青海省部分地区PEDV、TGEV和RV感染情况调查与分析

为了解青海省部分地区猪群中猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)和猪轮状病毒(RV)导致猪病毒性腹泻疫病的感染情况与发生现状,本研究首次应用RT-PCR方法对2012年采集的青海省部分地区60份伴有腹泻症状的疑似猪病毒性腹泻病毒感染的临床病料进行猪流行性腹泻病毒、猪轮状病毒和猪传染性胃肠炎病毒的检测与分析.结果显示PEDV、RV和TGEV的阳性率分别为76.7％、51.7％和30.0％;总单独感染率为35.0％,各自单独感染率分别为25.0％、8.33％和1.67％;总混合感染率为51.67％,PEDV/RV、PEDV/ TGEV、PEDV/RV/TGEV的混合感染率分别为23.33％、8.33％和51.67％,不存在RV/TGEV混合感染型.结果表明,青海省部分地区存在这3种导致猪病毒性腹泻疫病病毒的流行,包括单独感染流行和混合感染流行;单独感染中以PEDV流行为主,混合感染中以PEDV/RV和PEDV/RV/TGEV混合感染流行为主;总混合感染率比总单独感染率高.目前青海省部分地区发生的猪病毒性腹泻以PEDV为主要病因,且PEDV/RV、PEDV/RV/TGEV混合感染现象严重,为该地区猪病毒性腹泻的诊断和防控积累了资料.     

贵州省仔猪病毒性腹泻病原学调查

为了解贵州省仔猪病毒性腹泻病原流行情况,对2013—2015年贵州省7个市(州)采集的35个规模养猪场316份仔猪腹泻粪便样品进行病原核酸检测。结果显示,各地均存在猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)和猪轮状病毒(PoRV)感染,PEDV阳性率为62.43%,TGEV阳性率为7.91%,PoRV阳性率为11.76%。不同病原在不同地区呈显著流行趋势,且存在不同程度的混合感染,PEDV与PoRV混合感染率为18.04%,PEDV与TGEV混合感染率为5.06%,TGEV与PoRV混合感染率为3.80%,PEDV、TGEV与PoRV混合感染率为1.90%,近3年主要流行病原为PEDV。为临床防控仔猪病毒性腹泻发生提供了理论依据。     

贵州省部分地区规模化猪场仔猪4种致猪腹泻病病毒感染调查

为了解贵州省规模化猪场猪病毒性腹泻的流行情况,从5个地区9个规模化猪场采集的腹泻仔猪的粪便和病死猪的肠内容物及肠系膜淋巴结共66份,采用PCR或RT-PCR方法,分别进行猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)、猪轮状病毒(RV)和猪圆环病毒2型(PCV-2)的核酸检测。结果显示,PEDV、TGEV、RV和PCV-2检出率分别为72.72%、1.52%、12.12%和3.03%;PEDV/PCV-2、PEDV/RV、PEDV/TGEV混合感染率分别为3.03%、15.2%和1.52%,总混合感染率为19.70%,其中5个猪场为PEDV单独感染。结果表明,贵州省部分地区规模化猪场发生腹泻病的主要病原为PEDV,其次是TGEV、RV和PCR-2,且存在混合感染,为贵州省猪病毒性腹泻的防控提供一定的参考资料。     

我国部分地区原种猪场3种猪腹泻病毒的血清学调查

为了解猪流行性腹泻病毒(PEDV)、猪轮状病毒(PoRV)、猪传染性胃肠炎病毒(TGEV)在我国原种猪场的流行情况,采用ELISA检测方法对2016年1-6月采集的896份发病种猪血清样品进行了血清学检测。结果显示,PEDV、PoRV、TGEV抗体阳性率分别为62.3%,89.0%,23.0%,且存在混合感染情况,其中PEDV+PoRV、PEDV+TGEV、PoRV+TGEV混合感染率分别为42.3%,8.5%,5.6%,PEDV+PoRV+TGEV混合感染率为8.9%;同时对检测样品进行了不同种猪类别的比较和不同日龄段的比较,发现不同类别种猪抗体阳性率和不同日龄种猪抗体阳性率的分布特征显著。结果说明我国原种猪场3种腹泻病毒中PoRV感染率最高,其次是PEDV和TGEV,且在不同种猪类别和不同日龄段的种猪中呈现规律性分布。本试验对国内原种猪场猪血清进行PEDV、PoRV和TGEV抗体阳性率检测,为我国种猪病毒性腹泻的防控提供了参考。     

3种致猪腹泻病毒的多重RT-PCR检测

试验建立了用于检测猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)与猪轮状病毒(PRV)的三重RT-PCR方法,以调查猪群中腹泻病毒感染情况.通过该方法对采自广东省规模化猪场的95份临床疑似病毒性腹泻病料进行了检测,结果表明,猪流行性性腹泻病毒阳性率为33.7%,猪传染性胃肠炎病毒阳性率为4.2%,猪轮状病毒阳性率为20%;猪流行性性腹泻病毒和猪轮状病毒混合感染率为8.4%,猪流行性腹泻病毒和猪传染性胃肠炎病毒混合感染率为2.1%.     

新疆伊犁河谷地区规模化猪场病毒性腹泻疾病检测与分析

为了解2021年伊犁河谷地区规模化猪场猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)、猪轮状病毒(PoRV)、猪德尔塔冠状病毒(PDCoV)及牛病毒性腹泻病毒(BVDV)等5种病毒性腹泻的感染情况,试验采用RT-PCR对2021年从伊犁河谷地区规模化猪场采集患腹泻病猪的血液、粪便及肠内容物等1 200份病料组织检测5种病毒性疾病的感染情况。结果表明,PEDV、TGEV、PoRV、PDCoV、BVDV总阳性率分别为34.5%、7.4%、22.7%、2.9%、8.2%,以PEDV和PoRV阳性率较高,且以二重感染为主,其中PEDV/TGEV、PEDV/PoRV二重感染率分别为7.2%、9.4%,试验为该地区猪场中5种病毒性腹泻疾病的防控提供参考依据。     

河北抚宁地区猪场病毒性腹泻病原的检测与分析

为了解2020—2022年抚宁地区猪场中猪流行性腹泻病毒(PEDV)、猪轮状病毒(PoRV)、猪传染性胃肠炎病毒(TGEV)、猪Delta冠状病毒(PDCov)、猪伪狂犬病病毒(PRV)等5种病毒性疾病引起腹泻的感染情况,本试验采用RT-PCR和PCR方法对采集的患腹泻病猪的血液、粪便、淋巴结、小肠等病料组织1 275份进行5种病毒性疾病的检测。结果表明,种猪以TGEV、PEDV、PRV感染为主、仔猪以TGEV、PoRV、PDCov、PRV感染为主,育肥猪以TGEV、PDCov、PRV感染为主;TGEV、PEDV、PEDV感染以春季和冬季流行,PRV、TGEV感染四季均有感染。,本试验为该地区猪场中5种病毒性腹泻的防控提供参考依据。     

2013-2014年广西省主要猪病毒性传染病流行病学调查

为掌握广西猪主要病毒性传染病流行情况,为猪传染病预防方案提供依据,本研究于2013年1月1日至2014年12月31日从广西省共收集410份样品,运用PCR及RT-PCR方法检测猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型(PCV-2)、猪瘟病毒(CSFV)、猪伪狂犬病毒(PRV)、猪流行性腹泻病毒(PEDV)、传染性胃肠炎病毒(TGEV)、轮状病毒(PORV)、猪流感病毒(SIV)的感染情况。检测结果表明,PRRSV、PCV-2、CSFV、PRV、PEDV、TGEV、 PORV和SIV的平均感染率分别为35.12%、18.54%、1.17%、0.98%、10.00%、2.44%、0和1.22%;PRRSV和PCV-2混合感染率为6.83%;PRRSV在秋、冬季节呈现高感染率为36.67%、45.31%和63.64%、48.78%,而PCV-2在春、夏、冬季节呈现高感染率为44.44%、25.00%,11.29%、19.35%和39.39%、14.63%。PRRSV和PCV-2是混合感染的主要病原,它们互相之间或是与CSFV、PEDV、PRV、SIV及副猪嗜血杆菌、链球菌等混合感染,PRRSV和PCV-2将是今后广西地区猪病防控的重点。     

安徽省仔猪腹泻5种病毒感染情况的调查研究

为了解安徽地区仔猪病毒性腹泻流行病学特点,对从安徽省37个规模化猪场采集腹泻仔猪的粪便或病死猪肠段共计186份,用RT-PCR和PCR技术进行了猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)、轮状病毒(RV)、猪伪狂犬病病毒(PRV)、猪圆环病毒2型(PCV-2)等5种病毒的检测.结果表明,PEDV阳性病料数占59.1％,阳性猪场占75.7％;TGEV阳性病料数占5.4％,阳性猪场占8.1％;RV阳性病料数占2.7％,阳性猪场占2.7％;PRV阳性病料数占8.1％,阳性猪场占21.6％;PCV-2阳性病料数占25.8％,阳性猪场占54.1％.PEDV与TGEV混合感染占4.3％,PEDV与PCV-2混合感染占18.8％,PEDV与PRV混合感染占10.8％,PRV和PCV-2混合感染占4.8％;其他病因50例,占26.9％.结果表明,PEDV、PCV-2 、PRV的单纯或混合性感染是近年安徽省仔猪腹泻的最为重要的3种病毒性病因,说明在今后的仔猪腹泻病防控过程中,应该着重加强对该3种疾病的综合防控.     

2018年～2019年新疆部分地区猪病毒性腹泻病原检测与分析

为了解新疆部分地区的猪病毒性腹泻发病现状和流行情况,本研究于2018年11月～2019年12月对新疆6个不同地区25个疑似病毒性腹泻猪场进行发病时间、发病日龄、发病率与病死率等基本状况调查,并利用RT-PCR方法对采集的疑似病毒性腹泻仔猪的粪便及肠道内容物共1 388份病料样品进行了病毒性腹泻的病原检测。调查结果显示,本研究中的25个猪场的仔猪腹泻主要在冬春季节发生,发病日龄主要为1日龄～6日龄,发病率为80.00%,病死率为87.46%。病原检测结果显示:猪流行性腹泻病毒(PEDV)的阳性率为46.69%,猪传染性胃肠炎病毒(TGEV)的阳性率为6.77%,猪轮状病毒(PoRV)的阳性率为14.55%,猪德尔塔冠状病毒(PDCoV)的阳性率为0.79%。PEDV/TGEV混合感染率为0.43%,PEDV/PoRV混合感染率为2.95%,PEDV/PDCoV混合感染率为0.29%,PEDV/TGEV/PoRV 3种混合感染率为0.43%。结果表明,引起新疆部分地区仔猪病毒性腹泻的主要病原为PEDV,并出现以PEDV/PoRV为主的多种病原混合感染。本研究为新疆地区生猪养殖场科学防控猪流行性腹泻疾病提供了参考。     

Investigation and Analysis of Porcine Epidemic Diarrhea Virus,Transmissible Gastroenteritis Virus and Pseudorabies Virus of Swine Diarrhea Cases in Shandong Province

To understand porcine viral diarrhea prevalence in the large-scale pig farms of Shandong province, a total of 3 035 clinical samples were detected by PCR from January, 2014 to December, 2016.Those samples were detected for porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and pseudorabies virus (PRV). The results showed that the detection rate of PEDV, PRV and TGEV were 67.49%, 9.33% and 3.29%, respectively.During the past three years, the lowest detection rate of PEDV was 48.15% in the fourth quarter of 2014,and the highest was 88.57% in the fourth quarter of 2015.In 2016,the detection rate represented fluctuate declining compared with 2015.The highest positive rate of TGEV was 18.52% in the fourth quarter of 2014,and in the third quarter of 2015 was 15.38%.The lowest positive rate of TGEV was 6.67% in the first quarter of 2016 and TGEV was not detected in the other quarters. The highest detection rate of RPV was 15.68% in the second quarter of 2016,and the lowest was 2.56% in the second quarter of 2014,except the first quarter of 2014 that the PRV was 0. By detecting three kinds of viruses in 69 clinical samples collected passively, the results showed that the detection rate of PEDV,TGEV and PRV were 86.96%,5.80% and 37.68%,respectively. The total single infection rate was 69.57%,the single infection rates of PEDV,TGEV and PRV were 57.97%,1.45% and 10.14%, respectively;The total mixed infection rate was 30.43%,the mixed infection rates of PEDV/PRV,PEDV/TGEV and TGEV/PRV were 26.09%,2.90% and 1.45%, respectively;Obviously, the total single infection rate was higher than the total mixed infection rate. The results showed that the PEDV, PRV and TGEV were prevailing in Shandong province. There were PEDV/TGEV, TGEV/PRV, PEDV/PRV mixed infection, and the number of PEDV/PRV mixed infection was in the majority. However, there was no PEDV/PRV/TGEV mixed type infection. At present, PEDV was the major pathogen of porcine viral diarrhea and the test results could provide the reference to the diagnosis of porcine viral diarrhea.     

Establishment of Double PCR Detection Method of Transmissible Gastroenteritis Virus (TGEV) and Porcine Epidemic Diarrhea Virus (PEDV)

Two pairs of primers used to respectively amplify transmissible gastroenteritis virus (TGEV) S gene and porcine epidemic diarrhea virus (PEDV) M gene were designed to develop a method of differential diagnosis of TGEV and PEDV.The established double PCR could detect S gene of TGEV with the length of 299 bp and M gene of PEDV with the length of 437 bp.Negative results using CSFV,PCV2,PRRSV and PRV as control were obtained.The detection limit of this method was 10⁴ copies/μL.68 clinical samples collected from swine farm were submitted to detect TGEV and PEDV,and the result showed that the established double PCR method with the characteristics of high sensitivity and high specificity could be widely used in clinical diagnosis and epidemiological investigation.     

猪传染性胃肠炎病毒和猪流行性腹泻病毒二重PCR检测方法的建立

通过对猪传染性胃肠炎病毒(TGEV)S基因和猪流行性腹泻病毒(PEDV)M基因进行序列分析,本试验利用DNAStar软件分别设计2对特异性引物,扩增片段长度分别为299和437 bp,建立一种针对TGEV和PEDV感染的二重PCR鉴别诊断方法.该方法能同时检测到TGEV和PEDV,而对猪瘟病毒(CSFV)、猪圆环病毒2型(PCV2)、猪繁殖与呼吸综合征病毒(PRRSV)、猪伪狂犬病病毒(PRV)等均无扩增,其检测TGEV、PEDV的极限为10⁴ 拷贝/μL;用该方法对临床收集的68份疑似病毒性腹泻仔猪粪便和肠道组织样本进行检测,结果表明本试验建立的二重PCR方法具有特异性强、灵敏度高等特点,能用于临床诊断及流行病学调查.     

Investigation on Pathogens of Major Viral Diarrhea in Pig Farms in East China from 2017 to 2019

To investigate the epidemic situation of viral diarrhea in East China in recent years, and provide epidemiological survey data for comprehensive prevention, 549 fecal samples were collected in 2017-2019 from diarrhea pigs in 5 cities of East China and RT-PCR were used to detect porcine epidemic diarrhea virus (PEDV), porcine rotavirus (PoRV), porcine transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV) and porcine Sapelovirus (PSV), and statistical analysis of its positive rate and mixed infection was performed.The results showed that during 2017 and 2019, PEDV,PDCoV,PoRV,TGEV and PSV positive rate were 62.6% (372/594),27.9% (166/594),16.8% (100/594),13.3% (79/594), and 3.87% (23/594), respectively.In the double mixed infection, the average mixed infection rate of PEDV + PDCoV and PEDV + PoRV were the main ones, which were 22.4% (133/594) and 13.3% (79/594), respectively. Among the triple infections, the mixed infection rate of PEDV + PoRV + PDCoV was dominated by 7.58% (45/594), and there were no mixed infections of four and five pathogens. The highest positive detection rate of PEDV was during 2017 and 2019, and mixed infection with PoRV and PDCoV, which was currently the focus of prevention and control of viral diarrhea in East China. TGEV and PDCoV accounted for a large proportion of the detection rate of healthy fattening pigs and sows, indicating that recessive infections had become common and deserve attention from farmers. In addition, the detection rate of PSV was more lower than the other four diarrhea viruses. On the whole, since August 2018 that the farms strengthened biosecurity prevention and control measures, the detection rate of various pathogens and the detection rate of sites have shown a downward trend year by year.     

PEDV、TGEV和PRoV多重RT-PCR检测方法的建立及应用

为建立猪流行性腹泻病毒（PEDV）、猪传染性胃肠炎病毒（TGEV）及猪轮状病毒（PRoV）的快速鉴别检测方法,本试验针对PEDV、TGEV、PRoV的基因组序列设计3对特异性引物PEDV-N、TGEV-M和PRoV-VP6,分别扩增PEDV N基因、TGEV M基因和PRoV VP6基因。经优化反应条件,成功建立了能同时检测并区分PEDV、TGEV、PRoV的多重RT-PCR方法。该方法可特异扩增PEDV、TGEV、PRoV相应的基因片段,而与猪瘟病毒（CSFV）、猪口蹄疫病毒（FMDV）、猪伪狂犬病病毒（PRV）、猪细小病毒（PPV）、猪圆环病毒2型（PCV2）均无交叉反应;对PEDV、TGEV、PRoV基因重组质粒标准品的检出限分别为1.41×10³、1.41×10²和1.41×10³拷贝/μL;在相同条件下重复试验可获得一致的结果。应用该方法对临床采集的190份腹泻病料进行检测,结果PEDV阳性42份,阳性率22.11%;TGEV阳性58份,阳性率30.53%;PRoV阳性34份,阳性率17.89%,且存在不同病毒混合感染的现象。结果表明,所建立的多重RT-PCR方法具有特异性强、敏感性高、重复性好的优点,可用于PEDV、TGEV和PRoV的临床检测和流行病学调查。     

2017—2019年华东地区猪场主要病毒性腹泻病原调查

本研究旨在了解当前我国华东地区引起猪腹泻的主要病毒性腹泻病原的流行情况，为该地区更好地开展猪腹泻病毒的防控工作提供临床数据。分别采用RT-PCR检测方法对2017—2019年华东地区35个场别594份临床样品检测猪流行性腹泻病毒（porcine epidemic diarrhea virus，PEDV）、猪轮状病毒（porcine rotavirus，PoRV）、猪传染性胃肠炎病毒（transmissible gastroenteritis virus of swine，TGEV）、猪丁型冠状病毒（porcine deltacoronavirus，PDCoV）和猪萨佩罗病毒（porcine Sapelovirus，PSV）等5种猪的病毒性腹泻病原，并对其阳性率及混合感染情况进行统计分析。结果显示，2017—2019年的PEDV、PDCoV、PoRV、TGEV及PSV的平均阳性率为分别为62.6%（372/594）、27.9%（166/594）、16.8%（100/594）、13.3%（79/594）及3.87%（23/594），二重混合感染中以PEDV+PDCoV及PEDV+PoRV为主，平均混合感染率分别为22.4%（133/594）和13.3%（79/594）。三重感染中以PEDV+PoRV+PDCoV混合感染为主，感染率为7.58%（45/594），未见四重及五重病原混合感染。2017—2019年PEDV平均阳性检出率最高，且常与PoRV及PDCoV发生混合感染，是当前华东地区猪病毒性腹泻类疫病的防控重点。TGEV和PDCoV在健康育肥猪及母猪的检出率中占比较大，表明隐性感染变得普遍，值得养殖户关注。除此之外，PSV的检出率远低于其他4种腹泻病毒。总之，随着2018年后养殖场加强生物安全防控措施和猪群管理，各病原检出率和阳性场检出率呈现逐年下降趋势。     

Establishment and Application of a Multiplex RT-PCR Assay for Detection of PEDV,TGEV and PRoV

In this study,a multiplex RT-PCR assay was established to differentially detect porcine epidemic diarrhea virus (PEDV),porcine transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PRoV) after optimization of the reaction conditions.Three pairs of primers PEDV-N,TGEV-M and PRoV-VP6 were designed for specifically amplifying PEDV N gene,TGEV M gene and PRoV VP6 gene,respectively.The assay could specifically amplify PEDV,TGEV and PRoV,but not classical swine fever virus (CSFV),porcine foot and mouth disease virus (FMDV),pseudorabies virus (PRV),porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2).The detection limits of PEDV,TGEV and PRoV standard recombinant plasmids were 1.41×10³,1.41×10² and 1.41×10³ copies/μL,respectively.The repeated reaction under the same conditions obtained uniform results.The assay was used to detect a total number of 190 clinical samples,of which 42 (22.11%) samples were positive for PEDV,58 (30.53%) samples for TGEV and 34 (17.89%) samples for PRoV,and there were mixed infection among these viruses.The results indicated that this multiplex RT-PCR assay had the advantages of sensitivity,specificity and repeatability and provided a useful tool for differential detection and epidemiological investigation of PEDV,TGEV and PRoV.     

Establishment and Application of Duplex RT-PCR Assay for Detection of Porcine Epidemic Diarrhea Virus and Pseudorabies Virus

In order to establish an assay for detecting porcine epidemic diarrhea virus (PEDV) and pseudorabies virus (PRV),two pairs of primers were designed basing on the M gene of PEDV and gE gene of PRV,respectively.The total RNA of standard PEDV and PRV strains were used as templates to establish the duplex RT-PCR assay.The specificity,sensitivity,repetition and clinic detection of the established assay were tested.The result revealed that the threshold of duplex RT-PCR was 10 TCID₅₀/mL of PEDV and PRV,and no products were amplified from the cell or the nucleic acid of other 7 kinds of pathogenic viral or bacterial microorganism.The detection results for 26 clinical suspicious PEDV or PRV infected pigs were consistent with the results tested by sequencing.This study suggested that the duplex RT-PCR method was highly specific,repeatable and sensitive,and was suitable for clinic rapid differential diagnosis of PEDV and PRV.     

猪流行性腹泻病毒与猪伪狂犬病病毒二重RT-PCR检测方法的建立及应用

为建立猪流行性腹泻病毒（PEDV）与猪伪狂犬病病毒（PRV）的快速鉴别检测方法,本研究根据GenBank已登录的PEDV膜蛋白M基因和PRV gE基因保守区域序列设计了2对特异性引物,以PRV和PEDV混合总RNA为反转录模板,初步建立了PRV和PEDV的二重RT-PCR检测方法,并进行了特异性、敏感性、重复性验证和临床应用检测。结果显示,该方法对两种病毒的最低检测限均为10 TCID₅₀/mL病毒含量,重复性好,特异性强,可特异性地扩增PEDV和PRV细胞培养物,但对其他7种病原对照扩增不出任何条带,对26份临床疑似PEDV和PRV感染样品检测结果与测序鉴定结果完全一致。本研究成功建立了PEDV和PRV的二重RT-PCR检测方法,为临床上猪流行性腹泻和猪伪狂犬病的快速鉴别诊断提供了方法。     

猪传染性胃肠炎与流行性腹泻病毒二重RT-PCR 检测方法的建立及应用

为建立猪传染性胃肠炎病毒(TGEV)与流行性腹泻病毒(PEDV)的快速鉴别诊断方法,本研究根据GenBank已登录的TGEV核蛋白(N)基因和PEDV膜蛋白(M)基因保守区域序列分别设计了1对特异性引物,以TGEV和PEDV混合总RNA为反转录模板,建立了TGEV和PEDV的二重RT-PCR检测方法,并进行了特异性、敏感性和重复性试验;利用所建立的检测方法对临床疑似样品进行了应用检测,并对检测到的阳性样品进行克隆测序。结果表明,成功建立了TGEV和PEDV二重RT-PCR检测方法,该方法的检测灵敏度最低极限为10 TCID₅₀/mL病毒含量,重复性好,特异性强,可特异性地扩增TGEV和PEDV细胞培养物,但对ST细胞和其他7种病原对照扩增不出任何条带;对22份临床疑似TGEV和PEDV感染样品检测结果与测序结果完全一致。本研究成功建立了TGEV与PEDV二重RT-PCR检测方法,可适用于猪传染性胃肠炎和流行性腹泻病的快速鉴别诊断。