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1.
Influence of inorganic fertilizers and organic amendments on soil organic matter and soil microbial properties under tropical conditions 总被引:33,自引:0,他引:33
Soil organic matter level, mineralizable C and N, microbial biomass C and dehydrogenase, urease and alkaline phosphatase
activities were studied in soils from a field experiment under a pearl millet-wheat cropping sequence receiving inorganic
fertilizers and a combination of inorganic fertilizers and organic amendments for the last 11 years. The amounts of soil organic
matter and mineralizable C and N increased with the application of inorganic fertilizers. However, there were greater increases
of these parameters when farmyard manure, wheat straw or Sesbania bispinosa green manure was applied along with inorganic fertilizers. Microbial biomass C increased from 147 mg kg–1 soil in unfertilized soil to 423 mg kg–1 soil in soil amended with wheat straw and inorganic fertilizers. The urease and alkaline phosphatase activities of soils
increased significantly with a combination of inorganic fertilizers and organic amendments. The results indicate that soil
organic matter level and soil microbial activities, vital for the nutrient turnover and long-term productivity of the soil,
are enhanced by use of organic amendments along with inorganic fertilizers.
Received: 6 May 1998 相似文献
2.
Bioavailability and effects of heavy metals on soil microbial biomass survival during laboratory incubation 总被引:13,自引:0,他引:13
L. Leita M. De Nobili G. Muhlbachova C. Mondini L. Marchiol G. Zerbi 《Biology and Fertility of Soils》1995,19(2-3):103-108
In this work we studied the influence of Pb, Zn, and Tl on microbial biomass survival and activity during a laboratory incubation of soil. In comparison to uncontaminated soil, the microbial biomass C decreased sharply in soil contaminated with Zn and Tl, whereas the addition of Pb did not have any significant inhibitory effect on the level of microbial biomass C. Zn displayed the greatest biocidal effect, confirmed by the measurement of the death rate quotient (q D). The microbial activity, measured as CO2 evolution, increased significantly in contaminated soils, emphasizing the need of living organisms to expend more energy to survive. The greater demand for energy by microorganisms in order to cope with the toxicity of pollutants was also confirmed by measurement of the metabolic quotient (q CO2). In order to determine whether soil microorganisms affect the bioavailability of these metals through their mobilization and release, we studied the relationships between available Pb, Zn, and Tl, and microbial biomass C. The water-soluble fraction of Tl, available Tl, and Zn, and microbial biomass C were related significantly, but not Pb. 相似文献
3.
Influence of heavy metals on the functional diversity of soil microbial communities 总被引:40,自引:0,他引:40
Three soil types-Calcaric Phaeozem, Eutric Cambisol and Dystric Lithosol-in large container pots were experimentally contaminated with heavy metals at four different levels (light pollution: 300 ppm Zn, 100 ppm Cu, 50 ppm Ni, 50 ppm V and 3 ppm Cd; medium pollution: twofold concentrations; heavy pollution: threefold concentrations; uncontaminated control). We investigated the prognostic potential of 16 soil microbial properties (microbial biomass, respiration, N-mineralization, 13 soil enzymes involved in cycling of C, N, P and S) with regard to their ability to differentiate the four contamination levels. Microbial biomass and enzyme activities decreased with increasing heavy metal pollution, but the amount of decrease differed among the enzymes. Enzymes involved in the C-cycling were least affected, whereas vartous enzyme activities related to the cycling of N, P and S showed a considerable decrease in activity. In particular, arylsulfatase and phosphatase activities were dramatically affected. Their activity decreased to a level of a few percent of their activities in the corresponding unpolluted controls. The data suggest that aside from the loss of rare biochemical capabilities-such as the growth of organisms at the expense of aromatics (Reber 1992)-heavy metal contaminated soils lose very common biochemical propertities which are necessary for the functioning of the ecosystem. Cluster analysis as well as discriminant analysis underline the similarity of the enzyme activity pattern among the controls and among the polluted soils. The trend toward a significant functional diversity loss becomes obvious already at the lowest pollution level. This implies that concentrations of heavy metals in soils near the current EC limits will most probably lead to a considerable reduction in decomposition and nutrient cycling rates. We conclude that heavy metal pollution severely decreases the functional diversity of the soil microbial community and impairs specific pathways of nutrient cycling.Dedicated to Professor J. C. G. Ottow on the occasion of his 60th birthday 相似文献
4.
Effects of long-term waste water irrigation on soil organic matter, soil microbial biomass and its activities in central Mexico 总被引:4,自引:0,他引:4
The effect of long-term waste water irrigation (up to 80 years) on soil organic matter, soil microbial biomass and its activities
was studied in two agricultural soils (Vertisols and Leptosols) irrigated for 25, 65 and 80 years respectively at Irrigation
District 03 in the Valley of Mezquital near Mexico City. In the Vertisols, where larger amounts of water have been applied
than in the Leptosols, total organic C (TOC) contents increased 2.5-fold after 80 years of irrigation. In the Leptosols, however,
the degradability of the organic matter tended to increase with irrigation time. It appears that soil organic matter accumulation
was not due to pollutants nor did microbial biomass:TOC ratios and qCO2 values indicate a pollutant effect. Increases in soil microbial biomass C and activities were presumably due to the larger
application of organic matter. However, changes in soil microbial communities occurred, as denitrification capacities increased
greatly and adenylate energy charge (AEC) ratios were reduced after long-term irrigation. These changes were supposed to be
due to the addition of surfactants, especially alkylbenzene sulfonates (effect on denitrification capacity) and the addition
of sodium and salts (effect on AEC) through waste water irrigation. Heavy metals contained in the sewage do not appear to
be affecting soil processes yet, due to their low availability. Detrimental effects on soil microbial communities can be expected,
however, from further increases in pollutant concentrations due to prolonged application of untreated waste water or an increase
in mobility due to higher mineralization rates.
Received: 28 April 1999 相似文献
5.
Changes in soil microbial biomass, metabolic quotient, and organic matter turnover under Hieracium (H. pilosella L.) 总被引:9,自引:0,他引:9
In New Zealand Hieracium is an opportunistic plant that invades high country sites more or less depleted of indigenous vegetation. To understand the
invasive nature of this weed we assessed the changes in soil C, N and P, soil microbial biomass C, N and P contents, microbial
C : N and C : P ratios, the metabolic quotient, and turnover of organic matter in soils beneath Hieracium and its adjacent herbfield resulting from the depletion of tussock vegetation. The amounts of soil organic C and total N
were higher under Hieracium by 25 and 11%, respectively, compared to soil under herbfield. This change reflects an improvement in both the quantity and
quality of organic matter input to mineral soil under Hieracium, with higher percentage organic C and a lower C : N ratio. The microbial biomass C, N and P contents were also higher under
Hieracium. The amount of C respired during the 34-week incubation indicated differences in the nature of soil organic matter under
Hieracium, the unvegetated "halo" zone surrounding Hieracium patches, and herbfield (depleted tussock grassland). Decomposition of organic matter in these zones showed that the Hieracium soil had the greatest rate of CO2 respired, and the halo soil had the lowest. We relate the enhanced organic C turnover to the invasive nature of Hieracium. Net N mineralization was significantly lower from the Hieracium soil (57 mg N g–1 soil N) than from herbfield and halo soils (74 and 71 mg N g–1 soil N, respectively), confirming that the nature of organic N in Hieracium soil is different from adjoining halo and herbfield soils. It seems plausible that specific compounds such as polyphenols
and lignins released by Hieracium are not only responsible for increased organic N, but also control the form and amount of N released during organic matter
transformations. We conclude that the key to the success of Hieracium in the N-deficient South Island high country of New Zealand lies in its ability to control and sequester N supply through
modifying the soil organic matter cycle.
Received: 1 December 1998 相似文献
6.
Roberto Alvarez Oscar J. Santanatoglia Roberto Garcîa 《Biology and Fertility of Soils》1995,19(2-3):227-230
Variations in the microbial biomass and the in situ metabolic quotient (qCO2) due to climatic conditions were determined in a typical soil from the Argentine Rolling Pampa. Microbial C was evaluated by fumigation-incubation and qCO2 was calculated using soil respiration in the field. An inverse relationship between microbial C and soil temperature was fitted to a model (r
2=0.90, P=0.01). No significant association with the soil water content was detected because the soil was generally near field capacity and thus water availability did not limited microbial growth and activity. Values of qCO2 increased (r
2=0.89, P=0.01) as the result of metabolic activatìon, likely induced by a higher maintenance energy requirement at high temperatures. The highest values of qCO2 were obtained when microbial C was the lowest, which was attributed to self consumption of microbial C in the presence of high temperatures. Consequently, microbial C was generally higher (P=0.05) in winter than in summer. Therefore, when microbial C is used as an index of soil biological activity, the influence of temperature should be taken into account. 相似文献
7.
R. J. Haynes 《Biology and Fertility of Soils》1999,30(3):210-216
The effects of 5 years of continuous grass/clover (Cont grass/clover) or grass (Cont grass) pasture or 5 years of annual
grass under conventional (Ann grass CT) or zero tillage (Ann grass ZT) were compared with that of 5 years of continuous barley
(LT arable) on a site which had previously been under arable crops for 11 years. For added comparison, a long-term grass/clover
pasture site (LT past) nearby was also sampled. Soil organic C (Corg) content followed the order LT arable=Ann grass CT<Ann grass ZT<Cont grass=Cont grass/clover<LTpast. Trends with treatment
for microbial biomass C (Cmic), basal respiration, flourescein diacetate (FDA) hydrolytic activity, arginine ammonification rate and the activities of
dehydrogenase, protease, histidase, acid phosphatase and arylsulphatase enzymes were broadly similar to those for Corg. For Cmic, FDA hydrolysis, arginine ammonification and the activities of histidase, acid phosphatase and arylsulphatase, the percentage
increase caused by 5 years of continuous pasture (in comparison with LT arable) was 100–180%, which was considerably greater
than that for organic C (i.e. 60%). The microbial metabolic quotient (qCO2) was higher for the two treatments which were mouldboard ploughed annually (LT arable and Ann grass CT) than for the undisturbed
sites. At the undisturbed sites, Corg declined markedly with depth (0–15 cm) and there was a similar stratification in the size and activity of Cmic and enzyme activity. The microbial quotient (Cmic/Corg) declined with depth whilst qCO2 tended to increase, reflecting a decrease in the proportion of readily available substrate with depth.
Received: 7 July 1998 相似文献
8.
Structure and function of the soil microbial community in microhabitats of a heavy metal polluted soil 总被引:26,自引:0,他引:26
E. Kandeler D. Tscherko K. D. Bruce M. Stemmer P. J. Hobbs R. D. Bardgett W. Amelung 《Biology and Fertility of Soils》2000,32(5):390-400
Particle-size fractionation of a heavy metal polluted soil was performed to study the influence of environmental pollution
on microbial community structure, microbial biomass, microbial residues and enzyme activities in microhabitats of a Calcaric
Phaeocem. In 1987, the soil was experimentally contaminated with four heavy metal loads: (1) uncontaminated controls; (2)
light (300 ppm Zn, 100 ppm Cu, 50 ppm Ni, 50 ppm V and 3 ppm Cd); (3) medium; and (4) heavy pollution (two- and threefold
the light load, respectively). After 10 years of exposure, the highest concentrations of microbial ninhydrin-reactive nitrogen
were found in the clay (2–0.1 μm) and silt fractions (63–2 μm), and the lowest were found in the coarse sand fraction (2,000–250 μm).
The phospholipid fatty acid analyses (PLFA) and denaturing gradient gel electrophoresis (DGGE) separation of 16S rRNA gene
fragments revealed that the microbial biomass within the clay fraction was predominantly due to soil bacteria. In contrast,
a high percentage of fungal-derived PLFA 18 : 2ω6 was found in the coarse sand fraction. Bacterial residues such as muramic
acid accumulated in the finer fractions in relation to fungal residues. The fractions also differed with respect to substrate
utilization: Urease was located mainly in the <2 μm fraction, alkaline phosphatase and arylsulfatase in the 2–63 μm fraction,
and xylanase activity was equally distributed in all fractions. Heavy metal pollution significantly decreased the concentration
of ninhydrin-reactive nitrogen of soil microorganisms in the silt and clay fraction and thus in the bulk soil. Soil enzyme
activity was reduced significantly in all fractions subjected to heavy metal pollution in the order arylsulfatase >phosphatase
>urease >xylanase. Heavy metal pollution did not markedly change the similarity pattern of the DGGE profiles and amino sugar
concentrations. Therefore, microbial biomass and enzyme activities seem to be more sensitive than 16S rRNA gene fragments
and microbial amino-sugar-N to heavy metal treatment.
Received: 21 January 2000 相似文献
9.
不同有机肥源对土壤微生物生物量及花生产量的影响 总被引:15,自引:1,他引:14
通过盆栽试验,采用平板计数法和DGGE分析法,研究施用化肥与不同来源的有机肥对土壤微生物生物量及花生产量的影响.结果表明,施肥均显著提高了花生的经济产量与生物产量,其中以施用麸酸有机复混肥处理最高;土壤中细菌、真菌、放线菌总量以施用鸡粪处理最高,其他处理差别不大;土壤微生物总DNA提取、PCR扩增及其产物DGGE分析表明,施用各品种有机肥较不施肥与施用化肥促进了土壤某些微生物量的提高,而施用不同有机肥品种促使不同种类微生物量的提高.故不同有机肥源对土壤微生物生物量乃至其多样性特征均产生影响. 相似文献
10.
11.
Weixing Liu Wenhua Xu Yi Han Changhui Wang Shiqiang Wan 《Biology and Fertility of Soils》2007,44(2):259-268
Temporal dynamics of microbial biomass and respiration of soil and their responses to topography, burning, N fertilization,
and their interactions were determined in a temperate steppe in northern China. Soil microbial indices showed strong temporal
variability over the growing season. Soil microbial biomass C (MBC) and N (MBN) were 14.8 and 11.5% greater in the lower than
upper slope, respectively. However, the percentage of organic C present as MBC and the percentage of total N present as MBN
were 16.9 and 26.2% higher in the upper than lower slope, respectively. Neither microbial respiration (MR) nor metabolic quotient
(qCO2) was affected by topography. Both MBC and MBN were increased by burning, on average, by 29.8 and 14.2% over the growing season,
and MR and qCO2 tended to reduce depending on the sampling date, especially in August. Burning stimulated the percentage of organic C present
as MBC and the percentage of total N present as MBN in the upper slope, but did not change these two parameters in the lower
slope. No effects of N fertilization on soil microbial indices were observed in the first growing season after the treatment.
Further research is needed to study the long-term relationships between changes in soil microbial diversity and activity and
plant community in response to burning and N fertilization. 相似文献
12.
不同施肥模式对设施菜田土壤微生物量碳、氮的影响 总被引:5,自引:4,他引:5
13.
Conservation tillage, and especially no-tillage, induce changes in the distribution of organic pools in the soil profile.
In long-term field experiments, marked stratification of the total soil microbial biomass and its activity have been observed
as consequence of the application of no-tillage to previously tilled soils. Our objective was to study the evolution of the
total and active soil microbial biomass and mineralized C in vitro during the first crop after the introduction of no-tillage
to an agricultural soil. The experiment was performed on a Typic Hapludoll from the Argentinean Pampa. Remaining plant residues,
total and active microbial biomass and mineralized C were determined at 0–5 cm and 5–15 cm depths, at three sampling times:
wheat tilling, silking and maturity. The introduction of no-tillage produced an accumulation of plant residues in the soil
surface layer (0–5 cm), showing stratification with depth at all sampling dates. Active microbial biomass and C mineralization
were higher under no-tillage than under conventional tillage in the top 5 cm of the profile. The total soil microbial biomass
did not differ between treatments. The active soil biomass was highly and positive correlated with plant residues (r
2=0.617;P<0.01) and with mineralized C (r
2=0.732;P<0.01). Consequently, the active microbial biomass and mineralized C reflected immediately the changes in residue management,
whereas the total microbial biomass seemed not to be an early indicator of the introduction of a new form of soil management
in our experiment.
Received: 23 February 1999 相似文献
14.
We investigated the effects of converting forest to savanna and plough land on the microbial biomass in tropical soils of India. Conversion of the forest led to a significant reduction in soil organic C (40–46%), total N (47–53%), and microbial biomass C (52–58%) in the savanna and the plough land. Among forest, savanna, and plough land, basal soil respiration was maximum in the forest, but the microbial metabolic quotient (qCO2 was estimated to be at a minimum in the forest and at a maximum in the plough land. 相似文献
15.
Influence of soil properties on microbial populations, activity and biomass in humid subtropical mountainous ecosystems of India 总被引:1,自引:0,他引:1
Microbial populations, biomass, soil respiration and enzyme activities were determined in slightly acid organic soils of
major mountainous humid subtropical terrestrial ecosystems, along a soil fertility gradient, in order to evaluate the influence
of soil properties on microbial populations, activity and biomass and to understand the dynamics of the microbial biomass
in degraded ecosystems and mature forest. Although the population of fungi was highest in the undisturbed forest (Sacred Grove),
soil respiration was lowest in the 7-year-old regrowth and in natural grassland (approximately 373 μg g–1 h–1). Dehydrogenase and urease activities were high in "jhum" fallow, and among the forest stands they were highest in the 7-year-old
regrowth. Microbial biomass C (MBC) depended mainly on the organic C status of the soil. The MBC values were generally higher
in mature forest than in natural grassland, 1-year-old jhum fallow and the 4-year-old alder plantation. The MBC values obtained
by the chloroform-fumigation-incubation technique (330–1656 μg g–1) did not vary significantly from those obtained by the chloroform-fumigation-extraction technique (408–1684 μg g–1), however, the values correlated positively (P<0.001). The enzyme activities, soil respiration, bacterial and fungal populations and microbial biomass was greatly influenced
by several soil properties, particularly the levels of nutrients. The soil nutrient status, microbial populations, soil respiration
and dehydrogenase activity were greater in Sacred Grove, while urease activity was greater in grassland.
Received: 14 October 1998 相似文献
16.
Estimating the active and total soil microbial biomass by kinetic respiration analysis 总被引:1,自引:0,他引:1
A model describing the respiration curves of glucose-amended soils was applied to the characterization of microbial biomass.
Both lag and exponential growth phases were simulated. Fitted parameters were used for the determination of the growing and
sustaining fractions of the microbial biomass as well as its specific growth rate (μ
max). These microbial biomass characteristics were measured periodically in a loamy silt and a sandy loam soil incubated under
laboratory conditions. Less than 1% of the biomass oxidizing glucose was able to grow immediately due to the chronic starvation
of the microbial populations in situ. Glucose applied at a rate of 0.5 mg C g–1 increased that portion to 4–10%. Both soils showed similar dynamics with a peak in the growing biomass at day 3 after initial
glucose amendment, while the total (sustaining plus growing) biomass was maximum at day 7. The microorganisms in the loamy
silt soil showed a larger growth potential, with the growing biomass increasing 16-fold after glucose application compared
to a sevenfold increase in the sandy loam soil. The results gained by the applied kinetic approach were compared to those
obtained by the substrate-induced respiration (SIR) technique for soil microbial biomass estimation, and with results from
a simple exponential model used to describe the growth response. SIR proved to be only suitable for soils that contain a sustaining
microbial biomass and no growing microbial biomass. The exponential model was unsuitable for situations where a growing microbial
biomass was associated with a sustaining biomass. The kinetic model tested in this study (Panikov and Sizova 1996) proved
to describe all situations in a meaningful, quantitative and statistically reliable way.
Received: 19 July 1999 相似文献
17.
The present research was conducted to determine the relationship between the degradation of rimsulfuron and soil microbial
biomass C in a laboratory-incubated clay loam soil (pH=8.1; organic matter=2.1%) under different conditions and at different
initial dosages (field rate, 10 and 100 times the field rate). The half-life values varied between 0.4 and 103.4 days depending
on temperature, soil moisture and initial dose. Evidence suggested that rimsulfuron could pose environmental risks in cold
and dry climatic conditions. Significant decreases in microbial biomass C content in rimsulfuron-treated soil, compared to
untreated soil, were observed initially, especially at higher temperatures and low moisture levels, but never exceeded 20.3%
of that in control soil. The microbial biomass C content then returned to initial values at varying times depending on incubation
conditions. The relationship between herbicide degradation and microbial biomass C content gave parabolic curves (P<0.005 in all cases) under all conditions tested. Generally, maximum biomass C decrease coincided with the decrease in the
concentration of rimsulfuron to about 50% of the initial dose, except at 10 °C and 100×, when biomass began to recover as
early as 65–70% of the initial dose. The final equations could be useful to deduce the decrease of soil microbial biomass
in relation to herbicide concentration. From the degradation kinetics of the herbicide, the time required to reach this decrease
can also be calculated.
Received: 19 July 1999 相似文献
18.
As part of a broader study, the aim of which is to identify soil factors that might be associated with yield decline of sugar
cane, microbial biomass and protease activities were examined in soil samples collected from seven paired old and new land
sites in three cane-growing districts of north Queensland. No consistent changes in soil protease activities were observed,
although some sites exhibited specific effects, as a result of extensive periods of sugar cane monoculture. Soil microbial
biomass, however, was significantly lower in those soils where sugar cane had been grown for extended periods. The implications
of a lowering of soil microbial biomass on sugar cane yields and sustainability are discussed.
Received: 24 June 1997 相似文献
19.
Microbial biomass,activity, and organic matter accumulation in soils contaminated with heavy metals 总被引:8,自引:0,他引:8
Chemical characteristics and some parameters related to biological components were determined in 16 soils from a fairly homogeneous area in the north of Italy, contaminated with different levels of heavy metals. Correlation analysis of the parameters studied showed close positive relationships among the metals and with the organic C content in the soils studied. Negative relationships were observed among the heavy metals, soil respiration, and the ratio between evolved CO2–C and microbial biomass C per unit time (specific respiratory activity). This was ascribed to an adverse heavy metal effect on the soil microflora, which appeared to increase the accumulation of organic matter as the heavy metal content increased, probably because the biomass was less effective in mineralising soil organic matter under these conditions. 相似文献
20.
长期平衡施肥对潮土微生物活性和玉米养分吸收的影响 总被引:2,自引:0,他引:2
利用中国科学院封丘农业生态实验站农田生态系统养分平衡长期定位试验地,研究氮磷钾平衡施肥(NPK)与缺素施肥(NK、PK、NP)对土壤微生物生物量、酶活性、呼吸强度以及玉米养分吸收的影响。结果发现,与不施肥对照(CK)相比,NPK处理玉米根系与茎叶生物量、籽粒产量以及植株氮磷钾吸收量均大幅提高,NP处理次之,PK与NK处理则无显著影响;同一处理玉米茎叶与根系养分含量接近,而籽粒的全氮和全磷含量较高、全钾含量偏低;与NPK处理相比,缺施氮、磷或钾肥均直接导致玉米植株相应养分的明显亏缺或其他养分的过量富集,但在根系、茎叶和籽粒部位的累积情况存在一定差异。与CK相比,所有施加磷肥的处理(NPK、NP、PK)土壤微生物生物量(碳、氮、磷)、脱氢酶、转化酶、脲酶与碱性磷酸酶活性以及土壤微生物代谢活性和土壤基础呼吸强度均显著升高(p<0.05),土壤微生物代谢熵则显著下降(p<0.05),而缺施磷肥的NK处理除显著提高脲酶活性外(p<0.05),对其他指标均无显著影响。结果表明,氮磷钾平衡施肥在促进土壤微生物繁育和保育微生物代谢活性以及促进作物生长和保证养分吸收等方面显得非常重要,而缺素施肥中以缺施磷肥的不利影响最为突出。 相似文献