Response of potato cell cultures to aluminium and selection of tolerant cell lines

Summary We developed a method for the selection of aluminium-tolerant cell lines by using a liquid medium which more closely simulates acid soil conditions than media used previously. This medium has a pH of 4.8 and contains Al₂(SO₄)₃ instead of Al-EDTA as the selective agent to avoid the toxic effects of EDTA. It is shown that Al₂(SO₄)₃ exhibits a similar toxic effect on the growth of intact plants and cultured cells. With this medium, potato cell lines tolerating 2 mmol/l aluminium have been selected.     

Influence of regeneration and selection procedures on the production of aluminium tolerant potato regenerants

Summary Tests were made of the influence of selective and nonselective culture media on the rate of production of aluminium tolerant regenerant clones. Regenerants died when selective medium containg Al was continuously applied in tolerance tests. The production of aluminium tolerant clones was dependent on alternating selective and nonselective media. Tests using a medium containing 6 mmol al resulted in 50% growth as compared to control plants. Regenerants showed no growth when a medium with 10 mmol Al was applied. No constant tolerance was obtained when only a nonselective medium was used for regneration.     

Aluminium-tolerant regenrants from potato cell cultures

Summary Cell cultures were developed from dihaploid clones ofSolanum tuberosum L. Selection in cell suspensions as well as plating of cells on selective medium supplemented with 5 mmol Al produced tolerant cell lines. The constancy of Al-tolerance of cell lines was confirmed by culturing the calli for 3 months in Al-free medium and then transferring them back to selective medium. 4 tolerant regenerant clones were obtained which maintained Al-tolerance also after subculture in control medium. Two of the 4 clones that constantly maintained Al-tolerance, originated from cell lines subcultured for 5 months under stress conditions. However, the regeneration rate of these cell lines was low compared with that of lines obtained after a shorter selection period.     

电子束辐射诱变及克隆选择培育好好芭抗寒无性系的研究

利用电子辐射处理好好芭试管苗，从改变基因型入手提高其抗寒力，以获得稳定遗传的好好芭株系。研究表明150GY的辐射量是诱发好好芭发生变异的最佳剂量，结合生化标记，抗寒相关的酶类——淀粉酶和过氧化物酶活性分析，人工模拟低温实验的克隆检测，筛选出了具有较高抗寒能力的好好芭株系，这些株系通过无性系繁殖可用于抗寒育种。     

Response of three potato cultivars to inoculation withAzotobacter

Summary When the potato cultivars Arran Banner, Bintje, and Compagnon were inoculated withAzotobacter chroococcum in Libya, only Arran Banner responded significantly; plant growth was stimulated and the yield of marketable tubers increased by 8.5–42.6% above the untreated control. The very large yield increase in one season was associated with unfavourable growing conditions.

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Zusammenfassung Im Herbst 1975, im Frühjahr und im Herbst 1976 wurden in Tripolis. Libyen, drei Versuche durchgeführt, um den Einfluss der Inokulation von Pflanzkartoffeln mitAzotobacter auf das Wachstum und den Ertrag von drei Kartoffelsorten (Arran Banner, Bintje und Compagnon) zu untersuchen. Nur bei der Sorte Arran Banner ergab sich eine Beeinflussung durch die Inokulation mitAzotobacter (Tabelle 1 und 3). Der Ertrag war in den drei Versuchen um 42.6% 17.4% bzw. 8.5% gesteigert. Die st?rkste Reaktion ergab sich im ersten Versuch. bei dem niedrige Temperaturen und Bew?lkung vorherrschten und die Wachstumsbedingungen für die Kartoffeln im allgemeinen ungünstig waren. Die Sorten Bintje und Compagnon zeigten keine signifikante Steigerung des Ertrages nach der Inokulation. Die Inokulation erh?hte die Stengell?nge, hatte aber keinen signifikanten Einfluss auf die Stengelzahl pro Pflanze (Tabelle 2). Z?hlungen im Boden der Rhizosph?re zeigten. dass nach Inokulation des Pflanzgutes mitAzotobacter die Population in der Wurzelzone anstieg (Tabelle 4). Die Ertragssteigerung nach der Inokulation scheint durch eine Anreicherung vonAzotobacter in der Rhizosph?re intokulierter Pflanzen hervorgerufen zu sein.

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Résumé Trois expérimentations ont été menées à Tripoli en Libie afin d'étudier l'effet de l'inoculation de tubercules de semence avecAzotobacter sp. sur la croissance et le rendement de 3 variétés de pomme de terre (Arran Banner, Bintje et Compagnon). Ces essais se sont déroulés durant l'automne 1975, le printemps et l'automne 1976. Il n'y a eu que la variété Arran Banner qui a répondu de manière conséquente à l'inoculationd'Azotobacter sp. (tableaux 1 et 3). L'augmentation de rendement a été respectivement de 42.6%, 17.4% et 8,5% dans les 3 expériences. La meilleure réponse a été obtenue dans la première expérimentation où des températures basses et des jours couverts ont dominé et où les conditions de végétation ont été généralement défavorables à la pomme de terre. Les variétés Bintje et Compagnon n'ont pas montré d'augmentation significative du rendement avec inoculation. L'inoculation parAzotobacter sp, augmente la longueur des tiges, mais n'a pas d'effet significatif sur le nombre de tiges par plante (tableau 2). Les comptagesd'Azotobacter sp. dans la rhizosphère ont indiqué que l'inoculation de la semence augmentait la populationd'Azotobacter au niveau de la zone racinaire (tableau 4). Cela est probablement d? au fait que l'augmentation du rendement grace à l'inoculation résulte d'une meilleure adaptationd'Azotobacter sp. au niveau de la rhizosphère des plantes inoculées.

     

Response of secondary selection on soybean cyst nematode reproduction on resistant soybean lines

Soybean cyst nematode, Heterodera glycines Ichinohe, (SCN) populations have been developed which are capable of reproducing on soybean lines known to be resistant to one or more SCN races. Three of the populations developed on PI 88788, PI 90763 and Peking were selected for their differential response on the three hosts. The secondary selection was made on the parent which originally had a high level of resistance. With one exception, secondary selection resulted in increased reproductive ability of the populations on the soybean lines on which they were selected. There was also a corresponding decrease in the reproductive ability on the soybean line on which they were originally developed. The genes from PI 88788, PI 90763 and Peking could be used alternately to provide protection against SCN damage.     

Response of potato tubers to hypoxia followed by re-aeration

Summary Potato tubers kept under hypoxia (1%) showed improved viability in comparison to anoxia, which was associated with the maintenance of intermediate adenylate energy charge values (A.E.C.=0.6) and stable adenylate pools at 50% of the initial levels. Re-admission of oxygen to the tuber resulted in an almost full recovery of adenylate energy charge and total adenylates after up to 3 days of hypoxic pretreatment. Tubers exhibited a mixed fermentation. The high lactate, ethanol and acetaldehyde levels proved to be non toxic. Ethanol was degraded to acetaldehyde during re-aeration. Posthypoxic lipid peroxidation was indicated by malondialdehyde and ethane formation. Both products occurred with a temporary delay and in lower amounts compared to post-anoxia. Ethylene release was also considerably smaller. Severe hypoxia and posthypoxia postponed tissue death compared to anoxia. Survival was correlated with an improved energy supply which stabilized membranes.     

Response of two sweet potato cultivars to weed interference

Field experiments were conducted at the U.S. Vegetable Laboratory, Charleston, SC, U.S.A in 2000, 2001, and 2004 to assess the effect of different durations of weed interference on two sweet potato cultivars with different shoot growth habits. The cultivars were Beauregard, which has a spreading growth habit that is typical of U.S. sweet potato cultivars and Carolina Bunch, with an erect growth habit. Weed interference treatments included control plots that were maintained weed free throughout the growing season and plots that were maintained weed free for 0, 10, 20, and 30 days after transplanting. In general, Carolina Bunch was more tolerant of weed interference than was Beauregard. In two of three years Carolina Bunch storage root yields were higher than Beauregard yields in plots that received no weeding; whereas, yields of the two cultivars in weed free plots were not different. Weed interference affected shoot growth to a greater extent than it affected storage root production. At the end of the growing season, Carolina Bunch shoot biomass was greater than Beauregard shoot biomass in plots receiving no weeding and in plots that were maintained weed free for 10 days in all three years. Shoot biomasses of the two cultivars were not different in weed-free plots. Weed shoot biomasses were greater in Beauregard plots than in Carolina Bunch plots in several instances. These results demonstrate that sweet potato cultivars with a vigorous, erect shoot growth habit (with shorter stems, greater branching, and a denser and taller canopy early in the growing season) may be less susceptible to weed interference than cultivars with spreading shoot growth. This study also confirmed previous observations that sweet potato productivity is not greatly affected by moderate weed interference; thus, it may not require intense weed management to produce high yields.     

Response of six wild potato species to chloride and sulfate salinity

Eleven accessions belonging to six species ofSolanum (S. bulbocastanum,S. chacoense, S. gourlayi, S. microdontum, S. papita, and S.sparsipilum) were tested for resistance to salt. Resistance was measured by survival and growth (dry weight of tops) of plants treated with solutions of NaCl and Na₂SO₄. The six species could be grouped into four classes based on good to poor resistance to salt solutions (1.S. chacoense, 2.S. gourlayi, 3.S. microdontum, S. sparsipilum, S. bulbocastanum and 4.S. papita). There was a close relationship (r=0.94**) between the survival of plants and growth of surviving plants treated with salt solutions. There was no relationship between known heat or drought resistance of various accessions and salt resistance. Results indicate there is a large genetic base available to improve the salt resistance of the cultivated potato.     

Response of potato cultivars to North American isolates of PVYNTN

Potato cultivars were assessed for their sensitivity to potato tuber necrotic ringspot disease (PTNRD) induced by two North American isolates (Tu619 and Tu660) of the tobacco veinal necrosis strain of potato virus Y (PVY^N) and belonging to the tuber necrosis (PVY^NTN) sub-group. Several widely grown cultivars (e.g. Norchip and Ranger Russet) were found to be highly sensitive to PTNRD, while others (e.g. Russet Burbank, Russet Norkotah and Shepody) were not. The foliage symptoms induced by these isolates were distinct from those reported to be produced by European isolates of PVY^NTN, in that systemic necrotic rings and chlorophyll figures on the leaves were not produced. Instead, the symptoms were similar to those of normal strains of PVY^N. The apparent correlation between resistance to the PVY^O (common) strain group and cultivar sensitivity to PTNRD is discussed.     

Response of four potato cultivars to rate and timing of nitrogen fertilizer

The release of three new potato (Solarium tuberosum L.) cultivars, Bannock Russet, Gem Russet, and Summit Russet, with unique plant growth characteristics, necessitates the development of appropriate N fertilizer recommendations. These three new cultivars, along with the standard cultivar, Russet Burbank, were treated with four N rates (0, 100, 200, and 300 kg N ha^?1) using two different application timing procedures (“early,” with two-thirds N applied preplant, and “late,” with one-third applied preplant). Measurements included total and U.S. No. 1 yields, petiole NO₃-N concentrations, and net returns derived from economic analysis using a processing-based contract. Each of the four cultivars showed a unique response to N application treatments. Bannock Russet achieved maximum yield and net returns with relatively small amounts of N fertilizer. It also showed no response to N application timing and had moderate NO₃-N sufficiency concentrations early in the season, that decreased markedly late in the season. Gem Russet N requirement for maximum yield was similar to that of Russet Burbank, but required a higher amount of N for maximum net returns. Gem Russet also showed no response to application timing and had NO₃-N sufficiency concentrations similar to or slightly higher than those of Russet Burbank. Summit Russet showed a strong trend for improved N use-efficiency when most of the N was applied early. On the other hand, analysis of net returns revealed a trend for greater profitability for Summit Russet when the majority of N was applied during tuber bulking. Petiole NO₃-N sufficiency concentrations for Summit Russet were generally higher than those for the other three cultivars. In comparison with some earlier studies with Russet Burbank, this research suggested lower optimal N rates and petiole NO₃-N sufficiency concentrations.     

Genetic variation in a population of tetraploid potatoes: Response to the potato leafhopper and the potato flea beetle

Progenies from a group of tetraploid parental clones from the USDA potato breeding program were used to investigate variation in resistance to the potato leafhopper,Empoasca fabae (Harris), and the potato flea beetle,Epitrix cucumeris (Harris). The study utilized two mating designs: i) selfing and testing both the parents and the S₁ progeny; and ii) nine clones, used as males, were each crossed to three different clones, and progenies from the resulting families were tested. Statistically significant differences between families were measured in each test for both insects. Non-additive genetic variance was larger than additive genetic variance in progeny reaction to leafhopper infestation and hopperburin, but was smaller in progeny reaction to flea beetles. Environmental variation contributed heavily to the total variation of plant reaction to both insect species. Selecting individuals was indicated to be slightly more effective than selecting males on half-sib progeny performance but not as effective as selecting clones on S₁ progeny performance. Because of the large environmental variance and small additive variance for both the leafhopper and flea beetle, slow progress in increasing the level of resistance to these two species in this sample population was predicted. Resistance to leafhopper infestation was genetically quite highly correlated (positive) with resistance to hopperburn, but phenotypically the correlation was considerably smaller. Negative genotypic, phenotypic, and environmental correlations between leafhopper infestation and flea beetle infestation suggest that selecting for resistance to one of these species, in the population sampled for these tests, would tend to increase susceptibility to the other.     

Evaluation of dihaploid populations from potato varieties and breeding lines

Summary Dihaploid populations from 20 varieties and 11 breeding lines ofSolanum tuberosum differed greatly in the occurrence and frequency of mutant phenotypes, in tuberization, flowering, pollen stainability, 2n-pollen production and resistance toGlobodera rostochiensis pathotype Ro-1. One population contained up to 61.7% lethal mutants: 39.1% of the total of 5377 dihaploids obtained did not tuberize, and 32.4% of 825 vigorous dihaploids did not flower. Of 825 vigorous dihaploids, 26.9 and 3.9% respectively had a pollen stainability higher than 20 and 60%. Among 222 dihaploids with a pollen stainability higher than 20%. 23.0% produced some 2n-pollen and none of these had a stainability higher than 60%. Among the dihaploid populations investigated the proportion of resistant to susceptible dihaploids ranged from 8:26 to 62:8.     

Early generation selection of insect resistance in potato

Methods used in breeding and selecting potatoes for resistance to Colorado potato beetle, green peach aphid, potato aphid, and potato leafhopper are reviewed. Sources of resistant germplasm, the nature of resistance, breeding behavior of resistance factors, and methods used to measure resistance are discussed. Specific problems encountered in breeding insect-resistant potatoes are discussed in relation to early-generation selection procedures.     

Selection for resistance to potato leafhopper in potatoes I.selection methods

A randomly mated potato (Solarium tuberosum L.) population (3 generations, without selection) derived from clones from several breeding programs, displayed considerable resistance in the field to potato leafhopper,Empoasca fabae (Harris). Leafhopper population levels on 161 clones ranged from 2 to 28 nymphs per 45-sec count (mean = 12), whereas a susceptible check averaged 20 nymphs per count. Clones selected for low leafhopper counts in 1971 were compared with the population in 1972 to evaluate the effectiveness of selecting from small plots (three-hill) with minimum replication. The selected clones averaged 30% fewer leafhoppers than the population average in 1971 and 10% fewer in 1972. The average heritability (broad sense) for one plot per clone per location, two locations, and two 45-sec nymph counts per plot equaled 36%.