to determine the effects of pre-emergence herbicides in sunflower (Helianthus armuus L.) on: (1) the control of Orobanche cemua Loefl. (broomrape) and (2) crop damage and crop yield. Herbicides tested belong to the imidazolinone, sulfonylurea and substituted amide families. Imazethapy r (20–40 g ha^?1), imazapy r (12.5–25 gha^?1) and chlorsulfuron (4–6 gha^?1) controlled broomrape efficiently without crop injury. With good O. cernua control and good crop tolerance, sunflower seed yield from these treatments were generally similar to the non-infested checks and higher than the infestedchecks. Imazaquin (20–40 gha^?1), triasulfuron(4gha^?1), pdmisulfuron(3g ha^?1), acetochlor (4–4 kg ha^?1) and metazachlor (2 kg ha^?1) were less effective. Imazamethabenz (200–600 g ha^?1) and metolachlor (3–3 kg ha^?1) were ineffective. Wetconditions aftercropsowing considerably decreased O. cemua control with pre-emergence herbicides probably caused by enhanced degrädation. Des herbicides de prelevee pour la lutte contre Vorobanche (Orobanche cemua Loefl.) dans le toumesol (Helianthus annuus L.) Des expérimentations au champ ont été conduites de 1989 á 1992 pour determiner les effets d'herbicides de pré1evée du toumesol sun (a) la destruction de l'orobanche (Orobanche cernua Loefl.) et (b) la phytotoxcité sur la culture. Les herbicides testés appartenaient aux imidazolinones, aux sulfonylurdes et aux amides substituées. L'imazethapyr (20 á 40 gha^?1), l'imazapyr (12,5 á25gha^?1)et le chlorsulfuron (4 á 6 gha^?1) détruisaient efficacement l'orobanche sans occasionner de phytotoxidt6 sur la culture. Dans ces conditions, les rendements étaient généralement semblables à ceux des témoins non infestés et supérieurs à ceux des témoins infestés. L'imazaquin (20 à 40 g ha^?1), le triasulfuron (4 gha^?1), le primisulfuron (3 g ha^?1), lacétolachlor (4,4 kg ha^?1) et le métazachlor (2 kg ha^?1) étaient moins efficaces. L'imazaméthabenz (200 á 600 g ha^?1) et le métolachlor (3,3 kg ha^?1) etaient inefficaces. Des conditions humides aprfes le semis diminuaient considérablement la destruction de O. cemua par les herbicides de prélevée, probablement à cause d'une dégradation plus élevée. Vorauflaufherbigide zur Bekdmpfung der Sommerwurz Orobanche cemua Loefl. in Sonnenblume (Helianthus annuus L.) Zwischen 1989 und 1992 wurden in Sonnenblume Feldversuche zur Wirkung von Vorauflaufherbiziden aus den Gruppen der Imidazolinone, Sulfonylharnstoffe und substitutierten Amide auf die Sommerwurz Orobanche cernua und auf Kulturpflanzenschaden sowie den Ertrag durchgefuhrt. Mit Imazethapyr (20 bis 40 g ha^?1), Imazapyr (12,5 bis 25 g ha^?1) und Chlorsulfuron (4 bis 6 g ha^?1) lieβ sich die Sommerwurz wirksam bekampfen, ohne daβ Schaden an der Sonnenblume auftraten, und die Ertrage waren allgemein ahnlich oder hoher als bei der nichtparasitierten Kontrolle. Imazaquin (20 bis 40 ha^?1), Triasulfuron (4 g ha^?1).Primisulfuron (3 g ha ^?1). Acetochlor (4,4 kg ha ^?1 und Metazachlor (2 kg ha ^?1) waren weniger wirksam. Imazamethabenz (200 bis 600 g ha^?1) und Metolachlor (3,3 kg ha^?1) hatten keine Wirkung. Bei Niederschlagen nach der Saat der Sonnenblume war die Bekampfung der Sommerwurz mit Vorauflaufherbiziden vermutlich wegen verstärkten Abbaus erheblich schwächer.     

Overwintering of the Japanese bayberry whitefly,parabemisia myricae,in Israel

The Japanese bayberry whitefly,Parabemisia myricae (Kuwana), was discovered in Israel on citrus and avocado trees in 1978. In order to clarify its mode of overwintering, observations and counts were carried out during the winters of 1979/80, 1982/83 and 1983/84, with the following findings. Larvae and adults were detected on citrus and avocado trees throughout the winter; only females were found. The oviposition rate ofP. myricae in winter was low, and rose steeply in spring. The density of larvae on the lower side of leaves was higher than on the upper side. Substantial numbers of larvae survived the winter on avocado trees. Emergence of adults became intensified at the end of February, reached a peak in early March, and ceased at the end of March or beginning of April.     

Phosphorous acid treatments control Phytophthora diseases in Australia1

Treatments with a partially neutralized formulation of phosphorous acid containing potassium phosphite were assessed for control of Phytophthora diseases in subtropical and temperate crops in Australia. In Queensland, trunk injections of phosphite (10% solution) controlled severe root rot (Phytophthora cinnamomi) of avocado trees and resulted in the recovery of trees. Single pre-harvest sprays (2.5 kg ha^-1) of phosphite controlled root and heart rot (P. cinnamomi) of pineapples. Foliar sprays of phosphite (64 g per tree) controlled root rot (P. nicotianae var. parasitica) and trunk canker (P. citrophthora) of mandarin trees. In Victoria, a foliar spray of phosphite (300 g ha^-1) reduced root rot (P. clandestina) of subterranean clover and increased dry matter by 1.96 to 5.11 t ha^-1. Trunk injections of phosphite (10% solution) controlled trunk rot (P. cactorum) of peach trees and foliar sprays (10 kg ha^-1) reduced severity of root rot (P. nicotianae var. nicotianae) of tomatoes.