Study on Efficacy of Baihu Dingchuan Oral Liquid

In order to verify the effect of Baihu Dingchuan oral liquid of new veterinary drugs,the effects of infectious bronchitis virus (IBV) on the proliferation inhibition test, tracheal cilia movement test and SPF chickens infected with IBV were tested.The test solution was mixed with an equal amount of IBV solution,inoculation of 11-day-old SPF chicken embryo,incubation at 37℃ for 168 h.RT-PCR was performed to detect IBV in kidney of live chick embryo.The results showed that the survival rate of 168 h chicken embryo was 80% in the drug group,but the positive control group was 20%, and there was significant difference between two groups (P<0.05). In the test drug group, no band was detected by RT-PCR, but the target band appeared in the positive control group.After 40 days old SPF chickens were administered continuously for 3 days, 0.02 mL of ink was injected into the trachea. The distance of ink movement in 1 min was measured. The results showed that the migration distance of ink was extremely significantly higher than that of saline control group (P<0.01).In the 17-day-old SPF chickens, IBV-M41 was infused into the nasal mucosa with 0.3 mL. After 24 h, 1 mL/kg was administered for 5 days. The results showed that there were significant differences in clinical symptom score and cure rate between the test drug group and the control group (P<0.05). There was no significant pathological changes in the tracheal mucosa of the experimental group, but the pathological changes in the positive control group were significant. The above results showed that Baihu Dingchuan oral liquid had good inhibitory effect on IBV in vitro, which could promote the movement of tracheal cilia, and had good clinical control effect on infectious bronchitis.     

白虎定喘口服液的质量检测

为了建立白虎定喘口服液的质量检测标准,采用薄层色谱法(TLC)对处方中金银花、黄芩、知母、板蓝根进行鉴别;采用高效液相色谱法(HPLC)对黄芩中有效成分黄芩苷进行含量测定。结果表明,薄层色谱斑点清晰,分离度好,特异性强;在0.103mg/mL~0.824mg/mL范围内黄芩苷浓度与峰面积呈良好的线性关系,阴性对照无干扰,加样回收率平均为97.98%,精密度试验RSD为0.61%。说明建立的检测方法简便、准确、专属性强、重复性好,可对白虎定喘口服液的质量进行有效检测。     

甘胆口服液

<正>甘胆口服液是一种专用于防制鸡传染性支气管炎、鸡毒支原体感染的纯中药制剂,主要成分为板蓝根、甘草、人工牛黄、猪胆粉、冰片等,本     

传染性支气管炎病毒的鉴定

试验采用鸡胚接种和气管环培养相结合的方法,对IBV毒株进行培养鉴定,IBV经鸡胚传一代后再上鸡胚气管环培养可引起气管环纤毛运动停止,应用此法可以快速地进行IBV检测。     

“强力咳喘灵”对人工感染鸡传染性支气管炎的疗效试验

根据中医辩证施治原则，我们组成了治疗鸡呼吸道疾病的中药方剂“强力咳喘灵”口服液，对其进行了人工感染鸡传染性支气管炎的临床疗效试验，结果表明该药具有良好的预防治疗效果，明显优于西药病毒灵（P＜0．01）。     

柴黄口服液对鸡传染性支气管炎的临床效果

为了观察柴黄口服液对鸡传染性支气管炎(IB)的临床效果，本试验将经病原检测确诊为IB的患病鸡，随机分为2个组，分别为试验组(按2.5 mL/L饮水量给予柴黄口服液+1 g/L饮水量给予20%氟苯尼考粉)和对照组(按1 g/L饮水量给予20%氟苯尼考粉),连续给药5 d,观察用药前后各组鸡只的临床症状、病理剖检变化、脏器指数和组织病理学变化等指标，评价柴黄口服液在常规养殖环境中对鸡IB的治疗效果。结果显示，用药5 d后，与对照组相比，试验组鸡只呼吸道疾病所引起的临床症状减轻，死亡率降低1.7%,平均末重提高18.47%(P<0.01),平均增重提高37.36%(P<0.01);气管内和气囊壁黏性渗出物减少；肺脏、肝脏和脾脏指数稍有升高，但差异不显著(P>0.05)。组织病理学观察结果显示，对照组鸡只的气管、肺脏和肠道等器官组织学结构均发生不同程度的血管扩张充血、黏膜上皮脱落等病变，试验组鸡只的气管、肺脏和肠道器官组织学结构均未见明显异常；2个组鸡只的肾脏均出现肾小球萎缩和肾小管上皮细胞变性坏死等病变。结果表明，柴黄口服液对鸡IB具有较好的治疗作用，其可以保护鸡只气管、肺...     

甘胆口服液

甘胆口服液是一种专用于防制鸡传染性支气管炎、鸡毒支原体感染的纯中药制剂，由北京生泰尔生物科技有限公司研制开发，经大量的临床验证，疗效显著。被农业部批准为国家三类新兽药，证书编号为（2006）新兽药证字74号。     

参灵清瘟败毒口服液对鸡传染性支气管炎的疗效分析

目的：探讨鸡传染性支气管炎采用参灵清瘟败毒口服液防治效果。方法：选取人工感染传染性支气管炎病毒（IBV）标准株M41的海兰褐雏鸡作研究对象,于攻毒前后取3种剂量不同的参灵清瘟败毒口服液在饮水中加入,评价对鸡传染性支气管炎的防治效果。结果：低剂量组死亡率为17.50%、中剂量死亡率为12.50%、高剂量死亡率为12.82%,同抗病毒1号治疗效果相当,明显低于攻毒对照组（P<0.05）,提示针对雏鸡感染IBVM41,采用参灵清瘟败毒口服液治疗,作用显著。结论：针对鸡传染性支气管炎,采用参灵清瘟败毒口服液防治,作用显著,推荐剂量为药液0.75 ml,兑水1 L,在饮水中加入自由饮服,共用5 d,效果理想。     

参灵清瘟败毒口服液防治鸡传染性支气管炎试验

以传染性支气管炎病毒(IBV)标准株M41人工感染3周龄海兰褐雏鸡致其发病,于攻毒前后在饮水中加入3种剂量的参灵清瘟败毒口服液,观察试验鸡的临床症状、病理变化、发病率和死亡率,评价参灵清瘟败毒口服液对鸡传染性支气管炎的预防和治疗效果。结果表明,提前给予参灵清瘟败毒口服液对IBVM41攻击具有保护作用,保护率达86.9%;在攻毒后给予3种剂量的参灵清瘟败毒口服液,可有效治疗人工感染性鸡传染性支气管炎,治愈率达82.3%~87.0%;推荐剂量为0.75 mL药液兑水1 L,自由饮服,连用5 d。     

仔泻康口服液的急性毒性和亚慢性毒性试验研究

试验旨在通过小鼠急性毒性试验和大鼠亚慢性毒性试验对仔泻康口服液进行安全性评价,为临床安全用药提供理论依据。在急性毒性试验中,采用最大给药剂量对36只昆明小鼠进行灌胃给药。在亚慢性毒性试验中,将80只大鼠,随机均分成高、中、低剂量组和对照组,高、中、低剂量组分别按24、12和6 g/kg体重灌胃给药,对照组给予等体积生理盐水,连续给药30 d,停药后称量大鼠体重、检测血常规指标、血液生化指标、计算脏器指数并观察组织病理变化等。结果显示,在急性毒性试验中,各剂量组均无小鼠死亡,无法计算LD₅₀,最大耐受量试验也无死亡情况;在亚慢性毒性试验中,该口服液对大鼠生长发育没有影响;经剖检,仅高剂量组可见中央静脉远端的肝细胞有不同程度的肿大,但未见坏死和炎性反应,其他各剂量组的实质器官均未发现异常变化;各剂量组血液学指标、血液生化指标和脏器指数均在正常范围内,与对照组相比均无显著差异（P>0.05）。结果表明,根据外源化学物急性毒性分级（WHO）标准,该制剂属于无毒物质,安全性较高,在合理剂量下,临床使用仔泻康口服液是安全的。     

鸡支康口服液的亚慢性毒性试验研究

试验根据《兽药试验技术规范汇编》进行了鸡支康口服液对小白鼠的亚慢性毒性研究,试验设高、中、低3个剂量组,另设空白对照组,连续给药28 d后剖杀各组小鼠,观察其临床表现,并进行血常规和血液生化指标检测及脏器系数和组织病毒学检查。统计学分析结果表明,各组间的体重变化和脏器系数均无显著差异(P>0.05);血常规检查结果表明,试验组的红细胞数、血小板数、红细胞压积及淋巴细胞数与空白对照组相比呈下降趋势,但差异不显著(P>0.05),其余各检测指标各试验组与空白对照组相比差异均不显著(P>0.05);血液生化指标结果表明,试验组的谷丙转氨酶、谷草转氨酶及血糖含量与空白对照组相比呈下降趋势,但差异不显著(P>0.05),其余各检测指标各试验组与空白对照组相比差异均不显著(P>0.05);组织病理学检查结果显示,小鼠的主要内脏器官未见明显异常。试验结果表明,鸡支康口服液对小鼠血常规和血液生化指标影响甚微。提示,其按临床剂量使用无毒性反应,安全可靠。     

黄白口服液的质量控制研究

为了制定黄白口服液质量标准,采用薄层色谱(TLC)法对组方中的黄连、金银花、黄芩、地榆炭进行定性鉴别;用高效液相色谱(HPLC)法对制剂中盐酸小檗碱和黄芩苷进行含量测定。结果TLC鉴别专属性强,重现性好;盐酸小檗碱线性范围在0.139~0.834 μg(R²＝0.9999),平均回收率为97.04%,RSD为0.97%(n＝6);黄芩苷线性范围0.235~1.41 μg(R²＝0.9999),平均回收率为97.64%,RSD为0.71%(n＝6)。对黄连、金银花、黄芩、地榆炭的定性鉴别及盐酸小檗碱和黄芩苷的含量测定可用来进行制剂的质量控制。     

清营口服液质量检测方法的研究

为了建立清营口服液的质量检测方法,对制剂处方药味金银花、连翘、黄连、丹参、玄参、麦冬进行了薄层色谱(TLC)鉴别研究,并利用高效液相色谱(HPLC)法对制剂中绿原酸的含量进行了测定确定了HPLC的测定条件。结果表明,TLC鉴别方法专属性强、斑点清晰、阴性对照无干扰;在8~160 μg/mL范围内绿原酸浓度与峰面积呈良好的线性关系,平均回收率为97.86%,RSD为1.2%。以上结果表明,本研究所建立的检测方法简便、准确、专属性强、重复性好,可对清营口服液的质量进行有效检测。     

五皮口服液对小鼠的利尿作用

研究五皮口服液的利尿作用,通过给水负荷模型小鼠灌服不同剂量的五皮口服液,观察给药后的排尿量,以评价五皮口服液的利尿效果。给药后4 h内,与阴性对照组排尿量1.18 g±0.42 g比较,五皮口服液高、中剂量组(13.5 g生药/kg、6.75 g生药/kg体重)排尿量分别为1.78 g±0.42 g、1.73 g±0.60 g,均极显著增加(P<0.01),低剂量组(3.375 g生药/kg体重)的排尿量为1.34 g±0.91 g,显著增加(P<0.05)。结果表明,五皮口服液具有显著利尿作用。     

鱼腥草芩蓝口服液质量控制方法研究

为建立鱼腥草芩蓝口服液的质量控制方法,采用TLC法对鱼腥草芩蓝口服液中的鱼腥草、黄芩、连翘和金银花进行鉴别;采用HPLC法测定黄芩苷和绿原酸的含量。在薄层色谱鉴别中均能检出鱼腥草、黄芩、连翘和金银花的特征性斑点;黄芩苷进样量在0.1057～2.6413μg范围内与峰面积有良好的线性关系,回归方程为:Y=33.42325X-214.6128,r=0.9997,平均回收率为98.74%;绿原酸进样量在0.041～2.051μg/m L范围内与峰面积有良好的线性关系,其回归方程为:Y=-86.78088+27.5937X,相关系数r=0.9995,回归显著,平均回收率为99.1%,建立的黄芩苷和绿原酸含量测定方法,耐受性良好、灵敏度高、重现性好、专属性强,可以有效控制产品的质量。建立的方法能准确、可靠地对鱼腥草芩蓝口服液中黄芩苷和绿原酸含量进行测定,可作为该制剂的质量控制方法。     

速康解毒口服液在试验大鼠体内药代动力学研究

In order to compare the concentration of each component of Sukang detoxification oral liquid in rat plasma after administrated by oral and intramuscular, and ascertain pharmacokinetic characteristics of Sukang detoxification oral liquid in trials rats, 28 rats were randomly divided into seven groups, using UV spectrophotometry to measure plasma concentration of each component at different times after administrated by oral and intramuscular, calculated pharmacokinetics parameters of various components of detoxification agent. The results showed that plasma concentration-time process of each component of detoxification agent in rats could be described as two-compartment model tools with absorption. After oral administration the maximum plasma concentrations of each component were sodium 8039.455 μg/mL, yeast mannan 129.932 μg/mL, magnesium 3358.328 μg/mL, rhamnose 2296.330 μg/mL.Tp(h) by oral administration of sodium,sulfate,yeast mannan,L-rhamnose were 1.2816,1.4201,1.1337,0.9469 h;by intramuscular administration they were 1.2856,1.4684,1.1079,1.1671 h. Experimental studies had shown that each component of Sukang detoxification oral liquid was quickly absorbed into the blood after administration, there was little difference between oral and intramuscular administrations,but oral administration had long function and more convenient on administration than intramuscular.